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Effects of adenoviral gene transfer of mutated IκBα,a novel inhibitor of NF-κB,on human monocyte-derived dendritic cells

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Acta Pharmacologica Sinica 2006年第5期27卷 609-616页

作者： Lin-fu ZHOU~(2.5) Ming-shun ZHANG~3.Kai-sheng YIN~(2.5) Yong JI~4 Wei-ping XIE~2.Xue-fan CUI~2.Xiao-hui JI~3.~2.epartment of Respiratory Medicine,the First Affiliated Hospital.~3.epartment of Microbiology and Immunology.~4Atheroscleorosis Research center,nanjing Medical {2.,nanjing 2.002.,china Department of Respiratory Medicine the First Affiliated Hospital Nanjing Medical University Nanjing 210029 China Department of Microbiology and Immunology Nanjing Medical University Nanjing 210029 China Atheroscleorosis Research center Nanjing Medical University Nanjing 210029 China

Aim:To investigate the effects of adenoviral gene transfer of IκBα mutant (IκBαM),a novel inhibitor of nuclear factor κB(NF-κB),on apoptosis,phenotype and func-tion of human monocyte-derived dendritic cells (DC).Methods:Monocytes,cocultured with granulocyte/macrophage colony-stimulating factor (GM-CSF;900ng/mL) and interleukin (IL)-4 (3.0ng/mL) for 5d,followed by stimulation withlipopolysaccharide (LPS;100ng/mL) for 2. differentiated into mature ***-cytes were either left untransfected or were transfected with AdIκBαM or *** transcription and expression of the IκBαM gene,and the inhibitory effect ofIκBαM on tumor necrosis factor (TNF)-α-induced NF-κB activation in mature DCwere detected by polymerase chain reaction (PCR),reverse transcription-poly-merase chain reaction (RT-PCR),Western blot analysis,and electrophoretic mobil-ity shift assays,*** phenotype,apoptosis,IL-12.secretion level ofDC,and ability to stimulate the proliferation of T cells were determined by flowcytometry,enzyme-linked immunosorbent assay and mixed leukocyte ***:PCR and RT-PCR were used to detect a unique 801 bp band in AdIκBαM-transfected mature DC,and also a dose-and time-dependent expression of theIκBαM gene,which peaked at a multiplicity of infection of 100pfu/cell and at ***,AdIκBαM significantly suppressed the TNF-α-induced NF-κBactivation,augmented apoptosis,downregulated CD80,CD83.and CD86 surfacemolecules,IL-12.secretion levels and the ability to stimulate the proliferation of Tcells in mature ***:AdIκBαM effectively transfected and potentlyinhibited NF-κB activation in monocyte-derived mature *** ofthe IκBαM gene in mature DC may contribute to T-cell immunosuppression throughinduction of DC apoptosis and downregulation of B7 molecules,providing a po-tential strategy for future DC-based immunotherapy of asthma.

关键词： dendritic cells nuclear factor κB IκBαmutant immunotherapy asthma

Multivariate analysis in dam monitoring data with PCA

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Science china(Technological Sciences) 2010年第4期53卷 1088-1097页

作者： YU Hong1,2.WU ZhongRu1,2.BAO TengFei1,2.& ZHANG Lan1,2.1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai university,nanjing 2.0098,{2. 2.National Engineering Research {3. of Water Resources Efficient Utilization and Engineering Safety,Hohai university,nanjing 2.0098,{2. 1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China2. National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Hohai University Nanjing 210098 China

Given the limitation of traditional univariate analysis method in processing the multicollinearity of dam monitoring data,this paper reconstructs the multivariate response variables by introducing principal component analysis(PCA) method,explores the ways of determining principal components(PCs),and extracts a few PCs that have major influence on data *** steady observation series,a control field for the whole observation values has been established based upon PCA;for unsteady observation series that have significant tendency,a control field for the future observation values has been constructed according to PC statistical predication *** methods have already been applied to an actual project and the results showed that data interpretation method with PCA can not only realize data reduction,lower data redundancy,and reduce noise and false alarm rate,but also be effective to data analysis,having a broad application prospect.

关键词： dam safety monitoring multivariate response variables principal component analysis data reduction

Assessment model of dam operation risk based on monitoring data

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Science china(Technological Sciences) 2007年第z1期50卷 144-152页

作者： WU ZhongRu1,2., SU HuaiZhi1,2.& GUO HaiQing1 1 College of Water Conservancy and Hydropower Engineering, Hohai university, nanjing 2.0098,{2. 2.National Engineering Research {3. of Water Resources Efficient Utilization and Engineering Safety, nanjing 2.0098,{2. 1. College of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 China 2. National Engineering Research center of Water Resources Efficient Utilization and Engineering Safety Nanjing 210098 China

Although the dams produce remarkable social and economic benefits, the threat made by unsafe dams to the life and property of people who live in the lower river area is un-negligible. Based on the monitoring data which reflect the safety condition of dams, the risk degree concept is proposed and the analysis system and model for evaluating risk degree (rate) are established in this paper by combining the reliability theory and field monitoring data. The analysis method for risk degree is presented based on Bayesian approach. A five-grade risk degree system for dam operation risk and corresponding risk degree is put forward according to the safety condition of dams. The operation risks of four cascade dams on some river are analyzed by the model and approach presented here and the result is adopted by the owner.

