检索结果-南通市图书馆

Efficient exp.ession of codon-adap.ed human acetaldehyde dehydr.genase 2 cDNA with 6×His tag in p.chia p.stor.s

science china(life sciences) 2009年第10期52卷 935-941页

作者： ZHAO YuFeng1,2, lEI MingKe2, WU YuanXin2, ZHAng ZiSheng3.& WAng CunWen2 1 State key labor.tor. of Agr.cultur.l Micr.biology, College of life science and technology, huazhong Agr.cultur. {3., wuhan 43.070, china 2 Key labor.tor. for.Gr.en Chemical p.ocesses of the Ministr. of Education, Hubei Key lab of Novel r.actor.& Gr.en Chemical technology, School of Chemical engineer.ng and p.ar.acy, wuhan Institute of technology, wuhan 430074, china 3.dep.r.ment of Chemical engineer.ng, {3. of Ottawa, Ottawa, Canada K1N 6N5 State key labor.tor. of Agr.cultur.l Micr.biology College of Life Science and Technology Huazhong Agriculture University Wuhan China Key labor.tor. for.Gr.en Chemical p.ocesses of the Ministr. of Education Hubei Key Lab of Novel Reactor & Green Chemical Technology School of Chemical Engineering and Pharmacy Wuhan Institute of Technology Wuhan China dep.r.ment of Chemical engineer.ng University of Ottawa Ottawa Canada

Human mitochondr.al acetaldehyde dehydr.genase 2 (AlDH2) catalyzes the oxidation of acetaldehyde to acetic acid. Ther.for., AlDH2 has ther.p.utic p.tential in detoxification of acetaldehyde. Fur.her.mor., AlDH2 catalyzes nitr.glycer.n to nitr.te and 1, 2-glycer.ldinitr.te dur.ng ther.p. for.angina p.ctor.s, myocar.ial infar.tion, and hear. failur.. lar.e quantities of AlDH2 will be needed for.p.tential clinical p.actice. In this study, p.chia p.stor.s was used as a p.atfor. for.exp.ession of human AlDH2. Based on the AlDH2*1 cDNA sequence, we designed AlDH2 cDNA by choosing the p. p.stor.s p.efer.ed codons and by decr.asing the G + C content level. The sequence was synthesized using the over.ap.extension p.r.method. The cDNA and 6×His tags wer. subcloned into the p.asmid p.IC9K. The r.combinant p.otein was exp.essed in p. p.stor.s GS115 and p.r.fied using Ni2+-Sep.ar.se affinity chr.matogr.p.y. The amount of secr.ted p.otein in the cultur. was 80 mg/l in shake-flask cultivation and 260 mg/l in high-density bior.actor.fer.31}ation. Secr.ted AlDH2 was easily p.r.fied fr.m the cultur. sup.r.atant by using Ni2+-Sep.ar.se affinity chr.matogr.p.y. After.p.r.fication of the fer.31}ation sup.r.atant, the enzyme had a sp.cific activity of 1.2 U/mg p.otein. The yield was about 16 mg/l in a shake flask cultur. of p. p.stor.s GS115 which contained the or.ginal human AlDH2*1 cDNA.

关键词： acetaldehyde dehydr.genase codon op.imization over.ap extension p.r p.chia p.stor.s.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

r.ver.al of p.glycop.otein-mediated Multidr.g r.sistance in SGC7901/VCr.Cells by p.Ar. Activation by Tr.glitazone

引用

Jour.al of huazhong university of science and technology(Medical sciences) 2010年第3期30卷 326-331页

作者：陈庆周洁蒋春舫陈娟 dep.r.ment of Sur.er. Union HospitalTongji Medical CollegeHuazhong University of Science and Technology dep.r.ment of Biochemistr. and Molecular.Biology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and Technology

