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TRPC3.is involved in flow- and bradykinin-induced vasodilation in rat small mesenteric arteries

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Acta Pharmacologica Sinica 2006年第8期27卷 981-990页

作者： Cui-ling LIU~(2,4) Yu HUANG~3.Ching-yuen NGAI~3.Yuk-ki LEUNG~3.Xiao-qiang YAO~3.~2Department of Pathology,peking university Health Science Center,beijing 100083.china ~3.epartment of Physiology Faculty of Medicine,the Chinese {3. of Hong Kong,Hong Kong,china Department of Pathology Peking University Health Science Center Beijing China Department of Physiology Faculty of Medicine the Chinese University of Hong Kong Hong Kong China

Aim:To test the possible involvement of TRPC3.in agonist-induced relaxationand flow-induced vasodilation in rat small mesenteric ***:MaleSprague-Dawley rats were used in the present *** 72 h-treatment ofantisense oligo via tail vein injection,isometric tension and isobaric diametermeasurement were carried out with isolated mesenteric artery segments by usingeither a Pressure Myograph or a Multi Myograph *** [Ca2+]ichanges were measured with a MetaFluor imaging system in response to flow orto 3. nmol/L ***:Immunohistochemical study showed that the72 h-treatment of antisense oligo via tail vein injection markedly decreased theTRPC3.expression in mesenteric arteries,indicating the effectiveness of theantisense *** tension and isobaric diameter measurement showedthat,although the antisense oligo treatment did not affect histamine-,ATP-,andCPA-induced relaxation,it did reduce the magnitude of flow-induced vasodilationby approximately 13. and decreased bradykinin-induced vascular relaxation withits EC50value raised by nearly *** [Ca2+]imeasurement revealedthat treatment of the arteries with antisense oligos significantly attenuated themagnitude of endothelial [Ca2+]irise in response to flow and to 3. nmol/L ***:The results suggest that TRPC3.is involved in flow-and bradykinin-induced vasodilation in rat small mesenteric arteries probably by mediating theCa2+influx into endothelial cells.

关键词： TRPC3 flow-induced vasodilation bradykinin-induced vasodilation antisense oligonucleotides

Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA

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Science china Chemistry 2006年第1期49卷 75-80页

作者： ZHANG Huijuan1,2, WANG Jin3. WU Yishi1, YUAN Gu3. AI Xicheng1, WANG Li4 & ZHANG Jianping1 1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, beijing 100080, china 2. School of Life Science and Technology, beijing Institute of Technology, beijing 100081, china 3. College of Chemistry and Molecular Engineering, peking university, beijing 100080, china 4. Center for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, beijing 100080, china Correspondence should be addressed to Ai Xicheng and Zhang Jianping State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing China School of Life Science and Technology Beijing Institute of Technology Beijing China College of Chemistry and Molecular Engineering Peking University Beijing China Center for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing China

The photophysical properties of the polyamide PyPyPyβDp (PPP) were investigated by means of steady-state absorption and fluorescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. It was found that the excited-state properties of PPP are very sensitive to solvents. In TKMC buffer PPP exhibited weak fluorescence with a decay time constant of 16 ps, while with the decrease of the solvent polarity PPP showed the blue-shifted peak position, increased inten-sity and lengthened life-time for its fluorescence behavior. In the presence of calf thymus DNA, it was observed that the fluorescence intensity was enhanced and the fluorescence lifetime increased from 16 to 3. ps for PPP, which verified that PPP bound into the minor groove of DNA duplex.

关键词： polyamide (PPP), calf thymus DNA (ctDNA), time-resolved fluorescence spectroscopy, recognition dynamics.

