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High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2#, Libin DENG1#, and Changqing ZENG1,2* The institute of Genetic and Developmental Biology, the chinese academy of {3., beijing 100101, china 2beijing Genomics institute, the chinese academy of {3., beijing 1013.0, china The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing Genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 24–3. bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3.were genotyped in a sample of 90 individuals, 4792 sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

FOURIER-LEGENDRE PSEUDOSPECTRAL METHOD FOR THE NAVIER-STOKES EQUATIONS

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Journal of Computational Mathematics 2000年第3期18卷 225-238页

作者： Jian Li (LSEC, institute of Computational Mathematics and Scientific/Engineering Computing, academy of Mathematics and System sciences, {3. academy of sciences, beijing, 100080, china) 中科院计算数学与科学工程计算所科学与工程计算国家重点实验室北京 100080

Constructs a Fourier-Legendre pseudospectral scheme for Navier-Stokes equations with semi-periodic boundary condition. Equation of the scheme; Estimation of errors; Numerical results.

关键词： Navier-Stokes equations Fourier-Legendre pseudospectral method error estimation

Analysis and Modeling on the Spatial Structure of Urban Land Use: A Case of Jinan, china

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chinese Journal of Population,resources and Environment 2007年第2期5卷 34-40页

作者： Zhao Huixia1, 3. Zhang Zulu2, Jiang Luguang 1 1. institute of Geographical sciences and Natural resources Research, {3. academy of sciences, beijing 100101, china 2. College of Population, resources and Environment, Shandong Normal University, Jinan Shandong 250014, china 3. Graduate School of the {3. academy of sciences,{13., china institute of Geographical sciences and Natural resources Research Chinese Academy of Sciences Beijing 100101 China Graduate School of the chinese academy of sciences Beijing 100039 China College of Population Resources and Environment Shandong Normal University Jinan Shandong 250014 China

Based on the data of urban land use obtained by remote sensing interpretation from aero images in 1986 and 2004, the spatial structure of Jinan is studied in this paper with the method of Geographic Information System (GIS). According to the proportion of land for residence, industry and commerce, the urban district can be distinguished into three rings. Compared with the traditional theory of the ring structure in Western coun- tries, they have many distinctive characteristics. The main func- tions of the inner ring include residence, business and banking, but the proportion of residential land (more than 50%) is considerably higher than that of the cities in Western countries. The land proportion for residence and industry in the intermediate ring is equal on the whole. The main land of the outside ring is for industrial use. From 1986 to 2004, great changes have taken place on the land proportion of different types of use. In the inner ring, the land for residential and industrial use has shrunk much while the land for commercial use has increased. In the intermediate ring, the changes of the three types of land use were not very much and the temporal variation was relatively stable. On the contrary, the structure in the outside ring varies acutely. Though most of it is also for industrial use, the proportion of residential land has in- creased much. The three rings can also be divided into several sectors respectively, according to the function of land use clusters. New trends and mechanisms of the changes of land use impacting the urban structure were proposed in the end of the paper.

关键词： urban land use ring structure sector structure Jinan

Inertial theory of the mean Hadley circulation under the condition of nonstatic equilibrium

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Science china Earth sciences 2006年第5期49卷 546-553页

作者： CHAO Jiping1 & LIU Fei2,3.1. National Marine Environmental Forecasting Center, beijing 100081, china 2. Key Laboratory Regional Climate-Environment for Temperate East Asia, institute of Atmospheric Physics, {3. academy of sciences, beijing 100029, china 3. Graduate University of {3. academy of sciences, beijing 100049, china 1. National Marine Environmental Forecasting Center Beijing 100081 China 2. Key Laboratory Regional Climate-Environment for Temperate East Asia Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China 3. Graduate University of chinese academy of sciences Beijing 100049 China

Tropical atmosphere is divided into two layers, the layer near the ground is the planet boundary layer, where the motion is linear, if the radiation equilibrium temperature is given at the top of the boundary layer, the vertical motion there can be obtained. It is the non-viscous ideal atmos- pheric layer above the boundary one, where the motion is nonlinear. After getting the conservation laws of angular momentum, potential temperature and potential vortex, the inertial theory of the Hadley circulation driven by the vertical motion of the boundary layer is established, followed by sev- eral examples of its application.

