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Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis

Cell Research 1995年第1期5卷 9-24页

作者： GU GUOYAN (FORMERLY KU KUOYEN) CHENGTANG XU KONGHUA ZHANG QIRONG GAO.(Shanghai institute of Cell Biology, chinese academy of sciences, Shanghai 20003., china) Shanghai institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.&#x0002A Dedicated to Prefessor Zhen YAO on the occasion of his 80th birthday.† This work was supported by the director's foundation of Shanghai institute of Cell BiologyReceived 27October1994 Revised 6April1995 Accepted 25April1995

By laser scanning fluorescence microscopy for quan-titative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocyanate duxing cleavage furrow extending forward, it was found that in area of presumptive cleavage furrow the scanning curve became ∨ shape, indicating dark stripe appeared in that place. Then the fluorescence intensity increased at the place where the botton of ∨ shape had located, and the scanning curve tuxned to ∧ shape, indicating single stripe was formed. While enhanced fluorescence appeared on the borders of ∧ shape, an M shape curve was found, show-ing double stripe occurred. During the distance between two borders of M shape incresing from 50 μm to 100μm,a fluorescence peak came to sight in the middle of the M shape, which being the cleavge furrow bottom. The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane. At that time the curve became W shape. By the sides of cleavage furrow the the stress folds became conspicous after double stripe stage, showing the stretching of the egg surface being increased. With our[3., 3.] and others[3.] reports that polylysine could induce the appearance of nascent membrane and phyto-hemagglutinins could decrease or prevent the appearance of nascent membrane, we believed the idea of Schroeder[25] that increasing mechanical stress could initiate nascent membrane formation and thought that the stress lay to the outsides of cleavage furrow.

关键词： cleavage egg surface movement initiating nascent membrane formation laser scanning fluorescence microscopy Rana Amurensis

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The Chiralities of Three Filaments in AR7500

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天文研究与技术 1999年第S1期 433-437页

作者： Jiang Yunchun 1,2,3., Luan Di 1,2 , Lin Ganghua 1,3., Wang Jingxiu 1,3.1 (National Astronomical Observatories, The chinese academy of sciences, Beijing 100012, china) 2 (Yunnan Observatory, The chinese academy of sciences, Kunming 65001

An analysis of structure and evolution of three filaments in AR7500 ON May, 1993.is presented. The three filaments show the following remarkable peculiarities: 1) a sinistral filament lies between two dextral filaments and meets them at two cusps. One of the cusps is rooted in positive polarity plage region while the other in a small decaying spot with negative polarity. 2) Both chromospheric plagette associated fibrils near the filaments and the transverse magnetic fields of the photosphere beneath the filaments almost parallel to the long axis of these filaments, suggesting the dominance of axial field of filaments; the spots near filament’s ends, however, do not show clearly counterclockwise or clockwise superpenumbral fibril pattern. 3. The three filaments do not merge into a single filament during 8 day observation. This can be explained by considering that the magnetic fields along their long axes are in different directions at two cusps. When one of dextral filaments and the sinistral filarment eventually dissipate into fibrils connecting the opposite polarity regions, their jointing cusps can be still clearly observed due to their opposite chiralities.

关键词： Sun: filament Sun:magnetic field

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Conceptualizing and Measuring Economic Resilience of Resource-based Cities： Case Study of Northeast china

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chinese Geographical Science 2017年第3期27卷 471-481页

作者： TAN Juntao ZHANG Pingyu LO Kevin LI Jing LIU Shiwei Northeast institute of Geography and Agroecology Chinese Academy of Sciences Jilin Changchun 130102 China University ofchinese academy of sciences Beijing 100049 China Department of Geography Hong Kong Baptist University Hong Kong China

This paper develops a conceptual model and an indicator system for measuring economic resilience of resource-based cities based on the theory of evolutionary resilience and the related concepts of persistence, adaptation, and transformation. Nineteen resource-based cities in Northeast china were analyzed using the indicator system. The results showed that Liaoning and Jilin provinces had higher economic resilience than Heilongjiang Province. Panjin, Benxi, and Anshan in Liaoning Province were the top three cities, while Shuangyashan and other coal-based cities in Heilongjiang Province ranked last. Metals-and petroleum-based cities had significantly higher resilience than coal-based cities. The differences in persistence, adaptability, transformation, and resilience among resource-based cities decreased since the introduction of the Northeast Revitalization Strategy in 2003. Forestry-based cities improved the most in terms of resilience, followed by metals-based and multiple-resource cities; however, resilience dropped for coal-based cities, and petroleum-based cities falling the most. The findings illustrate the importance and the way to develop a differentiated approach to improve resilience among resource-based cities.

