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Altered expression of cytochrome P450 and possible correlation with preneoplastic changes in early stage of rat hepatocarcinogenesis

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Acta Pharmacologica Sinica 2005年第6期26卷 737-744页

作者： Lin-lin LIU~(2,3., Li-kun GONG~(2,3., Xin-ming QI~(2,3.,Yan CAI~(2,3., Hui WANG~2, Xiong-fei WU~(2,3.,Ying XIAO~(2,3., Jin REN~(2,4) ~2 State Key Laboratory of Drug research, shanghai Institute of Materia Medica, shanghai Institutes for Biological {3., chinese academy of sciences, shanghai 201203. china ~3.Graduate School of the chinese academy of sciences, shanghai 201203. china State Key Laboratory of Drug research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of sciences Shanghai China

Aim: Correlation of cytochrome P450 (CYPs) with preneoplastic changes in the early stage of hepatocarcinogenesis is still unclear. To detect the expression of carcinogen-metabolizing related microsomal P450 enzymes, namely the CYP1 Al, CYP1A2, CYP2B1/2, CYP2E1, and CYP3., we performed the medium-term bioas-say of Ito's model in Sprague-Dawley rats. Methods: The amount and activity of CYP were assessed by biochemical and immunohistochemical methods in week 8. The correlation between CYP expression and microsomal oxidative stress was investigated by comparing the generation of microsomal lipid peroxidation in the presence or absence of specific CYP inhibitor. Results: In the DEN-2-AAF and 2-AAF alone groups, the expression of CYP1 Al and CYP2E1 were up-regulated and the expression of CYP2B1/2 and CYP1A2 were quite the contrary. Strong staining of CYP2E1 and CYP2B1/2 was found around the centrolobular vein and weak staining in the altered hepatic foci revealed by immunohistochemical procedure. There was no significant change in the activity of CYP3. among the 4 groups. Altered hepatic tissue bore more microsomal NADPH (nicotinamide adenine dimucleotide phosphate,reduced form)-dependent lipid peroxidation than normal tissue. And the difference among the 4 groups disappeared when CYP2E1 was inhibited. More microsomal lipid peroxidation was generated when incubated with CYP1A inhibitor a-naphthoflavone. Conclusion: CYP altered their expression levels and these alterations can play important roles in the alteration of cell redox status of preneoplastic tissue in the early stage of hepatocarcinogenesis.

关键词： cytochrome P450 preneoplastic changes hepatocarcinogensis

Axon guidance and neuronal migration research in china

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Science china(Life sciences) 2010年第3期53卷 304-314页

作者： YUAN XiaoBing Institute of Neuroscience,shanghai Institutes for Biological sciences,chinese {3. of sciences,shanghai 20003.,china Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Proper migration of neuronal somas and axonal growth cones to designated locations in the developing brain is essential for the assembly of functional neuronal *** progress in research of axon guidance and neuronal migration has been made in the last twenty *** researchers began their exploration in this field ten years ago and have made significant contributions in clarifying the signal transduction of axon guidance and neuronal *** unique experimental approaches,including the migration assay of single isolated neurons in response to locally delivered guidance cues,have been developed by chinese neuroscientists to investigate the molecular machinery underlying these guidance events.

关键词： axon guidance neuronal migration development neuronal circuits

In Vitro Reassembly of Tobacco Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Fully Denatured Subunits

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 737-745页

作者： Zhen-Hua YONG Gen-Yun CHEN* Jiao-Nai SHI Da-Quan XU Institute of Plant Physiology and Ecology,shanghai Institutes for Biological sciences,chinese {3. of sciences,and Graduate School of the chinese academy of sciences,shanghai 20003.,china Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Graduate School of the Chinese Academy of SciencesShanghai 200032 China

It has been generally proved impossible to reassemble ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubisco） from fully denatured subunits in vitro in higher plant,because large subunit of fullydenatured Rubisco is liable to precipitate when the denaturant is removed by common methods of directdilution and one-step *** our experiment,the problem of precipitation was resolved by an improvedgradual dialysis method,which gradually decreased the concentration of ***,fully denaturedRubisco subunits still could not be reassembled into holoenzyme using gradual dialysis unless chaperonin 60was *** restored activity of reassembled Rubisco was approximately 8% of natural *** of reassembled Rubisco increased greatly when heat shock protein 70 was present in the *** and Mg2+ were unnecessary for in vitro reassembly of Rubisco,and Mg2+ inhibited the *** reassembly was weakened when ATP,Mg2+ and K+ existed together in the reassembly process.