关键词： dam monitoring data operation risk degree analysis model

Application analysis of empirical mode decomposition and phase space reconstruction in dam time-varying characteristic

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Science china(Technological Sciences) 2010年第6期53卷 1711-1716页

作者： ZHANG ZhiJun1,2.3.GU ChongShi1,2.BAO TengFei1,2.ZHANG Lan1,2.& YU Hong1,2.1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai university,{2. 2.0098,{2. 2.National Engineering Research {3. of Water Resources Efficient Utilization and Engineering Safety,Hohai university,{2. 2.0098,{2. 3.Department of Civil,Environmental and Geomatic Engineering,Swiss Federal Institute of Technology,Zurich 8093.Switzerland 1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China2. National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Hohai University Nanjing 210098 China3. Department of Civil Environmental and Geomatic Engineering Swiss Federal Institute of Technology Zurich 8093 Switzerland

In view of some courses of the time-varying characteristics processing in the analysis of dam deformation,the paper proposes a new method to analyze the dam time-varying characteristic based on the empirical mode decomposition and phase space reconstruction *** of all,to reduce the influences on the traditional statistical model from human factors and assure the analysis accuracy,response variables of the time-varying characteristic are obtained by the way of the empirical mode decomposition;and then,a phase plane of those variables is reconstructed to investigate their processing *** methods have already been applied to an actual project and the results showed that data interpretation with the assists of empirical mode decomposition and phase space reconstruction is effective in analyzing the perturbations of response variables,explicit in reflecting the entire development process,and valid for obtaining the evolution rules of the time-varying *** methodology is a powerful technical support for people to further master the rules of dam operation.

关键词： dam safety monitoring dam time-varying characteristic empirical mode decomposition phase space reconstruction

Hydrothermal Treatment of Sol-Gel TiO2.Film

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稀有金属材料与工程 2006年第S3期 570-571页

作者： Shaohua Zhang~1 Bing Xie~2.Fengyi Li~3.Tude Zou~2.(1.School of Materials,Nanchang university,Jiangxi 3.0047,china) (2.{2. of {3. and Test,Nanchang university,Jiangxi 3.0047,china) (3.School of Science,Nanchang university,Jiangxi 3.0047,china)

The preparation of TiO2.ilm has been an important research *** our work,we obtained the TiO2.ilm by hydrothermal treating the amorphous film prepared by sol-gel method or the hydrolysis of titanium *** influence of temperature and duration time on the film properties was *** photo degradation of methyl orange in aqueous solution of the film was *** films were characterized by XRD and Uv-Vis spectroscope.

关键词： titania film hydrothermal sol-gel

Solar release time of solar energetic particles and associated acceleration source in corona

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Chinese Science Bulletin 2008年第2期53卷 161-168页

作者： LE GuiMing1,2.3.4, TANG YuHua1 & HAN YanBen4 1 Department of Astronomy, nanjing university, {2. 2.0093.{2. 2.Key Laboratory of Radiometric Calibration and Validation for Environmental Satellites,{2. Meteorological Administration, Beijing 100081,{2. 3.National center for Space Weather,{2. Meteorological Administration, Beijing 100081,{2. 4 National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012.{2. Department of Astronomy Nanjing University Nanjing China Key Laboratory of Radiometric Calibration and Validation for Environmental Satellites China Meteorological Administration Beijing China National center for Space Weather China Meteorological Administration Beijing China National Astronomical Observatories Chinese Academy of Sciences Beijing China

Solar energetic particles (SEPs) are accelerated in corona at an early phase of solar energetic particle events (SEPE). The acceleration mechanism of SEPs in corona can only be inferred from an analysis of multi-band observational data, as the observation of SEPs is usually made around 1AU. In this context, people have investigated spectrums, charge state, solar release time (SRT), and multi-band data of SEPEs, in an attempt to judge the acceleration mechanism of SEPs. The SRT computation of SEPs is an important and commonly used approach to study the acceleration mechanism of SEPs in corona. This paper reviews some important findings concerning the SRT computation of SEPs, and analyzes different merits of each approach for such calculation, based on a range of SEPE case studies. This paper also analyzes and discusses both possible and actual acceleration mechanisms of a number of SEPEs, by calculating the SRT of the SEPEs. Finally, the paper summarizes the possible problems in studying an acceleration mechanism of SEPEs inferred from the SRT of SEP.

