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Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2.3. Lei Gang1,2. Wuriyanghan Hada1,2. Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant Genomics, institute of Genetics and Developmental Biology, Chinese {2. of sciences, Beijing 100101,{3. 2. Graduate School of Chinese {2. of sciences, Beijing 10003.,{3. 3. Xinjiang {2. of Agricultural and Reclamation science, Shihezi 83.000,{3.) National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School of Chinese academy of sciences Beijing 100039 China Xinjiang academy of Agricultural and Reclamation science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2.and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2.and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

Modeling and verifying Web services driven by requirements:An ontology-based approach

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science in china(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3.JIN ZHi1,2.& WU Budan1,4 1. academy of Mathematics and Systems {2., Chinese academy of {3., Beijing 100080, china 2. institute of Computing Technology, Chinese academy of {3., Beijing 100080, china 3. institute of Software, Chinese academy of {3., Beijing 100080, china 4. Graduate University of Chinese academy of {3., Beijing 100049, china 1. academy of Mathematics and Systems science Chinese Academy of Sciences Beijing 100080 China 3. institute of Software Chinese Academy of Sciences Beijing 100080 China 2. institute of Computing Technology Chinese Academy of Sciences Bejing 100080 China 4. Graduate University of Chinese academy of sciences Beijing 100049 China

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

A high-resolution late Pleistocene record of pollen vegetation and climate change from Jingning, NW china

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science china Earth {2. 2006年第2期49卷 154-162页

作者： LI Chunhai12. TANG Lingyu1, FENG Zhaodong3.4, ZHANG Hucai2. WANG Weiguo3.& AN Chenbang3.1. nanjing institute of Geology and Palaeontology, Chinese academy of {3., nanjing 2.0008, china 2. Nanjng institute of Geography and Limnology, Chinese academy of {3., nanjing 2.0008, china 3. Environment and Resources College, Lanzhou University, Lanzhou 73.000, china 4. Beijing Normal University, Beijing 100875, china 1. nanjing institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China 2. Nanjng institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China 3. Environment and Resources College Lanzhou University Lanzhou 730000 China 4. Beijing Normal University Beijing 100875 China

With the support of 18 AMS-14C data, a high-resolution pollen record from the Suancigou section at Jingning, Gansu Province was established. Our results showed that the vegetation ex-perienced a series of changes characterized by the alternative growth and decline of forest and steppe components during the period from 44.2.to 11 ka B.P. in the Jingning area. Over the period of 44―2. ka B.P. (MIS3., coniferous forests were flourishing, indicating a humid climate with the tem-perature being lower than that of the present. Since 2.0}ka B.P., the vegetation was dominated by steppe-desert steppe under a cold and dry condition, and entered the Last Glacial Maximum domi-nated by desert steppe. Several periods of the marked decrease in the arborous pollen content in Suancigou well corresponded to the North Atlantic Heinrich events, especially to Heinrich event 3. further supporting that the Heinrich events were well reflected by the pollen record in the Loess Pla-teau as well.

关键词： Western Loess Plateau, pollen record, climatic change, late Pleistocene.

Study on growth and properties of novel γ-LiAlO_2.substrate

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science china(Technological {2.) 2006年第2期49卷 188-193页

作者： ZOU Jun1,2. ZHOU Shengming1, XU Jun1 & ZHANG Rong3.1. Shanghai institute of Optics and Fine Mechanics, Chinese {2. of sciences, Shanghai 2.1800,{3. 2. Graduate School of Chinese {2. of sciences, Beijing 10003.,{3. 3. Laboratory of Solid State Microstructures and Department of Physics, nanjing University, nanjing 2.0093.{3. 1. Shanghai institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China 2. Graduate School of Chinese academy of sciences Beijing 100039 China 3. Laboratory of Solid State Microstructures and Department of Physics Nanjing University Nanjing 210093 China

Transparent γ-LiAlO2.single crystal has been grown by Temperature Gradient technique. The surface of the wafer annealed in O2.atmosphere at 1100°C for 70 h became opaque and Li-poor phase (LiAl5O8); while, that annealed in Li-rich atmosphere kept transparent and smooth. The full-width at half maximum value dropped to 3. arcsecs when the wafer was annealed in Li-rich atmosphere. That annealed in O2.atmosphere increased to 78 arcsec. Compared with absorption spectra, we can conclude that the 196 nm absorption peak was caused by Li vacancies and the 73. nm peak was caused by O vacancies.

