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Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2) Wuying CHU~3.Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2) Yongwei WEI~2 Lian YU~2* 1 Department of Biomedical Engineering,Zhejiang University,Hangzhou 3.0027,china 2 institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 3.0029,china {3.,chinese academy of Sciences,changsha 410125,china 4 Ningbo Oceanography and Fishery institute,Ningbo 3.5012,china DepartmentofBiomedicalEngineering ZhejiangUniversityHangzhou310027China instituteofPreventiveVeterinaryMedicine ZhejiangUniversityHangzhou310029China instituteofsubtropicalagriculture ChineseAcademyofSciencesChangsha

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 214 amino acidresidues with a predicted molecular weight of 23.3.*** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2%)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL21(DE3..Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

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Possible mechanism of the effect of convection over Asian-Australian “land bridge” on the East Asian summer monsoon onset

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Science china Earth Sciences 2006年第11期49卷 1223-1232页

作者： HE Jinhai1, WEN Min2, DING Yihui3.& ZHANG Renhe2 1. Jiangsu Key Laboratory of Meteorological Disaster (KLME), Nanjing institute of Meteorology, Nanjing University of Informa- tion Science and Technology, Nanjing 210044, china 2. State Key Laboratory of Severe Weather (LaSW), chinese {3. of Meteorological Sciences, Beijing 100081, china 3. National Climate Center, chinese Meteorological Administration, Beijing 100081, china 1. Jiangsu Key Laboratory of Meteorological Disaster (KLME) Nanjing Institute of Meteorology Nanjing University of Information Science and Technology Nanjing 210044 China 2. State Key Laboratory of Severe Weather (LaSW) Chinese Academy of Meteorological Sciences Beijing 100081 China 3. National Climate Center Chinese Meteorological Administration Beijing 100081 China

The Asian-Australian “land bridge” is an area with the most vigorous convection in Asian monsoon region in boreal spring, where the onset and march of convection are well associated with the onset of East Asian summer monsoon. The convection occurs over Indo-china Peninsula as early as mid-April, which exerts critical impact on the evolution of monsoon circulation. Before mid-April there are primarily sensible heatings to the atmosphere over Indo-china Peninsula and Indian Peninsula, so the apparent heating ratios over them decrease with height. However, after mid-April it changes into latent heating over Indo-china Peninsula due to the onset of convection, and the apparent heating ratio increases with height in mid- and lower troposphere. The vertical distribution of heating ratio and its differences between Indo-china Peninsula and Indian Peninsula are the key factors leading to the splitting of boreal subtropical high belt over the Bay of Bengal. Such mechanism is strongly supported by the fact that the evolution of the vertical heating ratio gradient above Indo-china Peninsula leads that of 850 hPa vorticity over the Bay of Bengal. Convections over Indo-china Peninsula and its surrounding areas further increase after the splitting. Since then, there is a positive feedback lying among the convective heating, the eastward retreat of the subtropical high and the march of monsoon, which is a possible mechanism of the advance of summer monsoon and convection from Indo-china Peninsula to South china Sea.

关键词： Asian-Australian 'land bridge', tropical convection, Indo-china Peninsula, the splitting of boreal subtropical high belt, East Asian summer monsoon onset.

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A new method for spatio-temporal prediction of rainfall-induced landslide

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Science china Earth Sciences 2006年第4期49卷 421-430页

作者： DING Jixin1,2, YANG Zhifa2, SHANG Yanjun2, ZHOU Shenghua1 & YIN Juntao3.1. china Non-ferrous Metals Resource Geological Survey, Beijing 100012, china 2. institute of Geology and Geophysics, {3. academy of Sciences, Key Laboratory of Engineering Geomechanics, Beijing 100029, china 3. School of Geoscience & Environmental Engineering, Central South University, changsha 410083. china 1. china Non-ferrous Metals Resource Geological Survey Beijing 100012 China 2. institute of Geology and Geophysics Chinese Academy of Sciences Key Laboratory of Engineering Geomechanics Beijing 100029 China 3. School of Geoscience & Environmental Engineering Central South University Changsha 410083 China

Geological condition and rainfall are two most principal conditions inducing landslides in the Chongqing region. By analyzing the forming conditions of rainfall-induced landslides, a new method for spatio-temporal prediction of rainfall-induced landslide is brought forward on the basis of grading and overlapping geological condition and rainfall factor in this paper. At first, semi-quantitative assessment and grading for the geological condition of a certain area or slope can be carried out with the multi-factor interactive matrix. Then the severity of rainfall in that area is grading according to the maximum daily rainfall and the total rainfall in a rainfall course. Finally, the “landslide probability judgement factor” can be worked out through grading and overlapping “geological condition influenc- ing factor” and “rainfall influencing factor”, by which the landslide can be graded into 4 grades, they are landslide extremely easily happening, landslide easily happening, landslide difficultly happening and landslide hardly ever happening respectively. More accurate spatio-temporal prediction of rain- fall-induced landslides can come true on the ground of detailed geological survey of some dangerous slopes in an area and more precise weather forecast. Finally, the reliability and feasibility of carrying out the spatio-temporal prediction of rainfall-induced landslides with the method of “two factors” grading and overlapping are validated by the example of Jipazi landslide.