关键词： Rubisco reassembly gradual dialysis chaperonin 60 Hsp70

Cloning and characterization of vernalization-related gene (ver203.) cDNA 3.end

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chinese Science Bulletin 1999年第14期44卷 1289-1294页

作者： YONG Weidong, CHONG Kang , LIANG Tiebing, XU Zhihong , TAN Kehui and ZHU Zhiqing research center for Molecular and Developmental Biology, Institute of Botany, chinese academy of sciences , Beijing 100093.{3. shanghai Institute of Plant Physiology , chinese academy of sciences , shanghai 20003.,{3. Corresponding authors research center for Molecular and Developmental Biology Institute of Botany Chinese Academy of Sciences Beijing China shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China

Based on the cDNA fragment sequence of vernalization-related gene verc203.cloned by differential screening in our lab, the 5′primer has been designed. The cDNA 3.end of ver203.gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the gene has homology with Hordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate.

关键词： wheat vernalization-related gene cDNA 3. end RACE.

The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider Ornithoctonus huwena

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 656-660页

作者： Peng QIAO, Xiao-Pan ZUO, Zhi-Fang CHAI, and Yong-Hua JI* Institute of Physiology, shanghai Institutes for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 20003., china Institute of Physiology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

In present study the full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus huwena, named as SHT-I, has been cloned using the strategy of rapid amplification of cDNA ends, and then the whole genomic sequence of SHT-I (Selenocosmia huwena toxin-I) was determined using sequence-specific primers synthesized based on the acquired 3. and 5’ ends of SHT-I cDNA sequence. It is unexpectedly found that intron was lacking in the genomic sequence of SHT-I. The result might evoke an interesting question whether the gene code of inhibitor cystine-knot peptides from spider venom is distinct from that of those known toxic peptides of scorpion and cone snail.

关键词： Ornithoctonus huwena spider cDNA genomic DNA peptide toxin ICK motif

Hyaluronan Promotes Tumor Lymphangiogenesis and Intralymphantic Tumor Growth in Xenografts

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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 601-606页

作者： Li-Xia GUO Ke ZOU Ji-Hang JW Hong XIE Laboratory of Biotherapy,Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological sciences,chinese {3. of sciences,Graduate School of the chinese academy of sciences,shanghai 20003.,china Laboratory of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

Hyaluronan (HA),a high molecular weight glycosaminoglycan in the extracellular matrix,hasbeen implicated in the promotion of malignant phenotypes,including tumor ***,little isknown about the effect of HA on tumor-associated *** this study,mouse hepatocellularcarcinoma Hca-F cells combined with or without HA were injected subcutaneously into C3./Hej mice,thenangiogenesis and lymphangiogenesis of implanted tumors were examined by immunostaining for platelet-endothelial cell adhesion molecule-1 and lymphatic vascular endothelial hyaluronan receptor-1 ***,we found HA promotes tumor lymphangiogenesis and the occurrence of intratumoral lymphaticvessels,but has little effect on tumor ***,HA also promotes intralymphatic tumorgrowth,although it is not sufficient to potentiate lymphatic *** results suggest that HA,which is elevated in most malignant tumor stroma,may also play a role in tumor progression by promotinglymphangiogenesis.

