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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science china(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2.3.ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3.&ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing {2. of Agriculture and Forestry Science,Beijing 100097,china 2.Department of Chemistry and Chemical Engineering,Hunan institute of Engineering,Xiangtan 411104,china 3.College of Life Science,Capital Normal University,Beijing 10003.,china 4 College of Life Science,Zhongkai University of Agriculture and Technology,Guangzhou 5102.5,china 5 College of Grassland Science,Gansu Agricultural University,Lanzhou 73.070,china 6 Department of Biology,Henan institute of Science and Technology,Xinxiang 453.03.china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 2.1}000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3.5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 3.0 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3.3.pattern using a GeneTAC?G3.arraying robot after being transferred manually into three 3.4-well *** 2.01 and 2.90 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 3.4-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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Synthesis and Crystal Structure of Titanium Imido Complex

Synthesis and Crystal Structure of Titanium Imido Complex

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第十六届全国金属有机化学学术讨论会

作者： Chen Zhou~1,Chen Yanmei~1,Hu Bin~1,Yahong Li~(1,2.) 1.Qinghai institute of salt {2.,{3. academy of Sciences,Xining,810008,china 2.Key Laboratory of Organic Synthesis of Jiangsu Province,College of Chemistry and Chemical Engineering,Suzhou University, Suzhou,2.512.,china)

Two titanium imido complexes with tridentate-substituted pyrrolyl ligand-Ti(NBu)(CHN)dpy(1) and Ti(NPh)(CHN)py(2.,have been synthesized by addition of py,dpy and HNBu or HNPh with Ti(NMe)(CHN) *** titanium imido compl...

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Study on the Metastable Zone Property of Borax Decahydrate in Different Concentrations of LiCl and Li2.O4 Solutions

Study on the Metastable Zone Property of Borax Decahydrate i...

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第十六届全国化学热力学和热分析学术会议

作者： J.Y.Peng~(1,2.,Y.P.Dong~(2.),L.L.Li~(1,2.,Q.F.Meng~2.W.Li~2.1 Qinghai institute of salt {2.,{3. academy of Sciences,810008,Xining, 2.Graduate University of {3. academy of Sciences,10003.,Beijing.

＜正＞Supersaturation is the driving force for the nucleation and the crystal growth during a crystallization *** extent of the supersaturation is defined as the metastable zone *** knowledge of metastable zone width ... 详细信息

关键词： metastable zone width borax impurities

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Gene expression and molecular characterization of a thermostable trehalose phosphorylase from Thermoanaerobacter tengcongensis

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Science china(Life Sciences) 2005年第3期48卷 221-227页

作者： REN Yuanyuan 1,2., DAI Xiuyu 1 , ZHOU Jian 1 , LIU Jingfang 1 , PEI Huadong 1,2.& XIANG Hua 1 1. State Key Laboratory of Microbial Resources and Center for Molecular Microbiology, institute of Microbiology, chinese {2. of Sciences, Beijing 100080,{3. 2. Graduate School of chinese {2. of Sciences, Beijing 10003.,{3. 1. State Key Laboratory of Microbial Resources and Center for Molecular Microbiology Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China 2. Graduate School of chinese academy of Sciences 100037 Beijing China

A gene encoding the trehalose phosphorylase (TreP), which reversibly catalyzes trehalose degradation and synthesis from α-glucose-1-phosphate (α-Glc-1-P) and glucose, was cloned from Thermoanaerobacter tengcongensis and successfully expressed in Escherichia coli. The overexpressed TreP, with a molecular mass of approximately 90 kDa, was determined by SDS-PAGE. It catalyzes trehalose synthesis and degradation optimally at 70℃ (for 3. min), with the optimum pHs at 6.0 and 7.0, respectively. It is highly thermostable, with a 77% residual ac- tivity after incubation at 50℃ for 7 h. Under the optimum reaction conditions, 50 μg crude en- zyme of the TreP is able to catalyze the synthesis of trehalose up to 11.6 mmol/L from 2. mmol/L α-Glc-1-P and 12. mmol/L glucose within 3. min, while only 1.5 mmol/L out of 2.0 mmol/L tre- halose is degraded within the same time period. Dot blotting revealed that the treP gene in T. tengcongensis was upregulated in response to salt stress but downregulated when trehalose was supplied. Both results indicate that the dominant function of the T. tengcongensis TreP is catalyzing trehalose synthesis but not degradation. Thus it might provide a novel route for indus- trial production of trehalose.

