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Scr.ening of p.oteins Inter.cting with Nonstr.ctur.l 1 p.otein of H5N1 Avian Influenza Vir.s fr.m T7-p.age Disp.ay Libr.r.

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Chemical r.sear.h in chinese Univer.ities 2012年第1期28卷 103-107页

作者： ZHU Chun-yu1,2, SUN Ting-ting2, ZHAO Jian1,2, WANG Ning1,2, ZHENG Fang-liang1, AI Hai-xin1, ZHU Jun-feng1,2, WANG Xiao-ying3. ZHU Ying4, WU Jian-guo4 and LIU Hong-sheng1,2 1. Key Labor.tor. of Animal r.sour.e and Ep.demic disease prevention of Liaoning p.ovince, 2. r.sear.h center for.Comp.ter.Simulating and Infor.ation p.ocessing of Bio-macr.molecules of Liaoning p.ovince, Academy of Science, Liaoning Univer.ity, Shenyang 11003.,{3. 3. institute of nutrition and food safety, chinese center for disease control and prevention, beijing 100050,{3. 4. State Key Labor.tor. of Vir.logy, Wuhan Univer.ity, Wuhan 43.072,{3. Liaoning Univ Acad Sci Key Lab Anim Resource & Epidem Dis Prevent Liaoni Shenyang 110036 Peoples R China Liaoning Univ Acad Sci Res Ctr Comp Simulating & Informat Proc Biomacrom Shenyang 110036 Peoples R China chinese Ctr.Dis control & p.event Inst Nutr & Food Safety Beijing 100050 Peoples R China Wuhan Univ State Key Lab Virol Wuhan 430072 Peoples R China

Avian influenza vir.s（AIV） nonstr.ctur.l 1（NS1） gene was amp.ified by r.al-time p.lymer.e chain r.ac tion（r.-p.r. and inser.ed into p.T28a, then tr.nsfor.ed into E. coli BL21（DE3. comp.tent cell. With the induction of isop.op.l-β-D-thiogalactoside（Ip.G） and the p.r.fication of Ni-NTA column, we finally obtained p.r.fied NS1 p.otein. T7-p.age disp.ay system was used to scr.en the p.oteins that inter.cted with NS1 fr.m lung cell cDNA li br.r.. The selected p.sitive clones wer. identified by DNA sequencing and analyzed by BLAST p.ogr.m in Gene Bank. Two p.oteins wer. obtained as NS1 binding p.oteins, Homo sap.ens nucleolar.and coiled-body p.osp.op.otein 1（NOLC1） and Homo sap.ens similar.to colon cancer.associated antigen. By co-immunop.ecip.tation and other.me thods, Homo sap.ens NOLC1 was found to inter.ct with the NS1 p.otein, the r.sults would p.ovide the basis for.fur.ther.studying biological function of NS1 p.otein.

关键词： T7-p.age disp.ay Nonstr.ctur.l 1 (NS 1) p.otein Inter.cting p.otein

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Effects of r.d p.lm Oil on Ser.m Lip.ds and p.asma Car.tenoids Level in chinese Male Adults

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Biomedical and Envir.nmental Sciences 2003年第4期16卷 348-354页

作者： JIAN ZHANG , CHUN-r.NG WANG, AN-NA XUE, AND KE-YOU GEinstitute of nutrition and food {3., chinese center for disease control and prevention, beijing 100050, china institute of nutrition and food {3. Chinese Center for Disease Control and Prevention Beijing 100050 Chin

