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Characterization of a novel developmentally retarded mutant (drm1) associated with the autonomous flowering pathway in Arabidopsis

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Cell Research 2005年第2期15卷 133-140页

作者： Yong ZHU1,2,*, Hui Fang ZHAO1,3.*, Guo Dong REN1, Xiao Fei YU1, Shu Qing CAO1, Ben Ke KUAI1,4,**1Department of Biochemistry, Ministry of Education Key laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 20043., china 2School of Horticulture and Gardening, southwest forestry {3., Kun Ming 6502.4, china 3.epartment of biology, School of Life and Environment Science, Shanghai Normal University, Shanghai 20023., china4Fudan Centre for Genetic Diversity and designed Agriculture, Fudan University, 220 Handan Road, Shanghai 20043., china Department of Biochemistry Ministry of Education Key laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University 220 Handan Road Shanghai 200433 China.

A developmentally retarded mutant (drm1) was identified from ethyl methanesulfonate (EMS)-mutagenized M2 seedsin columbia (col-0) genetic background. The drm1 flowers 109 d after sowing, with a whole life cycle of about 160 *** also shows a pleiotropic phenotype, e.g., slow germination and lower germination rate, lower growth rate, curlingleaves and abnormal floral organs. The drm1 mutation was a single recessive nuclear mutation, which was mapped tothe bottom of chromosome 5 and located within a region of 20-3. kb around MXK3.1. There have been no mutantswith similar phenotypes reported in the literature, suggesting that DRM1 is a novel flowering promoting locus. Thefindings that the drm1 flowered lately under all photoperiod conditions and its late flowering phenotype was significantlyrestored by vernalization treatment suggest that the drm1 is a typical late flowering mutant and most likely associatedwith the autonomous flowering pathway. The conclusion was further confirmed by the revelation that the transcriptlevel of FLC was constantly upregulated in the drm1 at all the developmental phases examined, except for a very earlystage. Moreover, the transcript levels of two other important repressors, EMF and TFL1, were also upregulated in thedrm1, implying that the two repressors, along with FLC, seems to act in parallel pathways in the drm1 to regulateflowering as well as other aspects of floral development in a negatively additive way. This helps to explain why the drm1exhibits a much more severe late-flowering phenotype than most late-flowering mutants reported. It also implies that theDRM1 might act upstream of these repressors.

关键词： drm1 late-flowering autonomous flowering pathway Arabidopsis thaliana.

Identification, characteristic and phylogenetic analysis of type II DNA topoisomerase gene in Giardia lamblia

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Cell Research 2005年第6期15卷 474-482页

作者： De HE1, 2, 3. Jian Fan WEN1,*, Wan Qun CHEN1, Si Qi LU4, De Dong XIN1, 21 Key Laboratory of Cellular and Molecular Evolution, kunming Institute of Zoology, Chinese Academy of Sciences, kunming 650223. china2 Gradulate School of the Chinese Academy of Sciences, Beijing 10003., china3.southwest forestry co.lege, kunming 6502.4, china4 Capital University of Medical Sciences, Beijing 100054, china KeyLaboratoryofCellularandMolecularEvolution KunmingInstituteofZoologyChineseAcademyofSciencesKunming650223China

The genes encoding type II DNA topoisomerases were investigated in Giardia lamblia genome, and a type IIA gene,GlTop 2 was identified. It is a single copy gene with a 4476 bp long ORF without intron. The deduced amino acidsequence shows strong homology to eukaryotic DNA Top 2. However, some distortions were found, such as sixinsertions in the ATPase domain and the central domain, a ~100 aa longer central domain; a ~200 aa shorter C-terminaldomain containing rich charged residues. These features revealed by comparing with Top 2 of the host, human, mightbe helpful in exploiting drug selectivity for antigiardial therapy. Phylogenetic analysis of eukaryotic enzymes showedthat kinetoplastids, plants, fungi, and animals were monophyletic groups, and the animal and fungi lineages shared amore recent common ancestor than either did with the plant lineage; microsporidia grouped with fungi. However, unlikemany previous phylogenetic analyses, the “amitochondriate” G. lamblia was not the earliest branch but diverged aftermitochondriate kinetoplastids in our trees. Both the finding of typical eukaryotic type IIA topoisomerase and the phylo-genetic analysis suggest G. lamblia is not possibly as primitive as was regarded before and might diverge after theacquisition of mitochondria. This is consistent with the recent discovery of mitochondrial remnant organelles in G. lamblia.

关键词： evolutionary position GlTop2 gene Giardia lamblia identification phylogenetic analysis type II DNAtopoisomerase.

