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Distinguishing malignant from normal stomach tissues and its in vivo, in situ measurement in operating process using FTIR Fiber-Optic techniques

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Science china Chemistry 2005年第2期48卷 167-175页

作者： XU Yizhuang1, YANG Limin1, XU Zhi3. ZHAO Ying2, LING Xiaofeng3. LI Qingbo1, WANG Jiansheng4, ZHANG Nengwei3. ZHANG Yuanfu1 & WU Jinguang1 1. The State Key Laboratory of Rare Earth Materials and Applications, College of Chemistry and Molecular Engineering, peking university, beijing 100871, china 2. Institute of Chemistry, Chinese Academy of Sciences, beijing 100080, china 3. department of surgery, third hospital, peking university, beijing 100083, china 4. department of Oncology surgery, the First hospital of Xi’an Jiaotong {13., Xi’an 710061, china Correspondence should be addressed to Wu Jinguang (email: wujg@***) The State Key Laboratory of Rare Earth Materials and Applications College of Chemistry and Molecular Engineering Peking University Beijing China department of surgery Third Hospital Peking University Beijing China Institute of Chemistry Chinese Academy of Sciences Beijing China department of Oncology surgery the First Hospital of Xi’an Jiaotong University Xi’an China

Gastric tissue samples were studied using mid-IR fiber-optic attenuated total reflectance (ATR) spectroscopy. FTIR spectra of 90 tissue samples from 48 patients, including 3. normal and 58 malignant tissue samples, were chosen as examples. Malignancy was usually characterized by the absence of CH and C=O bands, a weak amide II band near 1545 cm-1, a shift of the amide I band to lower wave number, a decrease in the ～1450 cm-1 peak to less than the～1400 cm-1 peak. Subtraction spectra indicate that the amide I and amide II bands of normal and malignant tissues have larger differences in peak positions and relative intensities. The statistical analysis results confirm this conclusion. The results indicate that FTIR fiber optic techniques provide important information about cancerous tissue of the stomach, which can be used to differentiate the malignant tissue from the normal tissue. Based on the above results we successfully realize the detection of the tumor tissues of digestive tract in vivo and in situ. And the results of detection cancer near operating room and in vivo and in situ in the operating room are consistent with the conclusions for the samples stored in liquid N2, which is the basis for the clinical application.

关键词： FTIR, Fiber-Optics, stomach tissues, cancer, diagnosis.

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Expression of PDCD5 , a novel apoptosis related protein, in human osteoarthritic cartilage

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Acta Pharmacologica Sinica 2004年第5期25卷 143-148页

作者： Ai-xin CHENG, Si-quan LOU2, Hao-wei ZHOU, Ying WANG3. Da-long MA3.0} of Orthopaedic surgery, third hospital,{3., beijing 100083 3.enter for Human Disease Genomics,{3., beijing 100083, china department of Orthopaedic surgery Third Hospital Peking University 北京 100083 Center for Human Disease Genomics Peking University 北京 100083

AIM: To investigate the expression features of PDCD5 (programmed cell death 5 protein) in osteoarthritic andnormal human cartilage, and speculate on its potential functions in the pathogenesis of osteoarthritis (OA).METHODS: Articular cartilage specimens were obtained from 3. patients with OA and 16 healthy patients at thetime of arthroplasty. Expression of PDCD5 was detected by flow cytometry, immunofluorescence, RT-PCR andimmunohistochemical analysis. RESULTS: Enhanced expression and nuclear accumulation of PDCD5 in OAchondrocytes were found. PDCD5-positive chondrocytes were mainly distributed in the superficial and deep zonesof OA tissue sections, as opposed to, in the superficial and middle regions of normal healthy tissue ***: Since apoptotic chondrocyte death occurs more frequently in OA cartilage than in normal healthycartilage and PDCD5 is an apoptosis-related protein, the different expression patterns of PDCD5 in OA cartilagefrom that in normal healthy cartilage indicate that PDCD5 is involved in the pathogenesis of OA.