关键词： Hadley circulation, inertial theory, conservation laws of angular momentum, potential temperature and potential vortex.

RADIAL BASIS FUNCTION INTERPOLATION IN SOBOLEV SPACES AND ITS APPLICATIONS

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Journal of Computational Mathematics 2007年第2期25卷 201-210页

作者： Manping Zhang (School of Mathematical sciences,Fudan University,Shanghai 20043.,china institute of Computational Mathematics,{3. academy of sciences,beijing 100080,china School of Mathematical sciences Fudan University Shanghai 200433 China institute of Computational Mathematics Chinese Academy of Sciences Beijing 100080 China

In this paper we study the method of interpolation by radial basis functions and give some error estimates in Sobolev space H^k（Ω）（k 〉 1）. With a special kind of radial basis function, we construct a basis in H^k（Ω） and derive a meshless method for solving elliptic partial differential equations. We also propose a method for computing the global data density.

关键词： Sobolev space Radial basis function Global data density Meshless method

Re-Os(ICP-MS)dating of the massive sulfide ores from the Jinchuan Ni-Cu-PGE deposit

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Science china Earth sciences 2005年第10期48卷 1672-1677页

作者： YANG Gang1,2, DU Andao1, LU Jiren3. QU Wenjun1 & CHEN Jiangfeng2 1. National Research Center of Geoanalysis, beijing 10003., china 2. Laboratory for Earth Material Cycling, School of Earth and Space sciences, University of Science and Technology of china, Hefei 23.026, china 3. institute of mineral Resource, chinese academy of geological sciences, beijing 10003., china National Research Center of Geoanalysis beijing china Laboratory for Earth Material Cycling School of Earth and Space Sciences University of Science and Technology of China Hefei China institute of mineral Resource chinese academy of geological sciences {3. china

Massive sulfide ore from the Jinchuan Ni-Cu-PGE deposit was dated by Re-Os technique using Carius Tube digestion, Os distillation, Re extraction by acetone and ICP-MS measurement. An isochron giving an age of 83. ± 3. Ma, initial 187Os/188Os ratio of 0.279 ± 0.018 (MSWD = 1.7) and γOs = 13. ± 15 is obtained. The Re-Os age obtained agrees, within analytical uncertainties, with previous SHRIMP zircon U-Pb age of 827 ± 8 Ma (n=5, MSWD = 4.3.. The result suggests that the deposit was formed at Neoproterozoic and that the formation of the Jinchuan Ni-Cu-PGE sulfide deposit may be closely related to the plume activity, which were attributed to the breakup of the Rodinia supercontinent. The high γOs of the massive ores sug-gests that crustal material was involved in the formation of the Jinchuan deposit, however, the quantity of the added crustal material is less than 10%.

关键词： Jinchuan Ni-Cu-PGE deposit, Re-Os dating, ICP-MS.

The Discovery of Phlogopite Exsolution Lamellae in Garnets of Eclogite Inclusions, Liaoning Province

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Acta Geologica Sinica(English Edition) 1997年第1期71卷 33-42页

作者： Zhou Xiuzhong institute of mineral Deposits, chinese academy of geological sciences, {3. Liu Xinzhu institute of mineral Deposits Chinese Academy of Geological Sciences Beijing [?]Zhou Xiuzhong Born in May 193. graduated from Specialty of Geology and Prospecting of Metallic and Nonmetallic minerals Beijing College of Geology in 1964. She is now Associated Research Professor in the Institute of Mineral Deposits CAGs and has long been engaged in researches on mineralogy and petrology.

In No. 50 kimberlite pipe of Fuxian County, Liaoning Province, an eclogite inclusion(nodule), which is extremely rare in kimberlites, was discovered and phlogopite exsolutionlamellae were found in garnets of the inclusion. Microscopic, TEM and energy spectral observa-tions and studies confirmed that these lamellae are phlogopite. They are colourless and acicularin section, generally 0.5-5μm in width and 10-100μm in length. Nevertheless, fine lamellae,0.05-0.1μm wide and 1-2μm long, are also well developed. Along [111] of the garnet, three setsof phlogopite lamellae show oriented arrangement approximately at angles of 60°-70°, indi-cating that these lamellae might be the product of exsolution from garnet as a result ofpressure-release when eclogite ascended from the relatively deep level to the relatively shallowlevel of the mantle. Tiny acicular exsolution minerals (or inclusions) are commonly found ingarnet and pyroxene in eclogite inclusions of kimberlites all over the world and it has been re-ported that the identified exsolution minerals include pyroxene and rutile. This is the first timethat phlogopite exsolution lamillae were found in eclogite inclusions in the world.

关键词： eclogite garnet phlogopite exsolution lamellae Liaoning Province

“Tali Glaciation” on Massif Diancang

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Science china Earth sciences 2007年第11期50卷 1685-1692页

作者： YANG JianQiang1, CUI ZhiJiu2, YI ChaoLu1, SUN JiMin3.& YANG LiRong3.1 institute of Tibetan Plateau Research, chinese academy of {3., beijing 100085, china 2 College of Environmental {3., Peking University, beijing 100871, china 3.institute of Geology and Geophysics, chinese academy of {3., beijing 100029, china 1. institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China 2. College of Environmental sciences Peking University Beijing 100871 China 3. institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

The term of ''Tali Glaciation'' is nominated from Massif Diancang in Yunnan Province. The confusing process of the term''s being put forward is confirmed through literature checking. Based on several times of field trip, the glacial landforms in this region are studied in detail, and the magnitude of the glaciation was determined. According to the numerical ages from TL, AIMS ^(14)C and OSL dating, the earliest glacial advance on Massif Diancang occurred at 3.—40 ka BP, followed by the advances at the last stage of the last glaciation, the late glacial and neoglations in turn, until glaciers vanished at 1.2—1.5 ca. a BP in this region. It can be concluded that glaciers were limited above 3.00 m a.s.l., and no glaciers existed on the lower part and adjacent mountains since the last glaciation.

关键词： Massif Diancang,Tali Glaciation,glacial landform,glaciation sequence,numerical dating

Zonal propagation of kinetic energy and convection in the South china Sea and Indian monsoon regions in boreal summer

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Science china Earth sciences 2004年第12期47卷 1076-1084页

作者： CHEN Longxun 1 ,GAO Hui 2 ,HE Jinhai 2 ,TAO Shiyan 3.& JIN Zuhui 3.1.chinese academy of Meteorological sciences,{3. 100081,china 2.Nanjing institute of Meteorology,Nanjing 210044,china 3.institute of Atmospheric Physics,chinese academy of sciences,{3. 100029,china 1. chinese academy of Meteorological sciences 100081 Beijing China 2. Nanjing institute of Meteorology 210044 Nanjing China 3. chinese academy of sciences Institute of Atmospheric Physics 100029 Beijing China

Zonal propagation of kinetic energy (KE) and convection in the South china Sea (SCS) and Indian summer monsoon areas are examined in present study. Results suggest that the SCS and Indian summer monsoon prevailed regions (5 —15°N) are dominated by the southwesterly wind, however, the disturbances of KE at 850 hPa and convection are observed mainly coming from the western Pacific Ocean (140—150°E), after passing through the SCS, and westward propagated into the Bay of Bengal (90—100°E). In the Indian summer monsoon domain, where the disturbances of KE are found mainly coming from the Arabian Sea (AS) and eastward propagated into the Bay of Bengal. Therefore, the SCS and the Indian summer mon- soon are quite different in zonal propagation of KE and convection. The SCS summer monsoon is mainly affected by the KE and convection coming from the tropical western Pacific. The Indian summer monsoon, however, can be partly influenced by the AS and the SCS summer monsoon. The analysis also suggests that the interaction region between the SCS and the Indian summer monsoon is around 90—95°E, rather than 105°E as proposed by earlier studies.

关键词： zonal propagation of KE and convection, East Asian monsoon, Indian monsoon.