关键词： gene expression, Thermoanaerobacter tengcongensis, trehalose phosphorylase, dot blot.

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工业废碱催化剂MA与煤混合浸渍干燥的研究

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燃料化学学报 2004年第3期32卷 335-339页

作者：旷戈张济宇曾才中国科学院山西煤炭化学研究所山西太原030001 福州大学化学工程技术研究所福建福州350002

以转筒干燥方式考察了福建无烟粉煤与工业废碱催化剂MA同时进行混合浸渍与干燥脱水的过程,讨论了煤的粒径,干燥温度,时间、气速、转筒转速、催化剂含量和转筒处理量等因素对煤与催化剂混合浸渍与干燥过程的影响。研究表明采用转筒干燥... 详细信息

以转筒干燥方式考察了福建无烟粉煤与工业废碱催化剂MA同时进行混合浸渍与干燥脱水的过程,讨论了煤的粒径,干燥温度,时间、气速、转筒转速、催化剂含量和转筒处理量等因素对煤与催化剂混合浸渍与干燥过程的影响。研究表明采用转筒干燥的方式,进口干燥空气温度在80℃～160℃、转筒处理量为2.4kg m3.h～183.kg m3.h、干燥气速0.12. s～0.3.m s、干燥时间2.min内,混合物料的湿含量可降至<5%,且催化剂与煤浸渍充分均匀,满足流化床操作的要求,实现了最佳的催化气化效果。基于实验数据拟合了煤和废碱催化剂转筒干燥的传热系数方程,通过物料与能量衡算,模拟了混合物料连续干燥时沿转筒的湿含量的变化。

关键词：煤气化转筒干燥工业废碱

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PREPARATION OF Pb-Ca-Na MASTER ALLOY USING MOLTEN CHLORIDE salt ELECTROLYSIS

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中国有色金属学会会刊：英文版 1996年第2期6卷 18-19,21+25页

作者： Song Renying Wang Xingjie Luo Shumei Jiang Chan Tang Dingxiang(Changchun institute of Applied Chemistry ,chinese {2. of Sciences, Changchun 13.02.) chinese ACAD SCI CHANGCHUN INST APPL CHEMCHANGCHUN 130022PEOPLES R CHINA

ＰＲＥＰＡＲＡＴＩＯＮＯＦＰｂ－Ｃａ－ＮａＭＡＳＴＥＲＡＬＬＯＹＵＳＩＮＧＭＯＬＴＥＮＣＨＬＯＲＩＤＥＳＡＬＴＥＬＥＣＴＲＯＬＹＳＩＳＳｏｎｇＲｅｎｙｉｎｇ；ＷａｎｇＸｉｎｇｊｉｅ；ＬｕｏＳｈｕｍｅｉ；ＪｉａｎｇＣｈ... 详细信息

ＰＲＥＰＡＲＡＴＩＯＮＯＦＰｂ－Ｃａ－ＮａＭＡＳＴＥＲＡＬＬＯＹＵＳＩＮＧＭＯＬＴＥＮＣＨＬＯＲＩＤＥＳＡＬＴＥＬＥＣＴＲＯＬＹＳＩＳＳｏｎｇＲｅｎｙｉｎｇ；ＷａｎｇＸｉｎｇｊｉｅ；ＬｕｏＳｈｕｍｅｉ；ＪｉａｎｇＣｈａｎ；ＴａｎｇＤｉｎｇｘｉａｎｇ（...

关键词： Pb-Ca-Na master alloy molten chloride salt electrolysis preparation

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Effect of an Alien Species Spartina alterniflora Loisel on Biogeochemical Processes of Intertidal Ecosystem in the Jiangsu Coastal Region,china

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Pedosphere 2008年第1期18卷 77-85页

作者： ZHOU Hong-Xia LIU Jin-E ZHOU Jun QIN Pei Life Science School Nanjing University Nanjing 210093 （China） Kunming institute of Botany Chinese Academy of Sciences Kunming 650204 （China）

