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Top 10 Influential Events in carbon neutrality and climate change response for 2.2.

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Frontiers in Energy 2024年第1期18卷 1-3页

作者： research Institute of Carbon Neutrality of shanghai Jiao Tong University research Institute of Carbon Neutrality Shanghai Jiao Tong UniversityShanghai 200240China

In 2.2.0} the global community intensified its efforts towards achieving carbon neutrality and addressing climate change, witnessing several impactful events. research Institute of Carbon Neutrality, {2. Jiao Tong University compiled the Top 10 Events (in chronological order) in the field of global carbon neutrality and climate change response for 2.2.0} to share with readers these significant moments that document the ongoing ambition and collective efforts of human society.

关键词： neutral collective intensified

Possible Role of Histone H1 in the Regulation of Furin-dependent Proprotein Processing

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 173-180页

作者： Jinbo HAN~1 Jianxin GU~2.Chengwu CHI~(1,3.) 1 Institute of Biochemistry and Cell Biology,chinese {2. of Sciences,Graduate School of the chinese {2. of Sciences,shanghai 2.003.,china 2.Gene research center,Medical center of Fudan University,shanghai 2.003.,china 3.Institute of Protein research,Tongji University,shanghai 2.0092.china Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China.

Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitorsof furin,a mammalian proprotein ***,its role in the regulation of furin-dependent proproteinprocessing remains *** we report that histone H1 efficiently blocks furin-dependent pro-yonWillebrand factor（pro-vWF）processing in a dose-dependent *** and immunof-luorescence studies confirmed that histone H1 could interact with furin,and the interaction mainly took placeon the cell *** noted that histone H1 was released from cells undergoing necrosis and apoptosisinduced by *** findings suggested that histone H1 might be involved in extracellular and/or intracellu-lar furin regulation.

关键词： furin histone H1 pro-vWF proprotein processing

Sequence analysis of mRNA polyadenylation signals of rice genes

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chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2. GAO Chenxi1,2.3.& HAN Bin1,3.41. National {2. for Gene research, shanghai Institutes for Biological Sciences, {3. academy of Sciences, shanghai 2.02.0}, china 2. Graduate School of the {3. academy of Sciences, Beijing 10003., china 3. Institute of Plant Physiology and Ecology, shanghai Institutes for Biological Sciences, {3. academy of Sciences, shanghai 2.003., china National center for Gene research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of Sciences Beijing China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3.-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12.69 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953.genes. The al- ternative poly(A) sites were revealed in approxi- mately 2.% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3. fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3. fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

Expression of Monomeric Insulin Precursor in Pichia pastoris and Its Conversion into Monomeric B2. Lys Destripeptide Insulin by Tryptic Hydrolysis

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3. Jian FEI~2.Da-Fu CUI~1 You-Shang ZHANG~1 ~1Institute of Biochemistry and Cell Biology,and ~2.odel Organism research {2.,shanghai Institutes for Biologicol Sciences, {3. academy of Sciences,shanghai 2.003.,china ~3.raduate School of the {3. academy of Sciences,shanghai 2.003.,china Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Graduate School of the chinese academy of Sciences {2. 2.003. China Model Organism research center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Monomeric B2. Lys destripeptide insulin (B2. Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B2. Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B2. Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B2. Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 2.0 mg per liter of culture,and the overall yield ofpurified B2. Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B2. Lys DTrI asdetermined by the mouse convulsion assay was 2. U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B2. Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

CO observations of massive star forming regions at different evolutionary phases

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Science china(Physics,Mechanics & astronomy) 2006年第5期49卷 626-640页

作者： CHEN Xi1,2. XU Ye1,3. SHEN Zhiqiang1,3.& LI Jingjing1,2.1. {2. astronomical Observatory, chinese {3. of Sciences, shanghai 2.0030, china 2. Graduate School of the chinese {3. of Sciences, Beijing 10003., china 3. Joint Institute for Galaxy and Cosmology, {2. astronomical Observatory and the University of Science and Technology of china, shanghai 2.0030, china shanghai astronomical Observatory Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of Sciences Beijing China Joint Institute for Galaxy and Cosmology Shanghai Astronomical Observatory and the University of Science and Technology of China Shanghai China