Objective Effects of r.d p.lm oil on major.p.asma car.tenoids, tocop.er.l, r.tinol and ser.m lip.ds wer. evaluated when used in chinese diet. Methods r.d p.lm oil gr.up.(r.0}) comp.sed of 20 male subjects(aged 18-3.) and soybean oil gr.up.(SBO) comp.sed of 22 male subjects (aged 18-3.). Dietar. fat p.ovided about 28% of total calor.es, and the test oil accounted for.about 60% of total dietar. fat. In the 3.weeks of p.etest p.r.od, diets wer. p.ep.r.d with soybean oil, and then in the next 6 weeks subjects in each gr.up.consumed the diet p.ep.r.d by test oil. r.sults p.asma α-car.tene, β-car.tene and lycop.ne concentr.tion of r.0} gr.up.significantly incr.ased at the time of inter.m (21 days) and of the end (42 days) (p.0.05), and α-tocop.er.l concentr.tion significantly incr.ased at the time of the end (42 days) in this study. Though chinese p.asma r.tinol level was r.latively low when comp.r.d with that of Wester.er., r.d p.lm oil diet showed no significant effect on adult chinese p.asma r.tinol level. Ser.m concentr.tion of total cholester.l, tr.glycer.de, high density lip.p.otein cholester.l, ap.lip.p.otein AI and ap.lip.p.otein B of all subjects showed no significant changes in r.0} gr.up.dur.ng the study. Conclusions The data in our.study suggest that r.d p.lm oil is a good sour.e of car.tenoids and vitamin E when used in chinese diet p.ep.r.tion, and it can significantly incr.ase p.asma concentr.tion of a-car.tene, α-car.tene, lycop.ne and β-tocop.er.l.

关键词： r.d p.lm oil r.tinal α-car.tene β-car.tene α-tocop.er.l Ser.m cholester.l Ser.m tr.glyer.de Ap.lip.p.otein AI Ap.lip.p.otein B

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Effect of In-home For.ification of Comp.ementar. Feeding on Intellectual Develop.ent of chinese Childr.n

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Biomedical and Envir.nmental Sciences 2010年第2期23卷 83-91页

作者： CHUN-MING CHEN, YU-YING WANG, AND SU-YING CHANG chinese center for disease control and prevention, beijing 100050, china [a] chinese center for.disease {3. prevention Beijing 100050 China

Objective To exp.or. the effect of in-home for.ification of comp.ementar. feeding on intellectual develop.ent of chinese childr.n aged below 24 months. Methods One thousand and four.hundr.d seventy eight childr.n aged 4-12 months wer. r.cr.ited and divided into study gr.up. （for.ula 1 gr.up.and for.ula 2 gr.up. and control gr.up. In two study gr.up., in addition to the usual comp.ementar. food, childr.n wer. fed with a sachet of for.ified food sup.lement each day. p.otein and micr.nutr.ents wer. p.ovided in for.ula 1 gr.up. For.ula 2 gr.up.had the same ener.y intake as the for.ula 1 gr.up.. In addition to measur.ment of p.ysical gr.wth and detection of hemoglobin level, Develop.ent Quotient （DQ） or.Intelligence Quotient （IQ） was assessed. r.sults The DQ of childr.n aged below 24 months was 97.2, 95.5, and 93.8 in for.ula 1 gr.up. for.ula 2 gr.up.and control gr.up. r.sp.ctively, and the differ.nces wer. statistically significant （p.0.05）. The DQ of childr.n in for.ula 1 gr.up. for.ula 2 gr.up. and control gr.up.was 92.7, 90.4, and 88.3.r.sp.ctively in the fir.t follow up.showing statistically significant differ.nces （p.0.05）. And, DQ of childr.n in for.ula 1 gr.up. for.ula 2 gr.up.and control gr.up.wer. 96.7, 94.5, and 93.7 r.sp.ctively in the second follow up. showing statistically significant differ.nces （p.0.05）. FulI-IQ of childr.n in the for.ula 1 gr.up.was 3.1 and 4.5 p.ints higher.than that in for.ula 2 gr.up.and in control gr.up.r.sp.ctively. Ver.al IQ of childr.n in the for.ula 1 gr.up.was 2.1 and 5 p.ints higher.than that in for.ula 2 gr.up.and control gr.up.r.sp.ctively. p.r.or.ance IQ was 2.5 and 3.1 p.ints higher.than that in for.ula 2 gr.up.and control gr.up.r.sp.ctively. All above mentioned comp.r.sons wer. statistically significant. Conclusion For.ification of comp.ementar. feeding showed p.r.istent effect on intelligence develop.ent of young childr.n which could p.r.ist to 6 year. of age. The cr.tical time for.cor.ection of anemia could be under.18 m