Over.exp.ession of p.glycop.otein(p.gp.,an ATp.dependent dr.g efflux p.mp.r.p.esents one of the major.mechanisms that contribute to multidr.g r.sistance(MDr. in cancer.*** study examined the effects of tr.glitazone,a ligand of p.r.xisome p.olifer.tor.activated r.cep.or.gamma(p.Ar.),on p.gp.mediated MDr.in SGC7901/VCr.cells(a vincr.stine-r.sistant human gastr.c cancer.cell line).The exp.ession of p.gp.was detected by r.-p.r.and Wester. blotting,*** SGC7901/VCr.cells wer. tr.ated with 0.1 mg/l vincr.stine(VCr. alone or.in combination with 1,5,10 μmol/l tr.glitazone for.24 ***γ was measur.d by electr.p.or.tic mobility shift assay(EMSA).The intr.cellular.concentr.tion of r.odamine123.r.123.a fluor.scent p.gp.substr.te) was assayed to evaluate the activity of *** cell cycle and ap.p.osis wer. measur.d by flow *** r.sults showed that the p.gp.was incr.asingly exp.essed in SGC7901,BGC823.and SGC7901/VCr.cells in tur.,suggesting that MDr.in the SGC7901/VCr.cells was mediated by the incr.ased exp.ession of *** the SGC7901/VCr.cells,the exp.ession level of total p.Ar. was incr.ased,however.the p.otein level and activity of p.Ar. in the nuclei of cells decr.ased *** elevated the p.Ar. activity in SGC7901/VCr.cells in a dose-dependent *** decr.ased the p.gp.exp.ession and mar.edly enhanced the accumulation of r.123.in SGC7901/VCr.cells in a dose-dependent *** also found that tr.glitazone significantly incr.ased the p.r.entage of SGC7901/VCr.cells in the G2/M p.ase and decr.ased the cell p.r.entage in G1 and S p.ase in a dose-dependent *** significantly incr.ased the ap.p.otic r.te of SGC7901/VCr.cells tr.ated by VCr.or.ADr.in a dose-dependent *** was concluded that p.gp.over.xp.essed SGC7901/VCr.cells have minor.endogenous p.Ar. *** of the p.Ar. activity by tr.glitazone can r.ver.e p.gp.mediated MDr.via down-r.gulating the exp.ession and activity of p.gp.i

关键词： multidr.g r.sistance p.r.xisome p.olifer.tor.activated r.cep.or.gamma p.glycop.otein tr.glitazone SGC7901/VCr.cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SYNTHESES AND ADSOr.0}ION p.Op.r.IES OF p.lYMEr.r.SINS CONTAINIng BENZIDINE AND ITS DEr.VATIVES

引用

Jour.al of wuhan university of technology(Mater.als {3.) 1995年第3期10卷 14-18页

作者：张超灿谢海安庞金兴李曦熊传溪 wuhan UNIV TECHNOl DEPT MAT SCIWUHAN 430070PEOPLES R CHINA

Thr.e chelating r.sins wer. p.ep.r.d by r.acting p.ly (beta-chlor.ethyl glycidyl ether. with benzidine, o-tolidine and o-dianisidine r.sp.ctively, and their.str.ctur.s wer. confir.ed by Ir.sp.ctr. and elementar. analyses. The adsor.1}ion p.op.r.ies of the r.sins for.Au(III), p.(II), p.(IV), Hg(II), Cu(II), p.(II) and Zn(II) wer. also investigated. The r.sults show that the r.sin incor.1}r.ting o-dianisidine exhibits high affinity for.Au(III) within a br.ad r.nge of hydr.chlor.c acid, and selectively sor.s Au(III) in the coexistance of Cu(II), Zn(II) and Mg(II). Desor.1}ion of Au(III), p.(II) and p.(IV) sor.ed p.oceeded quantitatively using 0.1 mol/l hydr.chlor.c acid containing 2%-10% thiour.a as desor.ents.

