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Functional analysis of schistosomes EF-hand domain-containing tegument proteins

chinese Science Bulletin 2007年第15期52卷 2100-2107页

作者： YU FuDong1,2,3.KANG Bin1,3.LI YuanYuan3.4 & LI YiXue1,3.1 Bioinformatics center,Key Laboratory of Systems Biology,shanghai Institutes for Biological sciences,{3. academy of Sci-ences,shanghai 2.003.,china 2 Graduate University of chinese academy of sciences,shanghai 2.003.,china 3.shanghai center for Bioinformation Technology,shanghai 20023.,china 4 Key Laboratory of Systems Biology,shanghai Institutes for Biological sciences,{3. academy of sciences,shanghai 2.003.,china Bioinformatics center Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate University of chinese academy of sciences Shanghai China shanghai center for Bioinformation Technology Shanghai China Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Schistosomes cause schistosomiasis disease which severely threatens human health. Little is known about the functions of EF-hand domain containing schistosomes tegument proteins other than as an-tigens. More possible functions of these tegument proteins were investigated with in silico analyses including protein-protein functional interaction,site-specific variation and glycosylation modification. The analysis results suggested that schistosomes could actively modulate host immune responses for its own favor through functional interactions with host proteins with immunomodulatory function,and passively regulate host immune responses through sequence variation under positive selection and glycosylating the recognition sites of host immune attack. In addition,the analysis of the C-terminal domain of these tegument proteins indicated that they could assist schistosomes in escaping host immune attacks through inhibiting chemotaxis and non-complement fixing antibody (IgG4) responses. In summary,our results suggested that these tegument antigen proteins could assist schistosomes in escaping and modulating host immune responses for self-protection during the process of host-para-site interaction.

关键词：寄生虫血吸虫皮蛋白质健康

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Establishment of environmental specimen bank in shanghai

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Journal of Environmental sciences 2000年第4期12卷 398-401页

作者： ZHANG Yuan xun, WANG Yin song, QIAN Yin , TAN Ming guang, ZHI Ming, LI De yi, CHENG Feng, ZNANG Gui lin (shanghai Institute of Nuclear research, chinese academy of sciences, shanghai 201800, china) shanghai Institute of Nuclear research Chinese Academy of Sciences Shanghai 201800 China

The program of the shanghai Environmental Specimen Bank (SESB) has been established recently and its current status is briefly described. Typical specimen such as water and sediment of Suzhou creek, city particulate matter are collected and some results which have been obtained during the long term permanent operation of this project since 1998 are presented. Moreover, further aspects of environmental data evaluation and assessment are discussed.

关键词： specimen bank environment ecology

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Calculation of the Surface Segregation of Cu-0.3.at. pct Au Alloy

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Journal of Materials Science & Technology 2000年第6期16卷 639-640页

作者： Liping ZHENG and Risheng LI (International center for Material Physics, chinese academy of sciences, Shenyang 110015, china) Yuanxun ZHANG and Shi QIU ({3. Institute of Nuclear research, chinese academy of sciences, {3. 201800, china) Bingyao JI Int. Cent. for Material Phys. Chinese Acad. of Sci. Shenyang 110015 China

Monte Carlo simulation technique with the energetics described by the embedded atom method has been used to calculate the surface segregation of Cu-0.3.at. pct Au alloy at T=800 K. It is found that the component Au in... 详细信息

关键词： Cu Calculation of the Surface Segregation of Cu-0.3.at pct Au Alloy

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Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites

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Acta Biochimica et Biophysica Sinica 2005年第12期37卷 819-825页

作者： Ting-Qing GUO~(1,2) Jian-Yang WANG~(1,2) Sheng-Peng WANG~(1,2.3. Xiu-Yang GUO~(1,2) Ke-Wei HUANG3.3.Jun-Ting HUANG~3.Chang-De LU~11 Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological sciences.chinese {3. of sciences.shanghai 2.003..china 2 Graduate School of the chinese academy of sciences.shanghai 2.003..china 3.Serictdtu research Institute.chinese {3. of Agricultural sciences,Zhenjiang 212018.china Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Graduate School of the chinese academy of sciencesshanghai 2.003. China Sericultural research Institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posteriorsilk gland of silkworm and repressed in other *** binding sites of several transcription factorsrevolved in the silk gland transcription specificity of fibl promoter have been recognized,including SGFB,PSGF and *** we report the leak expression of the enhanced green fluorescent protein (EGFP)reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fiblpromoter with deletion of the 5′terminal 41 bp sequence,which is located at -650 nt to -610 nt upstream *** transcription starting *** of silk gland specificity of the promoters was performed by observationof green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinantAutographa californica multiple nuclear polyhedrosis virus carrying the EGFP reporter gene controlled bydifferent lengths of fibl *** results indicated that availability of the binding sites of several knownfactors,including SGFB,PSGF and BMFA,is not sufficient for intact silk gland transcription specificity offibl promoter,and there are possible inhibitor binding sites in the 41 bp sequence (-650 nt to -610 nt)upstream of the transcription starting site which may be required to repress the activity of fibl promoter inother tissues.

