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Comparison study of trapped water in human erythrocytes by EPC and DSC method and its application

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Science china(Technological Sciences) 2004年第3期47卷 343-351页

作者： ZHAO Gang1, HE Liqun1, GUO Xiaojie2, LIU Zhong3. LUO Dawei1,3.& GAO Dayong1,3.1. Cryobiomedical Engineering Research Institute, Department of Thermal Science and Energy Engineering, University of Science and Technology of china, hefei 23.027, china 2. {3. cross blood center of hefei, hefei 230022, china 3. Department of Mechanical Engineering, University of Kentucky, Lexington, KY 40506-0108, USA Univ Sci & Technol china Cryobiomed Engn Res Inst Dept Thermal Sci & Energy Engn Hefei 230027 Peoples R China red cross blood Ctr {3. Hefei 230022 Peoples R China Univ Kentucky Dept Mech Engn Lexington KY 40506 USA

The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53. of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3.86 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 3.%—40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%—3.% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.

关键词： differential scanning calorimeter/DSC, electronic particle counter/EPC, cell volumetric change, red blood cell/RBC/erythrocyte

Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

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Neural Regeneration Research 2006年第7期1卷 618-621页

作者： Yong Man1, Jianbin Li2, Bo Yang3. Ji Ma1 1Department of Cerebral Surgery, the Second People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, china 2red cross {3. center of Henan Province, Zhengzhou 450000, Henan Province, china 3.epartment of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, china Department of Cerebral Surgery The Second People's Hospital of Zhengzhou Zhengzhou 450000 Henan Province China red cross blood {3. of Henan Province Zhengzhou 450000 Henan Province China Department of Neurosurgery Hospital of Zhengzhou University Zhengzhou 450000 Henan Province China

BACKGROUND: Transplanted mononuclear cell (MNC) of umbilical blood can survive in central nervous system (CNS) of host through blood brain barrier, differentiate into nerve cells, migrate to damaged site and integrate morphological structure and function with nerve cells of host so as to improve deficiencies of sensatory function, motor function and cognitive function and influence on stroke sequela. OBJECTIVE: To observe the vein transplantation of human umbilical cord blood stem cells (HUCBSC) for improving neurological function, limb function and activity of daily living of patients with stroke and evaluate the reliability. DESIGN: Self-controlled study. SETTING: Department of Neurosurgery, the Second People's Hospital of Zhengzhou City; {3.-crossed blood center of Henan Province; Department of Neurosurgery, the Fist Affiliated Hospital of Zhengzhou University. PARTICIPANTS: A total of 10 patients with stroke sequela were selected from Department of Cerebral Surgery, the Second People's Hospital of Zhengzhou City from April to December 2005. There were 9 males and 1 female aged from 3. to 75 years with the mean age of 56 years. All of them were diagnosed with CT and MRI examination and coincidence with diagnostic criteria of stroke established by the Fourth National Academic Meeting for Cerebrovascular Disease. All patients provided informed consent. METHODS: 80-140 mL umbilical blood of term birth of newborn was selected hermetically and maintained in sterile plastic bag. And then, the blood was centrifugated at the speed of 1 500 r/min for 3. minutes at 22 ℃ in order to separate MNC, i.e., HUCBSC. In addition, after final diagnosis during hospitalization, stroke patients were perfused with HUCBSC through superficial vein of back of the hand. Each patient was averagely perfused with 6 portions of HUCBSC (cellular numbers ≥ 1×108/portion) and the interval between each portion was 1-7 days with the mean interval of 4 days. MAIN OUTCOME MEASURES: ① Neurological fun

关键词： cell stem Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

Non-freeze-drying injuries of freeze dried living cells

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Non-freeze-drying injuries of freeze dried living cells

第六届全国低温生物医学及器械学术大会

作者： Zhiquan Shu~(1,2),Liu He~1,Dayong Gao~2,Haifeng Zhang~1,Zhong Liu~3.and Liqun He~(*1) 1 Dept.of Thermal Science and Energy Engineering,University of Science and Technology of china,hefei, 23.027,china 2 Department of Mechanical Engineering,University of Washington,Seattle,98195-2600,USA. 3.The {3. cross Transfusion center of hefei,hefei,23.000,china

The understanding of cell injuries in the process of freeze drying is crucial to raise survival rates of living *** reality,the injuries of freeze-dried cells are not all made in the sole process of freeze drying,and some are also made in other processes,including injuries during addition or removal of protective agents as well as injuries caused by *** subdividing the injures into freeze drying ones(only caused in freezing and drying process) and non-freeze-drying ones,the freeze dried damages can be reduced also by eliminating the non-freeze-drying ***,we propose that the non-freeze-drying damages are mainly made by osmotic *** red blood cells as model,we propose some ways to reduce these damages in the non-freeze-drying processes by properly adjusting the osmotic pressure.

