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Science china(Physics,Mechanics & Astronomy) 2014年第4期57卷 615-618页

作者： HE LiPing LIU Shuang DAI JunBeijing National Laboratory for Condensed Matter Physics institute of Physics chinese Academy of sciences Lü HuiBin JIN KuiJuan YANG GuoZhen Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences

Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample ***-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the obliqueincidence reflectivity difference(OIRD)*** obtained the reaction results and the reaction dynamic curves of 500 protein *** addition,we also used label-free detection of protein microarrays of 10080 sample dots,including BSA and different concentrations of Mouse IgG and Rabbit IgG,by *** obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported *** results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.

关键词： oblique-incidence reflectivity difference(OIRD) label-free real-time high-throughput

M@B_9 and M@B_(10) molecular wheels containing planar nona-and deca-coordinate heavy group 11, 12, and 13.metals (M=Ag, Au, Cd, Hg, In, Tl)

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Science china Chemistry 2009年第7期52卷 900-904页

作者： MIAO ChangQing, GUO JinChang & LI SiDian institute of Materials sciences and Department of Chemistry, Xinzhou Teachers’ University, Xinzhou 03.000, china institute of Materials sciences and Department of Chemistry Xinzhou Teachers’ University Xinzhou 034000 China

A density functional and ab initio theory investigation on M@B9 and M@B10 molecular wheels containing planar nona-and deca-coordinate heavy group 11, 12 and 13.metals (M=Ag, Au, Cd, Hg, In, Ti) has been performed. These unusual clusters all prove to be true minima on the potential energy surfaces of the systems and σ+π double aromatic in nature. The first two vertical one-electron detachment energies of M@B-10 (M=Ag, Au) anions and first two ionization potentials of M@B9 (M=Ag, Au) and M@B10 (M=Cd, Hg) neutrals were calculated to aid future experiments. The cluster complexes designed in this work may expend the domain of planar hyper-coordinate elements to include heavy group 11, 12, and 13.metals and serve as interesting candidates to be targeted in experiments.

关键词： planar hyper-coordinate heavy metals density functional theory ab initio geometries electronic structures

Plasma Arc Surface Hardening Robot Technology

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厦门大学学报（自然科学版） 2002年第S1期41卷 171-页

作者： ZHAO Tie-jun 1,2,3. ZHAO Ming-yang 1, ZHAO Wen-zhen 2 (1.Shenyang institute of Automation of the chinese Academy of sciences, Shenyang 110016, {3. 2. Shenyang University of Technology, Shenyang 110023. {3. 3.Graduate School of the chinese Academy of sciences, Beijing 10003., {3.)

In national economy and national defense, a lot of ma chine components become worthless early because of wear and tear and corrode. It leads to huge loss of resource and material. Surface hardening of the steel cou ld form a hard, wearable, corrode-resisting layer on the surface to enhance the mechanical property of the machine component. From 1980s, there is a new method of surface hardening that is heating with plasma arc. It overcomes the shortage of old methods and is adopted in automotive product industry. The plasma equipm ent is small and easy to be controlled, so it could be installed on a robot and moved everywhere. This paper analyzed the character of the plasma at first, then studied the plasm a arc as Guess distribution circular heat source to create the surface hardening temperature field and heating process. The method of the plasma arc surface har dening technology is given. This paper describes hardware design of the plasma arc surface-hardening robot and analyzes its kinematics. This robot is a humanoid form robot with a mobile p latform, a 7 DOF redundancy arm and a 2 DOF trunk. The footprint is an important feature for the robot that has to move in a cluttered environment. Thus the mob ile platform of the plasma arc robot is an omni-directional mobile robot with o rthogonal-wheel assemblies. The trunk is important for keep balance of the robo t. Thus a series spring is fixed in the trunk to provide accurate force feedback at each direction and insulate the shock loads from arm and mobile platform. Th e redundancy arm configuration is similar to a broadly simplified model of the h uman arm with 7 DOF. The maximum radius is about 1 m with a maximum load of abou t 1 kg. The redundancy of the 7 DOF allows the handling of situation in whic h additional movement constraints have to meet and avoids static or dynamic obst acles. It has better adaptation to typical human environment and to allow for hu man-like behavioral strategies in solving complex task

关键词： plasma arc surface hardening robot

Wrinkled petals and stamens 1,is required for the morphogenesis of petals and stamens in Lotus japonicus

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Cell Research 2006年第5期16卷 499-506页