关键词： rainfall-induced landslide, geological condition, rainfall, two factors, grading and overlapping, interactive matrix, spatio-temporal prediction.

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CONVERGENCE OF (0,1,2,3. INTERPOLATION ON AN ARBITRARY SYSTEM OF NODES

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Journal of Computational Mathematics 2001年第2期19卷 151-156页

作者： Ying-guang Shi (institute of Computation, Hunan Normal University, changsha 410081, china) (institute of Computational Mathematics and Scientific/Engineering Computing, academy of Mathematics and Systems Sciences, chinese academy of Sciences, Beijing 100 湖南师范大学湖南长沙 410081

Provides information on a study which simplified and improved convergence of (0,1,2,3. interpolation on an arbitrary system of nodes. Definitions and notations; Results; Conjectures.

关键词： Hermite interpolation Hermite-Fejer interpolation convergence

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Sea-salt aerosol transport patterns over the Northern Hemisphere inferred from two subarctic ice core records

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Science china Earth Sciences 2005年第4期48卷 576-584页

作者： YAN Yuping1,2, KANG Shichang2,3.5, SUN Junying4,5, Paul A. Mayewski2 & QIN Dahe5 1. National Climate Center, china Meteorological Administration, Beijing 100081, china 2. Climate Change institute, University of Maine, Orono, ME 04469, USA 3. institute of Tibetan Plateau Research, chinese academy of Sciences, Beijing 100085, china 4. chinese academy of Meteorological Sciences, Beijing 100081, china 5. Key Laboratory of Ice Core and Cold Regions Environment, Cold and Arid Environmental and Engineering Research institute, chinese academy of Sciences, Lanzhou 73.000, china 1. National Climate Center China Meteorological Administration 100081 Beijing China 2. Climate Change institute University of Maine 04469 Orono ME USA 3. institute of Tibetan Plateau Research Chinese Academy of Sciences 100085 Beijing China 4. Key Laboratory of Ice Core and Cold Regions Environment Cold and Arid Environmental and Engineering Research Institute Chinese Academy of Sciences 730000 Lanzhou China 5. chinese academy of Meteorological Sciences 100081 Beijing China

Atmospheric circulation reconstruction based on glaciochemical records requires knowledge of chemical concentration controls, such as source, transport pathway and strength. To gain insight into these processes, the relationships between glaciochemical records from two Northern Hemisphere sites (Mt. Logan in Yukon Territory and 20D in southern Greenland) and instrumental sea level pressure (SLP) series are investigated. Calibrations between Mt. Logan sea-salt sodium (ssNa+) concentration and SLP series show that ssNa+ concentrations are closely correlated with the autumntime (SON) Aleutian Low and the summertime (JJA) North Pacific subtropical High. Both the deepened Aleutian Low and enhanced North Pacific Sub- tropical High strengthen the transport of sea-salt aerosols from the North Pacific to the Mt. Logan region. Calibrations between 20D ssNa+ concentrations and SLP series indicate that ssNa+ concentrations are closely related to the wintertime (Jan.) Icelandic Low. A deepening of the Icelandic Low strengthens winter storms and frequent cyclogenesis over the North Atlantic and pushes more sea-salt laden air masses to the Greenland ice sheet. Therefore, ice core ssNa+ records from the Mt. Logan region can be considered as a proxy for reconstructing the au- tumntime Aleutian Low and summertime North Pacific subtropical High, and the ssNa+ records from Greenland ice core (20D) may provide a proxy for reconstructing the wintertime Icelandic Low.

关键词： sea-salt aerosol, ice core records, sea level pressure, subarctic regions.

