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Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin WANG~1 Ting WU~2 Jian-Qiang HUANG~1 Jui-Shen CHIAO~1 Guo-Ping ZHAO~(1,3.4) 1 Laboratory of Molecular Microbiology,institute of plant physiology and {3.,shanghai institutes for biological Sciences,chinese academy of Sciences,shanghai 20003.,china 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 20003.,china 3.State Key Laboratory of Genetic Engineering/Department of Microbiology,School of Life Science,Fudan University,shanghai 20043.,china 4 Laboratory of Health and Disease Genomics,chinese National Haman Genome Center at shanghai,shanghai 201203.china

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U3. wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of466 amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72% identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U3. led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U3.,the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

Proteomic analysis of PM proteins involved in rice defense responses

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Proteomic analysis of PM proteins involved in rice defense r...

第二届全国植物蛋白质组学学术研讨会

作者： Fang Chen Zuhua He shanghai institute of plant {3. and ecology,shanghai institutes of biological Sciences,chinese academy of Sciences,shanghai,china

PM is the first cell component to sense environmental *** establishment of a cellular model for bacterial blight (Xanthomonas oryzae ***, Xoo) infection and method improvement of PM protein preparation,we successfully...

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Characterization of replication and conjugation of plasmid p WTY27 from a widely distributed Streptomyces species

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Characterization of replication and conjugation of plasmid p...

2012年第五届全国微生物遗传学学术研讨会

作者： Tao Wang shanghai institute of plant {3. and ecology,shanghai institutes for biological Sciences, chinese academy of Sciences

We report the detection of a widely distributed Streptomyces strain Y27 and its indige nous plasmid pWTY27 from plants and soil samples cross china by both culturing an d nonculturing *** complete nucleotide sequence of pWTY27 consisted of 14,288 bp.A basic locus for plasmid replication comprised repAB genes and an adjac ent iteron sequence,to a long inverted-repeat of which the RepA protein bound specifi cally in vitro,suggesting that RepA may recognize a second structure(e.g.a long ste m-loop) of the iteron DNA.A plasmid containing the locus propagated in linear mode when the telomeres of a linear plasmid were attached,indicating a bi-directional repli cation mode for *** for rolling-circle plasmids,a single traA gene and a tns sequence(covering 16 bp within traA and its adjacent 159 bp) on pWTY27 were requ ired for plasmid *** recognized and bound specifically to the two regions o f the tns sequence,one containing all the four DR1 of 7 bp(TGACACC) and one DR 2(CCCGCCC) and most of IR1,and another covering two DR2 and part of IR 1,suggesting formation of a high-ordered DNA-protein complex.

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Preliminary studies on differential defense responses induced during plant communication

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Cell Research 2005年第3期15卷 187-192页

作者： Jin Ying PENG1,3.Zhong Hai LI2,Hui XIANG1,3.Jian Hua HUANG1,3.Shi Hai JIA1,3.Xue Xia MIAO1,Yong Ping HUANG1* 1institute of plant physiology and {3.,shanghai institute for biological Sciences,Graduate School of the chinese academy of Sciences,chinese academy of Sciences,3.0 Fenglin Road,shanghai 20003.,{13. 2chinese National Human Genome Center at shanghai,250 Bibo Road,shanghai 201203.{13. 3.raduate School of the chinese academy of Sciences,Beijing 10003.,{13. instituteofplantphysiologyand{3. ShanghaiInstituteforBiologicalSciencesGraduateSchooloftheChineseAcademyofSciencesChineseAcademyofSciences300FenglinRoadShanghai200032China

We compared the expression patterns of three representative genes in undamaged tomato and tobacco plants in response to exposure to either tomato or tobacco fed on by Helicoverpa armigera (cotton bollworm). When tomato and tobacco, two species of one family, were incubated in the chambers with the tomato plants damaged by the cotton bollworm, the expression of the PR1, BGL2, and PAL genes was up-regulated in leaves of both plants. However, the levels of gene expression were significantly higher in the tomato than that in the tobacco. In addition, the activities of enzymes, peroxidase, polyphenol oxidase, and lipoxygenase were found to be higher in the tomato than those in the tobacco. Similar results were obtained when the damaged plants were replaced by the tobacco.

