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Formation of complexes of Rubisco-Rubisco activase from La^3., Ce^3. treatment spinach

Science china Chemistry 2005年第1期48卷 67-74页

作者： HONG Fashui1, LIU Chao1, ZHENG Lei1, WANG Xuefeng1, WU Kang1, SONG Weiping1, Lü Shipeng1, TAO Ye2.& ZHAO Guiwen3.1. College of Life Sciences, Suzhou Universty, Suzhou 2.5006, china 2. Synchrotron Radiation Laboratory, {2. of high {3. physics, The chinese academy of Science, beijing 10003., china 3. Chemical Department, University of Science and Technology of china, Hefei 2.0}02., china College of Life Sciences Suzhou Universty Suzhou China Synchrotron Radiation Laboratory Institute of High Energy Physics The Chinese Academy of Science Beijing China Chemical Department University of Science and Technology of China Hefei China

The complex of Rubisco and Rubisco activase from LaCl3., CeCl3. treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3., CeCl3. treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3., CeCl3. treated spinach have not only a band of Rubisco (560 kD), but also a band of about 1100 kD, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3., CeCl3.treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 3.—3. –SH per mol enzyme, 14—2. –SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3., CeCl3.-treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3., CeCl3. treated spinach in vivo are 1.5 and 1.9 times those of the control.

关键词： La3., Ce3., spinach, Rubisco, complex of Rubisco and Rubisco acitivase, spectral characteristics.

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Physical mechanisms of plant roots affecting weathering and leaching of loess soil

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Science china Earth Sciences 2006年第9期49卷 1002-1008页

作者： LI Yong1,2. ZHANG Qingwen1,2. WAN Guojiang3. HUANG Ronggui3. PIAO Hechun3. BAI Lingyu2.& LI Lu2.1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, institute of Soil and Water Conservation, chinese academy of Sciences, Yangling 712.00,{3. 2. institute of Agro-Environment and Sustainable Development, chinese academy of Agricultural Sciences, beijing 100081,{3. 3. institute of Geochemistry, chinese academy of Sciences, Guiyang 550002.{3. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Institute of Soil and Water Conservation Chinese Academy of Sciences Yangling China institute of Agro-Environment and Sustainable Development Chinese Academy of Agricultural Sciences Beijing China institute of Geochemistry Chinese Academy of Sciences Guiyang China

Plant roots have potential impacts on soil mineral weathering and *** objective is to understand the physical mechanisms of plant roots affecting weathering andleaching of loess soil. Root densities were measured through the method of a large-size dug profile,and transport fluxes of soil elements were determined using an undisturbed monolith soilinfiltration device on the hilly and gully regions of the chinese Loess Plateau. The results showthat the improvement effects of soil environment by plant roots are mainly controlled by the densityand weight of the fibrous roots with the diameters less than 1 mm. Plant roots have the strongereffects on soil physical properties than chemical properties. The principal components analysis(PCA) indicates that soil physical properties by plant roots account for 56.7% of variations in soilenvironment whereas soil chemical properties and pH contribute about 2..2. of the soil *** roles of plant roots in controlling soil weathering and leaching increased in the followingorder: infiltration enhancement > increase of bio-active substance > stabilization of soilstructure. The effects of plant roots on soil mineral weathering and leaching can be quantifiedusing the multiple regression models with the high prediction accuracies developed in this study.

关键词： plant roots, soil weathering and leaching rates, soil environment field, principal components analysis (PCA), chinese Loess Plateau.

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Modeling and verifying Web services driven by requirements:An ontology-based approach

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Science in china(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3.JIN ZHi1,2.& WU Budan1,4 1. academy of Mathematics and Systems Science, {2. academy of Sciences, {3. 100080, china 2. institute of Computing Technology, {2. academy of Sciences, {3. 100080, china 3. institute of Software, {2. academy of Sciences, {3. 100080, china 4. Graduate University of {2. academy of Sciences, {3. 100049, china academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing China institute of Software Chinese Academy of Sciences Beijing China institute of Computing Technology Chinese Academy of Sciences Bejing China Graduate University of chinese academy of Sciences Beijing China

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

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Effect of rock mass structure and block size on the slope stability——Physical modeling and discrete element simulation