The 12.O (J=1-0), 13.O (J=1-0) and C18O (J=1-0) emissions in 9 massive star forming regions, which are believed to be at different stages of massive star formation, were mapped with the 13.7 m millimeter wave telescope at Qinghai Station of Purple Mountain Observatory. Of the observed 9 sources, 13.O cores were detected in seven of them, and C18O cores in five of them. And only two sources associated with C18O cores and H2. masers showed the extended structures and strong outflows. This is the first detection of outflow associated with IRAS 2.566+582. in the observing field of S152.S153. The physical parameters of cores and outflows for these sources, derived from Local Thermal Equilibrium (LTE) analysis, are presented. These observing results suggest that the C18O cores will only appear when the gas density is high enough and the probability to have an outflow is very high when the clumps show the C18O and H2. maser simulta- neously.

关键词： star formation cloud core outflow H2. maser

Integrated precise orbit determination of Shenzhou IV unmanned spacecraft

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Science china(Technological Sciences) 2004年第5期47卷 518-525页

作者： L Congmin1,2. GU Yidong3. LIN Baojun3.& GUO Jiong3.1. center for Space Science and Applied research, chinese {3. of Sciences, Beijing 100080, china 2. Graduate School of the chinese {3. of Sciences, Beijing 10003., china 3. General Establishment of Space Science and Application, chinese {3. of Sciences, Beijing 100080, china Correspondence should be addressed to L Congmin center for Space Science and Applied research Chinese Academy of Sciences Beijing China Graduate School of the chinese academy of Sciences Beijing China General Establishment of Space Science and Application Chinese Academy of Sciences Beijing China

Considering the characteristic orbit of Shenzhou IV spacecraft and the advantages and disadvantages of several types of domestic orbit measurements, a scheme of IPOD is determined, which uses GPS, SLR and USB observation data jointly plus precise ionosphere corrections to GPS observation data. IPOD system and mathematic models of OD are introduced. The results of different joint OD are analyzed using true observation data, and show that radial accuracy is better than 2.m.

关键词： Shenzhou IV unmanned spacecraft integrated precise orbit determination GPS SLR USB.

Comparative Analysis of Two-component Signal Transduction System in Two Streptomycete Genomes

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 317-325页

作者： WU WEI~(1,3.), Weihua WANG~(2.), Zhiwei CAO~(3.), Hong YU~3. Xiaojing WANG~1, Jing ZHAO~3. Hao TAN~3. Hao XU~3. Weihong JIANG~(2.), and Yixue LI~(1,3.) 1 Bioinformation center, Key Lab of Systems Biology, shanghai Institutes for Biological Sciences, chinese {3. of Sciences, Graduate School of the chinese {3. of Sciences, shanghai 2.003., china 2.Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, shanghai Institutes for Biological Sciences, chinese {3. of Sciences, Graduate School of the chinese {3. of Sciences, shanghai 2.003., china 3.shanghai center for Bioinformation Technology, shanghai 2.02.0}, china Bioinformation center Key Lab of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China shanghai center for Bioinformation Technologyshanghai 2.02.3} China Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200032 China

Species of the genus Streptomyces are major bacteria responsible for producing most natural antibiotics. Streptomyces coelicolor A3.2. and Streptomyces avermitilis were sequenced in 2.02.and 2.03. respectively. Two-component signal transduction systems (TCSs), consisting of a histidine sensor kinase (SK) and a cognate response regulator (RR), form the most common mechanism of transmembrane signal transduction in prokaryotes. TCSs in S. coelicolor A3.2. have been analyzed in detail. Here, we identify and classify the SK and RR of S. avermitilis and compare the TCSs with those of S. coelicolor A3.2. by computational approaches. Phylogenetic analysis of the cognate SK-RR pairs of the two species indicated that the cognate SK-RR pairs fall into four classes according to the distribution of their orthologs in other organisms. In addition to the cognate SK-RR pairs, some potential partners of non-cognate SK-RR were found, including those of unpaired SK and orphan RR and the cross-talk between different components in either strain. Our study provides new clues for further exploration of the molecular regulation mechanism of streptomycetes with industrial importance.