关键词： plant-plant communication tomato (Lycopersicon esculentum) tobacco (Nicotiana tabacum) cotton boll- worm (Helicoverpa armigera) inducible defenses.

Sequencing and Analysis of a Genomic Fragment Provide an Insight into the Dunaliella viridis Genomic Sequence

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 812-820页

作者： Xiao-Ming SUN~(1#) Yuan-Ping TANG~(1#) Xiang-Zong MENG~1 Wen-Wen ZHANG~1 Shan LI~1 Zhi-Rui DENG~1 Zheng-Kai XU~(1,2) Ren-Tao SONG~1* 1 shanghai Key Laboratory of Bio-energy Crops,School of Life Sciences,shanghai University,shanghai 200444,{3. 2 institute of plant physiology & ecology,shanghai institutes for biological Sciences,chinese academy of Sciences,shanghai 20003.,{3. shanghaiKeyLaboratoryofBio-energyCrops SchoolofLifeSciencesShanghaiUniversityShanghai200444China instituteofplantphysiology&{3. ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200032

Dunaliella is a genus of wall-less unicellular eukaryotic green *** exceptional resistancesto salt and various other stresses have made it an ideal model for stress tolerance ***,very littleis known about its genome and genomic *** this study,we sequenced and analyzed a 29,268 bpgenomic fragment from DunalieIla *** fragment showed low sequence homology to the *** the nucleotide level,only a segment with significant sequence homology to 18S rRNA *** fragment contained six putative genes,but only one gene showed significant homology at theprotein level to GenBank *** average GC content of this sequence was 51.1%,which was muchlower than that of close related green algae Chlamydomonas （65.7%）.Significant segmental duplicationswere found within this *** duplicated sequences accounted for about 3..7% of the *** amounts of simple sequence repeats （microsatellites） were found,with strong bias towards（AC）_n type （76%）.Analysis of other Dunaliella genomic sequences in the GenBank database （total 25,749bp） was in agreement with these *** sequence features made it difficult to sequence Dunaliellagenomic *** investigation should be made to reveal the biological significance of these uniquesequence features.

关键词： Dunaliella viridis genomic sequence sequence feature sequence duplication simple sequence repeat

一种黑豆蚜储存蛋白质的分离纯化及其亚基的性质

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生物化学与生物物理学报 2003年第2期35卷 189-192页

作者：甘明丁德诚周元聪中国科学院上海生命科学研究院植物生理生态研究所中国科学院上海生命科学研究院生物化学与细胞生物学研究所蛋白质组学重点实验室上海20003. 上海20003.

从黑豆蚜 (AphiscraccivoraKoch)血淋巴中经DE5 2纤维素离子交换层析、SephadexG 15 0凝胶过滤以及在FPLC上QSepharose分离出一种蛋白质。该蛋白质经SDS PAGE测定在非还原和还原条件下分子量均为 6 0kD左右 ,等电聚焦电泳测得其等电点约... 详细信息

从黑豆蚜 (AphiscraccivoraKoch)血淋巴中经DE5 2纤维素离子交换层析、SephadexG 15 0凝胶过滤以及在FPLC上QSepharose分离出一种蛋白质。该蛋白质经SDS PAGE测定在非还原和还原条件下分子量均为 6 0kD左右 ,等电聚焦电泳测得其等电点约为 5 .0 ,为糖蛋白。若蚜期间该蛋白质在蚜虫体内积累 ,羽化至成蚜时含量明显减少 ,为蚜虫提供发育所需氨基酸 ,在成蚜期间仍以低浓度存在。根据其分子量和氨基酸组成与含量变化动态应属不完全变态昆虫的一种持续性储存蛋白质。

关键词：黑豆蚜储存蛋白质分离纯化

Molecular Evolution and Regulation of a Broad-Spectrum Disease Resistance Locus in Rice

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Molecular Evolution and Regulation of a Broad-Spectrum Disea...