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Science china(Technological Sciences) 2005年第Z1期48卷 1-17页

作者： LI Shihai1, LIAN Zhenzhong1,2.& J. G. Wang3.1. institute of Mechanics, {2. academy of Sciences, {3. 100080, china 2. Graduate School, {2. academy of Sciences, {3. 100060, china 3. Tropical Marine Science institute, National University of Singapore, Singapore 1192.0 institute of Mechanics Chinese Academy of Sciences Beijing 100080 China Graduate School Chinese Academy of Sciences Beijing 100060 China Tropical Marine Science institute National University of Singapore Singapore 119260 Singapore

This paper studies the stability of jointed rock slopes by using our improved three-dimensional discrete element methods (DEM) and physical modeling. Results show that the DEM can simulate all failure modes of rock slopes with different joint configurations. The stress in each rock block is not homogeneous and blocks rotate in failure development. Failure modes depend on the configuration of joints. Toppling failure is observed for the slope with straight joints and sliding failure is observed for the slope with staged joints. The DEM results are also compared with those of limit equilibrium method (LEM). Without considering the joints in rock masses, the LEM predicts much higher factor of safety than physical modeling and DEM. The failure mode and factor of safety predicted by the DEM are in good agreement with laboratory tests for any jointed rock slope.

关键词： rock masses, slope stability, DEM simulation, block size effect, joints configuration effect, limit equilibrium method.

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Relationships between tree-ring width index and NDVI of grassland in Delingha

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chinese Science Bulletin 2006年第9期51卷 1106-1114页

作者： HE Jicheng1,3.& SHAO Xuemei1,2.. institute of Geographical Sciences and Natural Resources Research, {2. academy of Sciences, {3. 100101, china 2. institute of Tibetan Plateau Research, {2. academy of Sciences, {3. 100085, china 3. Graduate School of {2. academy of Sciences, {3. 10003., china institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China Graduate School of chinese academy of Sciences Beijing China institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing China

Using five well-replicated Qilian juniper (Sabina przewalskii Kom.) tree-ring width index se- ries, monthly normalized difference vegetation index (NDVI) of grassland, and climatic data from 1982.to 2.01, the relationships between tree-ring width index, NDVI of grassland, and climatic data were analyzed firstly. Then, the relationship between tree-ring width index and NDVI of grassland was explored. The re- sults showed that: (1) Temperature and precipitation in June influenced tree-ring width index and NDVI of grassland deeply in Delingha. (2. There were sig- nificant relationships between five tree-ring width index series (DLH1-DLH5) and monthly NDVI of grassland from June to September, with the most significant relationship being between tree-ring width index series and NDVI of grassland in August. (3. The PC1 (the first principal component derived from DLH1-DLH5 series) exhibited good agreement with monthly NDVI of grassland in the grass growth sea- son (from June to September) and the averaged NDVI in the growth season, which was attributed to their common responses to water-supply limit in Delingha. This study may allow an increase in studying the past dynamics of grassland in Delingha in that the variation of grassland NDVI during the last millennium has been reconstructed from PC1.

关键词：圆柏祁连山 NDVI 草原年轮宽度指数

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Approaches to improving critical current density of YBCO superconducting thick films deposited by electrophoresis

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Science china(physics,Mechanics & Astronomy) 2004年第2期47卷 165-172页

作者： ZHU Yabin1,2. ZHOU Yueliang2. LIU Zhen2. WANG Shufang2. CHEN Zhenghao2. L?Huibin2. YANG Guozhen2. XIAO Ling3. REN Hongtao3. JIAO Yulei3.& ZHENG Minghui3.1. Department of physics, College of Science, beijing Jiaotong University, beijing 100044,{3. 2. Laboratory of Optical physics, institute of physics and Center for Condensed Matter physics, {12. academy of Sciences, beijing 100080,{3. 3. General Research institute for Nonferrous Metals, beijing 100088,{3. Correspondence should be addressed to Zhu Yabin (email: ybzhu@***) Department of physics College of Science Beijing Jiaotong University Beijing China Laboratory of Optical physics institute of physics and Center for Condensed Matter physics Chinese Academy of Sciences Beijing China General Research institute for Nonferrous Metals Beijing China

Fabrication of YBCO superconducting thick films by electrophoresis has been widely reported. However, the value of critical current density (J c) is far from what the practical application requires. The superconducting thick films are obtained using top-seeded melt growth method with different proportional Y2.aCuO5 (Y2.1) addition. high-pressure oxygen has been used in the annealing process to improve the uniformity of the oxygen content in the superconducting thick films. When 40mol% Y2.1 powder is added to the YBa2.u3.7–5 (Y12.1} powder,J c for the superconducting thick film by approaches described above reaches 7.008×103.A/cm2.(77 K, 0 T), which is greater than the value ever reported.

