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Isolation and functional analysis of a Brassica junea gene encoding a component of auxin efflux carrier

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Cell Research 2002年第Z1期 235-245页

作者： WEI MIN NI1,2. XIAO YA CHEN1, ZHI HONG XU1,2. HONG WEI XUE1.2.*1 National Laboratory of plant Molecular Genetics, institute of plant {3. and ecology, shanghai institutes for biological sciences, chinese academy of sciences2.Partner Group of Max-Pla

Polar auxin transport plays a divergent role in plant growth and developmental processes including root and embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsis pin gene family was believed to encode a component of auxin efflux carrier (G(?)lweiler et al, 1998). Based on the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpin1), which encoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level with AtPINl and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homology to AtPIN3.. Hydrophobic analysis showed similar structures between BjPINl and AtPIN proteins. Presence of 6 exons (varying in size between 65 bp and 12.9 bp) and 5 introns (sizes between 89 bp and 463.bp) in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blot analysis indicated that Bjpin1 was expressed in most of the tissues tested, with a relatively h

关键词： efflux carrier Brassica juncea polar auxin transport.

Studies on the Rice Seed Development

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Studies on the Rice Seed Development

第十二届全国植物基因组学大会

作者： Hongwei Xue National Key Laboratory of plant Molecular Genetics,institute of plant {3. and ecology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai {13.China

The developments of rice seeds are closely associated with assimilates storage and plant *** characterize the regulatory networks during rice seed development,transcriptomes of developing embryo and endosperm were *** results showed that genes predominantly expressed in rice seed are mostly involved in metabolism regulation,hormone response and cellular organization ***,many transcription factors are highly expressed in rice seed and/or regulated during seed development,which was further analyzed by constructing the gene co-regulatory *** addition,study of the effects of low tern-

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Molecular mechanism of G-protein-regulated chloroplast development

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Molecular mechanism of G-protein-regulated chloroplast devel...

植物分子生物学与现代农业——2.10全国植物生物学研讨会

作者： Jirong Huang National Key Laboratory of plant Molecular Genetics,institute of plant {3. and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 2.0032.China

Heterotrimeric G-proteins have been demonstrated to play an important role in many physiological processes in *** first evidence for the involvement of G-proteins in chloroplast development came from pharmaceutical studies on tomato hypocotyl cells of the aurea mutant that is defective in phytochrome A(Neuhaus et al.,1993..It

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Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules

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Cell Research 2006年第10期16卷 818-829页

作者： Zi Ying Gong~1 Zhi Shui He~1 Jia Bi Zhu~1 Guan Qiao Yu~1 Hua Song Zou~1 ~1National Laboratory of plant Molecular Genetics,shanghai {2. of plant physiology and ecology,shanghai institutes.for biological sciences,chinese academy of sciences,shanghai 2.0032.China NationalLaboratoryofplantMolecularGenetics ShanghaiInstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200032China

Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in *** understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa,the cDNA-AFLP technique wasemployed to study the changes in gene expression in nifA mutant *** the approximately 3.000 transcript-derived fragments,3. had differential expression *** expression levels were subsequently confirmed by reverseNorthern blot and RT-polymerase chain *** analyses revealed that 2. cDNA fragments corresponded togenes involved in signal communication,protein degradation,nutrient metabolism,cell growth and development.

关键词： cDNA-AFLP gene expression nodule Sinorhizobium meliloti nifA

Cloning of the Gene Responsible for the Yellow Cotyledon and Reduced Accumulation of Anthocyanins in Arabidopsis

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Cloning of the Gene Responsible for the Yellow Cotyledon and...

纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Yi Sun, Jirong Huang shanghai institute of {2. {3. and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 3.0 Feng Lin Road, shanghai 2.0032

＜正＞Flavonoids play important roles in recruitment of pollinators and seed dispersers, protecting plants against various stresses, being signals between plants and microbes and regulating auxin transport. They also ... 详细信息

关键词： Gene Clone Yellow cotyledon Reduced Accumulation Anthocyanins Arabidopsis

新世纪微生物学者的一项重要任务——未培养微生物的分离培养

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生物工程学报 2004年第5期20卷 641-645页

作者：焦瑞身中国科学院上海生命科学研究院植物生理生态研究所上海200032

扼要介绍近年来有关分离培养未培养的微生物 (Unculturablemicroorganisms)、扩大生态环境微生物多样性的认识等方面取得的重要进展。首先 ,是采用非常规的 ,甚至认为有毒性的电子传递物质 ,获得了多种新生理型(physiotypes)的纯种微生... 详细信息

扼要介绍近年来有关分离培养未培养的微生物 (Unculturablemicroorganisms)、扩大生态环境微生物多样性的认识等方面取得的重要进展。首先 ,是采用非常规的 ,甚至认为有毒性的电子传递物质 ,获得了多种新生理型(physiotypes)的纯种微生物。另外 ,在多种生态域中分离出非同寻常的微生物 ,扩大了这些生境微生物的多样性的视野 ,如普遍存在于大洋的浮游细菌SAR11类群 ,其微小菌体呈半月形 ,在海水中细菌浓度可达 (10 5～ 10 6 ) mL ,基因组估计为 1 5 4Mb。更值得注意的是从极端高温环境分离的嗜热纳米古细菌属 (Nanoarchaeota) ,其基因组仅有5 0 0kb ,是已知原核生物的最小者。应重视的是在分离这些多样性微生物的过程中所发展的新型培养技术 ,如微滴胶囊化法和扩散小室法都是具有革命性的方法 ,既有通用性 ,又无需昂贵设备。

关键词：微生物学者微生物多样性生态环境生理型嗜热纳米古细菌

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Control of Rice Grain Filling and Duplication and Independent Selection of the Cell Wall Invertase Genes

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Control of Rice Grain Filling and Duplication and Independen...

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Ertao Wang, Qun Li, Zuhua He* institute of plant {2. and {3., shanghai institutes for biological sciences, chinese academy of sciences, shanghai 2.0032. China.

＜正＞Rice grain-filling, a process controlling the weight of each grain after pollination, has been a bottleneck in

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A proteomic approach to analyzing responses of callus cells and root tip cells of Arabidopsis thaliana to altered gravity

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A proteomic approach to analyzing responses of callus cells ...

2.06年拟南芥研究学术研讨会

作者： Huiqiong Zheng, Hui Wang institute of plant {2. and {3., shanghai institutes for biological sciences, chinese academy of sciences, 3.0 Fenglin Road, shanghai 2.0032. China

＜正＞Gravity has a profound influence on plant growth and development. Removal the influence of gravitational acceleration by spaceflight may lead to a wide range of cellular changes in plant. Whole seedling, that germinated and grown on clinostats （simulated weightlessness）, showed an absence of gravitropism. At the cellular level, clinostat treatment has specific effects on plant cells, such as alterations in cell wall composition, increased production of heat-soluble proteins, changes in cellular energy metabolism, facilitating a uniform distribution of plastids （amyloplasts）, and an increase in the number and volume of nucleoli.

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Transcriptional, post-transcriptional and post-translational gene regulations during leaf polarity formation

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Transcriptional, post-transcriptional and post-translational...

2.06年拟南芥研究学术研讨会

作者： Hai Huang National Laboratory of plant Molecular Genetics, shanghai {2. of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 2.0032. China

＜正＞Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shoot apical meristem （SAM）. Recent studies on leaf morphogenesis have led to significant progress towards understanding the genetic and molecular control of leaf polarity establishment. In particular, several transcription factors have been found to play critical roles in promoting adaxial or

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