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Expressional Analysis of an EREBP Transcription Factor Gene OsEBP-89 in Rice

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 21-26页

作者： Hui SHEN and Zong-Yang WANG( The state Key laboratory of plant Molecular Genetics, Institute of plant Physiology & Ecology, Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences, Shanghai 200032, China ) The state Key laboratory of plant Molecular Genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China

OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza sativa). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem, seeds, flowers and leaves. Histochemical assay showed that β-glucuronidase (GUS) was expressed mainly in phloem of vascular tissues of the root and stem transition region (RST), basal part of sheath roots, stem node and basal part of adventitious roots, also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct (pNSG). A sequence region from -279 to -97 was found to play an important role for OsEBP-89 gene expression through promoter deletion assay. The possible function of OsEBP-89 gene was discussed.

关键词： OsEBP-89 gene rice EREBP subfamily transcription factor tissue expression

Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon

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Journal of Genetics and Genomics 2011年第12期38卷 593-601页

作者： Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun state Key laboratory of plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild plant(Rice), laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of plant Genetics and Breeding,China Agricultural university,Beijing 100193,China state Key laboratory of plant Physiology and Biochemistry National Centre for Evaluation of Agricultural Wild Plant (Rice) Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture Department of Plant Genetics and Breeding China Agricultural University Beijing 100193 China

Soil salinity is one of the major abiotic stresses affecting plant growth and crop *** the present study,salt tolerance at rice seedling stage was evaluated using 87 introgression lines（ILs）,which were derived from a cross between an elite indica cultivar Teqing and an accession of common wild rice（Oryza rufipogon Griff.）.Substantial variation was observed for four traits including salt tolerance score（STS）, relative root dry weight（RRW）,relative shoot dry weight（RSW） and relative total dry weight（RTW）.STS was significantly positively correlated with all other three traits.A total of 15 putative quantitative trait loci（QTLs） associated with these four traits were detected using single-point analysis,which were located on chromosomes 1,2,3,6,7,9 and 10 with 8%-26%explaining the phenotypic *** O. rufipogon-derived alleles at 13 QTLs（86.7%） could improve the salt tolerance in the Teqing *** QTL clusters affecting RRW, RSW and RTW were found on chromosomes 6,7,9 and 10,*** these four QTL clusters,a major cluster including three QTLs （qRRWIO,qRSWIO and qRTWIO） was found near the maker RM271 on the long arm of chromosome 10,and the ***-derived alleles at these three loci increased RRW,RSW and RTW with additive effects of 22.7%,17.3%and 18.5%,respectively,while the phenotypic variance explained by these three individual QTLs for the three traits varied from 19%to 26%.In addition,several salt tolerant ILs were selected and could be used for identifying and utilizing favorable salt tolerant genes from common wild rice and used in the salt tolerant rice breeding program.

关键词： Common wild rice(O.rufipogon Griff.) Introgression lines Salt tolerance Seedling stage Quantitative trait locus

The relationship between epithermal and low temperature mineralizations

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Chinese Science Bulletin 1999年第S2期44卷 226-227页

作者： HUA Renmin state Key laboratory of Mineral Deposit research, Nanjing university, Nanjing 210093, China state Key laboratory of Mineral Deposit research Nanjing University Nanjing 210093 China

THE term epithermal as a kind of hydrothermal mineralization was first used by Lindgren in 1933. It describes those ore-forming systems occurring at depths mainly less than 1 km, and at temperatures less than300℃. Epithermal deposits of Cenozoic ages are widely distributed in the Circum-Pacific belt, especiallyin the Southwest Pacific area. From the 1980s on, the study of epithermal ore deposits became an important field of mineral deposit research. Hayba et *** Hedenquist classified the epithermal systemas adularia-sericite or acid-sulphate types, and low sulphidation or high sulphidation types, *** low temperature mineralization, which was exemplified by the deposits in southwest China, refers tothe system at temperature mainly less than 200℃. Besides the distinction in temperature, however, thereexist many differences between epithermal and low temperature mineralizations.

关键词： epithermal mineralization low temperature.

Generation of fad2 transgenic mice that produce omega-6 fatty acids

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Science China(Life Sciences) 2009年第11期52卷 1048-1054页

作者： CHEN Qing1, LIU Qing2, WU ZhiFang1, WANG ZongYi3 & GOU KeMian1 1 state Key laboratory for Agrobiotechnology, China Agricultural university, Beijing 100193, China 2 Blackmountain laboratory, CSIRO plant Industry, Canberra, Australia 3 College of Animal Science and Technology, China Agricultural university, Beijing 100193, China state Key laboratory for Agrobiotechnology China Agricultural University Beijing China Blackmountain laboratory CSIRO Plant Industry Canberra Australia College of Animal Science and Technology China Agricultural University Beijing China