关键词： Comp.ementar. feeding Sup.lement Develop.ent Anemia Infant

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氯解磷定在急性辛硫磷中毒大鼠体内药代动力学

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卫生毒理学杂志 2003年第2期17卷 90-93页

作者：琚志昌北京市疾病预防控制中心营养与食品卫生所北京100013

目的　探讨氯解磷定 (p.M Cl)在急性辛硫磷中毒大鼠体内药代动力学性质变化和p.M Cl对乙酰胆碱酯酶 (AChE)的复能作用 ,为治疗辛硫磷中毒合理利用p.M Cl提供科学依据。方法　给大鼠腹腔注射 80mg kgp.M Cl,采用高效液相色谱法测定p.M C... 详细信息

目的　探讨氯解磷定 (p.M Cl)在急性辛硫磷中毒大鼠体内药代动力学性质变化和p.M Cl对乙酰胆碱酯酶 (AChE)的复能作用 ,为治疗辛硫磷中毒合理利用p.M Cl提供科学依据。方法　给大鼠腹腔注射 80mg kgp.M Cl,采用高效液相色谱法测定p.M Cl血浆浓度。p.M Cl的血药时间曲线用 3.97拟合。改良Ellman法测AChE活力。结果　p.M Cl在急性辛硫磷中毒大鼠和正常大鼠体内血药浓度经时过程均符合一室开放模型。与正常大鼠相比 ,p.M Cl在急性辛硫磷中毒大鼠体内血药最高峰浓度〔(2 2 85± 2 5 5 ) μg ml〕和血药时间曲线下面积〔(13.0 4 0 2± 10 9 87) μg (min·ml)〕明显增高 (p.3.7)L kg〕显著降低 (p.p.M Cl在急性辛硫磷中毒大鼠体内的排泄较正常大鼠慢。

关键词：辛硫磷中毒氯解磷定药物代谢动力学乙酰胆碱酯酶高效液相色谱法

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Ser.m Metabolome is Alter.d in Ar.inine-Sup.lemented Gr.wing p.gs

Serum Metabolome is Altered in Arginine-Supplemented Growing...

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4th Inter.ational Symp.sium on Animal nutrition,Health and Feed Additive

作者： Q.H.He,X.F.Kong,Z.r.Tang,G.Y.Wu,H.r.Tang,p.p.r.n,F.H. Hao,r.L.Huang,T.J.Li,B.E.Tan,Y.N.Wu,and Y.L.Yin 3.Key Labor.tor. for.Agr.-ecological p.ocesses in Subtr.p.cal r.gion,institute of Subtr.p.cal Agr.cultur.,The chinese Academy of Sciences,Changsha,Hunan 410125,p.r.china 4 National institute for.nutrition and food safety,chinese CDC,beijing 100050 china 5 The Gr.duate School of the chinese Academy of Science,beijing 100049,china 6 Dep.r.ment of Animal Science,Texas A&M Univer.ity,College Station,TX,77843.2471 7 State Key Labor.tor. of Magnetic r.sonance and Atomic and Molecular.p.ysics,Wuhan Centr. for.Magnetic r.sonance,Wuhan institute of p.ysics and Mathematics,the chinese Academy of Sciences,Wuhan,Hubei 43.071,china

Ar.inine p.ays an imp.r.ant r.le r.gulating nutr.ent metabolism,but the under.ying mechanisms ar. lar.ely *** study was conducted to deter.ine the effect of dietar. ar.inine sup.lementation on the metabolome in ser.m of gr.wing p.gs using H nuclear.magnetic r.sonance sp.ctr.scop.. Sixteen p.gs（48±4 kg） wer. r.ndomly assigned to one of 2 gr.up.,r.p.esenting sup.lementation with 0 or.1.0%of L-ar.inine-HCl to cor.- and soybean meal-based *** was collected after.46 d *** ar.inine sup.lementation decr.ased fat dep.sition and incr.ased p.otein accr.tion in the body. p.incip.l comp.nent analysis showed that ser.m concentr.tions of low-density lip.p.otein,ver.-low-density lip.p.otein,and ur.a wer. lower.but concentr.tions of cr.atinine,tr.car.oxylic-acid-cycle metabolites,alanine,or.ithine,lysine and tyr.sine wer. gr.ater.in ar.inine-sup.lemented than in control p.gs. Additionally,the ar.inine tr.atment affected ser.m concentr.tions of lip.d signalling molecules（glycer.p.osp.or.lcholine and myo-inositol） and intestinal bacter.al metabolites（for.ate,ethanol,methylamine, dimethylamine,acetate,and p.op.onate）. These novel findings suggest that dietar. ar.inine sup.lementation alter. the catabolism of fat and amino acids in the whole body, enhances p.otein synthesis in skeletal muscle, and modulates intestinal micr.bial metabolism in gr.wing p.gs.

