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Synthesis of GoldMag particles with assembled structure and their applications in immunoassay

Science china Chemistry 2006年第6期49卷 534-540页

作者： CUI Yali1, ZHANG Lianying1, SU Jing1, ZHANG Caifeng3. LI Qi2, CUI Ting3. JIN Boquan2 & CHEN Chao1,3.1. Biochip Research and Development Center, Northwest university (National Engineering Research Center for Miniaturized Detection System), Xi’an 710069, china 2. Department of Immunology, The fourth {3. medical university, Xi’an 71003., china 3. Shaanxi Lifegen Co., Ltd., Xi’an 710069, china Biochip Research and Development Center Northwest University (National Engineering Research Center for Miniaturized Detection System) Xi’an China Shaanxi Lifegen Co. Ltd. Xi’an China Department of Immunology The Fourth Military Medical University Xi’an China

Micrometer-sized Fe3.4 particles and nano-sized gold particles were first synthesized by methods of self-aggregation of surface-chemically modified Fe3.4 nanoparticles and citrate reduction of the Au3. to Au0, respectively. Interaction between these two types of particles resulted in the assembly of nano-sized gold particles on the surface of the micrometer-sized Fe3.4 particles, forming an assembled structure with the Fe3.4 core particles around which are attached nano-sized gold parti- cles. The Fe3.4/Au structure is named GoldMag particles with assembled structure. The synthetic process, structure, and magnetic property of the GoldMag particles were analyzed. GoldMag particles with assembled structure have an irregular shape, rough surface with a diameter of 2―3.μm. These particles exhibit the superparamagnetic property with saturated magnetization of 41 A·m2/kg. In a single step, antibodies could be readily immobilized onto the surface of the particles with a high binding capacity. The GoldMag particles can be used as a novel carrier in immunoassays. The maximum quantity of human IgG immobilized onto GoldMag particles was 3.0 μg/mg. In order to validate the quality of the GoldMag particles as immunoassay carriers, an immunoassay system was used. The relative amount of immobilized human IgG was measured by HRP-labeled anti human IgG. The coefficient of variation within parallel samples of each group was below 6% and the coefficient of variation of means between five groups carried out separately was below 7%. Based on the sandwich method, the Hepatitis B surface antigen (HBsAg) and interleukin-8 (IL-8) were also analyzed by qualitative and quantitative detection, respectively. The result indicated that the GoldMag particles with assembled structure were an ideal carrier in immunoassay.

关键词： GoldMag particles with assembled structure, synthesis, carrier, immunoassay.

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Correlativity between neural complex and gonadal development in Styela plicata

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Chinese Science Bulletin 1997年第10期42卷 864-867页

作者： FANG Yongqiang, LIN Jiahan and HUANG Weiquan1. Third Institute of Oceanography, State Oceanic Administration, Xiamen 3.1005, china 2. Department of Biology, Xiamen U-niversity, Xiamen 3.1005, china 3. fourth military medical university, Xi’an 71003., china Third Institute of Oceanography State Oceanic Administration Xiamen China Department of Biology Xiamen University Xiamen China fourth military medical {3. Xi’an China

IT has been demonstrated that two reproductive hormones,gonadotropin-releasing hormone(GnRH)and gonadotropin(GTH),exist in the nervous system and Hatschek’s pit oflancelet,a species of Cephalochordata,and that these ... 详细信息

关键词： Styela plicata neural complex reproductive hormone.

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Growth inhibitory effect and apoptosis induced by extracellular ATP and adenosine on human gastric carcinoma cells: involvement of intracellular uptake of adenosine

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Acta Pharmacologica Sinica 2006年第8期27卷 1085-1092页

作者： Ming-xia WANG~2 Lei-ming REN~3.~2The fourth Affiliated Hospital,Hebei medical university,Shijiazhuang 050011,{3.,~3.chool of Pharmacy,Hebei medical university,Shijiazhuang 050017,{3. The fourth Affiliated Hospital Hebei Medical University Shijiazhuang China School of Pharmacy Hebei Medical University Shijiazhuang China

