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Overexpression of p27^(KIP1)induced cell cycle arrest in G_1 phase and subsequent apoptosis in HCC-9204 cell line

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World Journal of Gastroenterology 2000年第4期6卷 513-521页

作者： Jiang Li Xin Ke Yang Xin Xin Yu Meng Liang Ge Wen Liang Wang Jie Zhang Yun De Hou Department of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,china State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100052,china Department of dermatology,Beijing Hospital,Beijing 100016,china institute of Radiation Medicine,Beijing 100085,china 第四军医大学陕西西安中国预防医学科学院病毒所北京中国预防医学科学院病毒所分子病毒基因工程国家重点实验室北京北京医院北京解放军军事医学科学院放射医学所北京

AIM We have previously reported that inducible over-expresaion of Bak may prolong cell cycle in G1 phase and lead to apoptosis in HCC-9204 cells. This study is to investigate whether p27KIP1 plays an important role in this process. MEHODS In order to elucidate the exact function of p27KIP1 in this process, a zinc inducible p27KIP1 stable transfectant and transient p27KIP1- GFP fusion transfectant were constructed. The effects of inducible p27KIP1 on cell growth, cell cycle arrest and apoptosis were examined in the mock, control pMD vector, and pMD-KIP1 transfected HCC-9204 cells. RESULTS This p27KIP1-GFP transfectant may transiently express the fusion gene. The cell growth was reduced by 35% at 48 h of p27KIP1 induction with zinc treatment as determined by trypan blue exclusion assay. These differences remained the same after 72 h of p27KIP1 expression, p27KIP1 caused cell cycle arrest after 24 h of induction, with 40% increase in G1 population. Prolonged p27KIP1 expression in this cell line induced apoptotic cell death reflected by TUNEL assay. Fourty-eight h and 72 h of p27KIP1 expression showed a characteristic DNA ladder on agarose gel electrophoresis.

关键词： p27^(KIP1) apoptosis cell cycle inducible expression system carcinoma hepatocellular liver neoplasms

Chinese Expert Consensus on the Diagnosis and Treatment of Adult Dermatomyositis (2022)

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International Journal of dermatology and Venereology 2024年第3期7卷 163-173页

作者： Hua Cao Ai-Jun Chen Yong Cui Dan-Qi Deng Xing-Hua Gao Yan-Ling He Xiao-Jing Kang Hong-Zhong Jin Cheng-Xin Li Feng Li Heng-Jin Li Wen-Jun Liao Xiao-Ming Liu Qian-Jin Lu Yan Lu Meng Pan Wei-Hua Pan Xiao-Ming Shu Qing Sun Ke-Yun Tang Juan Tao Yu Wang Ting Xiao Fu-Ren Zhang Han-Lin Zhang dermatology Branch of the china International Exchange and Promotion Association for Medical and Health Care National Clinical Research Center for Dermatologic and Immunologic Diseases Department of dermatology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of dermatology First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of dermatology China-Japan Friendship HospitalBeijing 100029China Department of dermatology the Second Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650101China Department of dermatology the First Hospital of China Medical UniversityShenyangLiaoning 110001China Department of dermatology Beijing Chaoyang Hospital Affiliated to Capital Medical UniversityBeijing 100020China Department of dermatology and Venereology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqiXinjiang Uygur Autonomous Region 830001China Department of dermatology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China Department of dermatology First Medical CenterPLA General HospitalBeijing 100853China Department of dermatology Hainan Hospital of PLA General HospitalSanyaHainan 572013China Department of dermatology Xijing HospitalAir Force Medical UniversityXi’anShaanxi 710032China Department of dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenGuangdong 518052China Hospital for Skin Diseases(institute of dermatology) Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of dermatology Jiangsu Province Hospitalthe First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of dermatology Shanghai Changzheng HospitalSecond Affiliated Hospital of Naval Medical UniversityShanghai 200003China Department of Rheumatology China-Japan Friendship HospitalBeijing 100029China Department of dermatology Qilu Hospital of Shandong UniversityJinanShandong 250012China Department of dermatology Union Hospital

Dermatomyositis,an idiopathic inflammatory myopathy,is characterized by distinctive skin manifestations,proximal muscle weakness,and multiple organ involvement and can be accompanied by *** provide a reference for dermatologists and clinicians in other relevant fields of clinical practice,experts from the dermatology Branch of the china International Exchange and Promotion Association for Medical and Health Care and the National Clinical Research Center for Dermatologic and Immunologic Diseases developed this consensus on the diagnosis and treatment of adult dermatomyositis using Chinese and international literature and expert advice.

关键词： dermatomyositis adult diagnosis treatment clinical protocols expert consensus

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Eosinophilic pustular folliculitis:case report and clinical analysis

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Eosinophilic pustular folliculitis:case report and clinical ...

The Second Eastern Asia dermatology Congress(EADC2012)

作者： GAO Min Department of dermatology at No.1 Hospital& institute of dermatology Anhui

＜正＞Background Eosinophilic pustular folliculitis（EPF） is a rare dermatosis characterized by recurrent outbreaks of papulopustular skin lesions mainly distributed in seborrheic *** eruptions often are associated with peripheral blood eosinophilia and occur mainly on the face,upper back and upper *** are 3 variants:classic EPF （Ofuji disease）,immunosuppression-associated EPF,and infancy-associated *** to now,there is no study to summary the characters for the Chinese EPF *** study aimed to understand the clinical features and pathogenesis of EPF in Chinese patients. Methods We reported an EPF patient and performed the clinical analysis for all the patients published in Chinese journals in recent ten years. Results There are 19 males and 1 female in these *** is a rare dermatosis characterized by recurrent outbreaks of papulopustular skin lesions mainly distributed in seborrheic areas（mainly on the face,upper back,and upper extremities） as annular *** could be associated with *** found that peripheral blood eosinophils increasing in 15 cases and non-increasing in 5 cases. Conclusions The male incidence of EPF is higher than in *** are different clinical features in different *** could be or not accompanied with the increasing of peripheral blood eosinophils.

