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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

Chinese Science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2,3.4, WANG Kemin1,2,3.4, XIAO Zhiqiang5,6, WANG Wei1,3.4, TAN Weihong1,2,3.4, SUN Yi5,6, TANG Hongxing1,3.4 & YANG Xiaohai1,3.41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan university, changsha 4.0082, china 2. Life Science and Biotechnology Institute,{3., Chang- sha 4.0082, china 3. chemistry and chemical engineer College,{3., changsha 4.0082, china 4. hunan engineering Research Center for Bio-Nanotechnology,{3., changsha 4.0082, china 5. Medical Experiment Research Center, Xiangya Hospital, Central South university, changsha 410008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, changsha 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life Science and Biotechnology Institute Hunan University Changsha China chemistry and chemical engineer College Hunan University Changsha China hunan engineering Research Center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research Center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53.RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53.with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Resolution of α-cyclohexyl-mandelic acid enantiomers by two-phase (O/W) recognition chiral extraction

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Science china chemistry 2007年第6期50卷 764-769页

作者： TANG KeWen1,2, ZHANG GuoLi2, HUANG KeLong2, LI Yuanjian3.& YI JianMin1,2 1 Department of chemistry and chemical engineering, {3. Institute of Science and Technology, Yueyang 414000, china 2 College of chemistry and chemical engineering, Central South university, changsha 410083. china 3.College of Pharmaceutical Science, Central South university, changsha 410078, china Department of chemistry and chemical engineering Hunan Institute of Science and TechnologyYueyang 414000China College of chemistry and chemical engineering Central South UniversityChangsha 410083China

This paper presents a new chiral separation technology:two-phase(O/W)recognition chiral *** behavior of alpha-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-isobutyl tartrate in 1,2-dichloroethane organic phase and beta-CD derivatives in aqueous phase,and the influence of the kind and concentration of extractant and pH on extraction performance was *** experimental results indicate that two-phase(O/W)recognition chiral extraction is of strong chiral separation ***-^-CD,HE-beta-CD and Me-beta-CD have higher recognition ability for S-CHMA than that for R-CHMA,among which HP-beta-CD has the strongest ability;whereas,D-isobutyl tartrate has reversed recognition ability for *** the extraction system containing HP-beta-CD and D-isobutyl tartrate,e.e.% of S-CHMA in aqueous phase reached 27.6% by one stage extraction,and the distribution ratio for R-CHMA(k_R)and for S-CHMA(k_S)and separation factor(alpha)are 2.44,0.89 and 2.49,***,pH and concentration of extractant have great effects on chiral separation ***-phase(O/W)recognition chiral extraction has great significance for preparative separation of racemic compounds.

关键词： two-phase(W/0)recognition chiral extraction alpha-cyclohexyl-mandelic acid enantiomers

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Formation and evolution mechanisms of large-clusters during rapid solidification process of liquid metal Al

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Science china(Physics,Mechanics & Astronomy) 2005年第1期48卷 101-112页

作者： LIU Rangsu, DONG Kejun, LIU Fengxiang, ZHENG Caixing, LIU Hairong & LI Jiyong Department of Applied Physics, hunan university, changsha 4.0082, china College of Materials Science and {3.ing, hunan university, changsha 4.0082, china College of chemistry and chemical {3.ing, hunan university, changsha 4.0082, china 1. Department of Applied Physics Human University 410082 Changsha China 2. College of Materials Science and engineering Hunan University 410082 Changsha China 3. College of chemistry and chemical engineering Hunan University 410082 Changsha China

A molecular dynamics simulation study has been performed for the formation and evolution characteristics of nano-clusters in a large-scale system consisting of 400000 atoms of liquid metal Al. The center-atom method combined with pair-bond analysis technique and cluster-type index method (CTIM) has been applied here to describe the structural configurations of various basic clusters. It is demonstrated that both the 1551 bond-type and the icosahedral cluster (12 0 12 0) constructed by 1551 bond-types are dominant among all the bond-types and cluster-types, respectively, in the system and play a critical role in the microstructure transitions of liquid metal Al. The nano-clusters (containing up to 150 atoms) are formed by the combination of some middle and small clusters with distinctly different sizes, through mutual competition by unceasing annex and evolution in a seesaw manner (in turn of obtaining and losing),which do not occur as the multi-shell structures accumulated with an atom as the center and the surrounding atoms are arranged according to a certain rule. This is the essential distinction of nano-cluster in liquid metal from those obtained by gaseous deposition, ionic spray methods, and so on. Though the nano-clusters differ from each other in shape and size, all of them possess protruding corners that could become the starting points of various dendrite structures in the solidification processes of liquid metals.

关键词： liquid metal, solidification process, large-cluster configuration, evolution mechanism, computer simulation.

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Drag reduction of flow boiling with polymer additives

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Journal of Central South university of Technology 2001年第2期8卷 143-146页

作者： QIU Yun ren 1,CHEN Wei ping 2,SI Qin 3.(1.College of chemistry and chemical engineering, Central South {3., changsha 410083. china 2.Editorial Office of Journal of Central South {3., changsha 410083. china 3.College of chemical E 1. College of chemistry and chemical engineering Central South University 410083 Changsha China 2. Editorial Office of Journal of Central South university 410083 Changsha China 3. College of chemical engineering Tianjin University 300072 Tianjin China

The drag reducing effect of polymer additive aqueous solution was investigated in flow boiling, and the polymer additives were two kinds of polyacrylamide (PAM) with relative molecular mass about 2.56×10 6 and 8.55×10 6. The frictional pressure drop was calculated according to the measured total pressure drop. The results show that the flow drag of flow boiling is reduced by adding a small amount of PAM to water when heat flux is in the range of 15.1 kW·m -2 to 47.0 kW·m -2 , when the mass fraction of PAM is higher than 2.0×10 -5 , the drag reducing effect is obvious. Drag reducing effect of PAM, whose relative molecular mass is 8.55×10 6, is slightly better than that of 2.56×10 6 at the same mass fraction, and the greater the flow rate of the additive solution, the better the effect of the drag reduction.

关键词： polymer additive flow boiling drag reduction pressure drop

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Piezoelectric transmission spectroelectrochemistry with a normal piezoelectric quartz crystal as an optically-transparent electrode

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Chinese Journal of chemistry 1994年第5期12卷 425-431页

作者： XIE, Qing-Ji PANG, Xiao-Yong NIE, Li-Hua YAO, Shou-Zhuo Department of chemistry and chemical engineering,{3., changsha, hunan 4.0082, china Department of chemistry and chemical engineering Hunan University Changsha Hunan 410082 China

Piezoelectric transmission spectroelectrochemistry (PTSEC), i.e., the combination of electrochemistry and spectroelectrochemistry (SEC) with electro{3. quartz crystal microbalance (EQCM) technique is reported by using a normal piezoelectric quartz crystal (PQC) as an optically-transparent electrode (OTE). A theoretical relationship between the PQC response and the spectroelectro{3. response is derived and used to estimate the apparent molar absorptivity of the absorbing species deposited on the OTE on the PQC surface. The complex of copper with aspartic acid is used to test this new PQC-SEC technique. Results show that the combination of three such diverse techniques provides a very useful methodology for studying electrode processes and electrode surface characteristics in situ.

关键词： Piezoelectronic transimission spectroelectrochemistry optically-transparent electrode electrochemical quartz crystal microbalance copper aspartic acid.

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Boron rings containing planar octa- and enneacoordinate cobalt, iron and nickel metal elements

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Science china chemistry 2008年第7期51卷 607-613页

作者： LUO Qiong School of chemistry and chemical engineering, Central South {3., changsha 410083. china School of chemistry and chemical engineering Central South UniversityChangsha 410083China

For a series of boron rings with planar hyper-coordinate 8th group transition metal atoms, singlet 1FeB8-2, multiplet kFeB9n (n = -1, k = 1; n = 0, k = 2), singlet 1CoB8n(n = -1, +1, +3., multiplet kCoB9n (n = +1, k = 2; n = 0, k = 1) and singlet 1NiB9+, the geometry structures have been optimized to be local minima on corresponding potential hyper-surfaces. The electron structures are discussed by orbital analysis and the aromaticity is predicted by nucleus-independent chemical shifts calculation at both the B3.YP/6-3.1+G* and BP86/6-3.1+G* levels of theory, respectively. The results suggest that all these structures with high symmetry planar geometries are stable and have aromatic properties with six π valence electrons.

关键词： planar hyper-coordinate transition metal DFT

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Fabrication,property characterization and toughening mechanism of HA-ZrO_2(CaO)/3.6L fibre composite biomaterials

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Science china(Technological Sciences) 2008年第7期51卷 957-967页

作者： ZOU JianPeng1, HE ZeQiang2, ZHOU ZhongCheng1, HUANG BaiYun1, CHEN QiYuan3.& RUAN JianMing1 1 State Key Laboratory for Powder Metallurgy, Central South university, changsha 410083. china 2 Institute of {3. and chemical engineering, Jishou university, Jishou 416000, china 3.Institute of {3. and chemical engineering, Central South university, changsha 410083. china State Key Laboratory for Powder Metallurgy Central South University Changsha 410083 China Institute of chemistry and chemical engineering Jishou University Jishou 416000 China Institute of chemistry and chemical engineering Central South University Changsha 410083 China

HA-ZrO2(CaO)/3.6L fibre composites were successfully fabricated with vacuum sintering method and their properties and toughening mechanism were studied. The results showed that HA-ZrO2(CaO)/3.6L fibre biocomposite having 20 vol% fibres had optimal comprehensive properties with bending strength, Young's modulus, fracture toughness and relative density equal to 140.1 MPa, 117.8 GPa, 5.81 MPa·m1/2 and 87.1%, respectively. The research also addressed that different volume ratios of the composites led to different metallographic microstructures, and that metallographic morphologies change regularly with volume ratios of the composites. 3.6L fibres were distributed randomly and evenly in the composites and the integration circumstance of the two phases was very well since there were no obvious flaws or pores in the composites. Two toughening mechanisms including ZrO2 phase transformation toughening mechanism and fibre pulling-out toughening mechanism existed in the compsites with the latter being the main toughening mechanism.

关键词： vacuum sintering fabrication property characterization toughening mechanism biomaterials

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Synthesis and purification of trialkyl compounds of gallium and indium

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Rare Metals 2002年第1期21卷 1-6页

作者： SHU Wangen, ZHOU Zhongcheng and LI Xiong chemistry and chemical engineering College, Central South {3., changsha 410083. china chemistry and chemistry engineering College Central South University Changsha 410083 chemistry and chemical engineering College Central South University Changsha 410083

The The research progress in trialkyl compounds of gallium andindium was discussed from two aspect, one was the chemical synthesisof the compounds and the other was the purification of them. Thereare three synthesis routes be- ing reported in the first aspect, *** route staring form pure metal, the route starting from the puremetal trihalides, and the electrochemical route. In the secondaspect, the purifying methods of decomposition-distillation and zonerefining were reviewed.

关键词： trialkyl compounds gallium nd indium synthesis purification

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Studies on the Synthesis, Properties and Preparation Methods of Zeolite ZK-4

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chemical Research in Chinese Universities 2000年第4期16卷 306-312页

作者： ZHAO Zhen-hua and YIN Du-lin (Department of chemistry, hunan Normal university, {3. 410081, P. R. china) hunan Normal Univ Dept Chem Changsha 410081 Peoples R China

A zeolite designated as ZK-4 was synthesized hydrothermally in the system (TMA)2ONa2O-Al2O3. SiO2- H2O at 80 - 100℃ with tetramethylammonium hydroxide as an organic templating agent. The pure zeolite ZK-4 could be prepared by using the mother liquor obtained after filtration as the source of templating agent. The effect of some synthetic conditions such as the amount of tetramethylammonium hydroxide used, n [ (TMA)2O + Na2O] /n (Al2O3. ratio, n (SiO2)/n(Al2O3. ratio and crystallization time, etc. on the crystalline phase of the product was investigated. When the following molar ratios of the reaction mixture, n (SiO2)/n (Al2O3. 4-10, n[(TMA)2O+Na2O]/ n (Al2O3.) > 7, n (Na2O)/n (Al2O3.=0. 5-2. 0, n (H2O)/ n(A12O3.=80-450, were used, pure zeolite ZK-4 could be obtained. The synthetic products were characterized by X-ray powder diffraction, atomic absorption spectrophotometry (AAS), adsorption measurement, thermogravimetric analysis(TGA), and 29Si magic angle spinning nuclear magnetic resonance technique. Zeolite ZK-4 has a higher thermal stability than sodium zeolite A due to higher n(Si)/n(Al) ratio of zeolite ZK-4.

关键词： Zeolite ZK-4 Molecular sieve Zeolite synthesis Property,Tetramethylammonium hydroxide template

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Characteristic atom arranging crystallogphy of alloy phases for Au-Cu system

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Science china(Technological Sciences) 2011年第6期54卷 1560-1567页

作者： XIE YouQing1,2,3.LIU XinBi1,2,3.PENG HongJian4,NIE YaoZhuang5,LI XiaoBo6 & LI YanFen1,2,3.1 School of Materials Science and engineering,Central South university,changsha 410083.{3. 2 Powder Metallurgy Research Institute,Central South university,changsha 410083.{3. 3.State Key Laboratory of Powder Metallurgy,Central South university,changsha 410083.{3. 4 School of chemistry and chemical engineering,Central South university,changsha 410083.{3. 5 School of Physical Science and Technique,Central South university,changsha,410083.{3. 6 College of Mechanical engineering,Xiangtan university,Xiangtan 411105,{3. 1. School of Materials Science and engineering Central South University Changsha 410083 China2. Powder Metallurgy Research Institute Central South University Changsha 410083 China3. State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China4. School of Chemistry and Chemical Engineering Central South University Changsha 410083 China5. School of Physical Science and Technique Central South University Changsha 410083 China6. College of Mechanical Engineering Xiangtan University Xiangtan 411105 China

In this paper,it is pointed out that the descriptions of alloy phase structures are dependent on structural unit *** the systematic science of alloys(SSA),the alloy phase structures are described by means of the symmetry element sequence combining with characteristic atom *** is named the characteristic atom arranging structure,which can display the characteristic atoms at the lattice sites and the micro-inhomogeneity,besides the *** characteristic atom has its own characters:neighboring configuration,potential energy,volume and electronic *** micro-inhomogeneity of alloy phases can be described by concentrations and short-range ordered parameters of characteristic *** differences between the electronic structures of alloy phases and electronic structures of characteristic atoms in the alloy phases are also discussed.

关键词： systematic science of alloys Au-Cu system alloy phase structure micro-inhomogeneity

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