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Effect of transforming growth factor-beta on activity of connective tissue growth factor gene promoter in mouse NIH/3.3.fibroblasts

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Acta Pharmacologica Sinica 2004年第4期25卷 87-91页

作者： Qing ZHAO, Nan CHEN2, Wei-ming WANG, Jian LU3.Bing-bing DAI3. Department of Nephrology, ruijin hospital 3.epartment of Biochemistry, shanghai Second Medical University,{3., china Department of Nephrology Ruijin Hospital

AIM: To investigate the regulatory mechanism of transforming growth factor-betaon activity of connective tissue growth factor promoter in mouse NIH/3.3.fibroblasts. METHODS: The regulation fragment of the 5' flanking region of the human CTGF gene was linked to pGL3.Basic vector, a firefly luciferase reporter construct without promoter. The recombinant plasmid pCTGF-luc was transiently transfected to NIH/3.3.fibroblasts. The activity of CTGF promoter after treatment of TGF-β1 and MAPK pathway inhibitors were assayed with luciferase reporter gene assay system. RESULTS: TGF-β1-induced increase of CTGF promoter activity was concentration-dependent, with a plateau at 5 μg/L by 2.67-fold vs control (P3.80 (10 μmol/L), the p3. MAP kinaseinhibitor, decreased basal and TGF-β1-induced activation of CTGF promoter. However, inhibition of c-Jun-N-terminal kinase/stress-activated protein kinase by SP600125 (20 μmol/L) was without effect. CONCLUSION: TGF-β1stimulated the transcriptional activity of CTGF gene promoter in NIH/3.3.fibroblasts in a dose- and time-dependent manner. MAPK pathway may play a role in the regulation of TGF-β1-induced CTGF expression.

关键词： transforming growth factor-beta connective tissue growth factor promoter mitogen-activated protein kinases signal transduction

Expression of Hepatitis C Virus E2 Ectodomain in *** and Its Application in the Detection of Anti-E2 Antibodies in Human Sera

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 57-63页

作者： Jing LIU, Xin-Xin ZHANG, Shen-Ying ZHANG, Min LU, Yu-Ying KONG, Yuan WANG, and Guang-Di LI ( State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, shanghai 20003., china 2 Department of Infectious Diseases, {3. hospital, Second Medical University of shanghai, shanghai 200025, china ) State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 Chin

The second envelope glycoprotein （E2） of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment （aa 3.5-73.） with a four-amino-acid mutation （aa 568-571 PCNI to RVTS） was expressed as hexa-histidine-tagged full length protein [E2N73.（m）] in E. coli and purified to over 85% purity. Purified E2N73.（m） was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2specific antigenicity. Rabbit antiserum raised against E2N73.（m） recognized E2 glyco-proteins expressed in mammalian cells in Western blot. Purified E2N73.（m） was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previousl

关键词： hepatitis C virus envelope protein E2 expression and purification Escherichia coll

Laparoscopic low anterior resection for rectal carcinoma:Complications and management in 13. consecutive patients

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世界胃肠病学杂志：英文版 2010年第36期16卷 4605-4610页

作者： Qian-Lin Zhu,Bo Feng,Ai-Guo Lu,Ming-Liang Wang,Wei-Guo Hu,Jian-Wen Li,Zhi-Hai Mao,Min-Hua Zheng,Department of General Surgery,shanghai ruijin hospital,shanghai Jiao Tong University School of Medicine,shanghai 200025,china shanghai Minimally Invasive Surgery Center,shanghai 200025,china Department of General Surgery Shanghai Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China. zqlalani@***