Study on the Current Situation and Protection countermeasures of Wild Plant Resources in Xishuangbanna National Nature Reserve

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Meteorological and Environmental Research 2011年第4期2卷 79-82页

作者： YANG Zhong-xing1, YIN Wu-yuan2, AI Jian-lin1 1. Yunnan forestry Inventory and Planning Institute, kunming 650051, china 2. {3. forestry University, kunming 6502.4, china

[Objective] The aim was to study the current situation and protection countermeasures of wild plant resources in Xishuangbanna National Nature Reserve. [Method] The current situation of wild plant resources in Xishuangbanna National Nature Reserve was researched by means of route survey, sample plot survey and literature survey, and then the main impact factors of wild plant resources were analyzed by using participatory rural appraisal and problem tree analysis, finally protection countermeasures were put forward according to current situation and main impact factors. [Result] There were 2 779 species of vascular plants belonging to 214 families and 1 012 genera (including subfamilies and varieties) in Xishuangbanna National Nature Reserve, among them, there existed 261 species of pteridophyte belonging to 41 families and 91 genera, 14 species of gymnosperm belonging to 6 families and 6 genera, and 2 504 species of angiosperm belonging to 167 families and 915 genera. Wild plant resources in reserve were mainly affected by planting under forest, habitat fragmentation, cutting trees, collection and utilization of no-timber products, development of animal husbandry, insufficient recognition of community residents to reserve management establishment and lots of floating population. In the future, wild plant resources in reserve could be protected through strengthening propaganda, education and management, enforcing the law strictly and other countermeasures. [conclusion] The study could provide theoretical foundation for the protection of wild plant resources in Xishuangbanna National Nature Reserve.

关键词： Wild plant resources Protection countermeasures Impact factors National Nature Reserve Xishuangbanna china

Synergistic inhibition effect of rare earth Ce4+ and 3.4-dihydroxybenzaldehye on the corrosion of steel in H3.O4 solution

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Synergistic inhibition effect of rare earth Ce4+ and 3,4-dih...

Present Status of Francois' Langur(Trachypithecus francoisi) in china

第六届国际稀土开发与应用研讨会

作者： LI Xianghong~(1*),DENG Shuduan~2,FU Hui~1 (1.Department of Fundamental courses,southwest forestry University,kunming {3., china 2.Faculty of Wood Science and Decoration Technology,southwest forestry University,kunming {3.,china)

The synergism between rare earth Ce and 3.4-dihydroxybenzaldehye(DHBA) on the corrosion of cold rolled steel(CRS) in 1.0 M HPO solution at 20℃was first studied by weight loss,potentiodynamic polarization and electrochemical impedance spectroscopy(EIS) *** inhibited solutions were analyzed by ultraviolet and visible spectrophotometer (UV-vis).The results reveal that DHBA has a moderate inhibitive effect,and the maximum inhibition efficiency(E) is about 62%.The adsorption of DHBA obeys Temkin adsorption *** curves show that DHBA is a mixed-type inhibitor in phosphoric acid, while prominently inhibits the cathodic *** Ce,it has a negligible effect,and the maximum E is only about 15%.However,incorporation of Ce with DHBA improves significantly the inhibition *** E for Ce in combination with DHBA is higher than the summation of E for individual Ce and individual DHBA,which is synergism in ***-vis shows that the new complex of Ce—vanillin is formed in 1.0 M HPO for Ce combination with *** studies show that the complex of Ce—DHBA acts as a mixed-type inhibitor,which drastically inhibits both anodic and cathodic reactions. All EIS spectra exhibit one capacitive loop which indicates that the corrosion reaction is controlled by charge transfer process.A probable synergism mechanism is discussed from the viewpoint of adsorption theory.

关键词： Rare earth Steel corrosion Phosphoric acid Inhibitor Adsorption Synergism

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Present Status of Francois' Langur(Trachypithecus francoisi)...

第七届全国野生动物生态与资源保护学术研讨会

作者： Gang HU~(1,2,3.) (1 Institute of Rare Wildlife,Life Science co.lege,china West Normal University,Nanchong 63.009, china) 2 Kay Laboratory of {3. china Wildlife Resources conservation,Educational Ministry of china,Nachong 63.009,china 3.Institute of Wildlife,{3. forestry University,kunming 6502.4, china)

Francois’ langur,Trachypithecus francoisi,is noticeably widespread geographically,ranging from the Red River in northern Vietnam to south central china,about 21°3.’ to 29°08’N(Fooden,1996;Groves,2001).It is restr... 详细信息

关键词： conservation status Distribution dynamics Francois’ langur Trachypithecus francoisi Habitat degradation Population size

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菲律宾生物多样性现状及其保护策略

世界林业研究 1999年第4期12卷 67-70页

作者：刘思慧西南林学院林业系

快速的人口增长和迅速的植被破坏已成为当今菲律宾环境演化的主要趋势。近年滥伐森林已超过１０万ｈｍ２，由于木材采伐、林地遭农田蚕食和森林火灾，降低了森林覆盖率，改变了水文条件；森林砍伐也暗示着乡土植物和乡土动物不可逆转的... 详细信息