关键词： freeze drying red blood cell rehydration trehalose injury

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地市级建立血站的模式思考

天水师范学院学报 1991年第4期11卷 83-85页

作者：乔之宏合肥市红十字中心血站

本文根据国情,用系统方法(System Methool,SM)和功能模拟方法(Function Simulation Methool,FSM)的现代思维方法论的观点提出了快速、节约、高效地建立地市级血站的模式,论证了建立自动运转机制的观点,对建立地市级血站的决策有现实指... 详细信息

本文根据国情,用系统方法(System Methool,SM)和功能模拟方法(Function Simulation Methool,FSM)的现代思维方法论的观点提出了快速、节约、高效地建立地市级血站的模式,论证了建立自动运转机制的观点,对建立地市级血站的决策有现实指导意义,对县血站建立亦具有现实意义.

关键词：血站模式系统方法功能模拟方法

STUDY OF DIELECTRIC PROPERTIES OF HUMANred blood CELL SUSPENSION FROM 243.EALTHY PERSONS AT RADIO FREQUENCIES

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Chinese Journal of Biomedical Engineering(English Edition) 1994年第1期3卷 20-27页

作者： Lu Yongjun,Yu Jue,Ren Yinhua(Dept. BME, Institute of Basic Medical Sciences, Beijing, 100005,china)Zhang Zhixin,Sun Yanmei,Zhi Hong,Yuan Qinzhen(blood center of Beijing {3. cross Society,Beijing, 100086,china)

Dielectric properties or kuamn red blood cell (RBC) suspension (ltematocrit 50%) from 243.healthy persons (120 males, 123.remales) were measured at 25Cin the frequency range rrom 1 to 500MHz, and analysed statistically. The measuringsystem controlled by an IBM-PC computer was composed or a network analyzer(HP4195A), coaxial line sensor and a temperature controlling set. The results showedthat the dielectric properties did not depend on either sex or blood group. However, agehad definite erfect. There was a critical age around 49 years when the dielectric data ofhuman RBC suspensions varied signiricantly.

关键词： Human red blood Cell Suspension Cell Suspension Dielectric properties Heaalthy volunteer Biodielectric Measurement

胶束薄层荧光法同时测定体液中诺氟沙星和氟罗沙星含量的研究

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分析测试学报 2004年第2期23卷 81-83页

作者：周源冯育林太原市红十字血液中心山西太原030024 山西大学现代化学研究所山西太原030006

研究了胶束薄层色谱法测定体液中诺氟沙星(norfloxacin)和氟罗沙星(fleroxacin)含量的方法 ;以聚酰胺层析板为固定相 ,使用0.01mol/L的十二烷基磺酸钠(SDS)水溶液作为展开剂 ,并将EDTA溶液加入到展开剂中避免了氟哌酸 -金属离子络合物... 详细信息

研究了胶束薄层色谱法测定体液中诺氟沙星(norfloxacin)和氟罗沙星(fleroxacin)含量的方法 ;以聚酰胺层析板为固定相 ,使用0.01mol/L的十二烷基磺酸钠(SDS)水溶液作为展开剂 ,并将EDTA溶液加入到展开剂中避免了氟哌酸 -金属离子络合物的生成 ,改善了分离条件 ;在此条件下 ,分离后的NFLX和FLX其Rf值分别为0.62和0.80 ;试样斑点经薄层色谱扫描仪用荧光法扫描定量 ,激发波长为278nm(NFLX)和285nm(FLX) ;两个化合物的线性范围分别是0～90ng/斑点 (NFLX)和0～80ng/斑点 (FLX) ;回归方程为c=4.96×10-3.A -2.94(NFLX)和c=2.98×10-3.A-3.54(FLX) ,相关系数均在0.998以上 ;在血样和尿样中回收率为98%～102% ,RSD<5.2%。

关键词：体液诺氟沙星氟罗沙星含量测定同时测定胶束薄层荧光法十二烷基磺酸钠

Rapid Identification of Pathogenic Bacteria by means of TwoConservative Gene Loci′ Specific PCR-CE-RFLP

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Journal of Microbiology and Immunology 2003年第1期1卷 38-43页

作者：高鹏张卓然徐维家安万新张晓慧戴兵范艳萍王运铎李萍温杰于卫健Dalian red cross blood {3. Dalian 116001 china 高向仪谢凡迪王永海 Dalian Municipal center Hospital Dalian 116033 China Dalian Medical University Dalian 116027China Dalian center of Clinical Laboratory Dalian 116033 China Dalian red cross blood {3. Dalian 116001 China Zhejiang Senior Medical School Zhejiang 310052 China Dalian University Affiliated Hospital Dalian 116021 China Zhuanghe No.1 people's Hospital Zhuanghe 116400 China