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Modeling the impact of Australian Plate drift on Southern Hemisphere climate and environment

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chinese Science Bulletin 2005年第14期50卷 1495-1502页

作者： ZHOU Botao1,5, ZHAO Ping2, JIAN Zhimin3.& HE Jinhai4 1. Nansen-Zhu International Research Center, institute of Atmospheric Physics, chinese academy of Sciences, Beijing 100029, {3. 2. institute of Climate and Environment, chinese academy of Meteoro- logical Sciences, Beijing 100081, {3. 3. Laboratory of Marine Geology, Tongji University, Shanghai 200092, {3. 4. Department of Atmospheric Sciences, Nanjing institute of Meteorol- ogy, Nanjing 210044, {3. 5. Graduate School of the chinese academy of Sciences, Beijing 10003., {3. Nansen-Zhu International Research Center Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Graduate School of the chinese academy of Sciences Beijing 100039 China institute of Climate and Environment Chinese Academy of Meteorological Sciences Beijing 100081 China Laboratory of Marine Geology Tongji University Shanghai 200092 China Department of Atmospheric Sciences Nanjing Institute of Meteorology Nanjing 210044 China

Using a global atmosphere-ocean coupled model with the present-day and 14 MaB.P. oceanic topogra- phy respectively, two experiments are implemented to inves- tigate the effect of different locations of Australian Plate on the atmospheric circulation in middle-high latitudes of the Southern Hemisphere. The results show that when Austra- lian Plate lay south at 14 MaB.P., both anticyclone circula- tions in the subtropical oceans and cyclone circulation around 60°-70°S are strengthened. subtropical highs and circumpolar low pressure appear stronger, which results in much stronger Antarctic Oscillation and shorter period of Antarctic Oscillation Index (AOI) at 14 MaB.P. The rainfall and the surface air temperature also change correspondingly. The precipitation decreases around 40°S and increases around 60°-70°S, and the surface air temperature rises in high latitudes of the South Pacific and descends over the Weddell Sea and its north side. Besides, due to the changes of the temperatures and winds, Antarctic sea ice coverage also changes with its increasing in the Ross Sea and its west re- gions and decreasing in the Weddell Sea.

关键词：半球气候地球环球澳大利亚漂流物海洋地形学

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Enhancing disease resistances of Super Hybrid Rice with four antifungal genes

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Science china(Life Sciences) 2007年第1期50卷 31-39页

作者： ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3. YUAN LongPing2 & LI BaoJian1 1 Biotechnology Research Center, Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, china 2 china National Hybrid Rice R&D Center, Hunan Hybrid Rice Research Center, changsha 410125, china 3.institute of subtropical agriculture, the chinese academy of Sciences, changsha 410125, china 1. Biotechnology Research Center Key Laboratory of Gene Engineering of Ministry of Education Sun Yat-sen University Guangzhou 510275 China 2. china National Hybrid Rice R&D Center Hunan Hybrid Rice Research Center Changsha 410125 China 3. institute of subtropical agriculture the Chinese Academy of Sciences Changsha 410125 China

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘93.1’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘93.1’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).

关键词： Super Hybrid Rice multi-gene transformation rice blast rice false smut rice kernel smut anti-fungi disease resistance

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Phosphorus Transfer and Distribution in a Soybean-Citrus Intercropping System

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Pedosphere 2006年第4期16卷 435-443页

作者： ZHOU Wei-Jun WANG Kai-Rong ZHANG Yang-Zhu YIN Li-Chu LI He-Song College of Resources and Environment Hunan Agricultural University Changsha 410128 （China） institute of subtropical agriculture Chinese Academy of Sciences Changsha 4 10125 （China）

A mini-plot field experiment was conducted on a loamy clay Oxisol to compare and evaluate P absorption and transfer in plant organs and P movement in soil profile at three P application depths under the soybean-citrus intercropping versus the monoculture using a ^3.p tracer technique. Total P absorption （Pt） by soybean and P accumulation （Pa） in soybean organs decreased significantly （P 〈 0.05） under the intercropping in contrast to the monoculture. With intercropping, when ^3.p was applied in topsoil （15 cm soil layer）, total ^3.p absorption （^3.pt） in soybeans was significantly lower （P 〈 0.05）, but when ^3.p was applied to deeper soil layers （3. or 55 cm soil layer）, ^3.pt in soybeans was significantly greater （P 〈 0.05）. The percentage of P in leaves to total P （Pa/Pt） and 3.p in leaves to total ^3.p （^3.pa/^3.pt） for soybean were ≥ 25% and those of root ≥ 12%. When P was applied ia topsoil and 55 cm soil layer, no significant differences were found between intercropping and monoculture for Pt of citrus. The P absorbed by citrus was transferred rapidly to the growing organs of aboveground during the experiment, and the speed of transferring to the growing organs slowed when P was applied to the deeper soil layers. In intercropping, P mobility was heightened in the soil profile, and P in deeper soil layers moved up to topsoil more rapidly.