Taurine inhibits ischemia/reperfusion-induced compartment syndrome in rabbits

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Acta Pharmacologica Sinica 2005年第7期26卷 821-827页

作者： Ji-xian WANG~(2,3. Yan LI~2 Li-ke ZHANG~4 Jing ZHAO~5 Yong-zheng PANG~(2,3. Chao-shu TANG~(3.5) Jing ZHANG~(2,3.6) 1 Institute of Cardiovascular Disease Research,The First Hospital of peking university,beijing 10003.,china 3.Key Laboratory of Molecular Cardiovascular Science,Ministry of Education,beijing 100083.china 4 Department of Pathophysiology,Capital Institute of Medicine,beijing 100054,china 5 Department of Physiology Health Center of Medical Sciences,peking university,beijing 100083.china Institute of Cardiovascular Disease Research The First Hospital of Peking University Beijing China Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing China Department of Pathophysiology Capital Institute of Medicine Beijing China Department of Physiology Health Center of Medical Sciences Peking University Beijing China

Aim:To investigate effects of taurine on ischemia/reperfusion(I/R)-induced com-partment syndrome in rabbit hind ***:Rabbits underwent femoralartery occlusion after ligation of branches from terminal aorta to femoral *** a 7-h ischemia,reperfusion was established with the use of heparinizedpolyethylene *** received taurine(1 g/kg)or normal saline(control)by iv infusion 10 min before shunt *** reperfusion,anterior com-partment pressure(ACP)was monitored continuously in the left lower *** muscle triphenyltetrazolium chloride(TTC)level,taurine contentand myeloperoxidase activity were *** stress was induced in thein vitro gastrocnemius muscle slices by free radical generating systems(FRGS),and the malondialdehyde content was measured in presence or absence of ***:After 7 h of ischemia,none of the parameters that we measured weredifferent from those before ischemia,except that TTC reduction decreased by80%.In the control group,after 2 h of reperfusion,ACP increased 4.5-fold,andgastrocnemius muscle taurine content was reduced by 3.%.In taurine-treatedanimals,at 2 h reperfusion,the mean arterial blood pressure and heart rate wereincreased,by 6% and 10%.ACP decreased by 3.%,muscle edema decreased by16%,TTC reduction increased by 150%,and lactate dehydrogenase decreased by3.% compared to control *** and muscle taurine content increased by70% and 88%,*** the taurine-treated group,at 2 h repeffusion,plasmamalondialdehyde and conjugated diene content were decreased by 3.% and 23.,respectively,and muscle malondialdehyde and conjugated diene content decreasedby 22% and 3.%,respectively compared to the control *** 2 h reperfusion,myeloperoxidase activity was increased 3.5-fold in control *** the in vitrostudy,taurine decreased malondialdehyde content in muscle slices incubatedwith hypochlorous acid in a dose-dependent manner,but there was no changewhen incubated

关键词： taurine compartment syndromes reperfu sion injury

Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells

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Acta Pharmacologica Sinica 2004年第3期25卷 87-92页

作者： Lin CHANG2,3.Jian-xin XU4,Jing ZHAO5,Yong-zheng PANG2,3.Chao-shu TANG2,3.Yong-fen QI5,6 2Institute of Cardiovascular Diseases,the FirstHospital,peking university,beijing 10003. 3.nstitute of Cardiovascular Diseases,peking university,beijing 100083.4Instituteof Biophysics,Chinese Academy of Sciences,beijing 100101 5Department of Physiology,Health Science Center,peking university,beijing 100083.china Institute of Cardiovascular Diseases the First Hospital Peking University 北京 100034 Institute of Biophysics Chinese Academy of Sciences 北京 100101 Department of Physiology Health Science Center Peking University 北京 100083

