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Raman Spectroscopic Analysis of the Effect of Ultraviolet Irradiation on Calf Thymus DNA

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 39-46页

作者： Yu-Long TANG Zhou-Yi GUO~* institute of Laser and Life science.south{3. Normal University,guangzhou 51063.,china institute of Laser and Life science South China Normal UniversityGuangzhou 510631 China

Raman spectroscopy was used for the first time to detect the effect of independent UVA(ultraviolet-A:3.0-400 nm)and UVB(ultraviolet-B:280-3.0 nm)irradiation on the calf thymus DNA inaqueous *** both UVA and UVB irradiation for 1 h or 3.h,the damage to the conformation of DNAwas rnoderate,but the reduction of the B-form DNA component was *** UVA and UVB causedsignificant damage to the deoxyribose moiety and bases,among which the pyrimidine base pairs were moreseriously *** appeared to be preferential damaging sites on DNA molecules caused by UVA andUVB irradiation,UVA irradiation caused more damage to the deoxyribose than UVB irradiation,while UVBirradiation caused more significant damage to the pyrimidine moiety than UVA *** UVB irradia-tion for 3.h,unstacking of the AT base pairs and the cytosine ring took place,severe damage to the thyminemoiety occurred,and some base pairs were ***,with either UVA or UVB irradiation for 3.h,the photoreactivation of DNA *** damage to the DNA caused by UVB was immediate,while thedamage caused by UVA was proportional to the irradiation *** experimental results partly indicatethe formation of some cyclobutane pyrimidine dimers and(6-4)pbotoproducts.

关键词： Raman spectroscopy deoxyribonucleic acid(DNA) ultraviolet irradiation(UV) base pair

RAZUMIKHIN-TYPE THEOREMS FOR ASYMPTOTIC STABILITY OF IMPULSIVE STOCHASTIC FUNCTIONAL DIFFERENTIAL SYSTEMS

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Journal of Systems science and Systems Engineering 2010年第1期19卷 72-84页

作者： Pei CHENG~1 Feiqi DENG~2 Xisheng DAI~3.Systems Engineering institute,south china University of Technology,{3. 510640,china Systems Engineering institute South China University of Technology Guangzhou China

In this paper, we investigate the pth moment uniformly asymptotic stability of impulsive stochastic ftmctional differential systems by extending some Razumikhin-type theorems. Based on the Lyapunov functions and Razumikhin techniques, some criteria are established and their applications to impulsive stochastic delay systems are proposed. An illustrative example shows the effectiveness of our results.

关键词： Stochastic functional differential systems Impulse Razumiltial theorems Asymptotic stability

The　photon　theory　hypothesis　about　photon　tunneling　microscope＇s　subwavelength　resolution

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光电子·激光 1996年第4期7卷 236-239+259页

作者： Yanbin Zhu Junfu Ma(Laser Li fe science institute, south{3. Normal University, guangzhou 51063.,P.R.china) 华南师范大学激光生命科学所广东广州

Ｔｈｅｐｈｏｔｏｎｔｈｅｏｒｙｈｙｐｏｔｈｅｓｉｓａｂｏｕｔｐｈｏｔｏｎｔｕｎｎｅｌｉｎｇｍｉｃｒｏｓｃｏｐｅ＇ｓｓｕｂｗａｖｅｌｅｎｇｔｈｒｅｓｏｌｕｔｉｏｎ￥ＹａｎｂｉｎＺｈｕ；ＪｕｎｆｕＭａ（ＬａｓｅｒＬｉｆｅ... 详细信息

Ｔｈｅｐｈｏｔｏｎｔｈｅｏｒｙｈｙｐｏｔｈｅｓｉｓａｂｏｕｔｐｈｏｔｏｎｔｕｎｎｅｌｉｎｇｍｉｃｒｏｓｃｏｐｅ＇ｓｓｕｂｗａｖｅｌｅｎｇｔｈｒｅｓｏｌｕｔｉｏｎ￥ＹａｎｂｉｎＺｈｕ；ＪｕｎｆｕＭａ（ＬａｓｅｒＬｉｆｅＳｃｉｅｎｃｅＩｎｓｔｉｔｕｔｅ，...