关键词： apoptosis cartilage osteoarthritis PDCD5

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Probability parametric imaging with DCE-MRI data to identify prostate cancer in the peripheral zone

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Progress in Natural Science:Materials International 2006年第9期16卷 948-953页

作者： HUANG Xinrui, WANG Qiang, XU Yufeng, WANG Xiaoying and BAO Shanglian ( The Key Laboratory of Medical Physics and Engineering, peking university, beijing 100871, china The Medical School of peking university, beijing 100083, china 3. The First hospital of peking university, beijing 10003., china) The Key Laboratory of Medical Physics and Engineering Peking University Beijing 100871 China The Medical School of peking university Beijing 100083 China The First hospital of peking university Beijing 100034 China

To quantitatively analyze prostate dynamic contrast-enhanced magnetic resonance images (DCE-MRI), a retrospective study was carried out to construct a mathematical model to predict whether a lesion is cancerous or not. With known diagnosis of 52 re-gions-of-interest (10 malignant, 26 benign and 16 normal) in peripheral zone (PZ) of prostate from 18 patients undergoing needle biopsy and 8 normal volunteers, we combined feature parameters (k1, k2 and tp) from the linear-slope model with logistic regression to determine the statistical probability model. Prostate cancerous probability maps were derived using the model on each subject to examine the correlation between the model-based prediction and the known diagnosis. Compared the model prediction results of 52 regions-of-interest with the histopathologic results, the total agreement rate was 90.4 % ; the agreement rate was 60 % for the malignant and 97. 6 % for the nonmalignant. The diagnostic results only referencing the probability maps by two radiologists showed a high agreement in tissue characteristics and lesion location with the combining results from other diagnostic techniques such as PSA (prostate specific antigen) level and MRS (magnetic resonance spectroscopy) .

关键词： time-intensity curve prostate DCE-MRI logistic regression probability parametric image.

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Up-regulation of Cartilage Oligomeric Matrix Protein Gene Expression by Insulin-like Growth Factor-Ⅰ Revealed by Real Time Reverse Transcription-Polymerase Chain Reaction

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 677-682页

作者： Hua TIAN~(1,2*) Ioannis STOGIANNIDIS~2 1 department of Orthopaedics,peking university{3.,beijing 100083,china 2 Coxa hospital for Joint Replacement,Biokatu 6 b,Tampere 3.101,Finland department of Orthopaedics Peking University Third HospitalBeijing 100083 China Coxa hospital for Joint Replacement Biokatu 6 bTampere 33101 Finland

Cartilage oligomeric matrix protein (COMP) strengthens cartilage by binding to type Ⅱ and typeⅨ collagen-forming bridges between collagen *** was hypothesized that perhaps one or more anabolicgrowth factors such as insulin-like growth factor-Ⅰ (IGF-Ⅰ),fibroblast growth factor-1 (FGF-1) or plateletderived growth factor-BB (PDGF-BB) increase COMP gene *** effects on primary humanchondrocytes and the chondrogenic cell line ATDC5 were studied using real time reverse transcript-poly-merase chain reaction (RT-PCR) for ***-Ⅰ,but not the FGF-1 or PDGF-BB,up-regulatedCOMP gene expression by approximate 5-fold in human adult chondrocytes in a dose-and ***-Ⅰ exerted similar effects on ATDC5 *** from these real time RT-PCR experimentswere confirmed by transfecting into ATDC5 cells a full-length mouse COMP promoter cloned upstream of aluciferase reporter *** stimulation with IGF-Ⅰ,the luciferase reporter activity increased by about *** conclusion,IGF-Ⅰ seems to be an important positive regulator of COMP,which may play animportant role in an attempted repair of either traumatized or degenerated cartilage.