AIM: To observe protective effects of taurine on reactive oxygen species generation induced by homocysteine in rat vascular smooth muscle cells (VSMC). METHODS: Rat VSMC was incubated with various concentrations of homocysteine and taurine. The lactate dehydrogenase (LDH) activity which released into culture medium was elevated as an indicator for VSMC injury. The reactive oxygen species (ROS) — hydrogen peroxide (H2O2) and superoxide anion (O2ˉ· )were measured with luminol or lucigenin chemiluminescences method, and the mitochondria Mn-superoxide dismutase (Mn-SOD) and catalase (CAT) were also measured in treated VSMC. RESULTS: LDH leakage from cultured VSMC treated with homocystenie, was increased (P0.05 vs control). In this study, the superoxide anion in VSMC was not detectable by chemiluminent method. In addition, treatment of VSMC with taurine increased mitochondria Mn-SOD and CAT activity in a concentration dependent manner (P<0.05), but homocysteine decreased mitochondria Mn-SOD and CAT activity (P<0.01 vs control). In addition, co-administration of taurine markedly ameliorated homocysteine-induced inhibition of Mn-SOD and CAT activity in VSMC (P<0.01 vs homocysteine alone group). CONCLUSION: Taurine antagonized the effects of homocysteine on ROS generation and anti-oxidant enzyme activities in rat VSMC in vitro.

关键词： homocysteine taurine vascular smooth muscle mitochondria reactive oxygen species

Applications of functionalized ionic liquids

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Science china Chemistry 2006年第5期49卷 385-401页

作者： LI Xuehui1, ZHAO Dongbin2,3. FEI Zhaofu2 & WANG Lefu1 1. Department of Chemical Engineering, Guangdong Provincial Laboratory of Green Chemical Technology, South china Uni-versity of Technology, Guangzhou 510641, china 2. Swiss Federal Institute of Technology, Lausanne, EPFL, CH-1015 Lausanne, Switzerland 3. PKU Green Chemistry Center, beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, {3., beijing 100871, china Department of Chemical Engineering Guangdong Provincial Laboratory of Green Chemical Technology South China University of Technology Guangzhou China Lausanne EPFL Swiss Federal Institute of Technology Lausanne Switzerland PKU Green Chemistry Center Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing China

Recent developments of the synthesis and applications of functionalized ionic liquids (including dual-functionalized ionic liquids) have been highlighted in this review. Ionic liquids are at-tracting attention as alternative solvents in green chemistry, but as more functionalized ILs are pre-pared, a greater number of applications in increasingly diverse fields are found.

关键词： ionic liquids functionalized ionic liquids dual-functionallzed ionicliquids reaction media asymmetric synthesis nano-materials porous materials lubricants flue-gas desulfurization oil desul-furization

Bionic Design and Finite Element Analysis of Elbow in Ice Transportation Cooling System

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Journal of Bionic Engineering 2010年第3期7卷 301-306页

作者： Dejun Miao~(1,2), Xiuhua Sui~3. Linjing Xiao~3.1. Key Laboratory of Mine Hazard Prevention and Control (Ministry of Education, china), Shandong university of Science and Technology, Qingdao 266510, P. R. china 2. School of Architecture, Tsinghua university, beijing 100084, P. R. china 3. College of Mechanical and Electronic Engineering, Shandong university of Science and Technology, Qingdao 266510, P. R. china Key Laboratory of Mine Hazard Prevention and Control (Ministry of Education China) Shandong University of Science and Technology Qingdao P. R. China School of Architecture Tsinghua University Beijing P. R. China College of Mechanical and Electronic Engineering Shandong University of Science and Technology Qingdao P. R. China

With the increase in mining depth, mine heat harm has appeared to be more prominent. The mine heat harm could be resolvedor reduced by ice refrigeration. Thus, ice transportation through pipeline becomes a critical problem; typically flowresistance occurs in the elbow. In the present study, according to the analysis of the surface morphology of fish scale, abiomimetic functional surface structure for the interior wall of elbow is designed. Based on the theory of liquid-solid two phaseflow, a CFD numerical simulation of ice-water mixture flowing through the elbow is carried out using finite element *** experiments of pressure drop and flow resistance for both bionic and common elbows are conducted to test theeffect of the bionic elbow on flow resistance reduction. It is found that with the increase in the ice mass fraction in the ice-watermixture, the effect of bionic elbow on resistance reduction becomes more obvious.