第十二届全国植物基因组学大会

作者： Yiwen Deng,Zuhua He institute of plant physiology and {3.,shanghai institutes for biological Sciences,chinese academy of Sciences,shanghai 20003.,china

Rice blast(Magnaporthe oryzae) is the most destructive disease of rice in *** breeding for broad-spectrum blast resistance has been the most effective and economical approach to control the disease. The Pigm locus has been identified to confer broad-spectrum and durable resistance to rice *** sequencing revealed that the Pigm locus contains an NB-LRR resistance protein cluster that has undergone duplication,unequal cross and recombination during evolution or *** analysis

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Role of oxyR from Sinorhizobium meliloti in Regulating the Expression of Catalases

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 421-428页

作者： Li EUO~(1.3. Ming-Sheng QI~(1.3. Shi-Yi YAO~2 Hai-Ping CHENG~2 Jia-Bi ZHU~1 Guan-Qiao YU~1 1 National Laboratory of plant Molecular Genetics, institute of plant {3. and ecology, shanghai institutes for biological Sciences.chinese academy of Sciences, shanghai 20003.. china 2 biological Sciences Department, Lehman College, the City University of New York, Bronx, NY 10468. USA, 3.Graduate School of the chinese academy of Sciences, shanghai 20003.. china NationalLaboratoryofplantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologi

The process of symbiotic nitrogen fixation results in the generation of reactive oxygen speciessuch as the superoxide anion（O2-）and hydrogen peroxide（H2O2）.The response of rhizobia to these toxicoxygen species is an important factor in nodulation and nitrogen *** Sinorhizobium meliloti,oneoxyR homologue and three catalase genes,katA,katB,and katC were detected by sequence *** gene is located next to and divergently from katA on the *** investigate the possible rolesof oxyR in regulating the expression of catalases at the transcriptional level in ***,an insertion mutantof this gene was *** mutant was more sensitive and less adaptive to H2O2than the wild typestrain,and total catalase/peroxidase activity was reduced approximately fourfold with the OxyR mutationrelative to *** activities of KatA and KatB and the expression of katA::lacZ and katB::lacZ promoterfusions were increased in the mutant strain compared with the parental strain grown in the absence of H2O2,indicating that katA and katB are repressed by ***,when exposed to H2O2,katA expressionwas also increased in both *** and Escherichia *** exposed to H2O2,OxyR is convertedfrom a reduced to an oxidized form in *** concluded that the reduced form of OxyR functions as arepressor of katA and katB ***,in the presence of H2O2,reduced OxyR is converted to theoxidized form of OxyR that then results in increased katA *** further showed that oxyR expressionis autoregulated via negative feedback.

关键词： Sinorhizobium meliloti OxyR catalase katA H2O2

Biosynthesis and Metabolism of Nitric Oxide in Higher plants

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Biosynthesis and Metabolism of Nitric Oxide in Higher Plants

2006年拟南芥研究学术研讨会

作者： Fang-Qing Guo institute of plant physiology & {3., shanghai institutes for biological Sciences, chinese academy of Sciences

＜正＞Nitric oxide （NO） serves as a signal involving in a variety of physiological processes including disease resistance, abiotic stress, cell death, respiration, senescence, root development, germination and hormone responses in higher plants. In mammals, three nitric oxide synthase （NOS） isoforms have been well characterized and are responsible for NO production in various tissues. Following the release of the Arabidopsis genome, not a single gene or protein with sequence similarity to the mammalian NOSs could

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Studies on a PPR protein YELLOW SEEDLING 1 in regulation of chloroplast development at the early growth stage in Arabidopsis

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Studies on a PPR protein YELLOW SEEDLING 1 in regulation of ...

纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Wenbin Zhou and Jirong Huang shanghai institute of plant {3. and ecology, shanghai institutes for biological Sciences, chinese academy of Sciences, shanghai 20003.

＜正＞Plastid transcription is performed by nuclear-encoded RNA polymerases （NEP） and the plastid-encoded RNA polymerase （PEP）. PEP-dependent gene expression is crucial for chloroplast development and is temporall... 详细信息

关键词： Arabidopsis PPR protein regulation of chloroplast development