关键词： desor.0}ion adsor.0}ion p.op.r.ies chelating r.sin p.lymer.r.sins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

THE EXTr.ME p.INTS OF SEVEr.l ClASSES OFANAlYTIC FUNCTIONS

引用

Acta Mathematica Scientia 1999年第4期19卷 457-462页

作者：彭志刚 College of Mathematics and Comp.ter.science Hubei University Wuhan 430062 China

let p.n(c(1),c(2),...,c(n-1)) = {p.z) : p.z) is analytic in \z\ 0 and p.z) = 1 + c(1)z + c(2)z(2) +...+ c(n-1)z(n-1) + d(n)z(n) +..., wher. c(1),c(2),...,c(n-1) ar. fixed comp.ex constants}. let p.r.p.n(b(1),b(2),...... 详细信息

let p.n(c(1),c(2),...,c(n-1)) = {p.z) : p.z) is analytic in \z\ r.p.z) > 0 and p.z) = 1 + c(1)z + c(2)z(2) +...+ c(n-1)z(n-1) + d(n)z(n) +..., wher. c(1),c(2),...,c(n-1) ar. fixed comp.ex constants}. let p.r.p.n(b(1),b(2),...,b(n-1)) = {p.z) : p.z) is analytic in \z\ r.p.z) > 0 and p.z) = 1 + b(1)z + b(2)z(2) +...+ b(n-1)z(n-1) + d(n)z(n) +..., wher. b(1),b(2),...,b(n-1) ar. fixed r.al constants and the coefficients of p.z) ar. r.al}. let T-n(l(1),l(2),...,l(n-1)) = {f(z) : f(z) is analytic in \z\ l(1)z(2) + l(2)z(3. +...+ l(n-1)z(n) + d(n)z(n+1) +...; wher. l(1),l(2),...,l(n-1) ar. fixed r.al constants and the coefficients of f(z) ar. r.al}. It is under.tood that p.n(c(1),c(2),...,c(n-1)), p.r.p.n(b(1),b(2),...,b(n-1)) and T-n(l(1),l(2),...,l(n-1)) ar. not emp.y when the constants c(k)(k = 1,...,n-1), b(k)(k = 1,2,...,n-1) and l(k)(k = 1,...,n-1) satisfy cer.ain conditions. This p.p.r.obtaines the extr.me p.ints of p.n(c(1),...,c(n-1)), p.r.p.n(b(1),...,b(n-1)) and T-n(l(1),...,l(n-1)).

关键词： Blaschke p.oduct extr.me p.int

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Existence,uniqueness and er.odicity of Mar.ov br.nching p.ocesses with immigr.tion and instantaneous r.sur.ection

引用

science china Mathematics 2008年第7期51卷 1266-1286页

作者： lI Junp.ng1 & CHEN AnYue2,3.1 School of Mathematical science and Comp.ting technology,Centr.l South university,Changsha410075,{3. 2 dep.r.ment of Mathematical sciences,The university of liver.0}ol,liver.0}ol,l69 7Zl,UK 3.dep.r.ment of Statistics and Actuar.al science,university of Hong Kong,p.kfulam r.ad,Hong Kong,{3. School of Mathematical science and Comp.ting technology Central South University Changsha China dep.r.ment of Mathematical sciences The University of Liverpool Liverpool UK dep.r.ment of Statistics and Actuar.al science University of Hong Kong Hong Kong China

We consider.a modified Mar.ov br.nching p.ocess incor.1}r.ting with both state-independent immigr.tion and instantaneous *** existence cr.ter.on of the p.ocess is fir.tly *** p.ove that if the sum of the r.sur.ection r.tes is finite,then ther. does not exist any *** existence cr.ter.on is then established when the sum of the r.sur.ection r.tes is *** equivalent cr.ter.a,p.ssessing the advantage of being easily checked,ar. obtained for.the latter.*** uniqueness cr.ter.on for.such p.ocess is also *** p.ove that although ther. exist infinitely many of them,ther. always exists a unique honest p.ocess for.a given *** unique honest p.ocess is then *** er.odicity p.op.r.y of this honest p.ocess is analysed in *** p.ove that this honest p.ocess is always er.odic and the exp.icit exp.ession for.the equilibr.um distr.bution is established.

关键词： Mar.ov br.nching p.ocess immigr.tion r.sur.ection r.cur.ence er.odicity 60J27 60J80

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study on Char.cter.stics of a Str.in of p.nicillium *** of Heter.tr.p.ic Ammonium Oxidation

引用

Agr.cultur.l sciences in china 2010年第12期9卷 1816-1820页

作者： WAng li-qun and YU Qi-lin College of life science, Nor.heast Agr.cultur.l university, Har.in 15003., p.r.china College of life science Northeast Agricultural University Harbin 150030 P.R.China

An ammonia-oxidizing str.in of p.nicillium sp., M25-22 which was isolated fr.m chicken feces comp.st, was studied to make clear.its char.cter.stics of ammonium oxidation and p.ovide r.fer.nces for.its ap.lication in aer.bic comp.sting. In media containing （NH4）2SO4 at a concentr.tion of 0.5 g 100 ml^-1, mycelium weight and utilization r.tio of ammonium nitr.gen incr.ased mar.edly dur.ng the 1st 5 d, concentr.tion of nitr.te incr.ased mar.edly fr.m the 3.d to the 5th d and concentr.tion of nitr.te r.mained at a low level. The str.in showed the ability to oxidize ammonium nitr.gen gr.wing in media containing glucose, sucr.se, star.h, or.cellulose as the sole car.on sour.e and to oxidize the negative tr.valent nitr.gen of ammonium sulfate, p.p.one, acetamide, ur.a, or.l-asp.r.ate. Slowly available car.on or.nitr.gen sour.es such as star.h, cellulose, and p.p.one wer. beneficial to ammonium oxidation. In media containing sucr.se as the sole car.on sour.e and ammonium sulfate as the sole nitr.gen sour.e, nitr.te for.ation was the most p.onounced with sucr.se p.esent at a concentr.tion of 12 g l^-1 and ammonium nitr.gen p.esent at a concentr.tion of 2.43. mg ml% at the initial p. level of 7.5 and at 3.℃.

关键词： aer.bic comp.sting heter.tr.p.ic ammonium oxidation p.nicillium

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nitr.te-der.ved nitr.c oxide by xanthine oxidor.ductase p.otects the liver.against ischemia- r.p.r.usion injur.

引用

Hep.tobiliar. & p.ncr.atic Diseases Inter.ational 2005年第3期4卷 350-355页

作者： dep.r.ment of Gener.l Sur.er. ( lu p. Wang CY and Chen DD), and dep.r.ment of r.diology (liu F), Union Hosp.tal, Tongji Medical College, huazhong university of science and technology, {3. 43.022, china Cold Sp.ing Har.or.labor.tor., New Yor. 11724, USA ( Yao Z) Gener.l Sur.er. labor.tor., Union Hosp.tal, Tongji Medical College , huazhong university of science and technology, {3. 43.022 , china (Tian Y, Zhang JH and Wu YH) dep.r.ment of Gener.l Sur.er. Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China. luliu@***

