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γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology

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Cell Research 2000年第1期10卷 59-69页

作者： MA YING HUA JIA HUA HU XIAO GANG ZHOU ZHEN TONG MEI JIAN FEI LI HE GUO(shanghai institute of Cell Biology, chinese {3. of Science, shanghai 20003., china shanghai institute of physiology, chinese {3. of Science, shanghai 20003., china) shanghaiinstituteofCellBiology ChineseAcademyofScience.

γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid transporter was implicated to be involved in this process. However, the potential role of γ-aminobutyric transporter in testis has not been explored. In this study, we investigated the existence of mouse γ-aminobutyric acid transporter subtype I (mGAT1) in testis. Wild-type and transgenic mice, which overexpressing mGAT1 in a variety of tissues, especially in testis, were primarily studied to approach the profile of mGAT1 in testis. Mice with overexpressed mGAT1 develop normally but with reduced mass and size of testis as compared with wild-type. Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen. Reduced number of spermatids was also found in some seminiferous tubules. Our results clearly demonstrate the presence of GAT1 in mouse testis and imply that GAT1 is possibly involved in testicular function.

关键词： γ-aminobutyric acid transporter testicular morphology mouse.

Molecular cloning and primary sequence analysis of a gene encoding a putative chitinase gene in Brassica oleracea ***

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Cell Research 1996年第1期6卷 65-73页

作者： TANG GUO QING YONG YAN BAI SHI WEI LOO(shanghai institute of plant {3., chinese academy of sciences, 3.0 Fenglin Road shanghai, china 20003..) shanghai institute of plant {3. Chinese Academy of Sciences China

Chitinase, which catalyzes the hydrolysis of the β-1,4-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible plants defense system. By construction of cabbage (Brassica oleracea ***) genomic library and screening the library with pRCH8, a probe of rice chitinase gene fragment, a chitinase genomic sequence was isolated. The complete nucleotide sequence of the putative cabbage chitinase gene (cabch29) was determined, with its longest open reading frame (ORF)encoding a polypeptide of 413.aa. This polypeptide consists of a 21 aa N-terminal signal peptide,two chitin-binding domains different from those of other classes of plant chitinases, and a catalytic domain. Homology analysis illustrated that this cabch29 gene has 58.8% identity at the nucleotide level with the pRCH8 ORF probe and has 50% identity at the amino acid level with the catalytic domains of chitinase from bean,maize and sugar beet. Meanwhile,several kinds of cis- elements,such as TAT.A box, CAAT box, GATA motif, ASF-1 binding site, wound-response elements and AATAAA, have also been discovered in the flanking region of cabch29 gene.

关键词： Cloning sequence cabbage chitinase gene

Study on the stannous halides promoted addition of 1,1,1-trichloro-2,2,2-trifluoroethane to aldehydes and the chemical transformation thereof

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chinese Journal of Chemistry 1994年第2期12卷 183-189页

作者： HU, Chang-Ming CHEN, Jianshanghai institute of Organic Chemistry, chinese academy of sciences,{3., china shanghai institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

Promoted by stannous salts, 1,1,1-trichloro-2,2,2-trifluoroethane reacts readily with aliphatic, aromatic, and α, β-unsaturated aldehydes giving the corresponding alcohols bearing a CF3.Cl2-moiety in good to excelle... 详细信息

关键词： 1,1,1-Trichloro-2,2,2-trifluoroethane aldehydes stannous halides addition Jones oxidation ketones.

SYNTHESIS AND CHARACTERIZATION OF A NOVEL FLUORINE-CONTAINING HYDROPHOBICALLY ASSOCIATING POLYMER

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chinese Journal of Polymer Science 1999年第3期17卷 281-288页

作者： Jin Yang Wei-yuan Huang Laboratory of Organofluorine Chemistry, shanghai institute of Organic Chemistry, chinese academy of sciences,{3., china chinese Acad Sci Shanghai Inst Organ Chem Lab Organofluorine Chem Shanghai 200032 Peoples R China

In this article, the synthesis and characterization of a novel fluoromonomer and its copolymer with acrylamide is reported. 2-perfluoroamyl-4-hydroxylquinoline 2 was synthesized from ethyl 2, 2-dihydroperfluoroheptanoate in high yields. The monomer 4 was then synthesized from 2 in two steps readily in high yields. Synthesis and characterization of copolymers of acrylamide (AM) and the fluoromonomer were investigated. The composition and intrinsic viscosity of these copolymers were studied. It was found that the rheological properties of aqueous solutions of polyacrylamide were modified significantly when a small proportion of the fluoromonomer 4 was incorporated on investigating the viscosity-concentration profiles, pseudoplasticity of these solutions and the effect of the presence of surfactant. These results could be explained by the hydrophobic association of the fluorocarbon segments in the aqueous solutions of these copolymers.

