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An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli

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Acta Biochimica et Biophysica sinica 2004年第8期36卷 571-576页

作者： Li-Qing XIAO, Ai-Hua LIU, and Yong-Lian ZHANG State Key Laboratory of molecular Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200031, china State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family.

关键词： Bin1b epididymis β-defensin double-copy protein polyclonal antiserum

Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

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Acta Biochimica et Biophysica sinica 2004年第10期36卷 673-680页

作者： Chun-Hui HOU, Jian HUANG, and Ruo-Lan QIAN* State Key Laboratory of molecular Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200031, china State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3028 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3004 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA

Theoretical research progress in complexity of complex dynamical networks

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Progress in Natural Science:Materials International 2007年第7期17卷 761-774页

作者： Fang Jinqing(Joint Complex Network Research Group,china institute of atomic Energy,Beijing 102413,china) [a]Joint Complex Network Research Group China Institute of Atomic Energy Beijing China

This article reviews the main progress in dynamical complexity of theoretical models for nonlinear complex networks proposed by our Joint Complex Network Research Group(JCNRG).The topological and dynamical properties of these theoretical models are numerically and analytically *** findings are useful for understanding and deeply studying complex networks from macro- scopic to microscopic levels and have a potential of applications in real-world networks.

关键词： Harmonions unifying hybrid preferential model a large unifying hybrid network model quantum interference network nonowire networks generalized local-world model non-local links model evolving model of weighted networks small-world network with the unchanged degree

VISCOELASTIC BEHAVIOR OF THE NON-HOOKE DEFORMATION BEFORE YIELDING IN UNIAXIAL DRAWING OF AMORPHOUS POLY(ETHYLENE TEREPHTHALATE)

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Chinese Journal of Polymer Science 2001年第4期19卷 399-405页

作者： Zhen-hua Yuan De-yan Shen Ren-yuan Qian Polymer Physics Laboratory Center for molecular Science, institute of Chemistry Chinese Academy of sciences, Beijing 100080 china Chinese Acad Sci Inst Chem Ctr Mol Sci Polymer Phys Lab Beijing 100080 Peoples R China

Viscoelastic behavior of the non-Hooke deformation of amorphous PET film before yield was investigated in the temperature region 74-80.5 degreesC around the glass transition temperature. The film specimen was drawn to yield point followed by unloading to zero stress, then the residual deformation was held constant; while the subsequent evolution of the stress was recorded. An induction period was found in the course of stress evolution followed by a stress step-increase. The induction period decreases with increasing drawing temperature with an activation energy of 1.10 MJ/mol.K, which is attributed to the time needed for the relaxation of rubbery deformation through cooperative internal rotations. At temperatures lower than 74 degreesC, there is no stress increase or the induction period becomes too long to be observed. Thus the nature of anelasticity in the non-Hooke region before yielding is attributed to stress induced rubbery deformation. The experimental results are interpreted in terms of Perez' theological model of a series connected Hooks spring and a Voigt element consisting of a parallel connected elastic spring and a dashpot.

关键词： pre-yield deformation PET uniaxial drawing non-Hooke deformation viscoelasticity stress induced rubbery deformation

An N/4 fixed-point duality quantum search algorithm

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中国科学：物理学、力学、天文学英文版 2010年第9期53卷 1765-1768页

作者： HAO Liang1,LIU Dan2 & LONG GuiLu1,3 1Key Laboratory for atomic and molecular Nanosciences and Department of Physics,Tsinghua University,Beijing 100084,china 2School of sciences,Dalian Nationalities University,Dalian 116600,china 3Tsinghua National Laboratory for Information Science and Technology,Beijing 100084,china 1. Key Laboratory for atomic and molecular Nanosciences and Department of Physics Tsinghua University Beijing 100084 China2. School of Sciences Dalian Nationalities University Dalian 116600 China3. Tsinghua National Laboratory for Information Science and Technology Beijing 100084 China

Here a fixed-point duality quantum search algorithm is *** algorithm uses iteratively non-unitary operations and measurements to search an unsorted *** the marked item is found,the algorithm stops *** algorithm uses a constant non-unitary operator,and requires N/4 steps on average(N is the number of data from the database) to locate the marked *** implementation of this algorithm in a usual quantum computer is also demonstrated.