关键词： γ-LiAlO2 crystal, scanning electron micrograph (SEM), absorption spectra.

Integrated precise orbit determination of Shenzhou IV unmanned spacecraft

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science china(Technological {2.) 2004年第5期47卷 518-525页

作者： L Congmin1,2. GU Yidong3. LIN Baojun3.& GUO Jiong3.1. Center for Space science and Applied Research, Chinese academy of sciences, Beijing 100080,{3. 2. Graduate School of the Chinese academy of sciences, Beijing 10003.,{3. 3. General Establishment of Space science and Application, Chinese academy of sciences, Beijing 100080,{3. Correspondence should be addressed to L Congmin 1. Center for Space science and Applied Research Chinese Academy of Sciences 100080 Beijing China 2. Graduate School of the Chinese academy of sciences 100039 Beijing China 3. General Establishment of Space science and Application Chinese Academy of Sciences 100080 Beijing China

Considering the characteristic orbit of Shenzhou IV spacecraft and the advantages and disadvantages of several types of domestic orbit measurements, a scheme of IPOD is determined, which uses GPS, SLR and USB observation data jointly plus precise ionosphere corrections to GPS observation data. IPOD system and mathematic models of OD are introduced. The results of different joint OD are analyzed using true observation data, and show that radial accuracy is better than 2.m.

关键词： Shenzhou IV unmanned spacecraft integrated precise orbit determination GPS SLR USB.

Bacterial Communities in a Buried Ancient Paddy soil from the Neolithic Age

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Pedosphere 2010年第3期20卷 389-398页

作者： SHEN Wei-Shou YIN Rui LIN Xian-Gui CAO Zhi-Hong State Key Laboratory of soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of SciencesNanjing 210008 (China) College of Chemistry and Environmental science Nanjing Normal University Nanjing 210097 (China) Joint Open Laboratory of soil and Environment Institute of Soil Science Chinese Academy of Sciences and Hong KongBaptist University Nanjing 210008 (China)

An ancient irrigated paddy soil from the Neolithic age was excavated at Chuodunshan Site in the Yangtze River Delta, close to Suzhou, china. The soil organic matter (SOM) content in the prehistoric rice soil is comparable to the average SOM content of present rice soils in this region, but it is about 5 times higher than that in the parent materials. As possible biomarkers to indicate the presence of the prehistoric paddy soil, the bacterial communities were investigated using the techniques of aerobic and anaerobic oligotrophic bacteria enumeration, Biolog analysis, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that in the buried soil layers, the prehistoric paddy soil had the largest number of aerobic and anaerobic oligotrophic bacteria, up to 6.12.and 5.86 log cfu g-1 dry soil, respectively. The prehistoric paddy soil displayed better carbon utilization potential and higher functional diversity compared to the parent materials and a prehistoric loess layer. The Shannon index and richness based on DGGE profiles of bacterial 16S rRNA genes were higher in prehistoric paddy soil than those in the prehistoric loess soil. It might be concluded that the prehistoric irrigated rice cultivation accumulated the SOM in plowed soil layer, and thus increased soil bacterial populations, metabolic activity, functional diversity and genetic diversity. Bacterial communities might be considered as the sensitive indicators of the presence of the prehistoric paddy soil in china's Yangtze River Delta.

关键词： biomarker denaturing gradient gel electrophoresis oligotrophic bacteria paleosols prehistoric paddy soil

A new DNA algorithm to solve graph coloring problem

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Progress in Natural science:Materials International 2007年第6期17卷 733-738页

作者： Jiang Xingpeng1, Li Yin2. Meng Ya3.and Meng Dazhi2.1. National Laboratory of Pattern Recognition,institute of Automation, Chinese {2. of sciences, Beijing 100080,{3. 2. College of Applied sciences, Beijing University of Technology, Beijing 10002.,{3. 3. School of science, Beijing institute of Technology, Beijing 100081,{3.) National Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing 100080 China College of Applied sciences Beijing University of Technology Beijing 100022 China School of science Beijing Institute of Technology Beijing 100081 China

Using a small quantity of DNA molecules and little experimental time to solve complex problems successfully is a goal of DNA computing. Some NP-hard problems have been solved by DNA computing with lower time complexity than conventional computing. However, this advantage often brings higher space complexity and needs a large number of DNA encoding molecules. One example is graph coloring problem. Current DNA algorithms need exponentially increasing DNA encoding strands with the growing of problem size. Here we propose a new DNA algorithm of graph coloring problem based on the proof of four-color theorem. This algorithm has good properties of needing a relatively small number of operations in polynomial time and needing a small number of DNA encoding molecules (we need only 6R DNA encoding molecules if the number of regions in a graph is R).