关键词： tunneling hypothesis microscope about resolution subwavelength theory The photon

Ecology of the rehabilitation of vegetation on tropical coastal eroded land in Guangdong， china

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Journal of envir.nmental sciences 1995年第1期7卷 74-84页

作者： Yu Zuoyue Wang Zhuhao south china institute of Botany, Chinese Academy of {3.s,guangzhou 510650,china south china Inst of Botany Chinese Academy of Science Guangzhou China

ＥｃｏｌｏｇｙｏｆｔｈｅｒｅｈａｂｉｌｉｔａｔｉｏｎｏｆｖｅｇｅｔａｔｉｏｎｏｎｔｒｏｐｉｃａｌｃｏａｓｔａｌｅｒｏｄｅｄｌａｎｄｉｎＧｕａｎｇｄｏｎｇ，ＣｈｉｎａＹｕＺｕｏｙｕｅ；ＷａｎｇＺｈｕｈａｏＳｏｕｔｈＣｈ... 详细信息

ＥｃｏｌｏｇｙｏｆｔｈｅｒｅｈａｂｉｌｉｔａｔｉｏｎｏｆｖｅｇｅｔａｔｉｏｎｏｎｔｒｏｐｉｃａｌｃｏａｓｔａｌｅｒｏｄｅｄｌａｎｄｉｎＧｕａｎｇｄｏｎｇ，ＣｈｉｎａＹｕＺｕｏｙｕｅ；ＷａｎｇＺｈｕｈａｏＳｏｕｔｈＣｈｉｎａＩｎｓｔｉｔｕｔｅｏｆＢｏｔ...

关键词： tropics ereded land ecology of vegetation recovery.

Planning envir.nmental Impact Assessment Orienting Sustainable Development: Opportunities and Challenges

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Chinese Journal of Population,Resources and environment 2005年第4期3卷 33-39页

作者： Sun Yanjun 1, Chen Xingeng 1, Bao Yun 2, Peng Xiaochun 3.& Gao Changbo 1 1.School of envir.nmental science and Engineering, Sun Yat-sen University, guangzhou 510275,{3. 2. Research Center for Coastal Ocean science and Technology, Sun Yat-sen University, guangzhou 510275,{3. 3. south china institute of envir.nmental science, sep., guangzhou 510655,{3.

Strategic envir.nmental Assessment is a frontier subject in the field of envir.nmental Impact Assessment. In the past two decades, especially in recent years, much more importance has been attached to Strategic envir.nmental Assessment. The envir.nmental Impact Assessment Law of the P.R. china which was promulgated provides a great opportunity for the development of Planning envir.nmental Impact Assessment and brings great challenges for the development of traditional Project envir.nmental Impact Assessment and Planning envir.nmental Impact Assessment at the same time. In order to promote the implementation of "The EIA Law", the inherent limitations of Project envir.nmental Impact Assessment must be identified sufficiently and the theory research and practice of Planning envir.nmental Impact Assessment should be strengthened as well. Measures should be taken currently to enforce the operation system. The authors wish to offer a few references to the progress and implementation of Planning envir.nmental Impact Assessment in china.

关键词： planning envir.nmental impact assessment strategic envir.nmental assessment sustainable development opportunities challenges.