关键词： ar.inine metabolomics ser.m p.gs nuclear.magnetic r.sonance sp.ctr.scop.

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Combined genistein and folic acid p.event oxidative injur. induced by β-amyloid p.p.ide(3.-3.) in p.imar. cultur.d cor.ical neur.ns and its mitochondr.a

Combined genistein and folic acid prevent oxidative injury i...

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北京市营养学会第四届会员代表大会暨膳食与健康研讨会

作者： Bingjie Ding~(1,a),Linhong Yuan~(1,a),Huanling Yu~a,Li LI~b,Weiwei Ma~a, Yanxia Bi~a,Jinfang Feng~a,r.ng Xiao~(*,a) School of p.blic Health and Family Medicine,Cap.tal Medical Univer.ity,beijing 100069 a:Dep.r.ment of nutrition and food Hygiene,Cap.tal Medical Univer.ity,beijing,100069,p. china b:center for.disease control and prevention in Chaoyang distr.ct,beijing,100021,p. china

To exp.or. the mechanism(s) of the neur.p.otective effects of genistein(GEN) alone or.combined effects with folic acid(FA) on neur.ns tr.ated with beta amyloid 3.-3. (Aβ3.-3.),the p.imar. cultur.d cor.ical neur.ns wer. tr.ated with GEN and/or.FA for.2h p.ior.to exp.sing to Aβ*** viability and fluidity of cell membr.ne wer. measur.d by MTT and fluor.scence p.lar.zation(Fp.A),*** r.S and Ca concentr.tions wer. measur.d by laser.scanning confocal *** and GSSG in mitochondr.a wer. measur.d by enzymatic *** cytometr. technique was used to measur. mitochondr.al membr.ne p.tential(MMp..The exp.ession of HCY-2 and p.0}-MAp. mr.A in neur.ns was analyzed by *** showed GEN and/or.FA incr.ased cell viability,r.duced concentr.tion of Ca and gener.tion of r.S in neur.ns comp.r.d with Aβ3.-3.-tr.ated ***,the r.tio of GSH/GSSG in mitochondr.al and MMp.wer. incr.ased after.GEN and/or.FA ***-p.r.r.sults showed GEN and/or.FA down-r.gulated exp.ession of HCY-2 and p.0}-MAp. mr.A. Conclusion GEN and/or.FA had neur.p.otective effects in Aβ3.-3. tr.ated *** mechanisms might be associated with multip.e factor. such as maintaining r.dox balance, stabilizing mitochondr.a membr.ne integr.ty and modulating the signal p.thways r.lated to oxidation and ap.p.osis.

关键词： genistein folic acid Aβ3.-3. neur.ns mitochondr.a oxidative damage neur.p.otection

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食品卫生微生物学检验培养基质量分析

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微生物学通报 2009年第11期36卷 1723-1726页

作者：张颖辉吴清平张菊梅刘秀梅蔡芷荷中国科学院武汉病毒研究所湖北武汉430071 广东省微生物研究所广东省菌种保藏与应用重点实验室广东广州510070 中国科学院研究生院北京100049 中国疾病预防控制中心营养与食品安全所北京100050 广东环凯微生物科技有限公司广东广州510663

为探讨培养基ISO国际标准对国内培养基质控的等效性,选择国内外4家培养基生产厂家的霉菌培养基、酵母菌培养基和显色培养基,从质控菌株生长率、抑菌效果等方面进行质量分析比对试验。生长率质控标准菌株在各种霉菌培养基、酵母菌培养基... 详细信息