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Association analyses identify six new psoriasis susceptibility loci in the Chinese population

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Association analyses identify six new psoriasis susceptibili...

中华医学会第十八次全国皮肤性病学术年会

作者： Xuejun Zhang institute of dermatology and Department of dermatology at No.l Hospital,Anhui Medical University

We extended our previous genome-wide association study for psoriasis with a multistage replication study including 8,312 individuals with psoriasis(cases) and 12,919 controls from china as well as 3,293 cases and 4,188 controls from Germany and the United States and 254 nuclear families from the United *** identified six new susceptibility loci associated with psoriasis in the Chinese study containing the candidate

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猪胸腺素(F5)治疗麻风的初步报告

中国医学科学院学报 1981年第3期3卷 198-200页

作者： institute of dermatology, Chinese Academy of Medical Sciences Qinhu Leprosarium, Tai County 中国医学科学院皮肤病研究所泰县溱湖医院

机体抗御麻风杆菌的能力主要依赖T淋巴细胞参与的细胞免疫作用,而麻风病患者的免疫缺陷主要表现为T淋巴细胞量和质上的缺陷.因此,已有人在动物实验麻风感染和人麻风病患者身上作了胸腺移植的尝试,但胸腺中的提取物——胸腺素,在麻风临... 详细信息

机体抗御麻风杆菌的能力主要依赖T淋巴细胞参与的细胞免疫作用,而麻风病患者的免疫缺陷主要表现为T淋巴细胞量和质上的缺陷.因此,已有人在动物实验麻风感染和人麻风病患者身上作了胸腺移植的尝试,但胸腺中的提取物——胸腺素,在麻风临床上的应用国外未见报道.我们用从猪胸腺中提取的猪胸腺素第五组分(简称ThF5)治疗6例麻风病患者,取得一定效果.

关键词：麻风皮肤杆菌病胸腺素胸腺浸膏胸腺激素 F5 麻风病学

A Retrospective Analysis of V-beam 595nm Pulsed Dye Laser Treatment of Infantile Hemangioma in Hand

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A Retrospective Analysis of V-beam 595nm Pulsed Dye Laser Tr...

中华医学会第十八次全国皮肤性病学术年会

作者： Chunjun Yang,Shengxiu Liu,Sen Yang institute of dermatology & Department of dermatology,the First Hospital,Anhui Medical University

Background and Objective 595nm pulsed dye laser has been proven to be effective in treating vascular lesions,especially in infantile hemangioma in past 10 years in *** there was different effect in different *** objective of this study was to assess the long-term outcomes and complications of 595nm pulsed dye laser in treatment of infantile hemangioma arisen in hand in Chinese patients retrospectively.

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Treatment of Port Wine Stains With Topical Photodynamic Therapy,Using Pulsed Dye Laser as a Light Source,Compared With Pulsed Dye Laser Alone:A Randomized Clinic Trial

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Treatment of Port Wine Stains With Topical Photodynamic Ther...

中华医学会第十八次全国皮肤性病学术年会

作者： Shengxiu Liu,Chunjun Yang,Sen Yang institute of dermatology & Department of dermatology,the First Hospital,Anhui Medical University

Objectives The role of pulsed dye lasers(PDL) in the treatment of port wine stains(PWS) is not satisfactory in all *** aim was to compare PDL with local ALA and irradiated by PDL in the treatment of PWS. Methods We did a prospective,randomized controlled trial in which we enrolled 236 patients with PWS. We assigned patients to PDL treatment(PDL group,n=l 19) or local ALA and irradiated by PDL(PDT

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Successful treatment of dermatomyositis complicated by pyopneumothorax,tracheoesophageal fistula and chylothorax

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Successful treatment of dermatomyositis complicated by pyopn...

中华医学会第16次全国皮肤性病学术年会

作者： Yang Chunjun※,MD,Liu Shengxiu※,MD,Yang Sen※,MD,Zhang Xuejun※,MD. institute of dermatology & Department of dermatology at No.1 Hospital,Anhui Medical University,Hefei, PR china

Dermatomyositis complicated by spontaneous tracheoesophageal fistula,or mediastinal emphysema, or pneumothorax,occurs infrequently and may be difficult to successful treatment.A case is reported in which spontaneous tracheoesophageal fistula,chylothorax,pyopneumothorax and subcutaneous emphysema complicating dermatomyositis was successfully managed with artificial ventilation,closed drainage of thoracic cavity,nutrition by gullet,high dosage of intravenous immunoglobulin and

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SEMI-SYNTHESIS OF TRIPTONIDE

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Chinese Chemical Letters 1994年第7期5卷 551-552页

作者： Peng Cheng MA and Wei YAN(Department of Materia Medica, institute of dermatology, Chinese Academy of Medical Sciences and Peking Union Medical Collage. Nanjing 210042)

A *** showed and antifertil has been semi-synthesized from triptolide in 97% yield with chromic acid in one step.

关键词： SEMI-SYNTHESIS OF TRIPTONIDE

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