快速的人口增长和迅速的植被破坏已成为当今菲律宾环境演化的主要趋势。近年滥伐森林已超过１０万ｈｍ２，由于木材采伐、林地遭农田蚕食和森林火灾，降低了森林覆盖率，改变了水文条件；森林砍伐也暗示着乡土植物和乡土动物不可逆转的灭绝，导致了高价值栖息地和平衡生态系统的消失。在过去的３０年间，７０％的原始红树林全部被毁坏，大约６０％的特有种现已灭绝。关于菲律宾的动物，目前又有１８种陆栖脊椎动物被列为濒危种，明多罗水牛（Ｂｕｂａｌｕｓｍｉｎｄｏｒｅｎｓｉｓ）和菲律宾鹰（Ｐｉｔｈｅｃｏｐｈａｇａｊｅｆｅｒｙｉ）正处于灭绝的边缘。所有菲律宾国家公园由于人类的侵蚀已不同程度地降低等级。只有排名世界第三的礁石经调查后情况良好。文中叙述了菲律宾陆地、湿地和海洋的生物多样性，以及当前对生物多样性保护的策略和措施。

关键词：生物多样性菲律宾保护策略国家公园

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小鼠对不同拓扑性质图形的识别

生物化学与生物物理进展 2010年第6期37卷 613-617页

作者：朱静郭兴焱马园任菲西南林学院保护生物学学院昆明650224

视觉感知的一系列研究都支持大范围拓扑感知的理论.拓扑性质作为整体性质,是视觉感知的基础.视觉对图形拓扑特征差异的感知要优先于对局部特征差异的感知.采用Y迷宫研究了小鼠对不同拓扑性质图形的识别.训练小鼠学习识别圆环和实心矩形... 详细信息

视觉感知的一系列研究都支持大范围拓扑感知的理论.拓扑性质作为整体性质,是视觉感知的基础.视觉对图形拓扑特征差异的感知要优先于对局部特征差异的感知.采用Y迷宫研究了小鼠对不同拓扑性质图形的识别.训练小鼠学习识别圆环和实心矩形这一对拓扑性质不同的图形,之后用拓扑特征相同或不同的其他图形测试小鼠,这些图形包括空心矩形、实心圆、缺口的圆环、缺口的空心矩形.实验结果表明,学会识别圆环(奖励)和实心矩形(无奖励)的小鼠无法区分实心圆和实心矩形以及圆环和空心矩形,但是能够分别从缺口圆环、缺口的空心矩形、实心圆与空心矩形组成的图形对中识别出空心矩形.因此证实了小鼠的视觉系统能够感知拓扑特征的差异并且具有对拓扑性质的概括能力.结果为拓扑知觉对视觉系统来说是基本的这一假设提供了证据.

关键词：小鼠拓扑知觉理论 Y迷宫

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Sleeping Behavior of Wild Francois' Langur(Trachypithecus francoisi) at Mayanghe Nature Reserve in Summer

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Sleeping Behavior of Wild Francois' Langur(Trachypithecus fr...

第七届全国野生动物生态与资源保护学术研讨会

作者： Han Jialiang~(1,2),Hu Gang~(1,2,3.) (1 Institute of Rare Wildlife,Life Science co.lege,china West Normal University,Nanchong 63.009, china 2 Kay Laboratory of {3. china Wildlife Resources conservation,Educational Ministry of china,Nachong 63.009,china 3.Institute of Wildlife,{3. forestry University,kunming 6502.4, china)

During the period from July to August 2011,we recorded the sleeping behavior,including the time allotting for entrance and exit sleeping site,pre-sleeping and sleeping,of two groups of wild Francois’ langur at Mayang... 详细信息

关键词： Francois’ langur(Trachypithecus francoisi) sleeping behavior summer Mayanghe Nature Reserve

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高原湿地若尔盖国家级自然保护区景观变化

山地学报 2010年第2期28卷 240-246页

作者：邓茂林田昆段宗亮周耀华杨佼王进琼西南地区生物多样性保育国家林业局重点实验室西南林学院环境科学与工程系国家高原湿地研究中心

以1990年和2000年及2007年landsat5TM影像解译出的景观格局图为主要数据源,应用FRAGSTATS软件对景观斑块数、斑块面积百分比、Shannon多样性指数和Shannon均匀度等7个景观格局指数及景观类型转移矩阵定量,分析近20年来若尔盖保护区景观... 详细信息

以1990年和2000年及2007年landsat5TM影像解译出的景观格局图为主要数据源,应用FRAGSTATS软件对景观斑块数、斑块面积百分比、Shannon多样性指数和Shannon均匀度等7个景观格局指数及景观类型转移矩阵定量,分析近20年来若尔盖保护区景观格局动态变化及其驱动因子。结果表明:17年来,若尔盖国家级自然保护区的斑块数、斑块面积百分比、聚合度、Shannon多样性指数和Shannon均匀度都发生了较大变化,景观尺度下退化沼泽为景观基底占优势的景观类型,2007年占全区总面积比例48.05%,该区在人类活动影响下,景观破碎度和异质性先降低后期增加,优势度增加后降低。1990～2000年,湖沼和河流水面积减少,沙地治理,退化沼泽、草甸和灌木面积分别增加了1978.60hm2、2559.09hm2和824.27hm2,2000～2007年,湖沼水面积仍在退缩,退化沼泽、草甸类的草场面积持续增加,沙地显著增加1945.90hm2。气候变暖叠加人为排水疏干、过度放牧、无序旅游、管理局的保护管理及基础建设等是保护区湿地景观格局变化的主要驱动因素。

关键词：高原湿地若尔盖景观格局变化驱动力