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Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

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chemical Research in Chinese Universities 2012年第1期28卷 103-107页

作者： ZHU Chun-yu1,2, SUN Ting-ting2, ZHAO Jian1,2, WANG Ning1,2, ZHENG Fang-liang1, AI Hai-xin1, ZHU Jun-feng1,2, WANG Xiao-ying3. ZHU Ying4, WU Jian-guo4 and LIU Hong-sheng1,2 1. Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province, 2. Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province, Academy of Science, Liaoning university, shenyang 11003., P. R. china 3. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, P. R. china 4. State Key Laboratory of Virology, Wuhan university, Wuhan 43.072, P. R. china Liaoning Univ Acad Sci Key Lab Anim Resource & Epidem Dis Prevent Liaoni Shenyang 110036 Peoples R China Liaoning Univ Acad Sci Res Ctr Comp Simulating & Informat Proc Biomacrom Shenyang 110036 Peoples R China Chinese Ctr Dis Control & Prevent Inst Nutr & Food Safety Beijing 100050 Peoples R China Wuhan Univ State Key Lab Virol Wuhan 430072 Peoples R China

Avian influenza virus（AIV） nonstructural 1（NS1） gene was amplified by real-time polymerse chain reac tion（RT-PCR） and inserted into pET28a, then transformed into E. coli BL21（DE3. competent cell. With the induction of isopropyl-β-D-thiogalactoside（IPTG） and the purification of Ni-NTA column, we finally obtained purified NS1 protein. T7-phage display system was used to screen the proteins that interacted with NS1 from lung cell cDNA li brary. The selected positive clones were identified by DNA sequencing and analyzed by BLAST program in Gene Bank. Two proteins were obtained as NS1 binding proteins, Homo sapiens nucleolar and coiled-body phosphoprotein 1（NOLC1） and Homo sapiens similar to colon cancer-associated antigen. By co-immunoprecipitation and other me thods, Homo sapiens NOLC1 was found to interact with the NS1 protein, the results would provide the basis for fur ther studying biological function of NS1 protein.

关键词： T7-phage display Nonstructural 1 (NS 1) protein Interacting protein

Comparative Analysis of Photosynthesis of Three Stipa Species Based on A Photosynthesis Model

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Comparative Analysis of Photosynthesis of Three Stipa Specie...

Morphological and histological studies on the telencephalon of the salamander Onychodactylus fischeri at different stages after embryo

2011 Chinese Control and Decision Conference（CCDC）

作者： Chan Zhou is with College of Life Science,Liaoning university,shenyang,11003.,{3. Zhuo Zhang is with College of Biology and Environment Engineering,shenyang university,shenyang,110044,{3. .Yunfei Yang is with Institute of Grassland Science,Northeast normal university,Key Laboratory for Vegetation Ecology,Ministry of Education,P.R.{3.,Changchun,13.024,{3..Qiong Gao is with College of Resources Science and Technology,Beijing normal university,Key laboratory of Environmental Change and Natural Disasters,Ministry of Education,Beijing,100875,{3..

We compared photosynthetic characteristics of three Stipa species in NECT,Stipa baicalensis,*** and *** based on ecophysiological traits derived from parameters of a photosynthesis *** net photosynthesis rates,transpiration rate and stomatal conductance,together with microclimate variables of the respective species and sites were observed in July *** photosynthesis rates of three Stipa species were then regressed on incident light intensity,leaf temperature and CO2 partial pressure,and stomatal conductance by means of the nonlinear least square technique and the modified Thornley and Johson’s leaf photosynthesis *** results showed that Thornley and Johson’s photosynthesis model explained on 60.49%,86.17% and 81.3.% of variations in observed net photosynthesis rates for three ***,*** and ***,*** difference in photosynthetic characteristics for three Stipa species explained adaptation to conditions of their respective *** photo efficiency of *** is an adaption to lower photosynthetic active radiation （PAR） at high latitude of the meadow steppe *** the other hand,*** in typical steppe and *** in desert steppe sites are located at lower latitude with more available PAR,thus they showed relative lower photon *** conductance of *** is low because of their low leaf nitrogen concentration,which may be caused by the low available soil nitrogen due to low decomposition rates at high *** contrast to ***,*** shows much lower base rate of mitochondria respiration because of higher leaf temperature often experienced by plants at desert steppes.

关键词： Stipa species photosynthesis characteristics photosynthesis model

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Morphological and histological studies on the telencephalon ...

PTEN encoding product:a marker for tumorigenesis and progression of gastric carcinoma

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ying-Ying ZHOU~1,Huan-Huan WANG~1,Li-Jie GUO~1,Ran SHAO~1,Li-Wen WANG~(1,*) 1 College of Chemistry and Life Science,shenyang normal university,shenyang 11003. china

