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Percoll fractionation of adult mouse spermatogonia improvesgerm cell transplantation

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Asian Journal of Andrology 2004年第2期6卷 93-98页

作者： Kyu-Bom Koh Masatoshi Komiyama Yoshiro Toyama Tetsuya Adachi Chisato Mori Department of Bioenvironmental Medicine Graduate School of Medicine Chiba University Chiba 260-8670 Japan Center for Environment Health and Field sciences Chiba University Kashiwa 277-0882 Japan 不详 Department of Genomic Drug Discovery sciences graduate School of Pharmaceutical sciences Kyoto University Kyoto 606-8501 Japan Core Research for Evolutional Science and Technology Japan Science and Technology Agency Kawaguchi 332-0012 Japan

Aim: To isolate and transplant germ celis from adult mouse testes for transplantation. Methods: In order to distinguish transplanted celis from endogenous celis of recipients, donor transgenic mice expressing green fluores cent protein (GFP) were used. Germ celis were collected from the donors at 10-12.weeks of age and spermatogonia were concentrated by percoll fractionation and transplanted into recipient seminiferous tubules that had been previ ously treated with busulfan at 5 weeks of age to remove the endogenous spermatogenic celis. Results: Twenty vveeks after the transplantation, a wide spread GFP signal was observed in the recipient seminiferous tubules. The presence of spermatogenesis and spermatozoa was confirmed in sections of 12.out of 14 testes transplanted (86 %). However, when germ celis were transplanted without concentration the success rate was zero (0/9). Conclusion: Germ celis from adult mouse testes can be successfully transplanted into recipient seminiferous tubules if the cell population is rich in spermatogonia and the percoll fractionation is useful in obtaining such a cell population.

关键词： germ cell transplantation green fluorescent protein percoll fractionation spermatogenic cells mice

Sausage Appearance Defect Inspection system based on Machine Vision

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Sausage Appearance Defect Inspection system based on Machine...

2.10 chinese Control and Decision Conference

作者： Shuai Chen~(1,2.,Yue Ma~1,Haibo Shi~1 1.Shenyang Institute of Automation chinese academy of Science,Shenyang,China 2.{2. school of chinese academy of Science,Beijing,China

＜正＞A real-time sausage appearance defect inspection system is presented in this *** general hardware and software structure is presented and a model method for sausage defect detection is *** inspection system give... 详细信息

关键词： machine vision automation visual inspection minimum enclosing rectangle

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DSA检查技术临床应用专家共识

中华放射学杂志 2024年第10期58卷 995-1005页

作者：中华医学会影像技术分会中国医师协会医学技师专业委员会中国医药教育协会医学影像技术学专业委员会不详华中科技大学同济医学院附属协和医院放射科武汉430022 四川大学华西医院放射科成都610041 首都医科大学附属北京同仁医院放射科北京100730 广州市第一人民医院介入医学中心哈尔滨医科大学附属肿瘤医院影像中心

本专家共识系统地叙述DSA检查前准备、手术基本操作、相关应急预案、常用应用技术、人体各个部位的DSA成像技术参数选用、图像质量控制、注意事项及必要的辐射防护等内容。重点叙述了DSA成像时, 人体各个部位正确的造影体位, 设备的成... 详细信息

本专家共识系统地叙述DSA检查前准备、手术基本操作、相关应急预案、常用应用技术、人体各个部位的DSA成像技术参数选用、图像质量控制、注意事项及必要的辐射防护等内容。重点叙述了DSA成像时, 人体各个部位正确的造影体位, 设备的成像技术参数选用, 对比剂的注射参数优选, 以及图像质量控制和注意事项。

关键词：血管造影术, 数字减影成像技术应用规范

Growth and Spectroscopic Properties of Er3./Yb3.-codoped Sr3.2.BO3.4 Crystal

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Growth and Spectroscopic Properties of Er3+/Yb3+-codoped Sr3...

中国化学会第五届全国结构化学学术会议

作者： Mingjun Song~(a,b) Zhoubin Lin~a Guofu Wang~(a*) a(Fujian Institute of Research on the Structure of Matter,The chinese academy of {2.,Fuzhou,Fujian 3.0002.China) b({3. School of the chinese academy of {2.,Beijing 10003.,China)

Single crystal of Yb/Er:SrY(BO) with great quality has been grown by Czochralski *** absorption and emission spectra were *** absorption band at 980 nm has an absorption coefficient of 5.15 cmand FWHM of 8.5 *** on Judd-Ofelt theory,the spectral parameters were *** intensity parameters Ω are:Ω=4.43.×10cm,Ω= 1.92.10cm,Ω=1.799×*** radiative probabilities Ar,the radiative lifetime τ,the fluorescence branching ratios β,and emission cross-sections were also *** emission cross sections σat 153.nm were calculated to be 6.96×10cm.