关键词： hydrophobically associating polymer fluorine-containing acrylate perfluoroalkylation with RFI/Na2S2O4

Ultrafast excitation relaxation in light-harvesting complex LH2 from *** 601

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Science china Chemistry 2004年第3期47卷 192-199页

作者： GUO Lijun1,2, LIU Yuan3. LIU Weimin2, GUO Junhua1, XU Chunhe3.& QIAN Shixiong2 1. School of Physics and Information Optoelectronics, Henan University, Kaifeng 475001, china 2. Physics Department, Fudan University, shanghai 20043., china {3., chinese academy of sciences, shanghai 20003., china 1. School of Physics and Information Optoelectronics Henan University 475001 Kaifeng China 2. Physics Department Fudan University 200433 Shanghai China 3. shanghai institute of plant physiology Chinese Academy of Sciences 200032 Shanghai China

The energy relaxation and kinetic evolution of transient spectra of bacteriochloro- phylls (BChls) in light-harvesting complex LH2 from Rb. sphaeroides 601 were investigated using femtosecond pump-probe technique. Upon 783.nm excitation, the energy at B800 BChls ex-periences an intramolecular redistribution with 0.3. ps time constant before transferring to B850 BChls. With tuning the excitation wavelength, the dynamical evolution of excited BChls was clearly observed, which indicates an obvious competition between the ground state bleaching and excited state absorption (ESA) of BChls involved and an isosbestic point near 818 nm, and also demonstrates that from the lower electronic excited state of B800 BChls to the higher exci-tonic state of B850 BChls is an efficient routine for energy transfer. The excitation energy in higher excitonic states of B850 BChls relaxes rapidly to the next lowest excitonic state by inter-conversion, delocalization to adjacent molecular, populating the lowest excitonic state and the change of molecular conformation.

关键词： photosynthesis, light-harvesting complex, energy transfer, ultrafast, dynamics, purple bacteria.

Synthesis and crystal structure of SrNi_xV_(6-x)O_(11)

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chinese Science Bulletin 1997年第10期42卷 850-855页

作者： LIU Hongchao, GUO Changlin and TU Hengyongshanghai institute of Ceramics, chinese academy of sciences, shanghai 200050, china shanghai institute of Ceramics Chinese Academy of Sciences Shanghai China

THE AV6O11（A,alkaline or alkaline earth metals）-type compounds are the only phases

关键词： SrNixV6-xO11 crystal structure X-ray powder diffraction Rietveld method bond-valence method.

Recent progress in neurodegenerative disorder research in china

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Science china(Life sciences) 2010年第3期53卷 348-355页

作者： ZHOU JiaWei institute of Neuroscience,shanghai institutes for Biological {3.,chinese academy of {3.,shanghai,20003. 1. institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Neurodegenerative disorders,including Alzheimer's disease(AD) and Parkinson's disease(PD),are common disorders of the central nervous system among aging *** the last 10 years insights concerning the etiology,diagnosis and pathogenesis of these diseases have come from research carried out by chinese *** findings include the description of chinese patients with autosomal recessive early-onset PD,the function of the tau protein,molecular mechanisms underlying protein aggregation,and the identification of biomarkers for AD diagnosis and molecules/compounds with potential neuroprotective activities.

关键词： neurodegenerative disorders pathogenesis diagnosis neuroprotection Alzheimer’s disease Parkinson’s disease

Influence of Transited Layer of Multilayer on X-ray Diffraction Intensity and Calculation of the Thickness

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chinese Journal of Lasers 2000年第2期9卷 134-139页

作者： FENG Shimeng YI Kui ZHAO Qiang TANG Zaosen FAN Zhengxiou (shanghai institute of Optics and Fine Mechanics, The chinese academy of sciences, shanghai 201800, china) 中科院上海光学精密机械所上海

This paper integrates the roughness with the diffuse layer as a transited layer factor for study of influence of the practical interface on the X-ray diffraction intensity of multilayer. By the study of a simple model of transited layer presented in this paper, it is given a formula to describe the quantitative relation of the transited layer with the X-ray diffraction intensity. The calculated thickness of transited layer of Mo/Si multilayer is given, which is consisted with the values measured with the high resolution microscopy.

关键词： transited layer diffraction intensity multilayer

Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 673-680页

作者： Chun-Hui HOU, Jian HUANG, and Ruo-Lan QIAN* State Key Laboratory of Molecular Biology, institute of Biochemistry and Cell Biology, shanghai institutes for Biological {3., chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 20003., china State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3.28 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3.04 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA