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Top 10 Influential Events in carbon neutrality and climate change response for 2023

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Frontiers in Energy 2024年第1期18卷 1-3页

作者： research Institute of Carbon Neutrality of shanghai Jiao Tong University research Institute of Carbon Neutrality Shanghai Jiao Tong UniversityShanghai 200240China

In 2023. the global community intensified its efforts towards achieving carbon neutrality and addressing climate change, witnessing several impactful events. research Institute of Carbon Neutrality, shanghai Jiao Tong University compiled the Top 10 Events (in chronological order) in the field of global carbon neutrality and climate change response for 2023. to share with readers these significant moments that document the ongoing ambition and collective efforts of human society.

关键词： neutral collective intensified

TNF receptor-associated factor-2 binding site is involved in TNFR75-dependent enhancement of TNFR55-induced cell death

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Cell research 2001年第3期11卷 217-222页

作者： LU FANG, JIA FANG, CHANG QING CHEN shanghai research center of Biotechnology, shanghai Institutes for Biological sciences the chinese academy of sciences, shanghai 20023., china 1shanghai research center of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200233 China. 1shanghai research center of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200233 China. 1shanghai research center of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200233 China.

TNF recepter-55 is the main mediator of TNF-induced apoptosis. TNF receptor-75-dependent induction or enhancement of cytotoxicity has been explained by intracellular signaling, 'ligand passing' , or induction of endogenous TNF. To study the function of human TNF receptor-75 (hTR75) and the interaction between human TNF receptor-55 (hTR55) and hTR75 in hTNFa-induced cytotoxicity, HEp-2 cells were transfected with bicistronic expression vector of hTR75 and its deletion mutants genes. hTNFa-induced cytotoxicity was determined by crystal violet colorimetric method. The expression of hTR75 and its deletion mutants in HEp-2 cells was demonstrated by RT-PCR and indirect ELISA. We found that the overexpressed hTR75 could significantly increase the susceptibility of HEp-2 cells to hTNFa which especially required TRAF2 binding site. hTR75 could not only mediate partial hTNFa-induced cytotoxicity independently but also fulfill an accessory role in enhancing or synergizing hTR55-mediated cytotoxicity

关键词： hTNFa hTNF receptors TRAF2 cytotoxcity signal transduction.

Sequence analysis of mRNA polyadenylation signals of rice genes

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chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2, GAO Chenxi1,2,3.& HAN Bin1,3.41. National center for Gene research, shanghai Institutes for Biological {3., chinese academy of sciences, shanghai 20023., china 2. Graduate School of the chinese academy of sciences, Beijing 10003., china 3. Institute of Plant Physiology and Ecology, shanghai Institutes for Biological {3., chinese academy of sciences, shanghai 2.003., china National center for Gene research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China Graduate School of the chinese academy of sciences Beijing 100039 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3.-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12969 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953.genes. The al- ternative poly(A) sites were revealed in approxi- mately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3. fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3. fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

Association between Alcohol, Smoking and HLA-DQA1*0201 Genotype in Psoriasis

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 597-602页

作者： Guang-Yong ZHENG1,2#, Sheng-Cai WEI3., Tie-Liu SHI1, and Yi-Xue LI1* 1Bioinformatics center, shanghai Institutes for Biological sciences,{3., Graduate School of the{3., shanghai 2.003., china research center for Proteome Analysis, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological sciences, {3., Graduate School of the{3., shanghai 2.003., china 3.uangzhou Institute of Dermatology, Guangzhou 510095, china Bioinformatics center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Psoriasis is a chronic skin disease triggered by genetic, environment or other risk factors such as infection, drugs, stress, moisture, alcohol, and smoking. A major psoriasis susceptibility locus at 6p21.3. has been identified. Further studies found that HLA-DQA1*0201 allele was associated with psoriasis. However, there were few data exploring an association between the environmental factors and susceptibility genes. In this study, the samples of 189 patients with psoriasis and 3.3.healthy controls were collected with their consent and were carried on analysis through polymerase chain reaction sequence-specific primer (PCR- SSP) method. The proportion of male psoriasis patients engaging in the smoking and alcohol was much higher than that of the control group (P6.91, P<1.0×10–4).

关键词： psoriasis HLA-DQA1*0201 allele smoking alcohol association

Establishment of environmental specimen bank in shanghai

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Journal of Environmental sciences 2000年第4期12卷 398-401页

作者： ZHANG Yuan xun, WANG Yin song, QIAN Yin , TAN Ming guang, ZHI Ming, LI De yi, CHENG Feng, ZNANG Gui lin (shanghai Institute of Nuclear research, chinese academy of sciences, shanghai 201800, china) shanghai Institute of Nuclear research Chinese Academy of Sciences Shanghai 201800 China

The program of the shanghai Environmental Specimen Bank (SESB) has been established recently and its current status is briefly described. Typical specimen such as water and sediment of Suzhou creek, city particulate matter are collected and some results which have been obtained during the long term permanent operation of this project since 1998 are presented. Moreover, further aspects of environmental data evaluation and assessment are discussed.

关键词： specimen bank environment ecology

Calculation of the Surface Segregation of Cu-0.3.at. pct Au Alloy

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Journal of Materials Science & Technology 2000年第6期16卷 639-640页

作者： Liping ZHENG and Risheng LI (International center for Material Physics, chinese academy of sciences, Shenyang 110015, china) Yuanxun ZHANG and Shi QIU ({3. Institute of Nuclear research, chinese academy of sciences, {3. 201800, china) Bingyao JI Int. Cent. for Material Phys. Chinese Acad. of Sci. Shenyang 110015 China

Monte Carlo simulation technique with the energetics described by the embedded atom method has been used to calculate the surface segregation of Cu-0.3.at. pct Au alloy at T=800 K. It is found that the component Au in... 详细信息

关键词： Cu Calculation of the Surface Segregation of Cu-0.3.at pct Au Alloy

Functional analysis of schistosomes EF-hand domain-containing tegument proteins

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chinese Science Bulletin 2007年第15期52卷 2100-2107页

作者： YU FuDong1,2,3.KANG Bin1,3.LI YuanYuan3.4 & LI YiXue1,3.1 Bioinformatics center,Key Laboratory of Systems Biology,shanghai Institutes for Biological sciences,{3. academy of Sci-ences,shanghai 2.003.,china 2 Graduate University of chinese academy of sciences,shanghai 2.003.,china 3.shanghai center for Bioinformation Technology,shanghai 20023.,china 4 Key Laboratory of Systems Biology,shanghai Institutes for Biological sciences,{3. academy of sciences,shanghai 2.003.,china Bioinformatics center Key Laboratory of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Schistosomes cause schistosomiasis disease which severely threatens human health. Little is known about the functions of EF-hand domain containing schistosomes tegument proteins other than as an-tigens. More possible functions of these tegument proteins were investigated with in silico analyses including protein-protein functional interaction,site-specific variation and glycosylation modification. The analysis results suggested that schistosomes could actively modulate host immune responses for its own favor through functional interactions with host proteins with immunomodulatory function,and passively regulate host immune responses through sequence variation under positive selection and glycosylating the recognition sites of host immune attack. In addition,the analysis of the C-terminal domain of these tegument proteins indicated that they could assist schistosomes in escaping host immune attacks through inhibiting chemotaxis and non-complement fixing antibody (IgG4) responses. In summary,our results suggested that these tegument antigen proteins could assist schistosomes in escaping and modulating host immune responses for self-protection during the process of host-para-site interaction.

关键词：寄生虫血吸虫皮蛋白质健康

Conus Peptides-A Rich Pharmaceutical Treasure

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 713-723页

作者： Cheng-Zhong WANG and Cheng-Wu CHIInstitute of Biochemistry and Cell Biology, shanghai Institute of Biological sciences, the chinese academy of sciences,{3., china Institute of Neuroscience, the chinese academy of sciences,{3., china Institute of Protein research, Tongji University, shanghai 200092, china Institute of Biochemistry and Cell Biology Shanghai Institute of Biological Sciences the Chinese Academy of SciencesShanghai 200031 China Institute of Neuroscience the Chinese Academy of SciencesShanghai 200031 China Institute of Protein research Tongji UniversityShanghai 200092 China

Marine predatory cone snails (genus Conus) with over 500 species represent what is arguably the largest single genus of marine animals alive today. All Conus are venomous and utilize a complex mixture of Conus peptide... 详细信息

关键词： Conus peptide conotoxin neuropathic disorder pharmaceutical potency

Recombinant scFv Antibodies against E Protein and N Protein of Severe Acute Respiratory Syndrome Virus

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 541-547页

作者： Hui LIU , Yan-Li DING, Wei HAN, Mei-Yun LIU, Rui-Yang TIAN, Sheng-Li YANG, and Yi GONGshanghai research center of Biotechnology, shanghai Institutes of Biological sciences, chinese academy of sciences,Graduate School of the chinese academy of sciences, shanghai 20023., china shanghai research center of Biotechnology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200233 China. huiliu@***

Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, Kd) of SARS-CoV E protein was 5.7×10–8 M for B10 and 8.9×10–8 M for C20. The affinity of A17 for N protein was 2.1×10–6 M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot.

关键词： SARS-CoV antibody scFv phage display affinity chromatography