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压电直线电机驱动的精密工件台研制及应用

振动．测试与诊断 2013年第S2期33卷 52-54,218页

作者：刘俊标殷伯华文良栋方光荣韩立中国科学院电工研究所北京100190 中国科学院大学北京100049

为了满足在毫米量级范围内以低成本的电子束直写技术制作纳米器件和微纳米结构的需求,提出了一种基于压电陶瓷直线电机驱动的高精度二维精密工件台设计结构。该精密工件台采用精密交叉滚柱导轨,共有3.结构,采用2.nm测量分辨率的光栅尺... 详细信息

为了满足在毫米量级范围内以低成本的电子束直写技术制作纳米器件和微纳米结构的需求,提出了一种基于压电陶瓷直线电机驱动的高精度二维精密工件台设计结构。该精密工件台采用精密交叉滚柱导轨,共有3.结构,采用2.nm测量分辨率的光栅尺建立了测量系统,具有结构简单、步进精度高、定位速度快、能够在真空中运行且不产生干扰磁场等优点。利用该精密工件台在普通扫描电镜平台上构建了科研型的小型电子束曝光系统。实验结果表明,该精密工件台在某型号SEM中的拼接精度为2.nm(x方向)和63.m(y方向),并且制作了一些纳米点阵和纳米电极等结构。

关键词：电子束直写技术压电直线电机精密工件台光栅测量

Science and Technology Is Not Simply Equal to Sci-Tech

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Genomics, Proteomics & Bioinformatics 2003年第2期1卷 87-89页

作者： Xingmin Ligraduate School, chinese {2. of {3., Beijing 10003., ChinaXin Zhang (Beijing Language and Culture university, Beijing 100083. China) and Wei Gong (Beijing Genomics Institute, Beijing 1013.0, China) graduate School Chinese Academy of Sciences Beijing 100039 China

The terms "science" (Hanyu Pinyin: Kexue) and "technology" (Jishu), in western languages, are usually used separately with different meanings or together as "science and technology". However, they are often mentioned as "Kexue Jishu", or even abbreviated as "sci-tech (Keji)" in China (It is used in a similar way in Japan). It not only causes great confusion in conceptual content, but also very misleading

关键词： that on as it Science and Technology Is Not Simply Equal to Sci-Tech for been into of

Sequence analysis and expression of the Calcium-dependent protein kinase gene,MCDPK,in mulberry （Morus L.）

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Sequence analysis and expression of the Calcium-dependent pr...

现代分子植物育种与粮食安全研讨会

作者： Zhang Yinghua1,2.Lin Qiang 3.Liu Zhaoyue1,2.Tong Wei1,2.Fang Rongjun1,2.Liu Li1,2.Zhao Weiguo 1,2.* (1 College of biological and Environmental Engineering,Jiangsu university of Science and technology,Zhenjiang Jiangsu 2.2.18,China 2.Sericultural Research Institute,{2. {2. of Agricultural {3.,Zhenjiang Jiangsu 2.2.18,China 3.The General Station of Sericulture Technology Extension of Guangxi Zhuang Automomous Region,Nanning 53.007,China)

A full-length cDNA sequence coding calcium-dependent protein kinase （CDPK） of mulberry tree,which we designated MCDPK,was cloned based on mulberry expressed sequence tags （ESTs）.Sequence analysis showed that the MCDPK is 2.49 base pairs in length,encoding 53. amino acids with a predicted molecular weight of 59.96KD and an isoelectric point of *** expression level of MCDPK at different developmental stages in mulberry leaves and flowers and in different tissues was *** results showed that MCDPK transcripts were most abundantly expressed in the terminal bud,and the expression level of the mRNA could be reduced significantly under the conditions of low temperature and salt stress respectively compared to the normal growth *** research will help to understand the resistance mechanism of functional genes in mulberry in the future.

关键词： Mulberry Calcium-dependent protein kinase gene (CDPK) Gene cloning Gene expression

澜沧江上游山地典型区不同利用方式的土壤肥力性状

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山地学报 2004年第1期22卷 87-91页

作者：田昆莫剑锋陆梅常凤来杨永兴中国科学院东北地理与农业生态研究所西南林学院环境科学与工程系同济大学环境科学与工程学院吉林长春　13.012 云南昆明　6502.4 上海　2.0092

选取澜沧江上游山地8种典型土壤利用方式对土壤性质变化进行对比研究,结果表明:土壤有机质、全氮、有效氮、速效磷、速效钾、铵态氮、硝态氮之间存在显著差异,但垂直空间变异上差异不大。天然阔叶林破坏后,无论是次生林还是人工种植的... 详细信息

选取澜沧江上游山地8种典型土壤利用方式对土壤性质变化进行对比研究,结果表明:土壤有机质、全氮、有效氮、速效磷、速效钾、铵态氮、硝态氮之间存在显著差异,但垂直空间变异上差异不大。天然阔叶林破坏后,无论是次生林还是人工种植的经济林或是坡耕地和荒地,土壤养分均呈现不同程度的衰减退化。综合土壤7项指标计算定量反映土壤退化和改善程度的退化指数,表明针叶林、坡耕地、禾草荒地及桉树林退化最为严重。

关键词：土壤利用方式澜沧江上游土壤性质养分衰减退化退化指数

Probing Polymer Gate Dielectric Surface via Organic Semiconductor Transistor Performance

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Probing Polymer Gate Dielectric Surface via Organic Semicond...

全国第八届有机固体电子过程暨华人有机光电功能材料学术讨论会

作者： Wei Shao~(a,c),Zhigang Wang~b,Wenping Hu~(*a) (a Beijing National Laboratory for Molecular sciences,Key Laboratory of Organic Solids,Institute of Chemistry, chinese {2. of sciences,Beijing 100190) (b university of Science and Technology of China,Hefei 2.5}02.) (c graduate School of chinese {2. of sciences,Beijing 100190)

Organic semiconductor-based thin-film transistors have been extensively studied for organic *** search for high dielectric constant polymeric gate dielectric materials for organic field-effect transistors(OFETs) has stimulated important researched activities in both conventional and unconventional electronics due to their potential applications in large area,low cost and *** interface states,including both the dielectric surface

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