关键词： cartilage oligomeric matrix protein (COMP) insulin-like growth factor-Ⅰ(IGF-Ⅰ) gene expression

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THE PHYSICAL ENTROPY OF SINGLE CONSERVATION LAWS

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Journal of Computational Mathematics 1998年第5期16卷 437-444页

作者： (department of Mathematics, peking university, beijing 100871,{3.) 北京大学数学系北京

By means of the comparisons with the formulas in statistical mechanics and thermodynamics, in this paper it is demonstrated that for the single conservation law partial derivative(t)u + partial derivative(x)f(u) = 0, if the flux function f(u) is convex (or concave), then, the physical entropy is S = -f(u); Furthermore, if we assume this result can be generalized to any f(u) with two order continuous derivative, from the thermodynamical principle that the local entropy production must be non-negative, one entropy inequality is derived, by which the O.A. Olejnik's famous E- condition can be explained successfully in physics.

关键词： conservation laws entropy entropy production

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Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells

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Acta Pharmacologica Sinica 2005年第8期26卷 963-968页

作者： Min YANG~2, Qing-jun MA~(2,4), Geng-ting DANG~2, Kang-tao MA~3. Ping CHEN~3. Chun-yan ZHOU~(3.4) ~2department of Orthopedics, peking university third hospital,{3., china ~3.0} of Biochemistry and Molecular Biology, School of Basic Medical Sciences, peking university Health Science Center,{3., china department of Orthopedics Peking University Third Hospital Beijing China department of Biochemistry and Molecular Biology School of Basic Medical Sciences Peking University Health Science Center Beijing China

Aim: To investigate the effects of bone morphogenetic protein-7 (BMP7)-expressing recombinant adeno-associated virus (AAV) vector on the differentiation of C2C12 cells. Methods: AAV-BMP7 was packaged by infecting the stable cell clone BHK-21 (integrated with recombinant AAV vector plasmid pSNAV-BMP7) with recombinant herpes simplex virus type 1, which expresses AAV-2 Rep and Cap and possesses AAV packaging functions. Following infection with AAV-BMP7 at multiplicities of infection of lx 10～5 vector genomes per cell and subse-quent culture, C2C12 cells were assessed qualitatively for BMP7 production, alka-line phosphatase activity, osteocalcin production and Cbfal and MyoD expression. Results: C2C12 cells transduced with AAV-BMP7 could produce BMP7 protein until d 28. Alkaline phosphatase in the cultured C2C12 cell lysate was elevated. Secreted osteocalcin in the culture medium was detectable at d 12 and Cbfal mRNA expression level was upregulated, coinciding with downregulation of MyoD in a temporal manner. Conclusion: The present in vitro study demonstrated that AAV-BMP7 could infect and efficiently convert C2C12 cells from myoblasts into osteoblast lineage cells.

关键词： C2C12 adeno-associated virus bone morphogenetic protein-7 gene therapy

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Beneficial metabolic effects of nateglinide versus acarbose in patients with newly-diagnosed type 2 diabetes

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Acta Pharmacologica Sinica 2007年第4期28卷 534-539页

作者： Hong-wei GAO Chao XIE Hai-ning WANG Yu-jing LIN Tian-pei HONG department of Endocrinology,peking university{3.,beijing 100083,china department of Endocrinology Peking University Third Hospital Beijing China

Aim:To investigate the acute and chronic effects of nateglinide versus acarboseon plasma asymmetric dimethylarginine (ADMA) levels and lipid profiles inpatients with newly-diagnosed type 2 ***:A crossover trial ofnateglinide and acarbose was conducted on 16 drug-nave patients with newly-diagnosed type 2 diabetes during a total period of 9 *** glucose,seruminsulin,free fatty acids (FFA),lipids and lipoproteins,and plasma ADMA ***:The efficiencies of a single dose of nateglinide (120 mg) andacarbose (50 mg) for lowering postprandial hyperglycemia were ***-pared to acarbose,nateglinide significantly increased postprandial insulin releaseafter a standard meal test in patients with type 2 *** acutelydecreased postprandial 120 min FFA concentrations and 240 min ADMA levelsmore significantly than *** fasting high-density lipoprotein choles-terol level increased and the low-density lipoprotein cholesterol level decreasedsignificantly,but the fasting levels of triglycerides,total cholesterol,and ADMAwere unchanged after 4 weeks of treatment with *** did notaffect fasting lipid profiles or the ADMA levels after 4 weeks of ***:These results suggest that the reduction of postprandial FFA andADMA concentrations induced by nateglinide may be associated with the partialrestoration of early-phase insulin secretion and may impart a cardiovascularadvantage in comparison with acarbose.

关键词： nateglinide acarbose free fatty acids asymmetric dimethylarginine

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AICAR inhibits proliferation and induced S-phase arrest,and promotes apoptosis in CaSki cells

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Acta Pharmacologica Sinica 2007年第12期28卷 1984-1990页

作者： Tong-ju GUAN~2,Feng-jin QIN~3.Jian-hai DU~4,Li Geng~3.You-yi ZHANG~(4,5),Min LI~(2,5)~2 Institute of Pathology,The School of Basic Medicine,Lanzhou university,Lanzhou 73.000,china ~3.department of Gynaecology andObstetrics,{3. university third hospital,beijing 100083,china ~4 Institute of Vascular Medicine,{3. university third hospital andKey Laboratory of Molecular Cardiovascular Sciences Ministry of Education,beijing 100083,china Institute of Pathology The School of Basic Medicine Lanzhou University Lanzhou China department of Gynaecology and Obstetrics Peking University Third Hospital Beijing China Institute of Vascular Medicine Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China

Aim:The aim of the present study was to determine the effect of 5-aminoimidazole-4-carboxamide-ribonucleoside （AICAR） on proliferation,cell cycle,and apoptosisin the human epithelial cervical cancer cell line CaSki ***:Cell countand 3.（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay were usedto determine cell proliferation and *** 3.258 staining was con-ducted to distinguish the apoptotic *** cycle and Annexin-V/propidiumiodide staining were analyzed by fluorescence-activated cell sorting （FACS）.AWestern blot assay was used to evaluate the expression of AKT （also known asprotein kinase B）,mammalian target of rapamycin （mTOR）,p53.and extracellularsignal-regulated kinase （ERK）.Results:AICAR （500 μmol/L） significantly inhibi-ted the proliferation of CaSki cells treated for 24,48,and 72 h as determined by *** cells at the G1and G2phases were dramatically decreased while cells atthe S phase were increased in response to AICAR treatment for 24,48,and 72 *** MTT assay showed less viable cells and Hoechst fluorescent staining showedmore apoptotic cells upon AICAR *** results of the Annexin-Vstaining demonstrated a time-dependent increase of apoptosis in cells treatedwith AICAR for 24,3.,and 48 ***,AICAR activated caspase-3.in atime-dependent *** was also found that AICAR inhibited the phosphory-lation of AKT and mTOR,which are important kinases regulating cell growth *** stimulation obviously increased the expression of the tumorsuppressor p53.and the phosphorylation of ***:AICAR inhibitedproliferation and induced S phase arrest and promoted apoptosis in CaSki cells,which might be mediated by the downregulation of the AKT/mTOR pathway andthe upregulation of the p53.ERK pathway.