Spartina alterniflora Loisel, a species vegetating in intertidal flats along the eastern coast of the United States, was introduced in china almost 3. years ago and has become an urgent topic due to its invasiveness in the coastal zone of china. The impacts of this alien species S. alterniflora on intertidal ecosystem processes in the Jiangsu coastland were investigated by comparing the sediment nutrient availability and trace element concentration characteristics in a mud flat and those of a four-year old Spartina salt marsh that had earlier been a mudflat. At each study site, fifteen plots were sampled in different seasons to determine the sediment characteristics along the tidal flats. The results suggested that Spartina salt marsh sediments had significantly higher total N, available P, and water content, but lower pH and bulk density than mudflat sediments. Sediment salinity, water content, total N, organic C, and available P decreased along a seaward gradient in the Spartina salt marsh and increased with vegetation biomass. Furthermore, the concentrations of trace elements and some metal elements in the sediment were higher under Spartina although these increases were not significant. Also, in the Spartina marsh, some heavy metals were concentrated in the surface layer of the sediment. The Spartina salt marsh in this study was only four years old; therefore, it is suggested that further study of this allen species on a longer time frame in the Jiangsu coastland should be carried out.

关键词： alien species biogeochemical processes intertidal ecosystem salt marsh Spartina alterniflora Loisel

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CONCEPT, DISTRIBUTION LAW AND FORMATION MACHANISM OF INLAND SALINE ALKALINE WETLAND─ ─ Taking Songliao Plain for Example

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chinese Geographical Science 2000年第3期10卷 63-69页

作者： SUN Guang you1, LUO Xin zheng2., YI Fu ke1 , ZHANG Xiao ping1 (1.Changchun institute of Geography, the chinese {2. of Sciences,Changchun 13.02.,P.R.{3. 2.institute of Geography, {2. of Henan Province, Zhengzhou 450052.P.R.{3.)

The paper, taking Songliao Plain for example, studied the concept, distribution law and formation mechanism of inland saline alkaline wetland. The inland saline alkaline wetland is distributed over the inland region of arid or semi arid climate; it is the wet or slightly stagnant environment, forming saline alkaline soil and salt vegetation and making the geogra phical environment fonming complex with an ecosystem of saline alkaline wetland. The laws of zone nature and non zone nature control the distribution of the inland saline alkaline wetland that extensively spreads in north part of china and other countries. The inland saline alkaline wetland is formed jointly by atmosphere, hydrosphere, lithosphere and biosphere including intellectual sphere. Under the special condition, the artificial activity is possibly the key function. In order to improve the human environment, it is a great duty confronting the whole world to rationally transform the inland saline alkaline wetland. Therefore, we presented an anti inland theory to promote the environment transformation.

关键词： Songliao Plain inland saline alkaline wetland wetland distribution law wetland concept wetland formation mechanism

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Production of β-1,3.glucanase and chitinase of two biocon-trol agents and their possible modes of action

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chinese Science Bulletin 2002年第4期47卷 292-296页

作者： FAN Qing, TIAN Shiping, LIU Haibo & XU Yonginstitute of Botany, chinese {2. of Sciences, Beijing 100093.{3. institute of Botany Chinese Academy of Sciences Beijing China

Pichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3.glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3.glucanase activity was detected in Lilly-Barnett minimal {2. medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3.glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2.SU. The lowest β-1,3.glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3.glucanase and chitinase activity was also triggered by wounding, adding of carbon sources

关键词： biological control yeast rhizopus rot β-1 3.glucanase chitinase.

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THE　RELATION　BETWEEN　MOLECULAR　MOTION　AND　ION　CONDUCTIVITY　ON　A　NEW　COMBLIKE　POLYMER

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chinese Chemical Letters 1996年第7期7卷 653-656页

作者： Li QI Yun Qing LIN Fo Song WANG(Changchun institute of Applied Chemistry, chinese {2. of Sciences,Changchun 13.02.) chinese ACAD SCI CHANGCHUN INST APPL CHEMCHANGCHUN 130022PEOPLES R CHINA

A new amorphous comblike polymer(CBP) based on methylvinyl ether/maleic anhydride alternating copolymer backbone and on oligooxyethylene side chain was synthesized The dynamicmechanical properties of CBP-Li salt complexes showed that there were two glass *** are two peaks in the plot of the ionic conductivity *** salt concentration. The plot ofLog σagainst 1/(T-To) shows an unusual dual VTF behavior when using side chain glasstransition temperature (Tβ) as To.

关键词： RELATION THE MOLECULAR AND BETWEEN

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