关键词： resource-based cities economic resilience Northeast china

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Relationship between Structure and SHG Effect of Substituted Thienyl Chalcone

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chinese Journal of Structural Chemistry 1997年第4期16卷 263-266页

作者： SU Gen-Bo HE You-Ping LI Zheng-Dong(Fujian institute of Research on the Structure of Matter,the chinese academy of sciences, Fuzhou 3.0002) 中科院福建物质结构所福建福州

The SHG effects of substituted 3.thienyl chalcone compounds weremeasured. The relationship between structure and effect of substituted 3.thienyl chal-cone derivatives and substituted 2-thienyl chalcone derivatives were discussed. It isshown that their properties of high SHG efficient are similar to each other and the sub-stituted 3.thienyl chalcone molecules are easier to form non-centrosymmetric crystalstructure than substituted 2-thienyl chalcone molecules.

关键词： crystal structure SHG effect thienyl chalcone

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A New Triterpenoid from Teucrium Integrifolium

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chinese Chemical Letters 1998年第8期9卷 737-738页

作者： Xing Liang CHEN Tian En WANG Bei JIANG Zhong Wen LIN Han Dong SUN(G.i.h.. academy of Science, Guiyang 550001)(Department of Chemistry, Guizhou University, Guiyang 550025)(Laboratory of Phytochemistry, Kunming institute of botany,{3. academy of Sc Guizhou Acad Sci Guiyang 550001 Peoples R China Guizhou Univ Dept Chem Guiyang 550025 Peoples R China chinese Acad Sci Kunming Inst Bot Lab Phytochem Kunming 650204 Peoples R China

A new ursane-type triterpene, 3.beta-hydroxy-urs-5(6)-en-28-oic acid 1, was isolated from the aerial parts of Teucrium integrifolium and characterized. The structure of this new compound was mainly established by 2D N... 详细信息

关键词： Teucrium integrifolium labiatae ursane-type triterpenoid 3.beta-hydroxy-urs-5(6)-en-28-oic acid

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The Tethyan-Himalayan Orogenic Complex

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Continental Dynamics 1996年第1期 20-29页

作者： Cui Junwen and Xu Zhiqin(institute of Geology chinese academy of Geological {3., Beijing 10003.,china)

ＴｈｅＴｅｔｈｙａｎ－ＨｉｍａｌａｙａｎＯｒｏｇｅｎｉｃＣｏｍｐｌｅｘ￥ＣｕｉＪｕｎｗｅｎａｎｄＸｕＺｈｉｑｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＧｅｏｌｏｇｙＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＧｅｏｌｏｇｉｃａｌＳｃｉｅｎｃｅ... 详细信息

ＴｈｅＴｅｔｈｙａｎ－ＨｉｍａｌａｙａｎＯｒｏｇｅｎｉｃＣｏｍｐｌｅｘ￥ＣｕｉＪｕｎｗｅｎａｎｄＸｕＺｈｉｑｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＧｅｏｌｏｇｙＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＧｅｏｌｏｇｉｃａｌＳｃｉｅｎｃｅｓ，Ｂｅｉｊｉｎｇ１０００３７，Ｃ...

关键词： Himalayan

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Making High Selective Molecule Imprinting Polymer (MIP) In Polar Solvent

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chinese Chemical Letters 1999年第1期10卷 69-72页

作者： Zi Hut MENG Liang Mo ZHOU Jing Fang WANG Qing Hat WANG Dao Qian ZHU(chinese academy of sciences. Dalian {3. of Chemical Physics. 116012) chinese Acad Sci Dalian Inst Chem Phys Dalian 116012 Peoples R China

Acrylamide was used as functional monomer to make high chiral selective molecule imprinting polymers against N-alpha-protected amino acid in polar solvent. The factor, which influence the efficiency of the polymer and... 详细信息

关键词： molecule imprinting chiral separation stationary phase amino acid

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DAPHNIPHYLLUM ALKALOIDS

DAPHNIPHYLLUM ALKALOIDS

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中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Haifeng Wu~(1,*),Xiaopo Zhang~1,Guoxu Ma~1,Lisheng Ding~2,Shilin Chen~(1,3.,Xudong Xu~(1,*) 1 institute of Medicinal Plant Development,chinese academy of Medical {3. and Peking Union Medical College,Beijing 100193.china 2 Chengdu institute of Biology,chinese academy of {3.,Chengdu 610041,china 3.institute of Chinses Materia Medica,china academy of chinese Medical {3.,Beijing 100700, china

