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TESTS AND APPLICATIONS OF A BOUSSINESQ MODEL WITH AMBIENT CURRENT

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Journal of Hydrodynamics 2010年第4期22卷 526-536页

作者： ZHANG Hong-sheng WANG Wei-yuan FENG Wen-jing WANG Ya-ling YANG Jian-min State Key Laboratory of ocean Engineering Shanghai Jiao Tong University Shanghai 200240 China shanghai Water Planning and Design Research Institute Shanghai 200232 China college of Transport and Communications Shanghai Maritime University Shanghai 200135 China

A new type Boussinesq model is proposed and applied for wave propagation in a wave flume of uniform depth and over a submerged bar with current present or absent,***,for the propagation of monochromatic incident wave with current absent,the Boussinesq model is tested in its complete form,and in a form without the introduction of utility velocity *** is validated that the introduction of utility velocity variables can improve the characteristics of velocity field,dispersion and *** versions of the Boussinesq models are of higher accuracy than the fully-nonlinear fourth-order model,which is one of the best forms among the existing traditional Boussinesq models that do not incorporate breaking mechanism in one ***,the Boussinesq model in its complete form is applied to simulating the propagation of bichromatic incident waves with current present or absent,respectively,and the modeled results are compared to the analytical ones or the experimental *** modeled results are reasonable in the case of inputting bichromatic incident waves with the strong opposing current present.

关键词： Boussinesq model ambient current wave-wave interactions velocity field bichromatic incident waves

A new LC-MS method for efficiently detecting Diarrhetic shellfish poisoning toxins

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A new LC-MS method for efficiently detecting Diarrhetic shel...

Cryopreservation of amphioxus (Branchiostoma belcheri) spermatozoa

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： RAO Tao~1,XU Ren~2,,TANG Chen~3,DU Xia~3 JIA Rui~3,Le-qing Hu~3,Pei-min He~(3*) (1.college of food science& technology,shanghai ocean university,shanghai 201306 2.East China Sea Environmental Monitoring Center,State oceanic Administration, shanghai 200137 3.college of Fisheries and Life science,shanghai ocean university, shanghai 201306)

An exploratory study on the detection of Diarrhetic Shellfish Poisoning(DSP) toxins,Okadaic Acid(OA) and Dinophysistoxins-1(DTX-1),was performed by using Ultra Performance Liquid Chromatography(UPLC) coupled with Quadrupole Time-Of-Flight Mass Spectrometry(Q-TOFMS).Extraction from shellfmh tissue was achieved with 80%aqueous *** separation was performed on a UPLC Acquity BEH column(2.1 mm×1OOmm,1.7um) with methanol water(85:15,v / v) containing 0.5 mmol / L ammonium acetate as eluent at a flow rate of 0.40 m L / *** protonated OA and DTX-l molecule were selected as a precursor ion scanning for Q-TOFMS in the negative electrospray ionizaion *** results indicate that UPLC /Q-TOFMS is suitable for qualitative and quantitative analysis of OA and DTX-l,the anverage recoveries of OA and DTX-1 spiked to tissue homogenates through the complete cleanup procedure ranged from 97.5%to 100.2%,and has high accuracy(RSDfood safety determination and red tide monitoring *** results of the detection of the muscle sample from the Meretrix lusoria fed with Prorocentrum lima show that the toxin concentration in the shellfish muscle increases over feeding *** OA and DTX-l toxins were detected in the samples of 6 species of shellfish from shanghai market.

关键词： ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry Diarrhetic shellfish poisoning(DSP) okadaic acid(OA) dinophysistoxin-1(DTX-1)

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Acta oceanologica Sinica 2009年第2期28卷 96-102页

作者： XU Yuyan ZHANG Shicui VERAPONG Vuthiphandchai SUBUNTITH Nimrat college of food science and Engineering Ocean University of China Qingdao 266003 China college of Marine Biology Ocean University of China Qingdao 266003 China Department of Aquatic science Burapha University Chonburi 20131 Thailand Department of Microbiology and Environmental science Program Faculty of Science Burapha University Chonburi 20131 Thailand

Amphioxus, Branchiostoma belcheri, a closest relative of vertebrates, is at a high risk of extinction due to a combination of low effective population size, altered native habitats and environmental pollution, yet little is known about cryopreservation of its gametes. This study deals with the cryopreservation of amphioxus senlen. The main findings are that （1） the extender of Yao et al. is the best one among the four extenders examined; （2） the appropriate ratio of semen to extender of Yao et al. plus cryoprotectant is from 1：5 to 1：7; （3） dimethyl sulfoxide （DMSO） and methanol are the better cryoprotectants than glycerol, with DMSO giving the best results; （4） the eggs fertilized with post-thaw spermatozoa are capable of developing to at least hatching stage, and the highest hatching rate is （12.4±3.0）%. This is the first report on freezing and thawing of amphioxus spermatozoa, providing a simple and practical protocol for cryopreservation of amphioxus spermatozoa and laying a foundation for safeguarding this endangered species.