关键词： bionic elbow two phase flow ice transportation flow resistance reduction pressure loss

Lithosphere types in North china:Evidence from geology and geophysics

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Science china Earth Sciences 2005年第11期48卷 1809-1827页

作者： QIU Ruizhao1,2, DENG Jinfu3. ZHOU Su3. LI Jinfa4, XIAO Qinghui1,5, WU Zongxu6 & LIU Cui3.1. Institute of Geology, Chinese Academy of Geological Sciences, beijing 10003., china 2. Development and Research Center, china Geological Survey, beijing 10003., china 3. china university of Geosciences, beijing 100083. china 4. china university of Geosciences, Wuhan 43.074, china 5. Information Center of Ministry of Land and Resources, beijing 100812, china 6. Institute of Geology, State Seismological Bureau, beijing 100029, china Institute of Geology Chinese Academy of Geological Sciences Beijing China Development and Research Center China Geological Survey Beijing China china university of Geosciences Beijing China china university of Geosciences Wuhan China Information Center of Ministry of Land and Resources Beijing China Institute of Geology State Seismological Bureau Beijing China

On the basis of the characteristics of geology and geophysics in North china, three types of lithosphere, namely, the cratonic, the orogenic and the rift lithospheres can be classified. In terms of petrological method (based on the information from Precambrian rock assemblages, igneous activities, deep-seated enclaves, etc.) and the relationship between seismic velocity and rock compositions, the crust-mantle petrological and chemical structure models can be set up. Researching results indicate that the geology and geophysics of North china platform bears the similar characteristics in comparison with those of the global typical cratons. The Eerduosi(Ordos) block located in the west of the North china Platform is a remnant of cratonic lithosphere after the North china platform had undergone “activation” in Mesozoic and “reconstruction” in Cenozoic times. The continental crust consists mainly of TTG rock assemblage while the subcontinental lithosphere mantle mainly consists of strongly depleted harzburgite. The craton was finally formed in late Archaean and early Proterozoic, and has been kept in stability up to present; its crustal-mantle petrological structures of lithosphere can be set up as a reference for the study of North china craton and even Sino-Korean craton. In the Mesozoic period, the middle and east areas of North china platform were activated in the Yanshanian orogenic process, the continental crust was reformed by material and heat-transfer of convective mantle and the original crustal TTG component was reconstructed to be granitic crust, and the subcontinental lithosphere man- tle was replaced by the Yanshanian harzburgite-lherzolite. The Yanshan-Taihang Mountains were the remnants of orogenic lithosphere after the rifting in eastern North china in Cenozoic. The present thickness of continental crust and lithosphere in the Yanshan-Taihang Mountains is not equal to their thickness during the Yanshanian orogenic movement because they had undergone the

关键词： lithospheric types,evidence of geology and geophysics,crust-mantle petrological structure,deep process

Distinct actions of intermittent and sustained β-adrenoceptor stimulation on cardiac remodeling

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Science china(Life Sciences) 2011年第6期54卷 493-501页

作者： MA XiaoWei1,3.SONG Yao1,3.CHEN Chao1,3.FU YongNan1,3.SHEN Qiang2,3.LI ZiJian1,3.& ZHANG YouYi1,3.1Institute of Vascular Medicine,peking university Third Hospital,beijing 100191,china 2Institute of Cardiovascular Science,peking university,beijing 100191,china 3.ey Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,beijing 100191,china Institute of Vascular Medicine Peking University Third Hospital Beijing China Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China Institute of Cardiovascular Science Peking University Beijing China