关键词： promoter specificity fibroin light chain silkworm Bombyx mori recombinant AcMNPV

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Progress in the detection of human genome structural variations

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Science china(Life sciences) 2009年第6期52卷 560-567页

作者： WU XueMei1,3.& XIAO HuaSheng1,2 1 center of Functional Genomics,Key Laboratory of System Biology,shanghai Institutes for Biological sciences,{3. academy of sciences,shanghai 2.003.,china 2 National Engineering center for Biochip at shanghai,shanghai 201203.china 3.Graduate School of the{13.,shanghai 2.003.,china center of Functional Genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of sciences Shanghai China National Engineering center for Biochip at shanghai Shanghai China

The emerging of high-throughput and high-resolution genomic technologies led to the detection of submicroscopic variants ranging from 1 kb to 3.Mb in the human *** variants include copy number variations(CNVs),inversions,insertions,deletions and other complex rearrangements of DNA *** paper briefly reviews the commonly used technologies to discover both genomic structural variants and their potential ***,we highlight the array-based,PCR-based and sequencing-based assays,including array-based comparative genomic hybridization(aCGH),representational oligonucleotide microarray analysis(ROMA),multiplex amplifiable probe hybridization(MAPH),multiplex ligation-dependent probe amplification(MLPA),paired-end mapping(PEM),and next-generation DNA sequencing ***,we discuss the limitations and challenges of current assays and give advices on how to make the database of genomic variations more reliable.

关键词： structural variation cytogenetic microarray PCR paired end mapping(PEM) next-generation sequencing

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Expression and purification of lipoprotein-associated phospholipase A2,a key enzyme involved in atherosclerosis

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Acta Pharmacologica Sinica 2006年第6期27卷 679-684页

作者： Fu-jun ZHANG~(2,4)Mao-jun CAI~3.Jing-kang SHEN~3.Yi-ping WANG~(2,5)2 State Key Laboratory of Drug research.3.Department of Medicinal Chemistry,shanghai Institute of Materia Medica,shanghai Institutes for Biological {3.,chinese academy of {3..shanghai 201203.china 4 Graduate School of the chinese academy of sciences.chinese academy of {3.,shanghai 201203.china State Key Laboratory of Drug research Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of sciences Chinese Academy of Sciences Shanghai China Department of Medicinal Chemistry Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To express and purify lipoprotein-associated phospholipase A2（Lp-PLA2）,and to establish a screening model for Lp-PLA2inhibitors using the expressed Lp-PLA2,Methods:We cloned the full-length cDNA of Lp-PLA2 from differentiatedTHP-I cells,and subcloned the cDNA into the baculovirus transfer *** addition,we introduced an N-terminal Kozak sequence for high-level translation initiation and a C-terminal sequence of 6 histidine residues forpurification,The fusion enzyme was expressed in Sf9 insect cells and purified byNi2+affinity *** Lp-PLA2activity was measured us-ing[3.]PAF as a substrate,and we examined the enzyme activity of recombinantLp-PLA2pretreated with the known Lp-PLA2inhibitor ***:Wesuccessfully cloned the full-length Lp-PLA2gene from differentiated THP-1 *** fusion enzyme was expressed in Sf9 insect cells at a high level and purifiedefficiently through a 2-step *** recombinant Lp-PLA2activity wasmeasured using[3.]PAF as a substrate,and proved to be enzymatically ***-PLA2inhibitor SB43.495 produced a good inhibition curve for inhibition ofrecombinant Lp-PLA2with an IC50of 57±1μmol/***:We expressed andpurified Lp-PLA2at a high level in insect cell-baculovirus expression *** ratio was much greater than that obtained from human plasma and we estab-lished a screening model for Lp-PLA2inhibitors using the expressed Lp-PLA2.