关键词： YBCO thick films, electrophoretic deposition, melt-textured.

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Application of the moist vorticity vector in the analysis of a heavy rainfall event in North china

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Progress in Natural Science:Materials International 2007年第12期17卷 1457-1465页

作者： Zhao Yu1,2.3. Wang Jianguo4 and Xue Deqiang2.1. Laboratory of Cloud-Precipitation physics and Severe Storms, {2. of Atmospheric physics, {3. academy of Sciences, beijing 10002., china 2. Shandong Provincial Meteorological Observatory, Jinan 2.003., china 3. Graduate University of {3. academy of Sciences, beijing 10003., china 4. Shandong Provincial Meteorological Bureau, Jinan 2.003., china) Laboratory of Cloud-Precipitation physics and Severe Storms Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Shandong Provincial Meteorological Observatory Jinan 250031 China Graduate University Chinese Academy of Sciences Beijing 100039 China Shandong Provincial Meteorological Bureau Jinan 250031 China

The moist vorticity vector (MVV), defined as (ζa×▽qv)/ρ introduced by Gao et al. is used to study a heavy rainfall event in North china. The MVV has zonal, meridional and vertical components in a three-dimensional framework. Analysis of domain-mean and mass-integrated quantities shows that the variation of the vertical component of the MVV closely follows the variation of the cloud hydrometeors, with a linear correlation coefficient of 0.95, indicating that the vertical component of the MVV is strongly associated with midlatitude convection. Further analysis shows that the vertical component of the MVV reflects the interaction between the horizontal vorticity and the horizontal specific humidity gradient, which can associate dynamic and thermodynamic processes with cloud microphysical processes. Thus, this study can help to understand how the interaction between circulations and clouds aids the development of convection, and the MVV can be used to trace the development and evolution of heavy rainfall.

关键词： moist vorticity vector heavy rainfall modeling study.

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A template of rat brain based on fMRI T2. imaging

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Progress in Natural Science:Materials International 2005年第6期15卷 502-506页

作者： HU Zhenghui1, WU Yigen2. WANG Xiaochuan3. WANG Jianzhi3. CHEN Feiyan1 and TANG Xiaowei1*(1. Department of {2. and Bio-X Laboratory, Zhejiang University, Hangzhou 3.002., china 2. institute of {3. energy {2. and Key Laboratory of Nuclear Analysis Techniques, chinese academy of Sciences,beijing 10003., china 3. Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 43.03., china) Department of physics and Bio-X Laboratory Zhejiang University Hangzhou 310027 China Key Laboratory of Nuclear Analysis Techniques Institute of High Energy Physics Chinese Academy of Sciences Beijing 100039 China Department of Pathophysiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The development of functional magnetic resonance imaging (fMRI) technology has made it possible to carry out functional brain imaging experiments in small animals. Usually, group data is required to form the assessment of population, which can not only increase the sensitivity of the overall experiment, but also allow the generalization of the conclusion to the whole population. In order to average the signals of functional brain images from different subjects, it is necessary to put all the mapping images into the same standard space (template image). However, up to now, most animal brain templates remain unavailable and it must be done by ourselves. In this study, a template image based on the brains of eight male Wistar rats is obtained, and it is successfully used in our present Alzheimer disease (AD)-like rat model studies as template for spatially normalizing images to the same stereotaxical space. The fMRI results processed with statistical parametric mapping (SPM99) software are in agreement with the results from immunohistochemical experiment, which proves that this method is universally applicable to the pathologic models of other small animals and to human brain lesion studies.

关键词： fMRI animal model atlas template normalization T*2.

