检索结果-南通市图书馆

Calculation of the Surface Segregation of Cu-0.3.at. pct Au Alloy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2000年第6期16卷 639-640页

作者： Liping ZHENG and Risheng LI (International Center for Material Physics, chinese academy of sciences, Shenyang 110015, china) Yuanxun ZHANG and Shi QIU (shanghai {3. of Nuclear Research, chinese academy of sciences, shanghai 201800, china) Bingyao JI Int. Cent. for Material Phys. Chinese Acad. of Sci. Shenyang 110015 China

Monte Carlo simulation technique with the energetics described by the embedded atom method has been used to calculate the surface segregation of Cu-0.3.at. pct Au alloy at T=800 K. It is found that the component Au in... 详细信息

关键词： Cu Calculation of the Surface Segregation of Cu-0.3.at pct Au Alloy

Effect of osmotic shock on the redox system in plasma membrane of Dunaliella salina

在线全文

学校读者我要写书评

暂无评论

Cell Research 1996年第1期6卷 31-38页

作者： CHEN SI XUE CHI CHIONG YEN XIN ZHI JIAO (institute of plant physiology,{3., shanghai 20003., china.)(Biology Department, East china Normal University,shanghai 200062, china.) Biology Department East China Normal University Shanghai 200062 China.

The unicellular halotolerant alga Dunaliella salina had the ability to oxidize NADH and reduce Fe(CN)63.. The redox reactions were to some extent stimulated by slight hyperosmotic shock (2.0 mol/L → 2.6 mol/L NaCl), butmarkably inhibited by abrupt hyperosmotic shock (2.0mol/L → 3.5 mol/L NaCl) and hypoosmotic shock (2.0mol/L → 1.0 mol/L NaCl; 2.0 mol/L→0.67 mol/L NaCl).With the adaptation of algal cells to osmotic shock by accumulating or degrading intracellular glycerol, the plasmalemma redox activities were also restored. The O2 uptake stimulated by NADH could be promoted by FA and SHAM. Hypoosmotic shock increases the basal respiration rate of alga cells, but weakened the stimulating effects of NADH, FA and SHAM on O2 uptake. On the other hand, hyperosmotic shock reduced the basal respiration rate, but relatively enhanced the above effects of NADH, FA and SHAM. H+ extrusion of alga cells was inhibited by NADH and stimulated by Fe(CN)63. Vanadate and DES could inhibit H+ efflux, but had little effect in the presence of NADH and Fe(CN)63.. Both hyperand hypoosmotic shock stimulated H+ extrusion. This effect could be totally inhibited by vanadate and DES, but almost unaffected by 8-hydroxyquinoline. It was suggested that H+-ATPase probably played a more important role in H+ extrusion and osmoregulation under the conditions of osmotic shock.

关键词： Osmotic shock Dunaliella salina plasmalemma redox system

Virtual multi-dimensional internal bonds model and its application in simulation of rock mass

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Technological sciences) 2008年第2期51卷 192-201页

作者： ZHANG ZhenNan1,2 & GE XiuRun3.4 1 Department of Civil Engineering,shanghai University,shanghai 200072,china 2 shanghai institute of Applied Mathematics and Mechanics,shanghai University,shanghai 200072, china 3.institute of Geotechnical Engineering,shanghai Jiao Tong University,shanghai 20003.,china 4 institute of Rock and Soil Mechanics,chinese academy of sciences,Wuhan 43.071,china 1. Department of Civil Engineering Shanghai University Shanghai 200072 China 2. shanghai institute of Applied Mathematics and Mechanics Shanghai University Shanghai 200072 China 3. institute of Geotechnical Engineering Shanghai Jiao Tong University Shanghai 200030 China 4. institute of Rock and Soil Mechanics Chinese Academy of Sciences Wuhan 430071 China