关键词：阳光释放时间加速度电晕太阳耀斑

New evidence for lack of CMB power on large scales

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Science china(Physics,Mechanics & Astronomy) 2010年第3期53卷 567-570页

作者： LIU Hao1 & LI TiPei1,2.3.1 Key Laboratory of Particle Astrophysics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, china 2.Department of Physics & {2. for Astrophysics, Tsinghua {2., Beijing 100084, china 3.Department of Engineering Physics & {2. for Astrophysics, Tsinghua {2., Beijing 100084, china 1. Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China2. Department of Physics & Center for Astrophysics Tsinghua University Beijing 100084 China3. Department of Engineering Physics & Center for Astrophysics Tsinghua University Beijing 100084 China

A digitalized temperature map is recovered from the first light sky survey image published by the Planck team, from which an angular power spectrum of the cosmic microwave background (CMB) is derived. The amplitudes of the low multipoles (low-l) measured from the preliminary Planck power spectrum are significantly lower than those reported by the WMAP team. Possible systematical effects are far from enough to explain the observed low-l differences.

关键词： cosmology cosmic microwave background data analysis

Design,analysis and control of hybrid excited doubly salient stator-permanent-magnet motor

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Science china(Technological Sciences) 2010年第1期53卷 188-199页

作者： ZHU XiaoYong1,2.& CHENG Ming1 1 Engineering Research center for Motion Control of MOE,School of Electrical Engineering,Southeast university,{2. 2.0096,{3. 2.School of Electrical and Information Engineering,Jiangsu university,Zhenjiang 2.2.13.{3. 1. Engineering Research center for Motion Control of MOE School of Electrical Engineering Southeast University Nanjing 210096 China2. School of Electrical and Information Engineering Jiangsu University Zhenjiang 212013 China

In this paper,a new hybrid excited doubly salient stator-permanent-magnet motor is proposed,where both permanent magnets and DC field windings are located in the *** theory,the mathematical model and design method of the motor are *** corresponding output power equation and the relationship between the flux control capability and the extended speed range are deduced in *** using an efficient "one-step" three-dimensional(3.) finite element method,the electromagnetic performances are analyzed,in which the leakage flux outside the stator circumference and end-effect are taken into *** on the operation principle of the motor,the control strategy and scheme are developed and implemented *** experimental results and finite element analysis show that the proposed motor not only maintains the advantages of doubly salient permanent magnet motors,but also offers high energy efficiency over a wide speed range,which makes the motor an interesting candidate for electric vehicles.

关键词： stator permanent magnet motor hybrid excitation doubly salient finite element method flux weakening control

Phylogeographic analysis of human papillomavirus 58

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Science china(Life Sciences) 2009年第12期52卷 1164-1172页

作者： LI YanYun1, LI ZuoFeng2. HE YiFeng1, KANG Yu1, ZHANG XiaoYan1, CHENG MingJun1, ZHONG Yang2. 3. & XU CongJian1· 1 Obstetrics and Gynecology Hospital of Fudan university, Shanghai 2.0011,{2. 2.Shanghai Bioinformation Research {2., Shanghai 2.02.0},{2. 3.School of Life Sciences, Fudan university, Shanghai 2.043.,{2. Obstetrics and Gynecology Hospital of Fudan university Shanghai 200011 China Shanghai Bio-information Research center Shanghai 200235 China School of Life Sciences Fudan University Shanghai 200433 China

Human papillomavirus 58 (HPV58) is one type of HPV with high risk of causing cervical cancer. Unusually high prevalence of HPV58 has been reported in Asia, Africa and some other areas. However, due to the scattered distribution of global data, in addition to the lack of data of some HPV58 high-incidence nations and regions, like china's Mainland, a comprehensive analysis of the global geographical distribution of HPV58 remains blank so far. In this study, HPV58 from the human cervical cancer tissue was detected in china's Mainland, and 14 new HPV58-E6/L1 gene sequences were obtained. Moreover, phylogeographic analysis has been conducted combining the HPV58 sequences that have been deposited in GenBank since 1985. The study result shows that the sequences detected from the Shanghai, Jiangsu and Sichuan areas are homologous with those found in the past from Hong Kong and Xi'an, china, as well as Japan and other Southeast Asian areas. Furthermore, Western Africa is considered to be the "root" source of the HPV58 variant, while china's Mainland and Southeast Asia are "transit points" and the new sources of HPV58 after receiving the isolates from the "root" source; like HPV16 and HPV18, the HPV58 might also be one of the major HPV types associated with the development and spread of cervical cancer.

关键词： human papillomavirus 58 (HPV58) cervical cancer phylogeography molecular evolution