AIM:To analyze the clinical manifestations and risk fac-tors of complications in laparoscopic low anterior resection(LAR)for rectal cancer ***:A series of 13. consecutive patients who received laparoscopic LAR for rectal cancer in our center were *** etiology,diagnosis,treatment and prevention of rectal cancer were studied among the patients with surgery-related complications using both univariate and multivariate regression ***:No conversion to open surgery was observed and 5 cases converted to hand-assisted laparoscopic *** overall morbidity rate was 20.5%.Complications occurred during the operation in 7 patients(5.3.),within 3. postoperative days in 24 patients(18.2%),and within 3.mo in 2 patients(1.5%).The most significant complications were anastomotic leak-age(9.1%)and anastomotic hemorrhage(5.3.).Sizeand location of tumor,pathological staging and preoperative nutrition were significant factors associated with LAR complications,while gender,age and pathological type showed no *** logistics regression showed that the size and location of tumor,and pathological staging were independent factors of laparoscopic *** the complications were treated during their onset of clinical manifestations by interventional or conservative ***:Anastomotic leakage is a major com-plication in laparoscopic *** complications may be associated with tumor size and site,and pathological *** therapies are of value in the management of laparoscopic LAR complications.

关键词： Laparoscopy Low anterior resection Complication Rectal cancer Logistic regression analysis

Hepatocyte differentiation of mesenchymal stem cells

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 360-371页

作者： Xu-Bo Wu Department of General Surgery,Central hospital of Minhang District,ruijin Medical Group,shanghai 201199,{3. Ran Tao Center for Organ Transplantation,Department of General Surgery,ruijin hospital,shanghai Jiaotong University School of Medicine,shanghai 200025,{3. [a] Department of General Surgery Central Hospital of Minhang District Ruijin Medical Group Shanghai 201199 China [b] Center for Organ Transplantation Department of General Surgery Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai 200025 China

BACKGROUND:Liver cell transplantation and bioartificial liver may provide metabolic support of liver function temporary and are prospective treatments for patients with liver *** stem cells(MSCs) are expected to be an ideal cell source for transplantation or liver tissue engineering,however the hepatic differentiation of MSCs is still insufficient for clinical *** SOURCES:A PubMed search on 'mesenchymal stem cells','liver cell' and 'hepatocyte differentiation' was performed on the topic,and the relevant articles published in the past ten years were ***:Hepatocyte-like cells differentiated from MSCs are a promising cell source for liver regeneration or tissue *** it is still a matter of debate as to whether MSC-derived hepatocytes may efficiently repopulate a host liver to provide adequate functional substitution,the majority of animal studies support that MSCs can become key players in liver-directed regenerative *** the clinical application of human stem cells in the treatment of liver diseases is still in its ***:Future studies are required to improve the efficacy and consistency of hepatic differentiation from *** is necessary to better understand the mechanism to achieve transdifferentiation with high *** clinical trials are warranted to prove their efficacy in the management of patients with liver failure.

关键词： hepatocyte differentiation mesenchymal stem cells

Usefulness of an algorithm for endoscopic retrieval of proximally migrated 5Fr and 7Fr pancreatic stents

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 196-200页

作者： Biao Gong,Bo Sun,Li-Xiao Hao and Like Bie Department of Gastroenterology,ruijin hospital, shanghai Jiaotong University School of Medicine,shanghai 200025,china Department of Gastroenterology,shanghai Gleneagles Medical Center,Parkway Health,shanghai 200003.china Department of Hepatobiliary Surgery,No.455 hospital,shanghai 200025, china [a] Department of Gastroenterology Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai 200025 China [b] Department of Gastroenterology Shanghai Gleneagles Medical Center Parkway Health (North Asia) Shanghai 200003 China [c] Department of Hepatobiliary Surgery No. 455 Hospital Shanghai 200025 China

BACKGROUND:Proximal migration of pancreatic stent (PMPS)is an infrequent event but its management can be technically challenging and there are no standard retrieval *** study aimed to determine the results of an endoscopic stent retrieval algorithm in terms of feasibility and efficacy of the endoscopic procedure. METHODS:During the period from January 2008 to December 2009,15 patients(8 women and 7 men with a mean age of 51.9 years)with PMPS were included in this *** retrieval was approached initially with balloon extraction followed by rat-tooth forceps and basket.A rescue approach such as using a stent retriever was attempted when other approaches failed. RESULTS:All the PMPSs(six 5Fr,nine 7Fr)were retrieved successfully within one ERCP *** extraction was successful in 9(60%)*** the 6 failed cases of balloon extraction,wire-guided rat-tooth forceps grasp was successful in 4,and stone extraction basket grasp was successful in 1 in whom forceps grasp *** stent was finally rescued with a stent retriever when balloon extraction,forceps and basket grasp all *** patients with successful balloon extraction,44.4% (4/9)developed post-ERCP hyperamylasemia but none of them developed post-procedure pancreatitis. CONCLUSIONS:With this algorithm,5Fr and 7Fr PMPS were successfully retrieved in all of the *** PMPSs can be safely retrieved with the commonly-used approaches in this *** less used approaches can be used as a rescue method.