关键词： agroforestry ecosystem citrus monoculture, ^3.p fate soybean

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Chronological and geochemical studies of granite and enclave in Baimashan pluton, Hunan, South china

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Science china Earth Sciences 2007年第11期50卷 1606-1627页

作者： CHEN WeiFeng1, CHEN PeiRong1, HUANG HongYe2, DING Xing1,3.& SUN Tao1 1 State Key Laboratory of Mineral Deposit Research, Department of Earth Sciences, Nanjing University, Nanjing 210093. china 2 Research institute No.23., CNNC, changsha 410011, china 3.Guangzhou institute of Geochemistry, chinese academy of Sciences, Guangzhou 510640, china 1. State Key Laboratory of Mineral Deposit Research Department of Earth Sciences Nanjing University Nanjing 210093 China 2. Research institute No.23. CNNC Changsha 410011 China 3. Guangzhou institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

Zircon LA-ICP-MS U-Pb dating reveals that the Baimashan Pluton is composed mainly of late Indosinian (204.5±2.8 Ma-209.2±3.8 Ma) biotite granodiorites/monzonitic granites (LIGs) and early Yanshanian (176.7±1.7 Ma) two-micas monzonitic granites (EYGs), and the coeval (203.2±4.5 Ma-205.1±3.9 Ma) mafic microgranular enclaves (MMEs) are generally found in the former. In addition, the ages of cores within zircons from LIGs and MMEs ranging from 221.4±4.0 Ma to 226.5±4.1Ma provide evidence of multistage magma intrusion during Indosinian in the study area. Measured 3.10±20.6 Ma of inherited zircon age suggests that there may be recycling Archaean curstal material in existence in this area. LIGs and EYGs share some similar geochemical features: subalkaline and peraluminous granites, enrichment of Th, U, K, Ta, Zr, Hf and LREE but depletion of Ba, Nb, P, Ti and Eu, low εNd(t) values but high (87Sr/86Sr)i ratios, and old T2DM (ca. 1.9-2.0 Ga). The behaviors of incompatible elements and REE are mainly dominated by fractional crystallization of plagioclase, K-feldspar, ilmenite and apatite, but that of Sr isotope mainly controlled by EC-AFC. They are crust-sourced and derived from partial melting of paleo-Proterozoic metagreywackes and related to biotite dehydration melting. LIGs are formed in post-collisional tectonic setting as crustal local extension and thinning during late Indosinian. But EYGs may be evolved products of congeneric granitic magma with LIGs formed in late Indoinian, which were emplaced again when crust underwent extensive thinning and extension in post-orogenic tectonic setting during Yanshanian in SC after undergoing EC-AFC. MMEs should be cognate enclaves and derived from liquid immiscibility of host magma.

关键词： granite,mafic microgranular enclave,zircon LA-ICP-MS dating,lithogenesis,EC-AFC

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Latest progress on interactions between VLF/ELF waves and energetic electrons in the inner magnetosphere

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Science china Earth Sciences 2010年第3期53卷 317-326页

作者： XIAO FuLiang1,2, ZONG QiuGang3. SU ZhenPeng4, TIAN Tian3.& ZHENG HuiNan4 1 School of Physics and Electronic Sciences, changsha University of Science and Technology, changsha 410004, china 2 State Key Laboratory of Space Weather, {3. academy of Sciences, Beijing 100190, china 3.institute of Space Physics and Applied Technology, Peking University, Beijing 100871, china 4 CAS Key Lab for Basic Plasma Science, School of Earth and Space Sciences, University of Science and Technology of china, Hefei 23.026, china 1. School of Physics and Electronic Sciences Changsha University of Science and Technology Changsha 410004 China2. State Key Laboratory of Space Weather Chinese Academy of Sciences Beijing 100190 China3. Institute of Space Physics and Applied Technology Peking University Beijing 100871 China4. CAS Key Lab for Basic Plasma Science School of Earth and Space Sciences University of Science and Technology of China Hefei 230026 China

Interactions between very/extremely low frequency (VLF/ELF) waves and energetic electrons play a fundamental role in dynamics occurring in the inner magnetosphere. Here, we briefly discuss global properties of VLF/ELF waves, along with the variability of the electron radiation belts associated with wave-particle interactions and radial diffusion. We provide cases of electron loss and acceleration as a result of wave-particle interactions primarily due to such waves, and particularly some preliminary results of 3. evolution of phase space density from our currently developing 3. code. We comment on the existing mechanisms responsible for acceleration and loss, and identify several critical issues that need to be addressed. We review latest progress and suggest open questions for future investigation.

关键词： wave-particle interaction VLF/ELF waves energetic electrons acceleration and loss inner magnetosphere

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