关键词： amphioxus Branehiostoma beleheri cryopreservation spermatozoa motility

Study on roughness coefficient for unsubmerged reed in the Changjiang Estuary

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Acta oceanologica Sinica 2011年第5期30卷 108-113页

作者： GU Fengfeng MU Lin QI Dingman LI Junhua KONG Lingshuang WAN Yuanyang shanghai Estuary and Coastal science Research Center Shanghai 201201 China National Marine Data and Information Service State Oceanic Administration Tianjin 300171 China college of ocean Environment and Engineering Shanghai Maritime University Shanghai 200135 China

The influence of density,foliage and stem flexibility on the roughness coefficients under unsubmerged conditions,such as Manning＇s n,is investigated *** instrumentation system has been developed for measuring the flow rate ranging from 0.1 to 0.3 L/s under the condition of different artificial foliated *** on the experimental results,the influence on the relationship between n with different density,foliage,flexibility and flow depth is *** is found that the foliage and the density are the important factors affecting Manning＇s *** a range of relatively low velocity and relatively large bending stiffness of stem,Manning＇s n is not influenced significanthy by the flexibility of stem.

关键词： Manning's coefficient foliage density flexibility reed

EST-derived microsatellites in P seudosciaena crocea and their applicability to related species

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Acta oceanologica Sinica 2010年第6期29卷 83-91页

作者： YE Hua WANG Xiaoqing GAO Tianxiang WANG Zhiyong college of Animal science and technology Hunan Agricultural University Changsha 410128 China Key Laboratory of science and technology for Aquaculture and food Safety Fisheries College/Fisheries Biotechnology Institute Jimei University Xiamen 361021 China Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 China

Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI *** for 48 ESTSSR loci were designed and screened with 30 *** specimens captured from Guanjingyang sea area in Fujian Province of *** of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of *** observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,*** information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on ***,***,***,*** and *** revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these ***,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.

关键词： Pseudosciaena crocea EST-SSRs genetic diversity cross-species amplification

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3种淡水对虾在冻藏过程中蛋白质特性的变化

水产学报 2010年第3期34卷 389-394页

作者：陶欢陈舜胜上海海洋大学食品学院

以目前市场上主要的3种养殖对虾为对象,以肌原纤维蛋白盐溶解度、Ca2+-ATPase活性与蛋白质表面的巯基含量为指标,探讨了不同温度冻藏过程(-10、-20、-30、-40℃)对虾虾肉蛋白质生物化学特性的变化,评价了不同冻藏温度对对虾品质的影响... 详细信息

以目前市场上主要的3种养殖对虾为对象,以肌原纤维蛋白盐溶解度、Ca2+-ATPase活性与蛋白质表面的巯基含量为指标,探讨了不同温度冻藏过程(-10、-20、-30、-40℃)对虾虾肉蛋白质生物化学特性的变化,评价了不同冻藏温度对对虾品质的影响。结果表明,第一,3种蛋白质的冷冻变性指标应用于对虾时出现一定差异。Ca2+-ATPase活性比较灵敏,能较好反映-10～-40℃不同冻藏温度引起的蛋白质变性的程度。肌原纤维蛋白盐溶解度能够反映-20℃以上冻藏过程中蛋白质变性程度的差异,而-20～-40℃的差异难以区分。-10℃冻藏时,肌原纤维蛋白的盐溶解度下降明显,而-20℃冻藏时下降程度较小,-20、-30、-40℃冻藏时肌原纤维蛋白的盐溶解度没有明显区别。-10～-40℃冻藏对于巯基含量变化都不大。第二,冻藏过程中3种对虾蛋白质冷冻变性较轻度,比一般鱼类在冻藏过程中稳定,虾比大部分鱼类更耐冻。第三,冻藏温度越低Ca2+-ATPase活性下降程度越小,-10～-40℃冻藏时其差异性有比较明显区分,表明Ca2+-ATPase活性更适合于虾的质量评价。第四,-20℃冻藏对于虾类是比较经济合理的,实用冻藏温度可取-20℃。