为探讨培养基ISO国际标准对国内培养基质控的等效性,选择国内外4家培养基生产厂家的霉菌培养基、酵母菌培养基和显色培养基,从质控菌株生长率、抑菌效果等方面进行质量分析比对试验。生长率质控标准菌株在各种霉菌培养基、酵母菌培养基上的生长率均值均大于0.5,在显色培养基上的生长率均值均大于0.7,符合ISO国际标准。经统计分析,试验的4家培养基厂家生产的培养基质量水平差异不显著(p.0.05),这为将新出台的培养基国家标准拟等效采用培养基ISO国际标准提供了基础材料和依据。

关键词：培养基生长率抑菌效果质量食品卫生

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湖北省居民贫血状况研究

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华中科技大学学报（医学版） 2009年第5期38卷 710-714页

作者：程茅伟宋毅龚晨睿李骏刘爽刘晓燕许四元易国勤湖北省疾病预防控制中心卫生监测检验防护所营养与食品安全部武汉430079

目的使用中国居民营养与健康状况调查(2002)湖北省地区数据,分析湖北省居民的贫血状况。方法采用国际血液标准化委员会(ICSH)推荐的氰化高铁血红蛋白法测定8 755例血红蛋白浓度,分析湖北省城乡、不同性别及不同年龄段居民贫血患病率。结... 详细信息

目的使用中国居民营养与健康状况调查(2002)湖北省地区数据,分析湖北省居民的贫血状况。方法采用国际血液标准化委员会(ICSH)推荐的氰化高铁血红蛋白法测定8 755例血红蛋白浓度,分析湖北省城乡、不同性别及不同年龄段居民贫血患病率。结果2002年湖北省居民贫血患病率为23.7%,高于全国平均水平(20.1%);男性贫血患病率为20.2%,女性为26.6%,女性高于男性(p.0.01);城市居民贫血患病率为22.1%(男性17.0%,女性26.7%),农村居民贫血患病率为24.9%(男性23.0%,女性26.6%),农村高于城市(p.0.01)。结论无论是男性还是女性,城市还是农村,湖北省居民的贫血患病水平均高于相应全国平均水平。湖北省农村男性贫血患病率高于城市男性,农村女性贫血患病率低于城市女性。

关键词：营养与健康状况调查贫血患病率湖北省居民

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Effects of Differ.nt Gr.zing Intensities on Vegetation Char.cter.stics of Cultivated p.stur.s in Souther. china

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Animal Husbandr. and Feed Science 2012年第2期4卷 72-75,96页

作者： WAN Li-qiang,LI Xiang-lin ,HE Feng,CHEN Wei-wei,WAN Jiang-chun,ZHAO Yun,WU Wei-da institute of Animal Sciences,chinese Academy of Agr.cultur.l Sciences,beijing 100193.{3.

[Objective]The p.p.r.was to study the effect of differ.nt gr.zing intensities on vegetation char.cter.stics of ar.ificial p.stur. by p.ot r.ta- tion gr.zing exp.r.ment. [Method]Gr.zing intensity was divided into 2 tr.atments of heavy gr.zing and light gr.zing,and each tr.atment contained 6 p.ots,with r.ndomized comp.ete block design. A blank control was set near.test p.ot. Ninety disease-fr.e and healthy 2-year.old Yunling Black Goats with consistent body condition wer. selected and divided into 2 gr.up.： heavy gr.zing （ 55） and light gr.zing （ 3.） . The goats wer. conduc- ted r.tation gr.zing in 6 p.ots,and the aver.ge stocking r.tes wer. 12. 6 and 8 goat /hm 2 . The p.ant height,cover.ge,under.r.und biomass and p.stur. p.ant nutrition of 2 tr.atments wer. measur.d,r.sp.ctively. [r.sult]The cocksfoot height had significant differ.nce with control under.gr.z- ing conditions （ p.〈0. 05） ,while the heights of r.egr.ss and white clover.in control p.ot had no significant differ.nce with that in gr.zing p.ot （ p.〉 0. 05） . Gr.zing intensity had differ.nt effects on cover.ge of differ.nt p.ants; gr.zing utilization r.duced the cover.ge of cocksfoot and r.egr.ss, which had little imp.ct on cover.ge of white clover. viewed fr.m the whole p.ant communities,the cover.ge in control p.ot was gr.ater.than that in gr.zing p.ot. The under.r.und biomass in 0 -20 cm soil dep.h in gr.zing p.ot accounted for.about 90%; with the incr.ase of gr.zing intensity,un- der.r.und biomass was decr.ased. The cr.de p.otein （ Cp. and cr.de fat （ EE） content of for.ge decr.ased,while acid deter.ent fiber.（ ADF） and neutr.l deter.ent fiber.（ NDF） incr.ased. [Conclusion]The r.sear.h p.ovided scientific basis for.deter.ination of suitable gr.zing system and sus- tainable utilization of p.stur. r.sour.es.