关键词： DNA computing NP-hard problem graph coloring problem.

A restudy of Oryctocephalus indicus (Reed,1910)

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Progress in Natural science:Materials International 2006年第11期16卷 1177-1182页

作者： ZHAO Yuanlong~1**, YUAN Jinliang~2. PENG Shanchi~ 2.3., YANG Xinglian~1, PENG Jin~1, LIN Jihpai~4, and GUO Qingjun~5(1. College of Resource and Environment of Guizhou University, Guiyang 550003.{2. 2. nanjing institute of Geology and Palaeontology, Chinese {3. of sciences, nanjing 2.0008,{2. 3. State Key Laboratory of Papaeobiology and Stratigraphy, nanjing institute of Geology and Palaeontology, nanjing 2.0008,{2. 4. Department of Geological science, Ohio State University, Columbus, OH 43.10, USA 5. institute of Geochemistry, Chinese {3. of sciences, Guiyang 550002.{2.) College of Resource and Environment of Guizhou University Guiyang 550003 China nanjing institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China State Key Laboratory of Papaeobiology and Stratigraphy Nanjing Institute of Geology and Palaeontology Nanjing 210008 China Department of Geological science Ohio State University Columbus OH 43210 United States institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China

Restudy on morphology of Oryctocephalus indicus (Reed, 1910), based on specimens from Guizhou, china and Nevada USA, suggests that the subspecies regarded previously as Oryctocephalus indicus indicus (Reed, 1910), O. indicus latus Zhao et Yuan 2.02. and O. indicus kobayashi Saito, 193., are synonymous, and Oryctocephalus americanus Sundberg et McCollum, 2.03.is a similar form of O. indicus. Oryctocephalus indicus is rediagnosed as having a glabella that is subconical in outline and tapering forward slightly; a thorax that comprises 12.segments, and a small pygidium that bears 2.3.axial rings with a terminal piece and a postaxial ridge. Oryctocephalus indicus is widely distributed in eastern Guizhou and ranges through a great interval of Kaili Formation. Its first appearance is almost identical with these events of trilobite extinction-recovery occurring at the end of the Early Cambrian, the alternation of acritach assemblages, the change of trace elements (REE) and stable isotopes (carbon) at the Wuliu-Zengjiaya section. As one of the key forms for defining the traditional Lower-Middle Cambrian boundary (the base of Cambrian Series 3.and Stage 5 in the new Cambrian chronstratigraphic standard), O. indicus has received detailed study and has more advantages than other species such as Ovatoryctocara granulata Tchernysheva, 1962.or Arthricocephalus chauveaui Bergeron, 1899.

关键词： Oryctocephalus indicus restudy Kaili Formation Cambrian Guizhou china.

A mixed method for measuring low-frequency acoustic properties of macromolecular materials

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science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 729-739页

作者： LIU Hongwei1,YAO Lei1,3.ZHAO Hong1,3.ZHANG Jichuan2.3.& XUE Zhaohong2.1. National Key Laboratory of Acoustics,institute of Acoustics,Chinese academy of sciences,Beijing 100080,{3. 2. institute of Chemistry,Chinese academy of sciences,Beijing 100080,{3. 3. Graduate University of Chinese academy of sciences,Beijing 100049,{3. National Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences Beijing 100080 China institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Graduate University of Chinese academy of sciences Beijing 100049 China

A mixed method for measuring low-frequency acoustic properties of macro-molecular materials is presented. The dynamic mechanical parameters of materials are first measured by using Dynamic Mechanical Thermal Apparatus(DMTA) at low frequen-cies,usually less than 100 Hz; then based on the Principles of Time-Temperature Super-position (TTS),these parameters are extended to the frequency range that acousticians are concerned about,usually from hundreds to thousands of hertz; finally the extended dynamic mechanical parameters are transformed into acoustic parameters with the help of acoustic measurement and inverse analysis. To test the feasibility and accuracy,we measure a kind of rubber sample in DMTA and acquire the basic acoustic parameters of the sample by using this method. While applying the basic parameters to calculating characteristics of the sample in acoustic pipe,a reasonable agreement of sound absorp-tion coefficients is obtained between the calculations and measurements in the acoustic pipe.

关键词： low-frequency acoustic properties,dynamic mechanical properties,Principles of Time-Temperature Superposition,acoustic pipe,sound absorption coefficient.