关键词： algorithm endoscopic retrieval pancreatic stents

Association of DRD2 polymorphisms and chlorpromazine-induced extrapyramidal syndrome in Chinese schizophrenic patients

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Acta Pharmacologica Sinica 2006年第8期27卷 966-970页

作者： Sheng-nan WU~(2,3.5,6) Rui GAO~(2,3.6) Qing-he XING~(2,3.6) Hua-fang LI~4 Yi-feng SHEN~4 Niu-fan GU~4 Guo-yin FENG~4 Lin HE~(2,3.7)2 Bio-X Life Science Research Center,shanghai Jiaotong University,shanghai 20003.,china 3.Institute of Nutritional Sciences,SIBS,Chinese Academy of Sciences,shanghai 20003.,china 4 shanghai Institute of Mental Health,shanghai 20003.,china 5 shanghai Institute of Hypertension,ruijin hospital,Medical School of shanghai Jiaotong University,shanghai {13.,china Bio-X Life Science Research Center Shanghai Jiaotong University Shanghai 200030 China

Aim:Extrapyramidal syndrome (EPS) is most commonly affected by typical antip-sychotic drugs that have a high affinity with the D2 ***,manyresearch groups have reported on the positive relationship between the geneticvariations in the DRD2 gene and the therapeutic response in schizophrenia pa-tients as a result of the role of variations in the receptor in modulating *** this study,we evaluate the role DRD2 plays in chlorpromazine-induced EPS in schizophrenic ***:We identified seven SNP(singlenucleotide polymorphism) (-141Cins>del,TaqIB,TaqID,Ser3.1Cys,rs6275,rs6277and TaqIA) in the DRD2 gene in 146 schizophrenic inpatients (59 with EPS and 87without EPS according to the Simpson-Angus Scale) treated with chlorpromazineafter 8 *** alleles of all loci were determined by PCR (polymerase chainreaction).Results:Polymorphisms TaqlD,Ser3.1Cys and rs6277 were not poly-morphic in the population recruited in the present *** statistical signifi-cance was found in the allele distribution of-141Cins>del,TaqIB,rs6275 andTaqIA or in the estimated haplotypes (constituted by TaqIB,rs6275 and TaqIA) inlinkage disequilibrium between the two ***:Our results did notlend strong support to the view that the genetic variation of the DRD2 gene playsa major role in the individually variable adverse effect induced by chlorpromazine,at least in Chinese patients with *** results confirmed a previousstudy on the relationship between DRD2 and EPS in Caucasians.

关键词： dopamine receptors basal ganglia disease polymorphism chlorpromazine schizophrenia

多剂量包装中药配方颗粒调剂管理上海共识（2023.

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上海中医药大学学报 2024年第5期 1-9页

作者： Clinical Chinese Materia Medica Group of hospital Pharmacy Professional Committee of shanghai Pharmaceutical Association hospital Pharmacy Branch of shanghai Association of Traditional Chinese Medicine Writing Group of"shanghai Consensus on Dispensing Management of Multi-dose Packaged Traditional Chinese Medicine Formula Granules (2023." 上海市药学会医院药学专业委员会临床中药学组上海市中医药学会医院药学分会《多剂量包装中药配方颗粒调剂管理上海共识（2023.》编写组

多剂量包装中药配方颗粒利用设备进行调剂，在上海各医疗机构使用广泛，但药品的调剂质量受多方面因素影响较大，管理要求亟待完善提高。为此，上海市药学会医院药学专业委员会临床中药学组等单位组织专家，根据有关政策法规要求，参考... 详细信息