关键词： hyaluronan tumor lymphangiogenesis intralymphatic tumor growth

Inhibition of Tumor Growth in Mice by Endostatin Derived from Abdominal Transplanted Encapsulated Cells

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 278-284页

作者： Huaining TENG~(1.3. Ying ZHANG~2 Wei WANG~2 Xiaojun MA~2* Jian FEI~1* 1 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Model Organism research center,shanghai Institutes for Biological {3.,chinese academy of {3.,shanghai 20003.,china 2 Laboratory of Biomedical Material Engineering,Dalian Institute of Chemical Physics,chinese academy of {3.,Dolian 116023.china 3.Department of Urology,Changzheng Hospital,Second Military Medical University,shanghai 200003.china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Department of Urology Changzheng Hospital Second Military Medical UniversityShanghai 200003 China Laboratory of Biomedical Material Engineering Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023 China

Endostatin,a C-terminal fragment of collagen 18a,inhibits the growth of established tumorsand metastases in vivo by inhibiting ***,the purification procedures required for large-scale production and the attendant cost of these processes,together with the low effectiveness in clinicaltests,suggest that alternative delivery methods might be required for efficient therapeutic use of *** the present study,we transfected chinese hamster ovary(CHO)cells with a human endostatin geneexpression vector and encapsulated the CHO cells in alginate-poly-L-lysine *** release ofbiologically active endostatin was confirmed using the chicken chorioallantoic membrane *** encap-sulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16tumor model when injected into the abdominal cavity of *** results widen the clinical applicationof the microencapsulated cell endostatin delivery system in cancer treatment.

关键词： microencapsulation endostatin CHO tumor gene therapy

Critical phenomena of a nuclear system in alternative ZM models

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Nuclear Science and Techniques 2000年第3期11卷 173-178页

作者： SONG Hong-Qiu (research center of Nuclear Theory of National Laboratory Of Heavy Ion Accelerator of Lanzhou, Lanzhou 73.000 shanghai Institute of Nuclear research, the chinese academy of sciences, shanghai 201800) 兰州原子核理论研究中心重离子加速器国家实验室甘肃兰州 730000

Alternative versions of the ZM model are extended to asymmetric nuclear matter by including p meson degree of freedom in the Lagrangian. The extended models are then used to study the thermodynamical properties of asymmetric nuclear matter at finite temperature. The critical temperature for a liquid- gas phase transition in nuclear matter and its dependence on asymmetry parameter are calculated. The limiting temperature Tlim, which reflects Coulomb instability of hot nuclei, is studied. The calculated results are compared with that given by the original ZM model.

关键词：放射性核素临界现象 ZM模型固气相变热力学稳定性核子核反应临界温度

Semantic Search among Heterogeneous Biological Databases Based on Gene Ontology

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 365-370页

作者： Shun-Liang CAO, Lei QIN1, Wei-Zhong HE1, Yang ZHONG2, Yang-Yong ZHU, and Yi-Xue LI1,3. Department of Computing and Information Technology, Fudan University, shanghai 20043., china shanghai {3. for Bioinformation 1 Technology, shanghai 20023., china School of Life sciences, Fudan University, shanghai 20043., china Bioinformation {3. of shanghai Institutes for Biological sciences, the chinese academy of sciences, shanghai 20003., china Department of Computing and Information Technology Fudan University Shanghai 200433 China shanghai center for Bioinformation Technology shanghai 20023. China School of Life sciences Fudan University Shanghai 200433 China Bioinformation center of shanghai Institutes for Biological sciences the Chinese Academy of Sciences Shanghai 200031 China

Semantic search is a key issue in integration of heterogeneous biological databases. In this paper, we present a methodology for implementing semantic search in BioDW, an integrated biological data warehouse. Two tables are presented: the DB2GO table to correlate Gene Ontology (GO) annotated entries from BioDW data sources with GO, and the semantic similarity table to record similarity scores derived from any pair of GO terms. Based on the two tables, multifarious ways for semantic search are provided and the corresponding entries in heterogeneous biological databases in semantic terms can be expediently searched.

关键词： Gene Ontology semantic search BioDW