关键词： lipoprotein-associated phospholipase A2 atherosclerosis coronary heart diseases cloning baculovirus purification

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Conus Peptides-A Rich Pharmaceutical Treasure

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 713-723页

作者： Cheng-Zhong WANG and Cheng-Wu CHIInstitute of Biochemistry and Cell Biology, shanghai Institute of Biological sciences, the chinese academy of sciences,{3., china Institute of Neuroscience, the chinese academy of sciences,{3., china Institute of Protein research, Tongji University, shanghai 200092, china Institute of Biochemistry and Cell Biology Shanghai Institute of Biological Sciences the Chinese Academy of SciencesShanghai 200031 China Institute of Neuroscience the Chinese Academy of SciencesShanghai 200031 China Institute of Protein research Tongji UniversityShanghai 200092 China

Marine predatory cone snails (genus Conus) with over 500 species represent what is arguably the largest single genus of marine animals alive today. All Conus are venomous and utilize a complex mixture of Conus peptide... 详细信息

关键词： Conus peptide conotoxin neuropathic disorder pharmaceutical potency

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Recombinant scFv Antibodies against E Protein and N Protein of Severe Acute Respiratory Syndrome Virus

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 541-547页

作者： Hui LIU , Yan-Li DING, Wei HAN, Mei-Yun LIU, Rui-Yang TIAN, Sheng-Li YANG, and Yi GONGshanghai research center of Biotechnology, shanghai Institutes of Biological sciences, chinese academy of sciences,Graduate School of the chinese academy of sciences, shanghai 20023., china shanghai research center of Biotechnology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200233 China. huiliu@***

Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, Kd) of SARS-CoV E protein was 5.7×10–8 M for B10 and 8.9×10–8 M for C20. The affinity of A17 for N protein was 2.1×10–6 M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot.

关键词： SARS-CoV antibody scFv phage display affinity chromatography

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Development of a quantitative,cell-based,high-content screening assay for epidermal growth factor receptor modulators

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Acta Pharmacologica Sinica 2007年第10期28卷 1698-1704页

作者： Jue WANG~2,Xin XIE~(2,3.~2 The National center for Drug Screening,shanghai Institute of Materia Medica,chinese {3. of sciences Graduate School of chinese {3.of sciences shanghai 201203.china The National center for Drug Screening Shanghai Institute of Materia Medica Chinese Academy of Sciences Graduate School of chinese academy of sciences Shanghai China

Aim:To develop a robust,cell-based,high-content screening （HCS） assay basedon receptor internalization for the identification of novel modulators of the epi-dermal growth factor receptor （EGFR）.Methods:Agonist-induced receptorinternalization is part of the signaling cascade of ***-taggedepidermal growth factor （EGF） was used to visualize the internalized receptor-ligand *** fluorescent intracellular spots were detected and mea-sured with an ArrayScan HCS *** that can competitively bind toEGFR or interfere with EGFR internalization process would result in a reducednumber and intensity of intracellular fluorescent *** assay was validated,optimized,and applied to a large-scale screening of a library containing 48 000synthetic ***:The competition between fluorescent EGF andunlabeled EGF reveals the IC50of unlabeled EGF is approximately 0.2 nmol/L,which is comparable with other published *** compounds with arelatively high degree of interference with EGFR internalization were *** of the compounds was proven to be agonist of the EGFR since it inducedphosphorylation of the receptor and extracellular signal-regulated protein ki-nase （ERK）.Conclusion:This automated,objective,and easy-to-use assay pro-vided abundant information,quantitative results,and demonstrated the potentialuse of HCS methods in searching membrane receptor modulators.

关键词： high-content screening epidermal growth factor receptor receptor internalization competitive binding

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Integrated precise orbit determination of Shenzhou IV unmanned spacecraft

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Science china(Technological sciences) 2004年第5期47卷 518-525页

作者： L Congmin1,2, GU Yidong3. LIN Baojun3.& GUO Jiong3.1. center for Space Science and Applied research, chinese academy of sciences, Beijing 100080,{3. 2. Graduate School of the chinese academy of sciences, Beijing 10003.,{3. 3. General Establishment of Space Science and Application, chinese academy of sciences, Beijing 100080,{3. Correspondence should be addressed to L Congmin center for Space Science and Applied research Chinese Academy of Sciences Beijing China Graduate School of the chinese academy of sciences Beijing China General Establishment of Space Science and Application Chinese Academy of Sciences Beijing China

Considering the characteristic orbit of Shenzhou IV spacecraft and the advantages and disadvantages of several types of domestic orbit measurements, a scheme of IPOD is determined, which uses GPS, SLR and USB observation data jointly plus precise ionosphere corrections to GPS observation data. IPOD system and mathematic models of OD are introduced. The results of different joint OD are analyzed using true observation data, and show that radial accuracy is better than 2 m.

关键词： Shenzhou IV unmanned spacecraft integrated precise orbit determination GPS SLR USB.

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