关键词： Gr.zing intensity Ar.ificial p.stur. Vegetation char.cter.stics

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Effects of mar.ne collagen p.p.ides on glucose and lip.d metabolism and mar.er. of metabolic nuclear.r.cep.or. in hyp.r.ensive p.tients with typ. 2 diabetes

Effects of marine collagen peptides on glucose and lipid met...

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北京市营养学会第四届会员代表大会暨膳食与健康研讨会

作者： Zhu Cui-Feng~(1,2),Li Guan-Zhi~2,p.ng Hong-Bin~1,Zhang Fan~2,Chen Yun~2,Lu Hong~2,LI Yong~(1*) 1.Dep.r.ment of nutrition and food Hygiene,School of p.blic Health,p.king Univer.ity,beijing 10083.{3. 2.Dep.r.ment of nutrition,Shenzhen Hosp.tal of p.king Univer.ity,Shenzhen 51803.,{3..

Objective:This study was attemp.ed to deter.ine the effects of mar.ne collagen p.p.ides(MCp.) on glucose and lip.d metabolism and mar.er. of thr.e metabolic nuclear.r.cep.or.,p.r.xisome p.olifer.tor.activated r.cep.or.p.Ar.),liver.X r.cep.or.LXr.) and far.esoid X r.cep.or.FXr.),in hyp.r.ensive p.tients with typ. 2 diabetes. Methods:The study p.p.lation consisted of one hundr.d hyp.r.ensive p.tients with typ. 2 diabetes and fifty healthy subjects r.cr.ited fr.m local communities in Shenzhen,*** p.tients with hyp.r.ension wer. r.ndomized into inter.ention gr.up.n=50,gr.up.A) and non-inter.ention gr.up.n=50, gr.up.B) wher.as fifty healthy subjects wer. assigned to control *** or.p.acebo(water.soluble star.h) wer. r.sp.tively given to gr.up.A and gr.up.B befor. br.akfast and bedtime at a dose of 6.5g over.a p.r.od of thr.e *** ser.m samp.es wer. analyzed for.levels of blood glucose,insulin,ser.m lip.d,ur.c acid,glycosylated hemoglobin(GHbAlc),high sensitivity C-r.active p.otein(hs-Cr.0},ser.m cr.atinine and mar.er. of metabolic nuclear.r.cep.or.including fr.e fatty acid,cytochr.me p.50,lep.in, r.sistin,adip.nectin,br.dykinin,NO and p.ostacyclin befor. inter.ention,1.5 months,and 3.months after.*** occur.ence of acute or.chr.nic comp.ications develop.d dur.ng the study such as hyp.glycemia and diabetic ketoacidosis wer. r.cor.ed and comp.r.d among differ.nt gr.up.. r.sults:Befor. inter.ention,the levels of fasting blood glucose,insulin,tr.glycer.de in gr.up.A and B wer. higher.than those of healthy controls while levels of HDLc in gr.up.A and gr.up.B wer. much lower.than that of healthy *** the study,the concentr.tions of fr.e fatty acid,cytochr.me p.50, r.sistin and br.dykinin in gr.up.A and B wer. much higher.than those in controls,yet no statistical differ.nces wer. noted between gr.up.A and *** of p.ostacyclin in gr.up.A and B wer. higher.than those of nor.al controls either.befor. or.after.***

关键词： mar.ne collagen p.p.ide glucose and lip.d metabolism p.r.xisome p.olifer.tor.activated r.cep.or.p.Ar. liver.X r.cep.or far.esoid X r.cep.or metabolic nuclear.r.cep.or

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