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培养因素对龙牙楤木体细胞胚胎发生的影响

四川大学学报（自然科学版） 2004年第6期41卷 1259-1263页

作者：李福安李建民李喜文王明宁邢苗青海医学院中医系西宁810001 青海师范大学生命科学系西宁810008 东北师范大学遗传与细胞研究所长春130024 深圳大学广东深圳518060

对龙牙楤木Araliaelate(Miq.)Seen幼芽体细胞胚胎发生过程中培养基的种类、2,4 D、BA、蔗糖浓度及活性碳等五个培养因素的影响进行了比较研究.结果表明,诱导体细胞胚胎发生的适宜基本培养基为MS培养基;蔗糖浓度从2%提高到3.诱导率逐渐下... 详细信息

对龙牙楤木Araliaelate(Miq.)Seen幼芽体细胞胚胎发生过程中培养基的种类、2,4 D、BA、蔗糖浓度及活性碳等五个培养因素的影响进行了比较研究.结果表明,诱导体细胞胚胎发生的适宜基本培养基为MS培养基;蔗糖浓度从2%提高到3.诱导率逐渐下降;当2,4 D/BA比值低时,有利于体细胞胚胎发生频率的提高,但不利于胚状体的产生;当该比值高时,必须添加活性碳才有利于提高体细胞胚胎发生的数量和质量.

关键词：龙牙楤木组织培养培养因素体细胞胚胎发生

Theoretical and experimental study of surface texturing with laser machining

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Advances in Manufacturing 2021年第4期9卷 538-557页

作者： Ya-Zhou Mao Jian-xi Yang Jin-Chen Ji School of Mechatronics Engineering Henan University of Science and TechnologyLuoyang 471003HenanPeople’s Republic of China School of Mechanical and Mechatronic Engineering University of Technology SydneySydneyNSW 2007Australia

To explore the forming process and mechanism of the surface texture of laser micropits,this paper presents the thermal model of laser machining based on the Neumann boundary conditions and an investigation on the effects of various parameters on the *** surface profile and quality of the formed micropits were analyzed using NanoFocus 3. equipment through a design of experiment(DOE).The results showed that more intense melting and splashing occurred with higher power density and narrower pulse ***,the compressive stress is an important indicator of the damage effects,and the circumferential thermal stress is the primary factor influencing the diameter *** the process of laser machining,not only did o{1.des such as CuO and ZnO generate,the energy distribution also tended to decrease gradually from region#1.to region#3.based on an energy dispersive spectrometer(EDS)*** factors significantly affecting the surface quality of the micropit surface texture are the energy and pulse *** relationship between taper angle and energy is appropriately *** on the formation process and mechanism of the surface texture of laser micropits provides important guidance for precision machining.

关键词： Laser machining Thermal stress damage Surface texturing Design of experiment(DOE) Micropit

Caveolin-1.Gene Silence Stimulate Expression of ERα and the Metastasis in Human Mammary Epithelial MCF1.A

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Caveolin-1 Gene Silence Stimulate Expression of ERα and the...

薄荷醇对低压低氧诱导小鼠肺动脉高压的作用及机制研究

“基因、进化与生理功能多样性”海内外学术研讨会暨中国生理学会第七届比较生理学学术会议

作者： Yang Wang~1.Shuang Feng~1.xi Wang~1.Chao Han~1.Zhao-Yi Wang~{1.Wei Zou~(1.2*) 1.College of Life Science,Liaoning Normal University,Dalian 1.6029 2 The Key Laboratory of Biotechnology and Molecular Pharmacy in Liaoning Province,Dalian 1.6029 {1.Cancer Center,Creighton University,2500 California Plaza,Omaha,NE 681.8,USA

Breast cancer is a common malignant tumor,threatening the women's health *** of malignant tumor is the main cause of tumor related death,which is a complicated biologic *** activation of estrogen receptor alpha(ERα) expression is an early event in breast cancer *** evidence supports the existence of a plasma membrane *** proteins constitute important structural components of caveolae,participate in signal transduction,and Caveolin-1.could inhibit the anchorage-independent cell *** study the relationship between Caveolin-1.and ER in breast cancer,we transcripted the siRNA into normal epithelial cells(MCF1.A) to silence the expression of *** we examined the expression of Caveolin-1.ERαand associated proteins by Western *** results indicate that Caveolin-1.was silenced by 85%at 96h,whereas we found that the expression of protein ERα66 and ERα{1. was up-regulated. With the Caveolin-1.down-regulation, the phosphorylation of ERK1.2 was increased. These data shows that the down-reg- ulation of Caveolin-1.could activate MAP kinase, including the phosphorylation of ERK1. 2,which played critical roles in the control of cell proliferation,differentiation,homeostasis, and survival via the expression of ERαwhich is residing in the ***,monolayer culture of MCF1.A,MCF1.A-ST1. MCF1.A-7SD8 and MCF-7 were scrape wounded and allowed to grow for 24h,the distance traveled was measured as the distance between the wound edge and mirgrating *** we also obtained the evidence that migrant distance were difference in the four cell lines, 50%in MCF1.A-7SD8 and {1.%in MCF1.A-ST1.comparing to MCF-7,little in MCF1.A by wounded *** results indicate that Caveolin-1.could significantly mediate membrane-initiated estrogen signaling pathway, it played an important role in mammary tumorigensis and may effect on cell metastasis in *** evidences will greatly advance the progress in prevention