Fatty acid desaturase-2 (FAD2) introduces a double bond in position 12 in oleic acid (18:1) to form linoleic acid (18:2 n-6) in higher plants and microbes. A new transgenic expression cassette, containing CMV promoter/fad2 cDNA/SV40 polyA, was constructedto produce transgenic mice. Among 63 healthy offspring, 10 founders (15.9%) integrated the cotton fad2 transgene into their genomes, as demonstrated by PCR and Southern blotting analysis. All founder mice were fertile and heterozygous fad2 female and nontransgenic littermates were used for fatty acid analysis using gas chromatography. One fad2 transgenic line showed substantial differences in the fatty acid profiles and the level of linoleic acid was increased 19% (Pplant fad2 gene can be functionally expressed in transgenic mice and may playan active role in conversion of oleic acid into linoleic acid.

关键词： fatty acid desaturase FAD2 transgenic mouse oleic acid linoleic acid arachidonic acid

Mining subsidence control by solid backfilling under buildings

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中国有色金属学会会刊：英文版 2011年第S3期21卷 670-674页

作者： ZHA Jian-feng1, 2, 3, GUO Guang-li1, 2, FENG Wen-kai3, WANG Qiang1, 2 1. Key laboratory for Land Environment and Disaster Monitoring of the state Bureau of Surveying and Mapping, China university of Mining and Technology, Xuzhou 221116, China 2. Jiangsu Key laboratory of Resources and Environmental Information Engineering, China university of Mining and Technology, Xuzhou 221116, China 3. state Key laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu university of Technology, Chengdu 610059, China Key laboratory for Land Environment and Disaster Monitoring of the state Bureau of Surveying and Mapping China University of Mining and Technology Xuzhou 221116 China Jiangsu Key laboratory of Resources and Environmental Information Engineering China University of Mining and Technology Xuzhou 221116 China state Key laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Chengdu 610059 China

Solid backfilling mining can reduce the buildings' damage caused by mining greatly. The reduction of subsidence value, the slow advancing speed and the subsidence caused by backfilling body compaction are the main reasons that solid backfilling mining velocity decreases significantly. Based on the research of mechanism, some principles on subsidence control of solid backfilling mining under buildings were proposed. The equivalent mining height was designed according to the fortification criteria of buildings and their attachment structures, which enables the ground movement and deformation caused by mining to be less than the corresponding fortification criteria.

关键词： solid backfilling mining subsidence control mining under buildings

Mid-low temperature metallogenic province of South China: A continental-scale mineralization and temperature zoning

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Chinese Science Bulletin 1999年第S2期44卷 27-29页

作者： MA Dongsheng state Key laboratory for research of Mineral Deposits, Nanjing university Nanjing 210093, China state Key laboratory for research of Mineral Deposits Nanjing University Nanjing 210093 China

CHARACTERIZED by Au,Sb and Hg mineralization, the mid-low temperature metallogenic province in SouthChina is composed of the Au-Sb belt in the Proterozoic group on the Jiangnan Old Land, and the Hunan-Guizhou Hg belt in the Cambrian system, the SW China Carlin-type Au belt in the Permian andTriassic, and the Mid-Hunan Sb belt in the Devonian. The latter three are located in the basins respectively on the NW,SW and SE of the Jiangnan Old Land. The province holds nearly 50% Sb ore reserves and supplies about 70% annual antimony mine production in the world, and about 80% both ofexplored reserves and annual output of Hg in China. In the last twenty years, discovery of the micrograined dissemination Au deposits in the SW China Au belt made an important breakthrough in

关键词： South China continental-scale mineralization ore-forming temperature zoning.

Sediment pollution and its effect on fish through food chain in the Yangtze River

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International Journal of Sediment research 2008年第4期23卷 338-347页

作者： Yujun YI 1,Zhaoyin WANG 2,Kang ZHANG 3,Guoan YU 4,and Xuehua DUAN 51 Dr.,Lecturer,state Key laboratory of Hydroscience,School of Environment,Beijing Normal university,Beijing 100875,China2 Prof.,state Key laboratory of Hydroscience and Engineering,Tsinghua university,Beijing 100084,China 3 Ph.D.,state Key laboratory of Hydroscience and Engineering,Tsinghua university,Beijing 100084,China 4 Ph.D.,state Key laboratory of Hydroscience and Engineering,Tsinghua university,Beijing 100084,China 5 Ph.D.,state Key laboratory of Hydroscience and Engineering,Tsinghua university,Beijing,100084,China Dr. Lecturer State Key Laboratory of Hydroscience School of Environment Beijing Normal University Beijing 100875 China Prof. State Key Laboratory of Hydroscience and Engineering Tsinghua University Beijing 100084 China Ph. D. State Key Laboratory of Hydroscience and Engineering Tsinghua University Beijing 100084 China Ph. D. State Key Laboratory of Hydroscience and Engineering Tsinghua University Beijing 100084 China