关键词：淡水对虾冻藏肌原纤维蛋白冷冻变性

Sequence Stratigraphic Analysis of the First Layer,Upper Second Submember,Shahejie Formation in Pucheng Oilfield

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Journal of Earth science 2009年第6期20卷 932-940页

作者：张金亮江志强李德勇孙婧 college of Resources science and technology Beijing Normal University college of Marine Geosciences Ocean University of China China National Offshore Oil Corporation

In view of the high accuracy and predictability, high-resolution sequence stratigraphy had been extensively applied to oil exploration and gotten prominent practicable results. This article takes the first layer, upper second submember, Shahejie （沙河街） Formation from Pucheng （濮城） oilfield as an example to analyze the application of high-resolution sequence stratigraphy in reservoir study on the basis of a comprehensive study of core log data. Firstly, facies analysis of this area reveals the corresponding terminal fan system occurring where sediment-laden streams decrease in size and vanish as a result of evaporation and transmission losses. The model includes a tripartite zonation of terminal fan into feeder, distributary, and basinal zones. Secondly, electrofacies were made by well-log analysis and then matched with sedimentary facies defined by core analysis. Four electrofacies characterizing the main sedimentary facies association and depositional environments within target area are defined （channel, lag deposit, lake or flood-plain, and overflow deposits）. Thirdly, related correlations based on high-resolution sequence stratigraphy were established. By observing the stacking arrangement of genetic sequences, different scales of stratigraphic cycle can be identified. Within scale and duration, the stratigraphic cycles are termed as genetic sequences, genetic sequence sets, and minor cycles.

关键词： terminal fan high-resolution sequence stratigraphy electrofacies base level.

Fuzzy Probabilistic Analysis of Seismic Stability of Coastal Embankment

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China ocean Engineering 1996年第1期11卷 99-105页

作者： He Guangne Yang Bin Wen Nai Professor, Dalian university of technology, Dalian 116023 Engineer, Design and Research Institute of shanghai Harbour Engineering, shanghai 200032 . Lecturer, Dalian Fisheries college, Dalian 116023 Dalian university of technology Dalian 116023 Design and Research Institute of shanghai Harbour Engineering Shanghai 200032 Dalian Fisheries college Dalian 116023

The stability of slope is affected by a number of factors, some of which have not only random property but also fuzzy characteristic. Therefore, the analysis of slope stability is really an uncertain problem. The customary safety factor does not in reality reflect stability scientifically, quantitatively and practically. In order to obtain more practical results, the slope stability is treated as a fuzzy random event for the evaluation of its fuzzy probability. Finally, the seismic stability of an existing coastal embankment is analyzed by means of the suggested fuzzy probabilistic method. It may be seen that the results of analysis can more fully represent the numerical assessment of the degree of slope seismic stability.

关键词： coastal embankment seismic stability fuzzy probabilistic analysis

顶空固相微萃取-气相色谱-质谱-嗅觉测量联用初探鲢肉的挥发性风味物质

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水产学报 2009年第4期33卷 692-696页

作者：张青王锡昌刘源上海海洋大学食品学院

为探明鲢肉挥发性气味特征物质,通过顶空固相微萃取法提取和浓缩了鲢肉中的挥发性物质,并利用气相色谱-质谱联用以及气相色谱-嗅觉测量法对气味物质进行了定性分析及气味特征的评价。由8人组成的评价小组通过检测频率法进行嗅觉测量。... 详细信息

为探明鲢肉挥发性气味特征物质,通过顶空固相微萃取法提取和浓缩了鲢肉中的挥发性物质,并利用气相色谱-质谱联用以及气相色谱-嗅觉测量法对气味物质进行了定性分析及气味特征的评价。由8人组成的评价小组通过检测频率法进行嗅觉测量。结果表明,经GC-MS分析鉴定得到了29种挥发性气味物质,主要是一些羰基类和醇类化合物,占了总挥发性物质的88%。通过检测频率分析法鉴定出12种化合物具有气味特征,表现为鱼腥、金属、青草、油脂氧化、蘑菇等特征。另外有4种未知物也显示了较强的风味特性,它们协同作用构成了鲢的特殊气味。

关键词：鲢固相微萃取气质联用嗅觉测量