关键词： Caveolin-1 Estrogen receptor Metastasis Breast cancer

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中国药理学通报 2024年第1期40卷 62-69页

作者：王武帅胡陶杨耀何滢蓉杨曦段清华杜萱王强西南医科大学临床医学院心血管内科四川泸州646000 西部战区总医院心血管内科四川成都610083 西部战区总医院药剂科四川成都610083 成都医学院四川成都610500

目的探讨薄荷醇对低压低氧诱导小鼠肺动脉高压的作用和机制。方法健康雄性1.~1.周C57小鼠、瞬时受体电位通道M8基因敲除(TRPM8-/-)小鼠各40只,分为对照组、薄荷醇组、低压低氧组、低压低氧+薄荷醇组,超声测量肺动脉加速时间(PAT)和肺... 详细信息

目的探讨薄荷醇对低压低氧诱导小鼠肺动脉高压的作用和机制。方法健康雄性1.~1.周C57小鼠、瞬时受体电位通道M8基因敲除(TRPM8-/-)小鼠各40只,分为对照组、薄荷醇组、低压低氧组、低压低氧+薄荷醇组,超声测量肺动脉加速时间(PAT)和肺动脉射血时间(PET),右心导管测量右心室收缩压(RVSP),计算右心室肥厚指数(RVHI),观察肺小动脉重构(1.0μm)情况,Western blot检测Krüppel样因子4(KLF-4)和增殖细胞核抗原(PCNA)蛋白表达。肺动脉平滑肌细胞(PASMCs)经低氧(3.氧浓度)、低氧+薄荷醇(1.0μmol·L^(-1.)处理,测定增殖和迁移能力。结果薄荷醇处理野生型小鼠后,PAT和PAT/PET比值增加(P0.05)。薄荷醇干预PASMCs后增殖、迁移能力下降(P1.P<0.05)。结论薄荷醇可能通过TRPM8抑制PASMCs增殖、迁移,改善低压低氧诱导的小鼠肺血管重构和肺动脉高压,其机制可能与上调KLF4表达有关。

关键词：薄荷醇瞬时受体电位通道M8 Krüppel样因子4 低压低氧性肺动脉高压肺血管重构肺动脉平滑肌细胞

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Optimum condition for rapid growth of multi-walled carbon nanotube arrays by chemical vapor deposition

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Optimum condition for rapid growth of multi-walled carbon na...

第六届全国物理无机化学会议

作者： Yanbin Cui~(1.*),Hui Wang~2,Zhaoyu Ren~1.Jintao Bai~1.Mei Zhang~(3.*) 1.National Key Laboratory of photoelectric technology and Functional materials(Culture Base), National photoelectric technology and Functional Materials & Application International Cooperation Base,{1. of Photonics & Photon-Technology,Northwest University,xi’an 71.069,China 2 Key Laboratory of Synthetic and Natural Functional Molecule Chemistry(Ministry of Education), College of Chemistry & materials science,Northwest University,xi’an 71.069, China 3.Department of Industrial and Manufacturing Engineering,High-Performance Materials {1., FAMU-FSU College of Engineering,2525 Pottsdamer Street,Tallahassee,FL 3.3.0,USA

Spinnable vertically aligned multi-walled carbon nanotube(CNT)arrays were grown by chemical vapor deposition from CH/Ar using Fe catalyst supported on Si *** the aim of improving the yield of CNT arrays,the chemical vapor deposition(CVD)conditions were systematically *** spinnable CNT arrays can grow to 700μm in 1. *** initial growth rate of spinnable CNT arrays is around 500μm/min and the activation energy is determined to be 1.2 kJ/mol.

关键词： carbon nanotube array chemical vapor deposition spinnable

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中国女性血沉参考值与地理因素的关系

陕西师范大学学报（自然科学版） 1996年第3期24卷 83-87页

作者：葛淼董维张川民岳大鹏杨青生李晓玲陕西师范大学地理学系陕西师范大学计算机科学系西安医科大学生物物理教研室

为中国女性血沉参考值提供科学依据，研究了中国２１９个地区２０６９３例健康成年女性的温氏法血沉参考值与五项地理因素之间的关系，发现海拔高度是影响女性血沉参考值最主要的因素，随着海拨高度的增大，女性血沉参考值逐渐减小．用... 详细信息

为中国女性血沉参考值提供科学依据，研究了中国２１９个地区２０６９３例健康成年女性的温氏法血沉参考值与五项地理因素之间的关系，发现海拔高度是影响女性血沉参考值最主要的因素，随着海拨高度的增大，女性血沉参考值逐渐减小．用教学回归的方法推导出了５个一元回归方程和一个多元回归方程．如果知道了某个地区的地理因素，就可以运用回归方程估算这个地区的女性温氏法血沉参考值．把中国分为青藏区、西南区、西北区、东南区、华北区。

