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The Effect of Bovine IFN-α on the Immune Response in Guinea Pigs Vaccinated with DNA Vaccine of Foot-and-Mouth Disease Virus

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 701-706页

作者： Hui-Chen GUO, Zai-Xin LIU*, Shi-Qi SUN, Qing-Wen LENG1, Dong LI, Xiang-Tao LIU, and Qing-Ge XIE* key laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 73.046, china College of Animal Science and Technology, Shihezi {3., Shihezi 83.003. china 1 key laboratory of Animal Virology of Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences College of Animal Science and Technology Shihezi University

In this study, we constructed recombinant plasmid pcDNA3.1/P12X3.3. including P1, 2A, 3., 3. and part of 2B gene of FMDV and pcDNA3.1/IFN containing the gene encoding bovine IFN-α. We inoculated the DNA vaccine pcDNA3.1/P12X3.3. with or without pcDNA3.1/IFN to evaluate the effi- ciency of this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine, all of guinea pigs were challenged with 103.ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T lymphocyte proliferation response was tested by MTT assay. The result shows that guinea pigs inoculated by pcDNA3.1/P12X3.3. alone or with pcDNA3.1/IFN generated specific antibodies and induced an FMDV-specific T lymphocyte proliferation response. FMDV challenge tests showed that one in four guinea pigs immunized by pcDNA3.1/ P12X3.3. with pcDNA3.1/IFN was protected from the FMDV serotype O infection. This result indicated that the efficiency of the DNA vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate was considerably lower than that immunized with conventional FMD vaccine.

关键词： foot-and-mouth disease virus (FMDV) DNA vaccine IFN-α gene vaccination

Study of Synthesis Identification in Cutting Process with Fuzzy Neural Network

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厦门大学学报（自然科学版） 2002年第S1期41卷 40-41页

作者： LIN Bin, YU Si-yuan, ZHU Hong-tao, ZHU Meng-zhou, LIN Meng-xia (The state Education Ministry key laboratory of High Temperature Structure Ceramics and Machining Technology of Engineering Ceramics, Tianjin {3., Tianjin 3.0072, china)

With the development of industrial production modernization, FMS and CIMS will become more and more popularized. For its control system is increasingly modeled, intellectualized and automatized, in order to raise the reliability and stability in the manufacturing process, the comprehensive monitoring and diagnosis aimed at cutting tool wear and chatter become more and more important and get rapid development. The paper tried to discuss of the intellectual status identification method based on acoustics-vibra characteristics of machining process, and propose that the working conditions may be taken as a core, complex fuzzy inference neural network model based on artificial neural network theory, and by using various kinds of modernized signal processing method to abstract enough characteristics parameters which will reflect overall processing status from machining acoustics-vibra signal as information source, to identify different working condition, and provide guarantee for automation and intelligence in machining process. The complex network is composed of NNw and NNs, Each of them is composed of BP model network, NNw is weight network at rule condition, NNs is decision-making network of each status. Y out is final inference result which is to take subordinate degree as weight from NNw, to weight reflecting result from NNs and obtain status inference of monitoring system. In the process of machining, the acoustics-vibor signal were gotten by the acoustimeter and the acceleration piezoelectricity detector, the date is analysed by the signal processing software in time and frequency domain, then form multi feature parameter vector of criterion pattern samples for the different stage of cutting chatter and acoustics-vibra multi feature parameter vector. The vector can give a accurate and comprehensive description for the cutting process, and have the characteristic which are speediness of time domain and veracity of frequency domain. The research works have been pract

关键词： artificial neural network, synthesis identification, fuzzy inference on-line monitoring, acoustics-vibra signal

The Research on Cryptographic Schemes

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ZTE Communications 2007年第4期5卷 1-5页

作者： Zheng Shihui (Information Security Center of state key laboratory of Networking and Switching Technology, Beijing {3. of Posts and Telecommunications, Beijing 100876, china)

Cryptography is an important technology for information security. It mainly includes symmetric and asymmetric cryptographic algorithms and protocols. For the symmetric cryptographic algorithms, it is easy to deduce decryption keys from the encryption keys and vice versa. Because this algorithm encrypts and decrypts data very quickly, it is applicable in situations where large numbers of data have to be protected. However, for the asymmetric algorithm, extracting the secret key from the public key is computationally infeasible. Although the performance speed of the asymmetric algorithm is much slower than that of the symmetric algorithm, the asymmetric algorithm has key distribution and management advantages over the symmetric one. Moreover, it is a perfect digital signature scheme.

关键词： Hash The Research on Cryptographic Schemes

Dissociation behavior of (CH_4+C_2H_4) hydrate in the presence of sodium dodecyl sulfate

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Petroleum Science 2010年第1期7卷 112-117页

作者： Peng Baozi Sun Changyu Chen Guangjin Tang Xulong Liu Peng Chen Jun Zhang Yanqin state key laboratory of Heavy Oil Processing China University of Petroleum Beijing 102249 China High Pressure Fluid Phase Behavior ＆ Property Research laboratory China University of Petroleum Beijing 102249 China

Separation of a mixture of CH4＋C2H4 gas by forming hydrate in ethylene production has become of interest, and the dissociation behavior of （CH4＋C2H4） hydrate is of great importance for this process. The hydrate formation rate could be increased by adding a small amount of sodium dodecyl sulfate （SDS） into water. In this work, the kinetic data of CH4（18.5 mol%）＋C2H4（81.5 tool%） hydrate decomposition in the presence of 1000 mg.L-~ SDS at different temperatures and pressures were measured with the depressurizing method using a sapphire cell apparatus. The experimental results show that the dissociation rate of （CH4＋C2H4） mixed gas hydrate was large at high temperature and low pressure, and temperature had great influence on the dissociation rate at high pressure. A kinetic model for hydrate dissociation was proposed and the calculated results from the kinetic model were in good agreement with the experimental data. The activation energy for dissociation of the （CH4＋C2H4） mixed gas hydrate was determined to be 43.9 kJ-mol-~, which is lower than that of methane （81.0 ***-~） and ethane （104.0 kJ＇mol-~） hydrate in water （Clarke and Bishnoi, 2004）. As the similar molecular dimensions between ethane and ethylene, the close activation energy need to be provided for ethylene hydrate dissociation. The obtained activation energy of the （CH4＋C2H4） mixed gas hydrate dissociation indicates that the dissociation of the （CH4＋C2H4） mixed gas hydrate is easier than that of pure CH4 or C2H4 gas hydrate.