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Environmental characteristics of Mid-Holocene recorded by lacustrine sediments from Lake Daihai, north environment sensitive zone, china

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Science china Earth Sciences 2006年第9期49卷 968-981页

作者： SUN Qianli1,2. ZHOU Jie1, SHEN Ji3. CHEN Peng1,2. WU Feng1,2.& XIE Xiuping1,2.1. State Key Lab of Loess and Quaternary Geology, institute of Earth Environment, {2. academy of Sciences, Xi’an 710075,{3. 2. Graduate University of {2. academy of Sciences, beijing 10003.,{3. 3. Nanjing institute of Geography and Limnology, {2. academy of Sciences, Nanjing 2.0008,{3. State Key Lab of Loess and Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences Xi’an China Graduate University of chinese academy of Sciences Beijing China Nanjing institute of Geography and Limnology Chinese Academy of Sciences Nanjing China

Climate proxies, such as total organic carbon and nitrogen (TOC, TN), carbonate content (CaCO3., grain-size and pollen of the sediment core retrieved from enclosed Lake Daihai which lies in the north china environment sensitive zone are analyzed to reconstruct the environment evolution of the area based on {2.-resolution radiocarbon chronology. The results reveal that the TOC and TN contents of the sediments correlate well with pollen percentage and pollen flux variations during the Holocene, and both reach their peak values simultaneously at 6.7―3.5 ka BP (calendar age, 7.6―3.6 ka BP). Since 6.7 ka BP, both the CaCO3.and organic matter contents of the core have simultaneous variations, and their {2. values also occur during 6.7―3.5 ka BP. While during 9.0―6.7 ka BP (calendar age, 10―7.6 ka BP) relatively lower level of organic matter content and pollen flux corre- spond to the {2.er level of carbonate content. The above relations suggest that during 6.7―3.5 ka BP, the productivity and effective precipitation were greatly improved in the lake drainage area, and this would probably strengthen the hydrodynamic conditions, enhancing organic matter, pollen and carbonate inputs from terrestrial sources. Such processes would account for the enrichment of both organic matter and carbonate in the sediments. While during 9.0―6.7 ka BP, the lower level of or- ganic matter, pollen flux but {2. carbonate content show depressed productivity and declined vege- tation coverage. The {2.er carbonate content at this stage would have probably resulted from the {2.er evaporation ratio of the lake water under relatively drier climate conditions. Therefore, it is in- ferred that during 6.7―3.5 ka BP, the climate was more humid with abundant rainfalls and vegetation was more flourishing in the Lake Daihai area. This can be seen as Holocene Climate Optimum (HCO). As a result, this has evident discrepancies with the traditional notion that the HCO occurs at Early Holocene or early Mid

关键词： Lake Daihai, Mid-Holocene, lacustrine sediments, environmental characteristics.

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Modeling of elastic wave propagation on a curved free surface using an improved finite-difference algorithm

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Science china(physics,Mechanics & Astronomy) 2004年第5期47卷 633-648页

作者： WANG Xiuming1,3.& ZHANG Hailan2.1. CSIRO Petroleum, ARRC, PO BOX 113., Technology Park, Bentley, WA 6102. Australia 2. {2. of Acoustics, chinese academy of Sciences, {3. 100080, china 3. Present address: {2. of Acoustics, chinese academy of Sciences, {3. 100080, china CSIRO Petroleum ARRC Bentley Australia institute of Acoustics Chinese Academy of Sciences Beijing China

Based on existing direct and imaging methods of a staggered finite-difference scheme, an improved algorithm for staggered finite-difference is proposed to implement rugged topographic free boundary conditions. This method assumes that the free surface can be implemented with horizontal and vertical free surface segments and their corners; the free surface passes through the grid points of shear stress components, instead of the normal stress components. Imaging is carried out for stress components in both horizontal and vertical directions, thus increasing the accuracy. To update particle- velocities, imaging and updating are first performed in the horizontal direction, and then in the vertical direction. The numerical results for elastic flat horizontal free surface with the imaging method and those for flat free surfaces of various slope angles with the proposed method are compared, and are shown to be in good agreement. The advantage of the proposed method is that only the stresses are dealt with in implementing the free surface into the staggered algorithm, which improves computation efficiency.

关键词： elastic wave simulation, curved free surface, staggered finite-difference, algorithm stability.

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