Aim:To study the growth inhibitory and apoptotic effects of adenosine triphos-phate(ATP)and adenosine(ADO)on human gastric carcinoma(HGC)-27 cells invitro and the mechanisms related to the actions of ATP and ***:MTTassay was used to determine the reduction of cell *** morphologicalchanges of HGC-27 cells induced by ATP or ADO were observed under fluores-cence light microscope by acridine orange/ethidium bromide double-stained *** internucleosomal fragmentation of genomic DNA was detected by agarosegel *** apoptotic rate and cell-cycle analysis after treatmentwith ATP or ADO was determined by flow ***:ATP,ADO and theintermediate metabolites,ADP and AMP,and the agonist of purinergic receptors,reduced cell viability of HGC-27 cells at doses of 0.3.and 1.0 mmol·*** distri-bution of cell cycle phase and proliferation index(PI)value of HGC-27 cells changedwhen exposed to ATP or ADO at the concentrations of 0.1,0.3.and 1 mmol/L for *** and ADO both altered the distribution of cell cycle phase via G0/G1-phasearrest and significantly decreased PI *** light microscope,the tumorcells exposed to 0.3.mmol·L-1ATP or ADO displayed morphological changes ofapoptosis;a ladder-like pattern of DNA fragmentation obtained from HGC-27 cellstreated with 0.1-1 mmol·L-1ATP or ADO appeared in agarose gel electrophoresis;ATP and ADO induced the apoptosis of HGC-27 ceils in a dose-dependent man-ner at concentrations between 0.03.1 *** maximum apoptotic rate ofHGC-27 cells exposed to ATP or ADO for 48 h was 13.53. or 15.9%,***-27 cell death induced by ATP or ADO was significantly inhibited by dipy-ridamole(10 mmol.L-1),an inhibitor of adenosine transporter,but was not affectedby aminophylline,a broad inhibitor of P1 receptors and pyridoxal-phosphate-6-azophenyl-2,4-disulphonic acid tetrasodium salt(3. μmol.L-1),a non-selectiveantagonist of P2 ***:Extracellular ATP and ADO reduced thecell

关键词： apoptosis adenosine triphosphate adenosine gastric carcinoma cells purinergic receptor

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Evaluation of 2 celecoxib derivatives:analgesic effect and selectivity to cyclooxygenase-2/1

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Acta Pharmacologica Sinica 2005年第12期26卷 1505-1511页

作者： Zhi-hong LU~(2,4) Xiao-yun XIONG~2 Bang-le ZHANG~2 Guo-cheng LIN~3.Yu-xiang SHI~4 Zhen-guo LIU~2 Jing-ru MENG~2 Yu-mei ZHOU~3.Qi-bing MEI~(3.6) 2 Department of Pharmacology,3.Department of Radioactive Isotopes,and 4 Department of Anesthesiology,Xijing Hospital,fourth military medical {3.,Xi'an 71003.,china 5 Department of Foreign Languages,fourth military medical {3.,Xi'an 71003.,china Department of Pharmacology Xijing Hospital Fourth Military Medical University Xi'an China Department of Foreign Languages Fourth Military Medical University Xi'an China Department of Anesthesiology Xijing Hospital Fourth Military Medical University Xi'an China Department of Radioactive Isotopes Xijing Hospital Fourth Military Medical University Xi'an China

Aim:To evaluate the analgesic effects of 2 celecoxib derivatives and their inhibi-tory effects on cyclooxygenase（COX）.Methods:Four antinociceptive assayswere used:the acetic acid-induced writhing test,hot plate test,hot tail-flick testand formalin *** doses were used in the analgesic assays and ED50valueswere *** the selectivity assay,macrophages were incubated with testcompounds at various concentrations and then stimulated with calcimycin orlipopolysaccharide（LPS）.The amounts of 6-keto-prostaglandin F1α(6-keto-PGF1α)and prostaglandin E2（PGE2）in the supernatant were examined by radioimmunoas-say（RIA）.The selectivity of the test compounds was expressed as the ***-1/***:Celecoxib and its 2 derivatives had a significant analge-sic *** ED50values of celecoxib,PC-406 and PC-407 were 94.2,67.9,and 63.3.g/kg,respectively,for the acetic acid-induced writhing test;104.7,89.1,and 3..0mg/kg,respectively,for the hot tail-flick response test;60.7,56.7,and 86.2 mg/kg,respectively,for the hot plate response test;67.1,55.8,and 68.8 mg/kg,respectively,for the formalin-induced *** is,the EDs0 of PC-406 was the lowest forthe formalin and hot plate tests,which focus on changes above the spinal cordlevel;however,the EDs0 of PC-407 was lowest for the tail-flick and writhing tests,which focus on changes at the spinal cord *** and PC-407 inhibitedCOX-1 with IC50values of 3..8 and 27.5 nmol/L,***-406 inhibitedCOX-1 with an IC50value of more than 1000 nmol/*** IC50values for the effectof celecoxib,PC-406 and PC-407 on COX-2 were 4.8,8.9,and 1.9 nmol/L *** ***-1]***-2ratios for celecoxib and PC-407 were 8.3.and 14.4,*** PC-406,the ratio was greater than ***:Derivatives ofcelecoxib via substitution with an isopropyl or naphthyl group at the 5 position inthe pyrazole ring still have analgesic effects and the ability to selectively *** wit