关键词： Methane ethylene hydrate dissociation SDS

Blue-shift of Nano-Y_2O_3.: Eu^(3.) Emission Spectra Excited by X-ray

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Journal of Materials Science & Technology 1997年第4期13卷 351-353页

作者： Qiang LI Lian GAO and Dongsheng YAN(state key Lab. of High Performance Ceramics & Superfine Microstructure, Shanghai Institute of Ceramics,Chinese Academy of Sciences, Shanghai 200050, china) state key Lab. of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai 200050 China

Nano-Y_2O_3.Eu^(3.) powder was prepared by the homogeneous precipitation. With controlling the conditions of the reaction, nano powders with different grain size were obtained. It is found that the blue-shift phenomena exist in the nano-Y2O3.Eu3. emission spectra excited by X-ray. The wave lengths of the peak (5D0→7F2) are related with the grain size of the powder

关键词： Eu Nano Emission Spectra Excited by X-ray Blue-shift of Nano-Y2O3

Experiments on Development of Channel Pattern in the Process of River Reformation

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四川大学学报（工程科学版） 2005年第S1期37卷 128-132页

作者： ZHANG Junyong CHEN Li LIU Yucheng DING Zhiliang(state key Lab.of Water Resources and Hydropower Eng.Sci.,Wuhan Univ.,Wuhan 43.072,china) Lab. of Water Resources and Hydropower Eng. Sci. Wuhan Univ. Wuhan 430072 China

The changing of flow conditions caused by construction of hydro-projects will result in river's refor- mation and development of channel *** this paper,the development of channel pattern in the process of reformation is studied by means of flume *** of slope,grain size,angle-of-inflow,dis- charge and discharge process are comprehensively analyzed according to the experimental *** was found that the channel would be widened as the river slope or discharge increased and grain s...

关键词： reformation process channel pattern angle-of-inflow discharge process

Impact of copy number of distinct SV40PolyA segments on expression of a GFP reporter gene

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Science china(Life Sciences) 2010年第5期53卷 606-612页

作者： YIN Kang,WANG XiuFang,MA Huan,XIE Ying,FENG JingJing,YANG QinQing & Lü ZhanJun Hebei key Lab of laboratory Animal,Department of Genetics,Hebei Medical university,Shijiazhuang 050017,{3. 1. Hebei key Lab of laboratory Animal Department of Genetics Hebei Medical University Shijiazhuang 050017 China

The presence of Alu repeats downregulates the expression of the green fluorescent protein(GFP) *** found that SV40PolyA(PolyA,240 bp),in either orientation,eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu *** this study,4 different segments(each 60 bp) were amplified from antisense PolyA(PolyAas) by PCR,and inserted upstream of Alu14 in pAlu14 plasmid(14 Alu repeats inserted downstream of the GFP gene in vector pEGFP-C1 in a head-tail tandem manner).Segments 1F1R(the first 60 bp segment at the 5' end of PolyAas) and 4F4R(the fourth 60 bp segment from the 5' end of PolyAas) did not activate GFP gene expression,whereas 2F2R and 3.3.(the middle two segments) did(as detected by Northern blot analysis and fluorescent microscopy).Different copy numbers of 2F2R and 3.3. segments,in a head and tail tandem manner,were inserted downstream of the GFP gene in pAlu14.p2F2R*4-Alu28,p3.3.*4-Alu18 and p3.3.*4-Alu28 were used as length controls to verify that the decrease in the expression of GFP was not due to the increased length of the inserted segment in the expression *** found that 2 and 4 copies of 2F2R or 3.3. activated the GFP gene more strongly than one copy of ***,more than 8 copies of 2F2R or 3.3. reduced the activation of the GFP *** concluded that SV40PolyAas contained at least two gene-activating elements(2F2R and 3.3.) and 2-4 copies of 2F2R or 3.3. were optimal for the expression of the GFP gene.

关键词： copy number SV40PolyA repeat sequence GFP Alu

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高温超导氧化物正常态输运性质综述

物理学进展 1990年第4期10卷 472-502页

作者：邢定钰刘楣南京大学固体微结构物理实验室和理论物理中心东南大学物理系

高温超导氧化物正常态的输运性质有很强的各向异性和异常的温度效应。本文综述了正常态电阻率。

关键词：超导体氧化物正常态输运性质

Synthesis　and　Diffuse　Reflectance　Spectrum　of　Open－framework　Cobalt　Phosphate　DAF－2　From　Alcoholic　System

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Chemical Research in Chinese Universities 1996年第4期12卷 390-392页

作者： YU Ji-hong CHEN Jie-sheng and XU Ru-ren(key laboratory of Inorganic Hydrothermal Synthesis,Department of Chemistry,Jilin university,Changchun,13.023. JILIN UNIV DEPT CHEMKEY LAB INORGAN HYDROTHERMAL SYNTHCHANGCHUN 130023PEOPLES R CHINA

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关键词： s:Synthesis Cobalt phosphate Alcoholic system Diffuse reflectance Spectroscopy