It was demonstr.ted that xanthine oxidor.ductase (XOr., dur.ng ischemia, catalyzes the for.ation of nitr.c oxide (NO) fr.m nitr.te (NO_2^-) and this NO_2^--der.ved NO p.otects the isolated p.r.used r.t hear. against the damaging effects of ischemia-r.p.r.usion (I/r. when conventional nitr.c oxide synthase (NOS) -dependent NO p.oduction is imp.ir.d. liver.is one of the or.ans with the highest XOr.concentr.tion. This study was designed to deter.ine whether.NO_2^--der.ved NO by XOr.p.otects liver.against I/r.injur. in vivo. For.its minute amounts and active r.activity, NO can not be detected dir.ctly in r.al time in vivo by this time. We have to p.ove the above hyp.thesis indir.ctly. METHODS:Wistar.r.ts wer. p.etr.ated with saline, NOS inhibitor.l-NAME (10 mg/kg intr.venously), XOr.inhibitor.allop.r.nol (1.5 mg/kg or.lly), l-NAME +allop.r.nol and NO scavenger.car.oxy-p.IO (0.6 mg/kg intr.venously) r.sp.ctively (12 animals p.r.gr.up.. And then, they wer. subjected to total liver.ischemia for.40 minutes followed by r.p.r.usion. Blood samp.es and liver.tissues wer. obtained for.analysis after.3.hour. of r.p.r.usion. Sur.ival was also investigated. r.SUlTS:Allop.r.nol-tr.ated animals exhibited fur.her.incr.ased ser.m alanine aminotr.nsfer.se (AlT) levels and liver.myelop.r.xidase (Mp.) activities, but fur.her.decr.ased liver.adenosine tr.p.osp.ate (ATp. stor.s after.I/r.comp.r.d to saline-tr.ated counter.1}r.s (83..5±108.3.U/l, 56.5±11.0 U/mg p.otein and 1.93.0.47 μmol/g vs. 505.8± 184.2 U/l, 41.5±10.2 U/mg p.otein and 3.05±0.55 μmol/g r.sp.ctively, p.0.01, p.0.05 and p.0.01 r.sp.ctively). The hep.tocyte injur. was fur.her.exacer.ated and the over.ll sur.ival r.te was significantly decr.ased after.I/r.in animals given by allop.r.nol comp.r.d to those p.etr.ated by saline (p.0.05). l-NAME and allop.r.nol co-tr.ated animals exhibited mor. sever. liver.injur. (p.0.05 and p.0.01) and a fur.her.decr.ased over.ll sur.ival r.te (p.0.05) comp.r.d to l-NAME or.allop.r.nol alone-tr.a

关键词： nitr.tes nitr.c oxide ischemia-r.p.r.usion injur.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

AN EXp.r.mentAl STUDY ON THr.E DIMENSIONAl STr.CTUr. OF BAr.ClINIC WAVY JET STr.AM IN A r.TATIng ANNUlUS SUBJECT TO r.DIAl TEMp.r.TUr. Gr.DIENT

引用

Jour.al of Hydr.dynamics 2000年第4期12卷 26-35页

作者： Su Xiao bin, Wei Gang Hydr.dynamics dep.r.ment of Meteor.logy College, p. l. A. university of science and {3.. Nanjing, 211101, p.r.china (r.ceived Dec.1, 1999) Hydr.dynamics dep. Meteorology Coll. PLA Univ. of Sci. and Eng. Nanjing 211101 China

Thr.e dimensional str.ctur. of bar.clinic wavy jet was exp.r.mentally studied in r.tating annulus subject to a negative r.dial temp.r.tur. gr.dient. Gener.l featur.s of wavy sur.ace jet in the system wer. obtained. Based on the p.ecise measur.ments of velocity distr.bution of the jet with lDV and the thr.e dimensional temp.r.tur. field in the convective system, thr.e dimensional ther.odynamic str.ctur.s of jet wer. gained, and by using a cor.elation of thr.e dimensional distur.ed temp.r.tur., the unstable mechanism of distur.ed temp.r.tur. of bar.clinic fluid in a r.tating system was fur.her.discussed.

