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Advances of multiplex and high throughput biomolecular detection technologies based on encoding microparticles

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Science china Chemistry 2011年第8期54卷 1185-1201页

作者： ZHAO XiangWei, ZHAO YuanJin & GU ZhongZe state key laboratory of {3. southeast university, nanjing 210096, china 1. state key laboratory of {3. Southeast University Nanjing 210096 China

The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.

关键词： multiplex bioassays multiplex labels encoded microcarrier binding assays high throughput screening

Review for dynamic researches in civil engineering in recent years

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Science china(Technological Sciences) 2010年第5期53卷 1450-1452页

作者： XU ZhaoDong RC&PC key laboratory of Education Ministry,southeast {3.,nanjing 210096,china 1. RC&PC key laboratory of Education Ministry Southeast University Nanjing 210096 China

Structure dynamic research is a hot field in civil *** involves in many challenge topics,such as dynamic analysis and tests under earthquake,wind or other dynamic *** paper introduces main dynamic researches in civil engineering in recent years,which will be classified into five aspects,especially for researches published in Science in china Series E:Technological Sciences.

关键词： civil engineering structures dynamic researches

Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons

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Science china(Information Sciences) 2011年第10期54卷 2199-2208页

作者： PAN HaiXian 1,L XiaoYing 1 ,WANG ZhiGong 2,REN TianLing 3.FANG Tao 1,ZHANG Jie 1,ZHOU ChangJian 3.& WANG LiGang 3.1 state key laboratory of {3.,southeast university,nanjing 210096,china 2 Institute of RF-& OE-ICs,southeast university,nanjing 210096,china 3.Institute of Microelectronics,Tsinghua university,Beijing 100084,china 1. state key laboratory of {3. Southeast University Nanjing 210096 China2. Institute of RF-& OE-ICs Southeast University Nanjing 210096 China3. Institute of Microelectronics Tsinghua University Beijing 100084 China

Microelectrode arrays (MEAs) for stimulation and signal recording of in vitro cultured neurons are *** MEA is composed of 60 independent electrodes with 59 working ones and one reference *** electrodes are divided into 3. *** each pair of electrodes,four independent states can be realized to define the accessing modes of neurons cultured on the surface of the electrodes.A total MEA covers an area of 10 mm×10 *** are fabricated in a silicon-based semiconductor *** implemented MEA is bonded on a specially designed printed-circuit-board (PCB) and surrounded by a culture *** impedance measurement has been made to verify the electrical characteristics of *** surface was modified to enhance the biocompatibility.A series of PC12 cells culture experiments validates the effectiveness of the *** extracellular signal recording experiment with acetylcholine (Ach) as a stimulant has been carried out,and the results show the feasibility of MEAs for extracellular action potential *** electrical stimulation and recording experiments have been carried out *** indicate that MEAs can be used for extracellular stimulation,recording,simultaneous stimulation and recording,and isolation of PC12 cells network cultured in vitro.

关键词： microelectrode arrays (MEAs) extracellular recording stimulation and recording neurons net-work in vitro

A research into the large-scaled spatial forms of cities based on urban design

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Science china(Technological Sciences) 2009年第9期52卷 2486-2496页

作者： WANG JianGuo School of Architecture, key laboratory of Urban and Architectural Heritage Conservation of Ministry of Education, southeast university,{3., china 1. School of Architecture Key laboratory of Urban and Architectural Heritage Conservation of Ministry of Education Southeast University Nanjing 210096 China

In the process of unprecedented urbanization in contemporary china, a great many of Chinese cities have witnessed a rapid expansion of urban scale to different degrees. With the continuous compilation, amendment and enforcement of urban planning, the functional structure, spatial environment, street patterns and textures and even the social aspect of cities have changed conspicuously, all of which have directly happened to the spatial forms of the dimension, or even the multi-dimension of a certain city. By describing and analyzing the structure, evolution and explicit tokens of the spatial forms of cities, this thesis attempts to set up a model of factors that affect urban forms, as well as discuss the attributes, protection, adjustment and development-potential of urban land closely associated with forms. In addition, on the basis of the professional features for urban design to participate in the modeling and construction-management of desirable spatial forms of cities, the author has put forward technical measures for quantitatively appraising and analyzing the large-scaled spatial forms of cities, thus offering technical support to municipal governments and management departments of planned construction.

关键词： large-scaled spatial forms of cities urban design digital technology

In situ synthesis of DNA micro-arrays using typography technique

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Science china Chemistry 2007年第1期50卷 75-83页

作者： TANG JianXin 1,2 ,HE NongYue 1,2,3.&LI Song 1 1state key laboratory of {3.,southeast university,nanjing 210096,china 2key laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province,Hunan university of Tech- nology,Zhuzhou 412008,china 3.hongshan Huoju Polytechnic,Zhongshan 52843.,china 1. state key laboratory of {3. Southeast University Nanjing 210096 China 2. key laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province Hunan University of Technology Zhuzhou 412008 China 3. Zhongshan Huoju Polytechnic Zhongshan 528436 China

A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide,which has the celerity,high spatial resolution,lower cost,reliable operation,and high synthetic *** principle and process of the typography technique for fabricating gene-chips have been described in detail.A suit of poly(terafluoroethylene)devices for synthesizing oligonucleotide arrays were designed and prepared,and the fiber tubes with a number of nano-or micron-channels were em- *** oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography *** four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent *** was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms(SNP)and to detect mutations.