To establish a rapid identification method for common pathogenic bacteria on the basis of molecular biology and to construct a preliminary Polymerase Chain Reaction-Capillary Electrophoresis - Restriction Fragment Length Polymorphism (PCR-CE-RFLP) database of bacteria isolated from clinical specimens frequently, 183.strains collected from clinical samples belonging to 12 genera and 19 species whose biochemical characterizations corresponded to the typical ones were examined. The genomic DNAs were amplified by two pairs of fluorescence labeled primers aiming at 16S rRNA gene and 16S-23. rRNA spacer region gene respectively at the same time. PCR products were then digested by restriction endonuclease HaeⅢ incompletely before taking capillary electrophoresis. The results with the PCR-CE-RFLP patterns of 16S rRNA genes were just alike within some genera, but when it comes to 16S-23. rRNA spacer region genes, each bacterium showed a unique pattern, which can be distinguished from each other easily. It seems that PCR-CE-RFLP patterns of 16S rRNA gene could only be used to classify the bacteria into family level, whereas the data of 16S-23. rRNA spacer region gene could be utilized to identify the whole microorganisms as precisely as the species level. In spite of the data of the spacer region gene alone can be sufficiently to verify the whole bacteria, we insist that the 16S rRNA gene could be of some assistant in case that there should be lots of families of bacteria, in which some similar ones, with the same RFLP data of 16S-23. rRNA spacer region gene, may coexist. This study proves that the utility of PCR-CE-RFLP is a convenient, rapid method to identify pathogenic bacteria, and is also a quick diagnosis measure for application to clinical use.

关键词： S rRNA gene 16S-23. rRNA gene spacer region Polymerase chain reaction Pathogenic bacteria, RFLP

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并行文件系统扩容与I/O节点负载平衡策略

计算机工程 2000年第10期26卷 121-123页

作者：曲凤山黄刘生黄刘生吴雪梅李家俊中国科学技术大学计算机系合肥230027 国家高性能计算中心合肥230027 哈尔滨市红十字会中心血站哈尔滨150040 大庆物探公司研究所大庆163357

在大规模并行处理系统ＭＰＰ商业化应用中，并行文件系统容量与Ｉ／Ｏ节点的负载平衡一直是挖掘整个系统并行性，进而提高系统性能的关键问题之一．本文从分析Ｐａｒａｇｏｎ大规模并行处理系统的体系结构和并行文件系统的体系结构出发... 详细信息

在大规模并行处理系统ＭＰＰ商业化应用中，并行文件系统容量与Ｉ／Ｏ节点的负载平衡一直是挖掘整个系统并行性，进而提高系统性能的关键问题之一．本文从分析Ｐａｒａｇｏｎ大规模并行处理系统的体系结构和并行文件系统的体系结构出发，给出了并行文件系统的扩容策略，使用ＭＰＰ系统的Ｉ／Ｏ节点平衡负载理论模型，给出了实际工程应用中的负载平衡策略．

关键词：并行文件系统负载平衡 I/O节点操作系统

人血红蛋白提纯、改造制备人工血液制品的研究

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天津医药 2003年第6期31卷 346-348页

作者：樊晶王学谦天津市血液中心 300110

目的 :利用过期血液 ,有效地提纯血红蛋白 ,制备通用人工血液制品 ,以减少血源浪费。方法 :用萃取_超滤法分离、提纯纯品血红蛋白 (SFHb) ;将磷酸吡哆醛与SFHb结合 ,再经戊二醛交联聚合 ,形成大分子的多聚血红蛋白 (Poly_Hb_P) ;最后经... 详细信息

目的 :利用过期血液 ,有效地提纯血红蛋白 ,制备通用人工血液制品 ,以减少血源浪费。方法 :用萃取_超滤法分离、提纯纯品血红蛋白 (SFHb) ;将磷酸吡哆醛与SFHb结合 ,再经戊二醛交联聚合 ,形成大分子的多聚血红蛋白 (Poly_Hb_P) ;最后经微囊包被多聚血红蛋白(VHb) ,进行人工红细胞制备。同时相应鉴定Poly_Hb_P的品质 ,并与SFHb各项指标进行相应比较。结果 :血红蛋白纯品浓度平均为146g/L(4批次 ) ,纯度为98 3.% ;Poly_Hb_P的浓度平均为52 3./L(4批次) ,纯度为95 2 % ;血型凝集实验阴性 ,无菌、热源实验阴性。Poly_Hb_P的各项结果不同于SFHb ,且携氧能力有所增加。VHb制品具有生物活性。结论 :可利用过期血液制备高纯度。

关键词：人工血液制品制备人血红蛋白提纯血液代用品磷酸吡哆醛