作者： Jiang Hua Chen~1 Ji Liang Pang~2 Li Lin Wang~2 Yong Hai Luo~1 Xin Li~3.Xiang Ling Cao~1 Kui Lin~3.Wei Ma~3.Xiao He Hu~1 Da Luo~(1,3. ~1National Laboratory of Plant Molecular Genetics,institute of Plant Physiology and Ecology,Shanghai institutes for Biological sciences,and Graduate School of the {3. Academy of sciences,{3. Academy of sciences,3.0 Fenglin Road,Shanghai 20003.,china ~2School of Life Science,Hangzhou Normal College,16 Xuelin Road,Hangzhou 3.0018,china ~3.chool of Life Science and Biotechnology,Shanghai Jiao Tong University,1954 Huashan Road,Shanghai 20003.,china NationalLaboratoryofPlantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesandGraduateSchooloftheChineseAcademyofSciencesChineseAcademyofSciences300FenglinRoad

Although much progress has been made in understanding how floral organ identity is determined during the floraldevelopment,less is known about how floral organ is elaborated in the late floral developmental *** we de-scribe a novel floral mutant,wrinkledt petals and stamensl (wps1),which shows defects in the development of petalsand *** analysis indicates that wps1 mutant is corresponding to a single recessive locus at the long arm ofchromosome *** early development of floral organs in wps1 mutant is similar to that in wild type,and the malfunctionof the mutant commences in late developmental stages,displaying a defect on the appearance of petals and *** mature flower,petals and stamen filaments in the mutant are wrinkled or folded,and the cellular morphology underL1 layer of petals and stamen filaments is *** is found that the expression patterns of floral organ identity genesare not affected in wps1 mutants compared with that of wild type,consistent with the unaltered development of allfloral ***,the identities of epidermal cells in different type of petals are *** histologicalanalysis shows that in wps1 flowers all petals are irregularly folded,and there are knotted structures in the petals,whilethe shape and arrangement of inner cells are malformed and *** on these results,we propose that Wps1acts downstream to the class B floral organ identity genes,and functions to modulate the cellular differentiation duringthe late flower developmental stages.

关键词： Lotus japonicus wps1 mutant ABC model floral organ differentiation

Study on Wood Bending Technology

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chinese Forestry Science and Technology 2008年第2期7卷 15-22页

作者： LIU Zhijia1 LI Li2 BAO Fucheng1 WANG Ruijuan3.1. Research institute of Wood Industry, chinese Academy of Forestry, Beijing 100091, P. R. china 2. Academy of Material Science and Technology, Beijing Forestry University, Beijing 100083. P. R. china 3. Beijing Woodworking Factory Co., Ltd, Beijing 100068, P. R. china

Choosing optimum softening process and exploring the factors affecting dimensional stability and their changing laws can be able to provide technological support for development of curving furniture. The total rubber wood samples were 112 [220 mm (longitudinal) × 17 mm (radial) × 8 mm (tangential)], and they were softened by boiling water and bent through using curved molds in the test. The optimum softening process was selected according to MOR value of the samples. Some affecting factors which influenced the dimensional stability of curving samples were investigated in the optimum softening process, including aging time, final moisture content of the samples, treatment time at a temperature of 100 ℃ and soaking time in water. The results indicate that the optimum softening process is as follows: softening temperature 112 ℃, saturation vapor pressure 0.16 MPa and softening time 3.h. All factors would affect the dimensional stability of curving samples, but the influence of soaking time in water was the most remarkable. When the aging time was 2 h and the final moisture content was 9.8%, the dimensional stability of the curving samples was the best. The chord change of the samples was great in the first 2 hours during heat treating process at a temperature of 100 ℃.

关键词： wood bending components softening process dimensional stability affecting factor

^(13.C NMR study of D-pantothenic acid complexes with heavy lanthanide ions in aqueous solution

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chinese Journal of Chemistry 1991年第3期9卷 212-221页

作者： REN,Ji-Min PEI,Feng-Kui WANG,Wen-Yun Changchun institute of Applied Chemistry,chinese Academy of sciences,Changchun 13.022 Changchun institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022

The ^(13.C NMR study of the interaction between the trivalent lanthanide ions(from Dy to Lu) and D-pantothenic acid in aqueous solution was *** unambiguous resonance assignments were made on the basis of pH dependence and the analysis of molecular geometry.^(13.C paramagnetic shifts were separated into the contact and dipolar components by Reilley's *** complex stability constant is 12.0 L/mol for the 1:1 ytterbium *** analysis of the shift data shows that heavy lanthanide ions form isostructural complexes with the substrate and the hyperfine coupl- ing constant is independent of the ions used in the study.D-pantothenic acid is coordinated to the metal via the two oxygens of the carboxyl group with 2.25 of Ln—O *** electron spin density distribution and the structural details of the complex were elucidated from the shift data. It is stated that contact contributions,while small for nuclei of several bonds away from the metal, should not be dismissed a priori.