VMIB (virtual multi-dimensional internal bonds) is a multiscale mechanical model developed from the VIB (virtual internal bond) theory. In VIB theory,the solid mate-rial is considered to consist of random-distributed material particles in microscale. These particles are connected with normal bonds. The macro constitutive relation is derived from the cohesive law between particles. However,in VMIB,the micro particles are connected with both normal and shear bonds. The macro constitutive relation is derived in terms of bond stiffness coefficients. It has been theoretically certified that there exists a corresponding relationship between the two bond stiffness coefficients and the two macro material constants,i.e. the Young’s modulus and Poisson ratio. This corresponding relationship suggests that it should be necessary and sufficient to simultaneously account for the normal and shear interactions between particles. Due to the fact that the fracture criterion is directly incorporated into the constitutive relation,both VIB and VMIB present many advantages in simulating fractures of materials. In the damage model of rock mass,a damage tensor is usually defined to describe the distribution of cracks. The damage value in one direction determines the relative stiffness of rock mass in this direction. In VMIB solid,the relative distribution density of micro bonds in one direction determines the relative macro stiffness of the material in this direction. The effects of the damage value and the relative distribution density of bonds are consistent. To simulate the failure behavior of rock mass with VMIB,the presented paper sets up a quantitative relationship between the damage tensor and the rela-tive distribution density of bonds. Comparison of the theoretical and the experi-mental results shows that VMIB model can represent the effect of distributed cracks on rock mass with this relationship. The presented work provides a founda-tion for further simulating fracture behavior

关键词： VMIB (virtual multi-dimensional internal bonds) rock mass VIB (virtual internal bonds) multi-cracked body numerical simulation

<100> Textured Diamond Film on Silicon Grown by Hot Filament Chemical Vapor Deposition

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 1995年第6期11卷 426-428页

作者： Xuanxiong ZHANG Tiansheng SHI and Xikang ZHANG (State Key Laboratory of Functional Materials for Informatics, shanghai institute of Metallurgy,chinese {3. of sciences, shanghai, 200050, china) shanghai Inst of Metallurgy Chinese Acad of Sciences Shanghai China

The textured growth of diamond film on HF eroded silicon wafer has been studied by HFCVD. The evolution of grain size and sudece morphology vs deposition time is presented and the textured thick diamond film (80μm)... 详细信息

关键词： Textured Diamond Film on Silicon Grown by Hot Filament Chemical Vapor Deposition OO

Detection of Tumor Marker CA125 in Ovarian Carcinoma Using Quantum Dots

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第10期36卷 681-686页

作者： Hui-Zhi WANG, Hai-Yan WANG, Ru-Qiang LIANG, and Kang-Cheng RUAN* Key Laboratory of Proteomics, institute of Biochemistry and Cell Biology, shanghai institutes for Biological {3.,chinese academy of {3., Graduate School of the chinese academy of sciences, shanghai 20003., china Key Laboratory of Proteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

Semiconductor quantum dots (QDs) offer several advantages over organic dyes in fluores- cence-imaging applications, such as higher quantum yield, exceptional photostability, and a narrow, tunable, and symmetric emission spectrum. To explore whether QDs could specifically and effectively label tumor markers and be used in immunohistochemistry as a novel type of fluorescent probe, we used quantum dots with maximum emission wavelength 605 nm (QD605) to detect the ovarian carcinoma marker CA125 in specimens of different types (fixed cells, tissue sections, and xenograft piece). Additionally, we compared the photostability of QD signals with that of a conventional organic dye, FITC. All labeling signals of QDs were found to be more specific and brighter than those of FITC. Moreover, the QDs exhibited exceptional photostability during continuous illumination for 1 h by a high-intensity laser (Ar laser power 100 mW) at 488 nm, while the FITC signals faded very quickly and became undetectable after 24 min of illumination. These results indicate that QD-based probes can offer substantial advantages over existing fluorophores in many applications, and can be used effectively in immunohistochemistry as a novel class of fluorescent probes.

关键词： quantum dots detection of tumor marker CA125 ovarian carcinoma

Predominant expression of β-chain variable region genes of T-cell receptor in TILs from human renal cell carcinoma

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

chinese Science Bulletin 1997年第8期42卷 693-697页

作者： LI Xueyi, WANG Ying, ZHENG Zhongcheng, ZHANG Dongqing, FUSaili, LING Jianyu and ZHOU Guangyan (CHOU Kuangyen)1. shanghai institute of Immunology, shanghai Second Medical University, shanghai 200025, china 2. shanghai institute of Biochemistry, chinese academy of sciences, shanghai 20003., china 3. shanghai Ruijin Hospital, shanghai 200025, china shanghai institute of Immunology Shanghai Second Medical University Shanghai China shanghai institute of Biochemistry Chinese Academy of Sciences Shanghai China shanghai Ruijin Hospital Shanghai China

THE repertoire of T-cell antigen receptor(TcR)can be effectively analysed by an approachwith quantitative polymerase chain reaction(qPCR),which allows to characterize the ex-panding T-cell clones by the patterns of re... 详细信息

关键词： renal cell carcinoma tumor-infiltrating lymphocyte T-cell receptor Vβ gene quantitative polymerase chain reaction.

Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

在线全文

学校读者我要写书评

暂无评论

Cell Research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI HONG XU, HONG WEI XUE, National Laboratory of plant Molecular Genetics, institute of plant {3. and Ecology, shanghai institutes for Biological Science (SIBS), The chinese academy of sciences Partner Group of Max-Planck-institute of Molecular plant {3. (MPI-MP) on "plant Molecular {3. and Signal Transduction", 3.0 Fenglin Road, 20003. shanghai, china 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3.-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562 bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

Nucleic Acid Binding Activity of Pns6 Encoded by Genome Segment 6 of Rice Ragged Stunt Oryzavirus

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第7期36卷 457-466页

作者： Chao-Gang SHAO#, Hui-Juan Lü#, Jian-Hua WU, and Zu-Xun GONG* Key Laboratory of Proteomics, institute of Biochemistry and Cell Biology, shanghai institutes of Life {3., chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 20003., china Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes of Life Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200031 China

The ORF of genome segment 6 (S6) of rice ragged stunt oryzavirus (RRSV) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate. Pns6, the 71 kD product of S6 expressed in E. coli, was demonstrated to be a viral non-structural protein of RRSV by Western blotting. The gel mobility shift assays showed that Pns6 had nucleic acid binding activity. Pns6 could interact with single- and double-stranded forms of DNA and RNA, showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA, as demonstrated by both competition and displacement assays. The binding of Pns6 to nucleic acids is strong and sequence non-specific. By using five truncated derivatives of Pns6, it was found that the basic region from amino acid 201 to 273.of Pns6 was the unique nucleic acid binding domain. Subcellular fractionation of leaf tissues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction. The possible role of RRSV Pns6 in virus replication and assembly is discussed.

关键词： rice ragged stunt oryzavirus (RRSV) nucleic acid binding non-structural protein S6

Six Specific Lysihe Residues are Crucial in Maintaining the Structure and Function of Soluble Manganese Stabilizing Protein

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第9期 611-619页

作者： Jin-Peng GAO~(1,2#) Feng ZHANG~(1,3.) Li ZHANG~4 Yin-Long GUO~4 Kang-Cheng RUAN~5 De-An JIANG~3. Chun-He XU~1 1 institute of plant physiology and Ecology,{3. institutes for Biological sciences,chinese academy of sciences,{3. 20003.,china 2 Graduate School of the chinese academy of sciences,{3. 20003.,china 3.College of Life sciences,Zhejiang University,Hangzhou 3.0029,china 4 {3. Mass Spectrometry Center,{3. institute of Organic Chemistry,chinese academy of sciences,{3. 20003.,china 5 Laboratory of Proteomics,institute of Biochemistry and Cell Biology,{3. institutes for Biological sciences,chinese academy of sciences,{3. 20003.,china

When manganese stabilizing protein （MSP） was treated with 0.5mM N-succinimidyl propionate（NSP）,the rebinding ability and oxygen-releasing capabilities of the modified MSP were not altered,in spiteof changes of MSP surface Lys ***,far-ultraviolet circular dichroism and intrinsicfluorescence spectra analysis revealed that 0.5 mM NSP-modified MSP retained most of its native secondaryand tertiary *** of the sites of NSP modification by Staphylococcus V8protease digestion ofthe modified protein,as well as analysis by matrix-assisted laser desorption ionization-time of flight massspectrometry,indicated that seven Lys residues were *** results suggested that these residues arenot absolutely essential to the structure and function of ***,when the NSP concentration wasincreased to 4mM,the modified MSP was unable to bind photosystem Ⅱ and completely lost its *** far-ultraviolet circular dichroism and intrinsic fluorescence spectra analysis revealed a clearconformational change in MSP after 4 mM NSP treatment,suggesting that some Lys residues are involved inmaintaining the structure and function of *** by matrix-assisted laser desorption ionization-timeof flight mass spectrometry indicated that another six Lys residues,namely Lys20,Lys101,Lys196,Lys207,Lys13. （or Lys13.） and Lys66 （or Lys76）,were modified by 4 mM ***,these six Lys residuesare crucial in maintaining the structure and function of soluble MSP.

关键词： chemical modification fluorescence spectrum circular dichroism spectrum manganese stabilizing protein Lys residue