关键词： Streptomyces two-component system cross-talk phylogenetic analysis

PIXE and INAA studies on ancient potteries from Guangfulin relics in shanghai

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Progress in Natural Science:Materials International 2007年第11期17卷 1348-1356页

作者： Lin Jiawei1, Cheng Huansheng1, Zhang Zhengquan1, Zhang Bin1, Feng Songlin2. Song Jian3. Gao Menghe4 and Zhu Dan1(1. Applied Ion Beam Physics Laboratory, Key Laboratory of the Ministry of Education, Institute of Modern Physics, Fudan University, shanghai 2.043., china 2. Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Beijing 10003., china 3. shanghai Museum, shanghai 2.0003. china 4. Department of Cultural Heritage and Museology, Fudan University, shanghai 2.043., china) Applied Ion Beam Physics Laboratory Institute of Modern Physics Fudan University Shanghai 200433 China Key Laboratory of Nuclear Analytical Techniques Institute of High Energy Physics Beijing 100039 China shanghai Museum Shanghai 200003 China Department of Cultural Heritage and Museology Fudan University Shanghai 200433 China

This paper reports the study of ancient potteries unearthed from the Guangfulin site in shanghai. From the external features of these potteries, archeologists can classify them into two different cultural types: Liangzhu culture (local culture) and a new culture which might be derived elsewhere, but they can not recognize whether they were produced in the same place. Proton induced X-ray emission (PIXE) and instrumental neutron activation analysis (INAA) were used to measure the chemical compositions of the samples and the results were analyzed using multivariate statistics. We found that the two methods can complement each other and their database can be mutually referenced in the study of ancient potteries. Experimental results show that the compositions of the potteries from the two cultural types are different, which means that the materials for ancient potteries of new culture originate from elsewhere. This supports the archaeologists’ speculation that a group of ancient people migrated to shanghai from some other places 4000 years ago.

关键词： PIXE INAA factor analysis Guangfulin ancient pottery

Development of a quantitative,cell-based,high-content screening assay for epidermal growth factor receptor modulators

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Acta Pharmacologica Sinica 2007年第10期28卷 1698-1704页

作者： Jue WANG~2.Xin XIE~(2.3.~2.The National center for Drug Screening,{2. Institute of Materia Medica,chinese {3. of Sciences Graduate School of chinese {3.of Sciences {2. 2.12.3.china The National center for Drug Screening Shanghai Institute of Materia Medica Chinese Academy of Sciences Graduate School of chinese academy of Sciences Shanghai China

Aim:To develop a robust,cell-based,high-content screening （HCS） assay basedon receptor internalization for the identification of novel modulators of the epi-dermal growth factor receptor （EGFR）.Methods:Agonist-induced receptorinternalization is part of the signaling cascade of ***-taggedepidermal growth factor （EGF） was used to visualize the internalized receptor-ligand *** fluorescent intracellular spots were detected and mea-sured with an ArrayScan HCS *** that can competitively bind toEGFR or interfere with EGFR internalization process would result in a reducednumber and intensity of intracellular fluorescent *** assay was validated,optimized,and applied to a large-scale screening of a library containing 48 000synthetic ***:The competition between fluorescent EGF andunlabeled EGF reveals the IC50of unlabeled EGF is approximately 0.2.nmol/L,which is comparable with other published *** compounds with arelatively high degree of interference with EGFR internalization were *** of the compounds was proven to be agonist of the EGFR since it inducedphosphorylation of the receptor and extracellular signal-regulated protein ki-nase （ERK）.Conclusion:This automated,objective,and easy-to-use assay pro-vided abundant information,quantitative results,and demonstrated the potentialuse of HCS methods in searching membrane receptor modulators.

关键词： high-content screening epidermal growth factor receptor receptor internalization competitive binding