关键词： typography oligonucleotide in situ synthesis DNA microarrays

Three-dimensional subwavelength components utilizing THz surface plasmons

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Science china(Information Sciences) 2012年第1期55卷 79-89页

作者： ZHOU YongJin,JIANG Quan&CUI TieJun state key laboratory of Millimeter Waves,School of Information Science and Engineering,{3. university,nanjing 210096,china 1. state key laboratory of Millimeter Waves School of Information Science and Engineering Southeast University Nanjing 210096 China

Since surface plasmons(SPs)are not constrained by the diraction limit,they have important potential applications in the optical,terahertz(THz),and microwave *** this paper,we firstly present a review of three-dimensional(3.) bidirectional and multi-directional THz SP splitters based on the rectangular metallic groove gratings with finite thickness,in which a metallic wire was used to excite the THz *** experimental verifications of such splitters have been implemented in the microwave frequencies,and the measurement results have excellent agreements to the full-wave *** improve the performance,a 3. bidirectional THz SP splitter with the transverse confinement and a 3. bidirectional bending THz SP splitter are then proposed,which have shown very good splitting performance,compact sizes,and better transverse confinement of EM ***,a broadband slow-wave system of subwavelength thickness is reviewed and a new bending slow-wave system utilizing THz SPs is *** and simulations in microwave frequencies have good agreements,showing the validity of these components.

关键词： surface plasmons surface waves terahertz slow wave splitter

Study on correlations of modal frequencies and environmental factors for a suspension bridge based on improved neural networks

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Science china(Technological Sciences) 2010年第9期53卷 2501-2509页

作者： DING YouLiang,DENG Yang & LI AiQun key laboratory of Concrete and Prestressed Concrete Structure of Ministry of Education,southeast {3.,nanjing 210096,china 1. key laboratory of Concrete and Prestressed Concrete Structure of Ministry of Education Southeast University Nanjing 210096 China

By using of long-term monitoring data of Runyang Suspension Bridge,the improved back-propagation neural networks (BPNNs) are formulated for modeling the correlations between modal frequencies and environmental conditions including wind,temperature and vehicle ***,with the correlation models the environmental effects on modal frequencies are quantified and the abnormal changes of measured frequencies are detected by means of the hypothesis *** results reveal that BPNN-based correlation models improved by both early stopping and Bayesian regularization techniques exhibit excellent generalization *** the developed correlation models can effectively reduce the environmental variability in modal *** t-test method provides a good capability to detect the damage-induced 0.16% and 0.12% abnormal changes of the 5th and 6th modal frequencies,***,the proposed method is suitable for real-time monitoring of suspension bridge conditions.

关键词： modal frequency environmental condition correlation artificial neural networks hypothesis test suspension bridge

Heuristic for no-wait flow shops with makespan minimization based on total idle-time increments

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Science in china(Series F) 2008年第7期51卷 896-909页

作者： LI XiaoPing1,2 & WU Cheng3.1 School of Computer Science & Engineering, southeast university, nanjing 210096, china 2 {3. laboratory of Computer Network and Information Integration (southeast university), Ministry of Education, nanjing 210096, china 3.Department of Automation, Tsinghua university, Beijing 100084, china 1. School of Computer Science & Engineering Southeast University Nanjing 210096 China 2. key laboratory of Computer Network and Information Integration (southeast {3.) Ministry of Education Nanjing 210096 China 3. Department of Automation Tsinghua University Beijing 100084 China

No-wait flow shops with makespan minimization are classified as NP-hard. In this paper, the optimization objective is equivalently transformed to total idle-time minimization. The independence relationship between tasks is analyzed, and objective increment properties are established for the fundamental operators of the heuristics. The quality of the new schedules generated during a heuristic is judged only by objective increments and not by the traditional method, which computes and compares the objective of a whole schedule. Based on objective increments, the time complexity of the heuristic can be decreased by one order. A seed phase is presented to generate an initial solution according to the transformed objective. Construction and improvement phases are introduced by experimental analysis. The FCH （fast composite heuristic） is proposed and compared with the most effective algorithms currently available for the considered problem. Experimental results show that the effectiveness of the FCH is similar to that of the best methods but requires far less computation time. The FCH can also be efficient in real time scheduling and rescheduling for no-wait flow shops.

关键词： no-wait flow shops heuristic makespan Tabu search

A decision-making model of development intensity based on similarity relationship between land attributes intervened by urban design

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Science china(Technological Sciences) 2010年第7期53卷 1743-1754页

作者： WANG JianGuo1, ZHANG Yu2 & FENG Han3.1 School of Architecture, southeast university key laboratory for Urban and Architectural Heritage Conservation, Ministry of Education,{3., china 2 School of Architecture, southeast university,{3., china 3.Faculty of Architecture, Delft university of Technology, 2628 CR Delft, Netherlands 1. key laboratory for Urban and Architectural Heritage Conservation Ministry of Education Nanjing 210096 China2. School of Architecture Southeast University Nanjing 210096 China3. Faculty of Architecture Delft University of Technology 2628 CR Delft Netherlands

The paper presents a dynamic model intervened by urban design for the decision-making of land development intensity, which expresses the inherent interaction mechanism between lands based on the evaluation of land attributes and their similarity relationship. Each land unit is described with several factors according to their condition and potential for development, such as land function, accessibility, historical site control, landscape control, and so on. Then, the dynamic reference relationship between land units is established according to the similarity relationship between their factors. That means lands with similar conditions tend to have similar development intensities, which expresses the rule of the spontaneous urban development. Therefore, the development intensities of the pending lands can be calculated by the confirmed ones. Furthermore, the system can be actively intervened by adjusting the parameters according to urban design or planning intentions. And the reaction of the system offers effective support and reference for reasonable decision. The system with multiple intervention input is not only a credible tool for deriving development intensities, but also a platform to activate urban design conception. Above all, the system as a socio-technical tool integrates the optimization of form, function and environment, and embodies the principle of impersonality, justice and flexibility in the decision of land development intensity.

关键词： land development intensity urban design land attribute similarity relationship complex system FAR