多剂量包装中药配方颗粒利用设备进行调剂，在上海各医疗机构使用广泛，但药品的调剂质量受多方面因素影响较大，管理要求亟待完善提高。为此，上海市药学会医院药学专业委员会临床中药学组等单位组织专家，根据有关政策法规要求，参考相关标准规范和近5年的文献报道，结合上海市实际情况，围绕与多剂量包装中药配方颗粒调剂相关的人员要求、温湿度控制、设备要求、清洁污染、效期管理、发药交待等管理问题，以“陈述”形式形成上海地区多剂量包装中药配方颗粒调剂管理专家共识，以期进一步规范中药配方颗粒的临床调剂工作，加强应用的安全性、合理性和可控性。

关键词：中药配方颗粒多剂量包装调剂专家共识上海

CT manifestations and features of solid cystic tumors of the pancreas

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 465-468页

作者： Fei Miao Ying Zhan Xiao-Ying Wang Deng-Bin Wang Ke-Ming Chen Ao-Rong Tang Hao Jiang the Department of Radiology, ruijin hospital, shanghai 200025,{3. Department of Pathology, ruijin hospital. shanghai 200025.{3. Department of Radiology Ruijin Hospital Shanghai 200025 China.

Objective: To study retrospectively the manifestations and characteristics of solid cystic tumor of the pan- creas(SCTP) on CT. Methods: Nine patients with solid cystic tumor of the pancreas underwent resection with pathological and immunohistochemical confirmation. All the patients were female, aged 27.7 years on average at onset of the disease. CT scan was performed in patients with or without intravenous injection of contrast medium; it was also performed in the arterial phase in 4 pa- tients. Results: Solid and cystic structures were observed in tumors of the pancreas. Solid structure demonstrated low or iso-density on unenhanced CT, poor enhance- ment in the arterial phase and marked enhancement in the portal venous phase on contrast material-en- hanced CT. Cystic structure showed low density on both pre- and pro-contrast views. The CT findings of tumors with predominantly cystic or equal propor- tion of solid and cystic structure: mural nodules for solid structure, 'floating cloud' signs, or solid and cystic crossing distributions. Cystic structure of tumors predominately solid presenting as a beading appearance under capsule. In round, oval and com- pletely encapsulated tumors in the pancreas, the greater vessel enhanced with well-defined margins on pro-contrast images. No dilatation was observed of the common bile and pancreatic ducts. Conclusion: The characteristics of solid cystic tumor of the pancreas revealed by CT could confirm the di- agnosis of the disease in combination with onset age and sex of patients.

关键词： pancreatic tumors computed tomography diagnosis

Effects of ketamine, midazolam, thiopental, and propofol on brain ischemia injury in rat cerebral cortical slices

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Acta Pharmacologica Sinica 2004年第1期 117-122页

作者： Qing-sheng XUE, Bu-wei YU, Ze-jian WANG, Hong-zhuan CHENDepartment of Anesthesiology, ruijin hospital Department of Pharmacology, shanghai Second Medical University,{3., china

AIM: To compare the effects of ketamine, midazolam, thiopental, and propofol on brain ischemia by the model of oxygen-glucose deprivation (OGD) in rat cerebral cortical slices. METHODS: Cerebral cortical slices were incubated in 2 % 2,3.5-triphenyltetrazolium chloride (TTC) solution after OGD, the damages and effects of ketamine, midazolam, thiopental, and propofol were quantitativlye evaluated by ELISA reader of absorbance (A) at 490 nm, which indicated the red formazan extracted from slices, lactic dehydrogenase (LDH) releases in the incubated supernate were also measured. RESULTS: Progressive prolongation of OGD resulted in decreases of TTC staining. The percentage of tissue injury had a positive correlation with LDH releases, r=0.9609, P<0.01. Two hours of

关键词： brain ischemia cerebral cortex ketamine midazolam thiopental propofol 2,3.5-triphenyltetrazolium