The unique polycyclic fused ring systems of Daphniphyllum alkaloids,along with their extensive bioactivities,make this family of alkaloids especially attractive targets for total synthesis and biogenetic *** particular,successive discoveries of new alkaloids with unprecedented skeletons in recent years have made great contribution to structural diversities of alkaloids elaborated by plants of the genus *** the end of 2008,more than 200 alkaloids belonging to 14 different skeletal types have been isolated from different parts of plants of thirteen Daphniphyllum *** alkaloids show cytotoxic,antioxidant,vasorelaxant and anti-platelet activating factor(PAF)*** plausible biosynthetic pathways for Daphniphyllum alkaloids have been proposed and biomimetic total syntheses of some alkaloids *** provide an update of the previous reviews published in 2009,chemical structures,biosynthesis,syntheses and pharmacological activities of Daphniphyllum alkaloids reported in the latest years are presented in this *** 2009 and 2012,additional fifty-one novel alkaloids have been isolated and *** them,some possess unprecedented *** state-of-the-art total syntheses through cascade reactions were completed.

关键词： alkaloids Daphniphyllum biosynthesis total synthesis pharmacological activities

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Delay of ZGA initiation occurred in 2-cell blocked mouse embryos

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Cell Research 2003年第3期13卷 179-185页

作者： JIA JING QIU,WU WEN ZHANG,ZHI Li WU,YI HONG WANG,MIN QIAN,YI PING LIinstitute,of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences.{3.,20003. Shanghai,chinaSchool of Life Science,East china Normal University. 200062 Shanghai,china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 200031 Shanghai China

One-cell mouse embryos from KM strain and B6C3.1 strain were cultured in M16 medium,in which2-cell block generally *** of KM strain exhibited 2-cell block,whereas B6C3.1 embryos,which are regarded as a nonblocking strain,proceeded to the 4-cell stage in our culture *** is oftenassumed that the block of early development is due to the failure of zygotic gene activation（ZGA）in *** this study we examined protein synthesis patterns by two-dimensional gel electrophoresis of[3.S]methionine radiolabeled 2-cell *** from the blocking strain and the nonblocking strainwere compared in their development both in vitro and in *** detection of TRC expression,a markerof ZGA,at 42 h post hCG in KM embryos developed in vitro suggested that ZGA was also initiated even inthe 2-cell arrested ***,a significant delay of ZGA was observed in KM strain as comparedwith normally developed B6C3.1 *** the very beginning of major ZGA as early as 3. h post

关键词： monse embryo two-cell block zygotic gone activation (ZGA).

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New selenoproteins identified in silico from the genome of Anopheles gambiae

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Science china(Life sciences) 2007年第2期50卷 251-257页

作者： JIANG Liang1,2,LIU Qiong1,CHEN Ping3.GAO ZhongHong3.& XU HuiBi3.1 College of Life Science,Shenzhen University,Shenzhen 518060,china 2 Changchun institute of Applied Chemistry,chinese {3. of sciences,Changchun 13.022,china 3.Department of Chemistry,Huazhong University of Science and Technology,Wuhan 43.074,china 1. College of Life Science Shenzhen University Shenzhen 518060 China 2. Changchun institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China 3. Department of Chemistry Huazhong University of Science and Technology Wuhan 430074 China

Selenoprotein is biosynthesized by the incorporation of selenocysteine into proteins,where the TGA codon in the open reading frame does not act as a stop signal but is translated into *** dual functions of TGA result in mis-annotation or lack of selenoproteins in the sequenced genomes of many *** computational tools fail to correctly predict ***,we devel-oped a new method to identify selenoproteins from the genome of Anopheles gambiae *** on released genomic information,several programs were edited with PERL language to identify selenocysteine insertion sequence(SECIS)element,the coding potential of TGA codons,and cys-teine-containing homologs of selenoprotein *** results showed that 113.5 genes were termi-nated with TGA codons,918 of which contained SECIS *** search revealed that 58 genes contained Sec/Cys pairs and similar flanking regions around in-frame TGA ***,7 genes were found to fully meet requirements for selenoproteins,although they have not been anno-tated as selenoproteins in NCBI *** from their basic properties,the newly found se-lenoproteins in the genome of Anopheles gambiae are possibly related to in vivo oxidation tolerance and protein regulation in order to interfere with anopheles' vectorial capacity of *** study may also provide theoretical bases for the prevention of malaria from anopheles transmission.

关键词： selenoprotein selenocysteine Anopheles gambiae genome bioinformatics

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