Heart disease is associated with increased sympathetic nerve activity and elevated levels of circulating catecholamines,resulting in chronic stimulation of the β-adrenergic receptors (β-AR) and consequent pathological cardiac ***,chronic administration of the β-AR agonist isoproterenol (ISO) has been most commonly used to model β-AR-induced cardiac ***,it remains unclear whether β-AR-mediated cardiac remodeling and dysfunction differs between sustained versus pulsatile (intermittent) exposure to a β-***,we compare the effects of intermittent versus sustained administration of ISO on cardiac remodeling and function in *** were administered 5 mg (kg d)-1 ISO for 2 weeks either by daily subcutaneous injection,or continuous infusion via an implanted osmotic *** function and remodeling were determined by echocardiography,micromanometry and ***,Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to define the proteins and genes *** sustained and intermittent administration of ISO resulted in a similar degree of cardiac hypertrophy (16% and 19%,respectively).However,mice receiving ISO by daily injection developed more severe ventricular systolic and diastolic dysfunction and myocardial fibrosis compared with mice receiving ISO via the osmotic *** disparity in results between the delivery methods is suggested to be due,at least in part,to increased expression of fibrogenic factors,including connective tissue growth factor (CTGF) and NADPH oxidase (NOX4),in mice receiving intermittent application of *** summary,compared with sustained exposure to a β-AR agonist,intermittent β-AR stimulation leads to more severe cardiac dysfunction and *** findings not only further our understanding of β-AR function in the setting of cardiac pathophysiology,but also highlight that significant differences can result dependent upon the mod

关键词： β-adrenoceptor reactive oxygen species NADPH oxidase cardiac hypertrophy cardiac fibrosis

Southeastern extension of the Red River fault zone (RRFZ) and its tectonic evolution significance in western South china Sea

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Science china Earth Sciences 2006年第8期49卷 839-850页

作者： LIU Baoming1,2,3. XIA Bin1, LI Xuxuan1,3. ZHANG Minqiang1,3. NIU Binhua2, ZHONG Lifeng1, JIN Qinghuan1,4 & JI Shaocheng1,5 1. Key Laboratory of Marginal Sea Geology and Resources, Chinese Academy of Sciences,Guangzhou 510640, china 2. china university of Geosciences ({3.), {3. 100083. china 3. Research Center of Science & Technology Department, CNOOC, {3. 100027, china 4. Guangzhou Marine Geological Survey, Ministry of Land & Resources, Guangzhou 510075, china 5. Ecole Polytechnique Montreal, H3.3.7, Canada Key Laboratory of Marginal Sea Geology and Resources Chinese Academy of Sciences Guangzhou China china university of Geosciences (beijing) Beijing China Research Center of Science & Technology Department CNOOC Beijing China Guangzhou Marine Geological Survey Ministry of Land & Resources Guangzhou China Ecole Polytechnique Montreal Canada

Recent geophysical surveys and basin modeling suggest that the No.1 fault in the Ying- gehai basin (YGHB) is the seaward elongation of the Red River fault zone (RRFZ) in the South china Sea (SCS). The RRFZ, which separates the South china and Indochina block, extends first along the Yuedong fault, offshore of Vietnam, and then continues southward and breaks off into two branches: the Lupar fault and the Tinjia fault. The southern extension of the Lupar fault dies out beneath the NW Borneo while the Tinjia fault extends southeast and reaches the Brunei-Sabah area. According to the gravity and geomagnetic data, and the tectonic evolution of the basins, there are different evolution histories between the Wan’an basin (WAB) and the basins in the Nansha block. The Tinjia fault may be the boundary between the Balingian block and the Nansha block. Hence, the line linking the Yue- dong fault and the Tinjia fault, which both are continental margin faults and strike-slip ones in the geological evolution histories, constitute the boundary between the Indochina and Nansha block. The Lupar fault, in contrast, is an intraplate fault within the Indochina block. The results provide new hints for reconstructing the tectonic evolution history of the RRFZ and the opening of the SCS, and also a framework for hydrocarbon prospecting in the region.

关键词： Red River fault zone, tectonic evolution, fault, extension, western South china Sea.