关键词： than NMR acid

Measurement of ionization distributions at nanometre level in tissue-equivalent gas

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Nuclear Science and Techniques 1998年第2期9卷 92-94页

作者： Dang Bing-Rong Wei Zeng-Quan Li Wen-Jian Li Qiang and Zhang Shu-Min (institute of Modern Phusics, the chinese Academy of sciences, Lanzhou 73.000 ) institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China

The developed track detector consists of two parts: a small drift region to obtain a good tracking and a relatively large multiplication region for getting an enough high gain at low pressure. All the track detector can be moved in the direction perpendicular to the beam by means of a micrometric screw. An automatic system allows the counting gas to flow continuously at constant pressure through the detector during the measurements. The system can measure ionization distributions at a few nanometer range.

关键词：放射生物学离子化粒子分布纳米级

Application of EGFP-EGF fusions to explore mechanism of endocytosis of epidermal growth factor

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Acta Pharmacologica Sinica 2007年第1期28卷 111-117页

作者： Hua JIANG~2 Jie ZHANG~(2,3. Bi-zhi SHI~(2,3. Yu-hong XU~3.Zong-hai LI~(2,3.4) Jian-ren GU~(2,3.4)2 State Key Laboratory of Oncogenes and Related Genes,Shanghai Cancer institute,Medical College of Fudan University 3.School of Pharmacy,Shanghai Jiaotong University,Shanghai 20003.,china StateKeyLaboratoryofOncogenesandRelatedGenes ShanghaiCancerInstituteMedicalCollegeofFudanUniversity SchoolofPharmacy ShanghaiJiaotongUniversityShanghai200032China

Aim:To develop a simple method for monitoring protein localization of epidermalgrowth factor(EGF)in living ***:Enhanced green fluorescent protein(EGFP)was used as an autofluorescent tag to label EGF ***-PAGE andWestern blot analysis were used to detect the expression of the EGFP-tagged EGF(EGFP-EGF)*** cell-binding and internalization activity of EGFP-EGFwere analyzed by fluorescence-activated cell sorting(FACS)and confocal ***:EGFP-EGF protein was expressed in Escherichia coli and purified.A cell-binding assay demonstrated that the EGFP-EGF protein could bind effi-ciently to the cells expressing *** binding and internalization of EGFP-EGFcan be visualized even at a very low concentration under confocal *** FACS-based assay for internalization activity indicated the accumulation ofinternalized EGFP-EGF over ***,the results of the competitionassay indicated its EGFR binding *** such a method,it does notneed to label EGF with chemicals and avoid light in the experimental ***:The fusion protein EGFP-EGF has several characters including highsensitivity,stability and convenience for manipulation,and is a powerful tool forthe study of EGF endocytosis.

关键词： receptor epidermal growth factor endocytosis purification expression

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模糊影响图评价算法的探讨

系统工程学报 2004年第2期19卷 177-182页

作者：程铁信王平张伟波中国科学院系统科学研究所北京100080 加拿大阿尔伯塔大学土木与环境工程系中国华电集团公司北京100035

模糊影响图是进行风险分析与评价的一种较新的方法,因其直观性和易于理解而受到欢迎,论文就可用于工程项目风险分析的模糊影响图评价算法中存在的问题进行了探讨,研究并改正了其评价算法,最后,通过一个算例,详细说明了改正后的评价算法.

关键词：模糊影响图风险分析风险评价算法模糊理论模糊集工程项目

Diagnostic analysis of mesoscale rainstorms in the Jiang-Huai valley of china with convective vorticity vector

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Progress in Natural Science:Materials International 2007年第5期17卷 559-568页

作者： Cui Xiaopeng, Gao Shouting and Li Xiaofan (Laboratory of Cloud-Precipitation Physics and Severe Storms (LACS), institute of Atmospheric Physics, chinese Academy of sciences, Beijing 100029, {3. Joint Center for Satellite Data Assimilation and NOAA/NESDIS/Center for Satellite Applications and Research, Camp Springs, Maryland, USA) [a]Laboratory of Cloud-Precipitation Physics and Severe Storms (LACS) Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China [b]Joint Center for Satellite Data Assimilation and NOAA/NESDIS/Center for Satellite Applications and ResearchCamp Springs Maryland USA

By the vector product of absolute vorticity vectors and equivalent potential temperature gradients, a new vector, convec-tive vorticity vector (CVV) is readdressed after Gao et al., and is applied to the study of the development and movement of mesoscale rainstorms in the Jiang-Huai valley of china by using high-resolution simulation data with PSU/NCAR MM5 briefly. The results show that the CVV, especially its vertical component which is closely related to interactions between vertical secondary circulations and horizontal equivalent potential temperature gradients, may be a good tracer and diagnostic tool in the study of mesoscale rainstorms in the Jiang-Huai valley of china. And the main physical processes responsible for the variations of the vertical component of the CVV are discussed briefly based on its tendency equation proposed in this article.

关键词： convective vorticity vector mesoscale rainstorms tendency equations.