关键词： cyclooxygenase inhibitors celecoxib pain selectivity mice

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Probability parametric imaging with DCE-MRI data to identify prostate cancer in the peripheral zone

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Progress in Natural Science:Materials International 2006年第9期16卷 948-953页

作者： HUANG Xinrui, WANG Qiang, XU Yufeng, WANG Xiaoying and BAO Shanglian ( The Key Laboratory of medical Physics and Engineering, Peking university, Beijing 100871, china The medical School of Peking university, Beijing 100083. china 3. The First Hospital of Peking university, Beijing 10003., china) The Key Laboratory of medical Physics and Engineering Peking University Beijing 100871 China The medical School of Peking university Beijing 100083 China The First Hospital of Peking university Beijing 100034 China

To quantitatively analyze prostate dynamic contrast-enhanced magnetic resonance images (DCE-MRI), a retrospective study was carried out to construct a mathematical model to predict whether a lesion is cancerous or not. With known diagnosis of 52 re-gions-of-interest (10 malignant, 26 benign and 16 normal) in peripheral zone (PZ) of prostate from 18 patients undergoing needle biopsy and 8 normal volunteers, we combined feature parameters (k1, k2 and tp) from the linear-slope model with logistic regression to determine the statistical probability model. Prostate cancerous probability maps were derived using the model on each subject to examine the correlation between the model-based prediction and the known diagnosis. Compared the model prediction results of 52 regions-of-interest with the histopathologic results, the total agreement rate was 90.4 % ; the agreement rate was 60 % for the malignant and 97. 6 % for the nonmalignant. The diagnostic results only referencing the probability maps by two radiologists showed a high agreement in tissue characteristics and lesion location with the combining results from other diagnostic techniques such as PSA (prostate specific antigen) level and MRS (magnetic resonance spectroscopy) .

关键词： time-intensity curve prostate DCE-MRI logistic regression probability parametric image.

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A novel endorectal RF coil for high resolution MRI and spectroscopy of the prostate

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Progress in Natural Science:Materials International 2005年第5期15卷 472-476页

作者： ZHANG Hongjie 1,2, SONG Xiaoyu1, WANG Xiaoying3. XU Pingfang1 and BAO Shanglian1*(1. Beijing Key Laboratory of medical Physics & Engineering, Peking university, Beijing 100871, china 2. Navy General Hospital, Beijing 10003., china 3. Peking university First Hospital, Beijing 10003., china) Beijing Key Lab. of Med. Phys. and Eng. Peking Univ. Beijing 100871 China Navy General Hospital Beijing 100037 China Peking Univ. First Hospital Beijing 100034 China

The diagnosis of prostate diseases by magnetic resonance imaging (MRI) in vivo is sometimes difficult for the lower signal to noise ratio (SNR). To increase the SNR of the prostate image, we designed a RF coil that can be inserted into rectum and was named endorectal coil. The properties of RF coil were evaluated using a network analyzer. Moreover the images and spectroscopy of a special phantom were acquired and the results were compared to those of the commercial TORSO coil (G.E. medical Systems, USA). Our coil gave a significantly higher SNR at the region of interest (ROI). The achieved high local SNR and resulting high spatial resolution would add more anatomic and biochemical information to the diagnosis of prostate diseases.

关键词： MRI RF coil endorectal coil.