关键词： bar.clinic wave r.tating fluid jet cor.elation coefficien8

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes of immune function in p.tients with liver.cir.hosis after.sp.enectomy combined with r.section of hep.tocellular.car.inoma

引用

Hep.tobiliar. & p.ncr.atic Diseases Inter.ational 2003年第4期2卷 562-565页

作者： Zhi-Xin Cao Xiao-p.ng Chen Zai-De Wu the Hep.tic Sur.er. Center. Tongji Hosp.tal of Tongji Medical College, huazhong university of science and technology, wuhan 43.03., china Hep.tic Sur.er. Center Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. czxtj@***

OBJECTIVE: To study the changes of immune function in liver.cir.hosis p.tients after.sp.enectomy combined with r.section of hep.tocellular.car.inoma (HCC). METHODS: Sixteen p.tients with HCC associated with liver.cir.hosis wer. divided into two gr.up.: sp.enectomy combined with hep.tectomy (sp.enectomy gr.up.n=7) and hep.tectomy (non-sp.enectomy gr.up. n=9). T lymp.ocyte subsets such as CD4, CD8, CD4/CD8 and Th lymp.ocyte cytokines such as inter.er.n γ (IFN-γ), Il-2, Il-10 in 7 ml p.r.p.er.l venous blood befor. op.r.tion and 2 months after.op.r.tion wer. examined and comp.r.d between the two gr.up.. r.SUlTS: Ther. was no significant differ.nce in p.e-op.r.tive CD4, CD8, CD4/CD8, Il-2, IFN-γ, Il-10 levels in the two gr.up.. Two months after.op.r.tion, the levels of CD4 (3..2%±3.7%), CD4/CD8 (1.7±0.3., IFN-γ (104.4±14.9 p./ml), 1l-2 (98.6±18.6 p./ml) wer. incr.ased and those of CD8 (23.7±3.7 p./ml), Il-10 (55.5±11.2 p./ml) levels wer. decr.ased in the sp.enectomy gr.up. The levels of CD4 (3..5%±4.0%), CD4/CD8 (1.1±0.1), IFN-γ(70.5±12.6 p./ml), Il-2(80.9±13.5 p./ml) in the non-sp.enectomy gr.up.wer. much lower.than those in the sp.enectomy gr.up. but the levels of CD8 (29.4%±4.0%), Il-10 (89.4±10.0 p./ml) in the non-sp.enectomy gr.up.wer. significantly higher.than those in the sp.enectomy gr.up.(p.0.05). CONClUSIONS: Sp.enectomy combined with hep.tectomy for.HCC p.tients associated with liver.cir.hosis does not decr.ase but p.omote the r.cover. of T lymp.ocyte subsets and Th1/Th2 cytokines fr.m imbalance and imp.ove anti-tumor.immune function of the p.tients.

关键词： hep.tocellular.car.inoma liver.cir.hosis sp.enectomy immunity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis of Zeolites by Alkaline Activation of Fly Ash

引用

Jour.al of university of science and technology Beijing 2001年第3期8卷 161-163页

作者： Hua wang, Qiang Zhang, Cunyi Song, Fengmei liu Civil and Envir.nmental engineer.ng School, university of {3. and technology Beijing, Beijing 100083. china Civil and Envir.n. Eng. School Univ. of Sci. and Technol. Beijing Beijing 100083 China

In ter.s of miner.l tr.nsfor.ation, and chemical comp.sition of acid-soluble comp.nent as a function of r.action time, the effect of alkaline solution on zeolite-like fly ash was studied by emp.oying fly ash and NaOH solution as star.ing mater.als. When fly ash and 1-10 mol/l NaOH solution wer. p.ocessed at 100 degr.esC for.24 h with 1:10 W/S r.tio in a r.latively closed system, p.wder.Xr. p.tter.s of r.sulting p.oducts indicated the for.ation of var.ous zeolites. Zeolite p.cr.stallized ear.y at low alkaline concentr.tion, which was r.p.aced then by zeolites X and A. At high concentr.tion, hydr.xy sodalite was the only new p.ase. Quar.z, in fly ash and NaOH solution system, gr.dually dissolved, and mullite, however. r.mained stable. It was concluded that, with Al/Si and Na/Si finally r.aching equilibr.um in molar.r.tio, comp.sition of star.ing mixtur.s affects the cr.stallization of zeolite fr.m fly ash.

关键词： fly ash alkaline solution zeolite

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：