关键词：血沉参考值地理因素血流流变学中国女性

Pathogenesis of pancreatic encephalopathy in severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 134-140页

作者： Zhang, xi-Ping Tian, Hua Hangzhou First Peoples Hosp Dept Gen Surg Hangzhou 310006 Peoples R China Zhejiang Univ Sch Med Second Affiliated Hosp Dept Gen Surg Hangzhou 310003 Peoples R China

BACKGROUND: Pancreatic encephalopathy (PE) is a serious complication of severe acute pancreatitis (SAP). In recent years, more and more PE cases have been reported worldwide, and the onset PE in the early stage was regarded as a poor prognosis sign of SAP, but the pathogenesis of PE in SAP still has not been clarified in the past decade. The purpose of this review is to elucidate the possible pathogenesis of PE in SAP. DATA SOURCES: The English-language literature concerning PE in this review came from the Database of MEDLINE (period of 1.91.2005), and the keywords of severe acute pancreatitis and pancreatic encephalopathy were used in the searching. RESULTS: Many factors were involved in the pathogenesis of PE in SAP. Pancreatin activation, excessive release of cytokines and oxygen free radicals, microcirculation abnormalities of hemodynamic disturbance, ET-1.NO ratio, hypoxemia, bacterial infection, water and electrolyte imbalance, and vitamin B1.deficiency participated in the development of PE in SAP. CONCLUSIONS: The pathogenesis of PE in SAP has not yet been fully understood. The development of PE in SAP may be a multi-factor process. To find out the possible inducing factor is essential to the clinical management of PE in SAP.

关键词： severe acute pancreatitis pancreatic encephalopathy pathogenesis microcirculation cytokines phospholipase A(2) oxygen free radicals vitamin B1.deficiency

美沙拉嗪肠溶片与柳氮磺吡啶肠溶片治疗溃疡性结肠炎的疗效比较

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中国肛肠病杂志 2024年第1期44卷 40-42页

作者：陈喜翠宋佳韩波安阳市人民医院河南安阳455000

目的:观察比较美沙拉嗪肠溶片与柳氮磺吡啶肠溶片治疗溃疡性结肠炎的疗效。方法:将我院201.年1.至2023.1.诊治的溃疡性结肠炎患者96例随机分为对照组和观察组,每组48例。对照组患者给予口服柳氮磺吡啶肠溶片治疗,观察组患者给予口服... 详细信息

目的:观察比较美沙拉嗪肠溶片与柳氮磺吡啶肠溶片治疗溃疡性结肠炎的疗效。方法:将我院201.年1.至2023.1.诊治的溃疡性结肠炎患者96例随机分为对照组和观察组,每组48例。对照组患者给予口服柳氮磺吡啶肠溶片治疗,观察组患者给予口服美沙拉嗪肠溶片治疗,比较2组患者的临床疗效、炎性因子水平、结肠镜检评分及不良反应发生情况。结果:观察组患者临床总有效率为93.75%,明显高于对照组的62.50%(P0.05);治疗结束时,2组患者IL-8、IL-6、TNF-α水平均低于治疗前(P0.05);治疗结束时,2组患者结肠镜检评分均低于治疗前,且观察组低于对照组(P<0.05)。观察组患者不良反应发生率明显低于对照组(P<0.05)。结论:与柳氮磺吡啶肠溶片相比,美沙拉嗪肠溶片治疗溃疡性结肠炎疗效显著,不但可以降低炎症水平,而且安全性更高。

关键词：溃疡性结肠炎美沙拉嗪肠溶片柳氮磺吡啶肠溶片疗效比较

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数字健康技术在膝骨关节炎运动处方中的应用

中国骨与关节杂志 2024年第1期13卷 57-62页

作者：钱琨于辰曦孙水山东第一医科大学附属省立医院骨关节科济南250021

膝骨关节炎(knee osteoarthritis,KOA)是一种常见的慢性关节疾病,由于过度机械负荷、炎症、代谢和衰老等因素导致关节软骨和软骨下骨损伤、骨赘形成、滑膜增生等病理改变,引起患者出现疼痛、僵硬、关节活动受限和肌肉无力等症状[1.3.。... 详细信息

膝骨关节炎(knee osteoarthritis,KOA)是一种常见的慢性关节疾病,由于过度机械负荷、炎症、代谢和衰老等因素导致关节软骨和软骨下骨损伤、骨赘形成、滑膜增生等病理改变,引起患者出现疼痛、僵硬、关节活动受限和肌肉无力等症状[1.3.。当前全球约有2.5亿人罹患KOA,我国KOA患病率约为8.1.,不同地区因经济发展和地理位置等因素有所差异[4-5]。

关键词：骨关节炎膝运动处方数字健康技术综述