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Isoprenaline enhances local Ca2+ release in cardiac myocytes

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Acta Pharmacologica Sinica 2006年第7期27卷 927-932页

作者： Jian-xin SHEN~(2,3.42 Department of Physiology,Shantou university medical College,Shantou 515041,china 3.Department of Physiology,medical School of Xi'an Jiaotong university,Xi'an 710061,china Department of Physiology Shantou University Medical College Shantou China Department of Physiology Medical School of Xi'an Jiaotong University Xi'an China

Aim:Contraction of cardiac myocytes is controlled by the generation and ampli-fication of intracellular Ca2+*** key step of this process is the couplingbetween sarcolemma L-type Ca2+channels（LCCs）and ryanodine receptors（RyRs）in the sarcoplasmic reticulum（SR）.β-Adrenergic stimulation is an important regu-latory mechanism for this coupling *** the details underlied the globallevel,which require local Ca2+release study are still *** present study isto explore the effects of β-adrenergic stimulation on local Ca2+***:Using confocal microscopy combined with loose-seal patch-clamp approaches,effects of isoprenaline(1 μmol·L-1),a β-adrenergic agonist,on local SR Ca2+re-lease triggered by Ca2+influx through LCCs in intact rat cardiac myocytes ***:Isoprenaline increased the intensity of ensemble averagedlocal Ca2+transients,the peak of which displayed a typical bell-shaped voltage-dependence over the membrane voltages ranging from～-40mV to～+3.m ***-ther analysis showed that this enhancement could be explained by the increasedcoupling fidelity（which refers the increased probability of RyRs activation upondepolarization）,and the increased amplitude of evoked Ca2+sparks(due to moreCa2+releases through local RyRs).In addition,isoprenaline decreased the firstlatency,which displayed a typical"U"-shaped voltage-dependence,showing theavailable acceleration and synchronization of β-adrenergic stimulation on intrac-ellular calcium ***:Isoprenaline enhances local Ca2+release incardiac *** results underscore the importance of regulation of β-adrenergic stimulation on local intermolecular signals between LCCs and RyRs inheart cells.

关键词： local calcium release calcium signaling cardiac myocytes isoprenaline confocal microscopy

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Cytotoxic T lymphocyte response induced by an improved synthetic lipopeptide vaccine against cervical cancer

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Acta Pharmacologica Sinica 2007年第5期28卷 695-702页

作者： Dao-hua XU~(1,5),Chen-hui ZHOU~2,Yong-peng XIA~3.Zong-ying QIU~3.Yu-zhang WU~4,Zheng-cai JIA~4,Wei ZHOU~4~1 Department of Pharmacology and ~2 School of Nursing,Guangdong medical College,Zhanjiang 524023.china ~3.Department of PharmacyChongqing university of medical Science,Chongqing 400016,china,~4 Institute of Immunology PLA,Third military medical university,Chongqing 40003.,china Department of Pharmacology Guangdong Medical College Zhanjiang China School of Nursing Guangdong Medical College Zhanjiang China Department of Pharmacy Chongqing University of Medical Science Chongqing China Institute of Immunology PLA Third Military Medical University Chongqing China

Aim:To explore cytotoxic T lymphocyte （CTL） response induced by the lipopeptidevaccine against cervical ***:The immunological effect inducingCDS+ T cell-mediated cytotoxicity was investigated in human leukocyte antigen（HLA）-A2 transgenic mice and peripheral blood mononuclear cells （PBMC） ofhealthy HLA-A2.1+blood *** activity of specific CTL was measured byusing a standard 4 h51Cr release *** content of major histocompatibilitycomplex （MHC） I on T2 cells and the expression of immune molecules on dendriticcells （DC） were detected by flow cytometry,and the concentrations of interleukin（IL）-12 and interferon-γ were determined by ***:The lipopeptideinduced a strong epitope-specific CTL response both in vivo （transgenic mice）and in vitro （human PBMC）.This CTL induction was critically dependent on thepresence of the helper T lymphocyte epitope in transgenic mice,and the presenceof a lipid tail bypassed the need for an *** stability and persistence ofthe antigenic complex formed with the lipopeptide increased in comparison withthe CTL parental *** lipopeptide could induce the production of IL-12 inDC,but not the maturation of DC ***:The combination of CTLand the T helper epitope and lipid molecule can remarkably improve the immuno-genicity of the CTL peptide,the mechanism of which is associated with an in-crease in the stability and persistence of the antigenic complex formed with thelipopeptide and in the production of IL-12 in DC induced by the *** can be considered a more effective vaccine type for human being.