关键词： fixed-point search algorithm duality quantum computing the N4 duality search algorithm

陨石、超基性岩、花岗岩及其主矿物中某些微量元素的仪器中子活化分析

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地球化学 1976年第2期5卷 133-143页

作者： METEOEITE ANALYSIS GROUPinstitute of atomic Energy, academia sinica Guiyang institute of Geochemistry, academia sinica 中国科学院原子能研究所陨石分析组中国科学院贵阳地球化学研究所陨石分析组

研究地球物质中化学元素的分布、迁移和转变的规律，进一步探讨地球的形成与演化过程是地球化学的主要课题之一。球粒陨石的平均化学组成可能代表着形成太阳系的太阳星云的平均化学组成，也是组成地球的初始物质，而地壳上的各种岩浆岩... 详细信息

研究地球物质中化学元素的分布、迁移和转变的规律，进一步探讨地球的形成与演化过程是地球化学的主要课题之一。球粒陨石的平均化学组成可能代表着形成太阳系的太阳星云的平均化学组成，也是组成地球的初始物质，而地壳上的各种岩浆岩都是从地球的初始物质经过熔融、调整、演化而逐渐形成的。我们通过对普通球粒陨石、超基性岩。

关键词：地球化学球粒陨石化学组成超基性岩花岗岩微量元素中子活化分析

Studies on the Temperature Effect on Bacteriorhodopsin of Purple and Blue Membrane by Fluorescence and Absorption Spectroscopy

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Acta Biochimica et Biophysica sinica 2006年第10期38卷 691-696页

作者： Lan-Ying CHENG~(1,2) Yue ZHANG~3 Shi-Gui LIU~1 Kun-Sheng HU~3 Kang-Cheng RUAN~2* 1 College of Life Science,Sichuan University,Chengdu 610064,china 2 State Key Laboratory of molecular Biology,institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences,Chinese Academy of sciences,Shanghai 200031,china 3.institute of Biophysics,academia sinica,Beijing 100101,china State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Fluorescence and absorption spectra were used to study the temperature effect on theconformation of bacteriorhodopsin (bR) in the blue and purple membranes (termed as bRb and bRprespectively).The maximum emission wavelengths of tryptophan fluorescence in both proteins at roomtemperature are 340 nm,and the fluorescence quantum yield of bRb is about 1.4 fold higher than that of *** temperature increases,the tryptophan fluorescence of bRb decreases,while the tryptophan fluorescenceof bRp *** binding study of extrinsic fluorescent probe bis-ANS indicated that the probe can bindonly to bRb,but not to *** results suggest that significant structural difference existed between bRband *** was also found that both kinds of bR are highly thermal *** maximum wavelength of theprotein fluorescence emission only shifted from 340 nm to 346 nm at 100℃.More interestingly,as tempera-ture increased,the characteristic absorption peak of bRb at 605 nm decreased and a new absorption peak at380 nm *** transition occurred at a narrow temperature range (65℃-70℃).These facts indicatedthat an intermediate can be induced by high *** phenomenon has not been reported before.

关键词： bacteriorhodopsin purple membrane blue membrane fluorescence and absorption spectroscopy

A　STATISTICAL　THEORY　FOR　THE　BIO-PHOTON　EMISSION　OF　THE　LIVING　SYSTEMS

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原子与分子物理学报 1997年第1期 132-140页

作者： Pang Xiaofeng International Centre for Material Physics, academia sinica Dept. of Physics, Southwest institute for Nationalities The Institue of atomic and molecular Physics, Sichuan Union UniversityShenyang Chengdu Chengdu110015 610051 610065

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ＡＳＴＡＴＩＳＴＩＣＡＬＴＨＥＯＲＹＦＯＲＴＨＥＢＩＯ－ＰＨＯＴＯＮＥＭＩＳＳＩＯＮＯＦＴＨＥＬＩＶＩＮＧＳＹＳＴＥＭＳＰａｎｇＸｉａｏｆｅｎｇＩｎｔｅｒｎａｔｉｏｎａｌＣｅｎｔｒｅｆｏｒＭａｔｅｒｉａｌＰｈｙｓｉｃｓ，ＡｃａｄｅｍｉａＳｉｎｉｃａＤ...

关键词： Bio photon emission Molecules Negative entropy Living things Statistical theory of non equilibrum state Bio self organization

A Fast Algorithm for Phase Grating Preparation by Real Space Method

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Journal of Materials Science & Technology 2001年第2期17卷 240-242页

作者： Qiuxiang LIU and Yuanming WANG Laboratory of atomic Imaging of Solids, institute of Metal Research, Chinese Academy of sciences, Shengyang 110015, china Qibin YANG and Xiaohong YAN institute of Modern Physics, Xiangtan University, Xiangtan 411105, china Chinese Acad Sci Inst Met Res Atom Imaging Solids Lab Shenyang 110015 Peoples R China Xiangtan Univ Inst Modern Phys Xiangtan 411105 Peoples R China

Making use of this expression to calculate the phase grating in high resolution image simulation can greatly reduce the calculating time. In this paper, the derivation of the expression is introduced, and then the computer routine is explained in details. Finally the potential projection map of Mg44Rh7 along [001] direction is shown as an illustration. All operations are carried out in real space, so we call the calculation method as the real space method.

关键词： A Fast Algorithm for Phase Grating Preparation by Real Space Method