Discovery of seep carbonate nodules as new evidence for gas venting on the northern continental slope of south china Sea

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Chinese science Bulletin 2006年第10期51卷 1228-1237页

作者： CHEN Zhong1,2,3. YAN Wen1,2,3. CHEN Muhong1,3. WANG Shuhong1,2,3. LU Jun1,3. ZHANG Fan1,3. XIANG Rong1,3. XIAO Shangbin1,3. YAN Pin1,2,3.& GU Senchang1,2,3.1. south china Sea institute of Oceanology, Chinese Academy of Sci-ences, {3. 5103.1, china 2. {3. Center for Gas Hydrate Research, Chinese Academy of sciences, {3. 5103.1, china 3. Key Laboratory of Marginal Sea Geology, south china Sea institute of Oceanology and {3. institute of Geochemistry, Chinese Academy of sciences, {3. 5103.1, china south china Sea institute of Oceanology Chinese Academy of Sciences Guangzhou China guangzhou Center for Gas Hydrate Research Chinese Academy of Sciences Guangzhou China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology and Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China

Seep carbonate nodules were firstlycollected from the southwestern Dongsha area onnorthern continental slope of south china Sea formineralogical and geochemical studies. The resultsof X-ray diffraction show that carbonate nodules arecomposed of Fe-rich domolite,siderite and a few cal-cite and aragonite, as well as a small quantity ofnon-carbonate minerals such as goethite, quartz andclay minerals. Fe-rich dolomite and siderite charac-terized by euhedral microcrystalline structure weredirectly precipitated at cold seeps. The δ 13. valuesfor Fe-rich dolomite and siderite in the bulk nodulesvary from ?18.24‰ to ?3..07‰, and the δ 18O valuesrange from 0.42 to 2.76‰. Their moderate depleted13. reflects that carbon origin is possibly thermoge- netic gas or mixed gas, which is evidence of gasventings in the seafloor. Moreover, massive wormtube fossils related to cold seeps were found on thesurfaces of carbonate nodules, and the conduitsand/or channels in semi-solidified nodules whichwere not filled by carbonate or sediment were alsoobserved. A preliminary conclusion is that activemicro gas venting with a conduit/channel diameterrange from 200 μm to 600 μm possibly exists inmodern seafloor of the carbonate nodule area.

关键词：渗出碳酸盐节碳氧同位素甲烷渗漏天然气水化物

Fluorescence Resonance Energy Transfer Analysis of Bid Activation in Living Cells during Ultraviolet-induced Apoptosis

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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 37-45页

作者： Yinyuan WU Da XING Lei LIU Tongsheng CHEN and Wei R.CHEN MOE Key Laboratory of Laser Life science &institute of Life science,{3. china Normal University,guangzhou 51063.,china MOE Key Laboratory of Laser Life science & institute of Laser Life science South China Normal UniversityGuangzhou 510631 China

Ultraviolet (UV) irradiation is a DNA-damaging agent that triggers apoptosis through both themembrane death receptor and mitochondrial apoptotic signaling ***,a pro-apoptotic Bcl-2family member,is important in most cell types to apoptosis in response to DNA *** this study,arecombinant plasmid,YFP-Bid-CFP,comprised of yellow and cyan fluorescent protein and a full length Bid,was used as a fluorescence resonance energy transfer analysis (FRET) *** the FRET techniquebased on YFP-Bid-CFP,we found that Bid activation was initiated at 9±1 h after UV irradiation,and theaverage duration of the activation was 75±10 *** activation coincided with a collapse of the mitochondrialmembrane potential with an average duration of 50±10 min. When cells were pretreated with Z-IETD-fmk(caspase-8 specific inhibitor) the process of Bid activation was completely inhibited,but the apoptosis wasonly partially affected.Z-DEVD-fmk (caspase-3.inhibitor) and Z-FA-fmk (non asp specific inhibitor) didnot block Bid ***,the endogenous Bid activation with or without Z-IETD-fmk in responseto UV irradiation was confirmed by Western *** summary, using the FRET technique,we observedthe dynamics of Bid activation during UV-induced apoptosis and found that it was a caspase-8 dependentevent.