关键词： epitope cervical cancer lipopeptide vaccine

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Estrogen influences the differentiation, maturation and function of dendritic cells in rats with experimental autoimmune encephalomyelitis

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Acta Pharmacologica Sinica 2004年第4期25卷 110-115页

作者： Qing-hong ZHANG2, Yu-zhen HU, Jun CAO3. Yan-qing ZHONG,Yu-feng ZHAO, Qi-bing MEI4 Department of Physiology, Department ofOrthodontics, 3.4Department of Pharmacology, The fourth military medical {3., Xi’an 71003., china Department of Physiology The Fourth M ilitary Medical University Xi'an 710032 Department of Orthodontics the Fourth Military Medical University Xi'an 710032 Department of Pharmacology The Fourth Military Medical University Xi'an 710032

AIM: To examine if estrogen can affect the immune response at the dendritic cells (DCs) level in rats with experi- mental autoimmune encephalomyelitis (EAE). METHODS: Lewis rats were immunized with inoculum containing MBP68-86. DCs were derived from spleen monocytes of EAE rats with IL-4 and GM-CSF in presence of 17β- estradiol (E2). Nitric oxide (NO) was detected by Griess reagent. The surface markers and cytokines production of DCs were shown by flow cytometry. DCs were cocultured with MBP-specific T cells, [3.]-TdR incoportation was used to reveal the antigen presentability, the supernatant of the coculture were collected to examine the cytokines secretion by ELISA. RESULTS: E2 activated DCs by accelerating the maturation process characterized by upregulation of MHC II and costimulating molecule B7-1, B7-2, drastic high expression of CD40. IFN-γ–produc- ing DCs were also elevated without any alteration of IL-10. Estradiol-treated DCs (E2-DCs) secreted more NO in the culture supernatant. By contrast, E2-DCs showed decreased antigen presentation ability with reduced secretion of IFN-γ but no alteration of IL-10 in the coculture with T cells. CONCLUSION: Estrogen can affect the differentiation, maturation and function of DCs from EAE rats, which may be attributed to its protection against EAE and the remission of multiple sclerosis patients in pregnancy.

关键词： multiple sclerosis experimental autoimmune encephalomyelitis dendritic cells estrogens nitric oxide

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Immune Responses and Protective Efficacy Induced by 85B Antigen and Early Secreted Antigenic Target-6 kDa Antigen Fusion Protein Secreted by Recombinant Bacille Calmette-Guérin

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 290-296页

作者： Changhong SHI~1* Xiaowu WANG~1 Hai ZHANG~1 Zhikai XU~2 Yuan LI~2 Lintian YUAN~11 Laboratory Animal Researeh Center,fourth military medical {3.,Xi’an 71003.,china2 Department of Microbiology,fourth military medical {3.,Xi’an 71003.,china Laboratory Animal Research Center Fourth Military Medical UniversityXi'an 710033 China Department of Microbiology Fourth Military Medical UniversityXi'an 710033 China

In an attempt to improve immune responses and protective efficacy,we constructed tworecombinant bacille Calmette-Guérin(rBCG)strains expressing an 85B antigen(Ag85B)and early secretedantigenic target-6 kDa antigen(ESAT6)of M vcobacterium tuberculosis(MTB)fusion *** rBCGstrains have the same protein insertion but in a different order(Ag85B-ESAT6 and ESAT6-Ag85B).Thecultured supernatant of rBCG strains and the sera from the mice immunized with the fusion protein Ag85B-ESAT6 or ESAT6-Ag85B formed a band with a fraction size of 3. kDa,equalivalent to the sum of Ag85B *** weeks after BALB/c mice were immunized with BCG or rBCG,spleen lymphocytes showedsignificant proliferation in response to culture filtrate protein of *** with the BCG group,micevaccinated with rBCG elicited a high level increase of immunoglobulin G antibodies to culture filtrate proteinin the *** γ-interferon levels in the lymphocyte culture medium supernatants increased remarkably inthe rBCG1 group,significantly higher than that of the BCG immunized group(P3.Rv and a dramatic reduction in the numbers ofMTB colony forming units in the spleens and lungs was observed in the two rBCG immunization *** these rBCG strains were more immunogenic,their protective effect was comparable to the classicalBCG strain,and there were no significant differences between two rBCG groups(P>0.05).

关键词： Mycobacterium tuberculosis vaccine Ag85B ESAT6 BCG

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