关键词： AICAR proliferation apoptosis CaSki cells

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Petrogenesis of Songshugou dunite body in the Qinling orogenic belt, Central china: Constraints from geochemistry and melt inclusions

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Science china Earth Sciences 2005年第8期48卷 1146-1157页

作者： SU Li1,2, SONG Shuguang3.& ZHOU Dingwu1 1. Key Laboratory of Continental Dynamics of the Ministry of Education, department of Geology, Northwestern university, Xi’an 710069, china 2. The Geological Lab Center, china university of Geosciences (beijing), beijing 100083, china 3. Key Laboratory of Orogenic Belts of the Ministry of Education, department of Geology, peking university, beijing 100871, china Key Laboratory of Continental Dynamics of the Ministry of Education Department of Geology Northwestern University Xi’an China The Geological Lab Center China University of Geosciences (Beijing)Beijing China Key Laboratory of Orogenic Belts of the Ministry of Education Department of Geology Peking University Beijing China

The Songshugou dunite body, which occupies an area of about 20 km2, is the largest ultramafic massif in the eastern Qinling orogenic belt, Central china. The major compo-nent of this body is dunitic rocks including mylonitic dunite and coarse-grained dunite; they oc-cupy about 95 vol% of the total body. Petrography, mineral composition, major and trace ele-ments and primitive melt inclusions have been investigated in this paper; all revealed that this dunite body is the product of melt-rock interaction by porous percolation flow. In comparison with dunite sills or veins in harzburgite from the basal part of Oman ophiolite, this dunite body is characterized by lower contents of Al2O3. CaO and HREE but higher content of highly incom-patible elements and Zr and Hf. The LREE enriched patterns and primitive mantle normalized spidergrams of trace elements are also different from the Oman dunite. Combining with melt in-clusions observed in olivines, we conclude that this dunite body is the product of large- scale porous percolation flow of high-MgO melts within depleted mantle peridotites. The high-MgO magma, in essence, was most probably produced at the hot head of an upwelling mantle plume. The occurrence of the Songshugou dunite body is closely associated with the activity of mantle super-plume in Neo-Proterozoic era in the Yangtze Craton.

关键词： Songshugou dunite body, geochemistry, melt inclusion, mantle plume, Qinling orogenic belt.

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Dehydration melting of solid amphibolite at 2.0 GPa: Effects of time and temperature

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Science china Earth Sciences 2005年第8期48卷 1120-1133页

作者： ZHOU Wenge1, XIE Hongsen1, LIU Yonggang1, ZHENG Xiaogang1, ZHAO Zhidan2 & ZHOU Hui3.1. Laboratory of Material in the Earth’s Interior, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, china 2. department of Geology, china {3. of Geosciences, beijing 100083, china 3. department of Geology, peking university, beijing 100871, china Laboratory of Material in the Earth’s Interior Institute of Geochemistry Chinese Academy of Sciences Guiyang China department of Geology China University of Geosciences Beijing China department of Geology Peking University Beijing China

Two sets of dehydration-melting with a natural solid amphibolite, collected from North Himalayan structure zone, Tibet, have been carried out in multi-anvil apparatus at 2.0 GPa and 800―1000℃, for 12―200 h. One is keeping the pressure at 2.0 GPa and the annealing time of 12 h, changing the temperature (800―1000℃). The other is keeping the pressure at 2.0 GPa and temperature at 850℃, varying the annealing time (12―200 h). The products are inspected with microscope and electron probe. The results indicate that at 2.0 GPa, annealing time of 12 h, garnets, melts and clinopyroxenes occur in amphibolite gradually with increasing temperature and the chemical compositions of melt vary from tonalite to granodiorite, and then to tonalite. However, at 2.0 GPa and 850℃, with the annealing time increasing, the garnets, melts and cli-nopyroxenes also occur in amphibolite gradually and the chemical compositions of melt vary from tonalite to granodiorite. In both cases, melts interconnect with each other when the contents of melt are over the 5 vol.%. the viscosities of the melt produced in amphibolite at temperature higher than 850℃ are on a level with 104 Pa·s. The interconnected melt with such a viscosity may segregate from the source rock and form the magma over reasonable geological time. Therefore, it is believed that at the lower part of the overthickened crust, the tonlitic and grano-dioritic magma may be generated through the dehydration melting of amphibolite.

关键词： solid amphibolite, high pressure, high temperature, time, partial melting.

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