关键词： ultraviolet irradiation Bid activation apoptosis caspase-8 fluorescence resonance energy transfer analysis

Impacts of Typhoons Tianying and Dawei on seagrass distribution in Xincun Bay,Hainan Province,china

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Acta Oceanologica Sinica 2011年第1期30卷 32-39页

作者： YANG Dingtian HUANG Daojian CAS key Laboratory of Tropical Marine envir.nmental Dynamics South China Sea Instituteof Oceanology Chinese Academy of Sciences Guangzhou 510301 China south china institute of {3. sciences Ministry of Environmental Protection of thePeople＇s Republic of China Guangzhou 510655 China

More than 50% of the typhoons landing in china have landed on the southeast coast, where they have caused great pressure on the coastal environment. Seagrass, one of the most important con- stituents of coastal ecosystems, is also greatly affected by typhoons. In order to clarify how seagrass distribution variation is affected by typhoons in coastal areas in southeast china, data of Typhoons Dawei and Tianying （category 4 and category 2 respectively, which just ran through the southern part of Hainan province） have been studied. In situ observation and satellite remote sensing data （CBERS-china Brazil Earth Resources Satellite） in 2004 and 2006 were used to retrieve seagrass distribution in Xincun Bay, Hainan province. In situ observations showed that leaf length, stem biomass and above ground biomass on average showed evidence of reduction after Typhoons Tiany- ing and Dawei. However, seagrass density showed no evidence of reduction after typhoon Tianying and Dawei passed by and increased rapidly in January 2006. From results of satellite remote sensing data, seagrass distribution can be detected with high accuracy, and the area of seagrass distribution on the south coast of Xincun Bay in 2006 after the typhoon passed by was smaller than that in 2004 in region A and B. However, in region C, area of seagrass coverage under 20% increased. These results demonstrated that typhoons Tianying and Dawei damaged seagrass bed and helped seagrass to get rid of aged and dead leaves, and this correspondingly facilitated seagrass growth.

关键词： seagrass Typhoon Tianying and Dawei Xincun Bay

STUDIES ON REVERSE OSMOSIS sep.RATION OF AQUEOUS ORGANIC SOLUTIONS BY PAA/PSF COMPOSITE MEMBRANE

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Chinese Journal of Polymer science 2000年第2期18卷 115-122页

作者： Jun Fang De-min Jia Ji-cai Huang Qun-hui Guo Feng-lian Wu south-china University of Technology, guangzhou 510641,{3. guangzhou institute of Chemistry, Chinese Academy of sciences, guangzhou 510650,{3. 华南理工大学广东广州中科院广州化学所广东广州

The reverse osmosis (RO) sep.ration of aqueous organic solutions, such as alcohols, amines, aldehydes, acids, ketones, and esters etc., by PAA (polyacrylic acid)/PSF (polysulfone) composite membrane has been studied. It was found that the sep.ration results for aliphatic alcohols, amines and aldehydes are satisfactory, the solute rejection (R-a) and the volume fluxes of solutions (J(V)) for 1000 ppm ethanol, ethylamine and ethyl aldehyde are 66.2%, 61.0%, 84.0% and 0.90 x 10(-6), 0.3. x 10(-6), 0.40 X 10(-6) m(3./m(2) . s, respectively, at 5.0 MPa and 3. degrees C. R-a increased with increasing molecular weights of alcohols, amines and aldehydes, and the R-a for n-amyl alcohol, n-butylamine and n-butyl aldehyde reached 94.3., 88.6% and 96.0%, respectively. Satisfactory sep.ration results (R-a > 70%) for ketones, esters, phenols and polyols have been obtained with the PAA/PSF composite membrane. The effect of operating pressure on the properties of reverse osmosis has also been investigated. Analysis of experimental data with Spiegler-Kedem's transport model has been carried out and the membrane constants such as reflection coefficient sigma, solute and hydraulic permeabilities omega and L-p for several organic solutes have been obtained.

关键词： PAA/PSF composite membrane reverse osmosis sep.ration of aqueous organic solution