Suspended sediment adsorbs pollutants from flowing water in rivers and deposits onto the bed. However, the pollutants accumulated in the river bed sediment may affect the bio-community through food chain for a long period of time. To study the problem the concentration of heavy metals (Cr, Cd, Hg, Cu, Fe, Zn, Pb and As) in water, sediment, and fish/invertebrate were investigated in the middle and lower reaches of the Yangtze River during 2006-2007. The concentrations of heavy metals were 100-10,000 times higher in the sediment than in the water. Benthic invertebrates had relatively high concentrations of heavy metals in their tissues due to their proximity to contaminated sediments. Benthic invertivore fish had moderately high concentrations of heavy metals whereas phytoplanktivore fish, such as the silver carp, accumulated the lowest concentration of heavy metals. The concentrations of Cu, Zn, and Fe were higher than Hg, Pb, Cd, Cr, and As in the tissue samples. The concentration of heavy metals was lower in the river sediments than in the lake sediments. Conversely, the concentration of heavy metals was higher in river water than in lake water. While a pollution event into a water body is often transitory, the effects of the pollutants may be long-lived due to their tendency to be absorbed in the sediments and then released into the food chain. The heavy metals were concentrated in the following order: bottom material > demersal fish and benthic fauna > middle-lower layer fish > upper-middle layer fish > water.

关键词： Sediment pollution Heavy metal Fish Invertebrate Yangtze River

Differential Hydrological Grey Model (DHGM) with self-memory function and its application to flood forecasting

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Science China(Technological Sciences) 2009年第4期52卷 1039-1049页

作者： CHEN XiangDong1, XIA Jun1,2 & XU Qian3 1 Key laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources research, Chinese Academy of Sciences, Beijing 100101, China 2 state Key laboratory of Water Resources and Hydropower Engineering Science, Wuhan university, Wuhan 430072, China 3 School of Hydrology and Water Resources, Hohai university, Nanjing 210098, China Key laboratory of Water Cycle and Related Land Surface Processes Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China state Key laboratory of Water Resources and Hydropower Engineering Science Wuhan University Wuhan China School of Hydrology and Water Resources Hohai University Nanjing China

This paper addresses a problem of flood forecasting with the self-memory function. Considering flood forecasting’s uncertainty and updating demand, a hybrid hydrological model, namely Differential Hy- drological Grey Model with self-memory function (DHGM-SM), is developed. The model has two fold features. One is to establish a self-memorization equation linked with DHGM, that could extract useful information from past data series and realize updating of hydrological dynamic process. The other is that this model has higher efficiency relative to original hydrological model without self-memory func- tion. This approach was applied to river flow forecasting of two representative basins in Tunxi of South China and Daqinggou of North China. It is shown that this hybrid method has satisfactory forecasting accuracy by examination of both calibration and validation.

关键词： differential grey model the self-memorization principle real-time forecasting faded-memory recursive least square method

Ubiquitination-mediated protein degradation and modification:an emerging theme in plant-microbe interactions

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Cell research 2006年第5期16卷 413-426页

作者： Li-Rong Zeng~1 Miguel E Vega-Sánchez~1 Tong Zhu~2 Guo-Liang Wang~(1,3)~1Department of plant Pathology and plant Molecular Biology and Biotechnology Program,The Ohio state university,Columbus,OH 43210,USA:~2Syngenta Biotechnology Inc.research Triangle Park,NC 27709-2257.USA:~3Rice Genomics laboratory,Human Agricultural university,Changsha,Human 410128,China Department of plant Pathology and plant Molecular Biology and Biotechnology Program The Ohio State University Columbus USA Syngenta Biotechnology Inc Research Triangle Park USA Rice Genomics laboratory Hunan Agricultural University Changsha China

Post-translational modification is central to protein stability and to the naodulation of protein *** types ofprotein modification,such as phosphorylation,methylation,acetylation,myristoylation,glycosylation,and ubiquitina-tion,have been *** them,ubiquitination distinguishes itself from others in that most of the ubiquitinatedproteins are targeted to the 26S proteasome for *** ubiquitin/26S proteasome system constitutes the majorprotein degradation pathway in the *** recent years,the importance of the ubiquitination machinery in the controlof numerous eukaryotic cellular functions has been increasingly *** number of E3 ubiquitin ligasesand their substrates,including a variety of essential cellular regulators have been *** in the past severalyears have revealed that the ubiquitination system is important for a broad range of plant developmental processes andresponses to abiotic and biotic *** review discusses recent advances in the functional analysis of ubiquitina-tion-associated proteins from plants and pathogens that play important roles in plant-microbe interactions.

关键词： Ubiquitination defense response plant-microbe interactions U-box protein Sp111