＜正＞Objective To dissect the developmental process of never system in amphibian and identify the classified status of salamander Onychodactylus fischeri in the *** The morphological and histological investigation on telencephalon of salamander Onychodactylus fischeri at different developmental stages were conducted using paraffin section technique and HE-staining *** The morphological investigation showed that the telencephalon of Onychodactylus fischeri in each age group is composed of a hemisphere and an olfactory bulb,and the latter locates at the ventrolateral portion of the rostral *** line between the olfactory bulb and the hemisphere is obscure in dorsal view of the *** GroupⅠtoⅣ,the proportion of telencephalon length to brain length increased gradually, whereas that of brain length to body length exhibited a decreasing *** histology,lamination of the olfactory bulb in larva was not obvious,but in subadult and adult individuals,the multi-layered structure was distinct and six layers can be identified from the lateral portion to the medial portion,including stratum nervosum,glomerular layer,external plexiform layer,mitral cell layer,internal plexiform layer and granule cell *** could be easily distinguished from cells in primordial piriform *** general pallium which locates at the most dorsal extent of the ventricle,is difficult to distinguish from the dorsal primordial hippocampus medially and from the primordial piriform *** in the primordial general pallium equably distribute in GroupⅠ,ⅡandⅢ,that is,cells take up almost the whole region in the dorsal part of the *** in the telencephalon of GroupⅣ,ⅤandⅥ,neurons of the primordial general pallium distribute in a confined area of the dorsal *** far as the thickness of area where cells distribute in the primordial general pallium,GroupⅠis similar with GroupⅡ,which is more than 100 ***Ⅲis 162μm

关键词： salamander development telencephalon morphology histology

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World Journal of Gastroenterology 2003年第1期9卷 35-39页

作者： Lin Yang Li-Ge Kuang Hua-Chuan Zheng Jin-Yi Li Dong-Ying Wu Su-Min Zhang Yan Xin No.4 Lab,Cancer Institute,The First Affiliated Hospital,china Medical university,shenyang 110001,china Ying Chen Shen Yang Gynecology & Obstetrics Hospital,shenyang 110014,china No.4 Lab Cancer Institute The First Affiliated Hospital China Medical University Shenyang 110001 China. yxin@***

AIM: To detect the expression of PTEN encoding productin normal mucosa, intestinal metaplasia (IM), dysplasia andcarcinoma of the stomach, and to investigate its clinicalimplication in tumorigenesis and progression of ***: Formalin-fixed paraffin embedded specimens from184 cases of gastric carcinoma, their adjacent normal mucosa,IM and dysplasia were evaluated for PTEN protein expressionby SABC immunohistochemistry. PTEN expression wascompared with tumor stage, lymph node metastasis, Lauren'sand WHO's histological classification of gastric *** of VEGF was also detected in 60 cases of gastriccarcinoma and its correlation with PTEN was ***: The positive rates of PTEN protein were 100 %(102/102), 98.5 %(65/66), 66.7 % (4/6) and 47.8 %(88/184)in normal mucosa, IM, dysplasia and carcinoma of the stomach,respectively. The positive rates in dysplasia and carcinomawere lower than in normal mucosa and IM (P＜0.01).Advanced gastric cancers expressed less frequent PTEN thanearly gastric cancer (42.9 % v567.6 %, P＜0.01). The positiverate of PTEN protein was lower in gastric cancer with thanwithout lymph node metastasis (40.3.% v563.3.%, P＜0.01).PTEN was less expressed in diffuse-type than in intestinal-type gastric cancer (41.5 % v557.8 %,P＜0.05). Signet ringcell carcinoma showed the expression of PTEN at the lowestlevel (25.0 %, 7/28); less than well and moderatelydifferentiated ones (P＜0.01). Expression of PTEN was notcorrelated with expression of VEGF (P＞0.05).CONCLUSION: Loss or reduced expression of PTEN proteinoccures commonly in tumorigenesis and progression of gastriccarcinoma. It is suggested that PTEN can be an objective markerfor pathologically biological behaviors of gastric carcinoma.

关键词： PTEN 肠化生胃癌肿瘤发生肿瘤病理学细胞因子免疫组织化学

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独立市场和平稳市场中金融原则的几个性质

沈阳化工学院学报 2004年第1期18卷 58-61页

作者：孙丽沈阳师范大学高等职业技术学院辽宁沈阳110036

　研究在金融市场中无风险控制下,投资者连续投资,财富不断积累,即在一个周期的投资结束后连本带利全部或部分的再投资,换言之,投资者不再增加也不减少资金的情况下的收益情况.引入Ln 函数,避开Log 函数的诸多不变,在经济分析中更加明了... 详细信息

　研究在金融市场中无风险控制下,投资者连续投资,财富不断积累,即在一个周期的投资结束后连本带利全部或部分的再投资,换言之,投资者不再增加也不减少资金的情况下的收益情况.引入Ln 函数,避开Log 函数的诸多不变,在经济分析中更加明了.分析单周期投资模型达到Ln 最优资产组合的条件,得到序列投资模型中,独立市场、平稳市场模型的Ln 最优投资组合的平均获利最大、渐进最优性质.

关键词： Ln-最优资产组合投资模型风险收益资产组合独立市场平稳市场

Factors Influencing Size of Polystyrene Microspheres in Emulsifier-free Emulsion Polymerization

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Factors Influencing Size of Polystyrene Microspheres in Emul...

Liquid Chromatography Detertmination of Amino Acids by Pre-Column Fluorescence Derivatization with Acridone-N-acetyl Chloride

第八届国际粉体检测与控制学术会议

作者： Liu Yanhui,Shao Zhongcai,Cui Xiaosong College of Material Science and Engineering, shenyang Ligong university,china No.2,Nanping Zhong Road,Hunnan New District,shenyang,110168 china

As functional polymer materials,monodispersed polystyrene microspheres with microns size could be assembly into photonic *** are of wide prospects for using in many fields such as template materials,optical filer materials, light switch,high-density electromagnetic data storage and chemical and biological chemical sensors,*** monodisperse micron-size polystyrene particles were prepared by emulsifier-free emulsion *** effects of styrene,potassium persulfate concentration and stir velocity on the average diameter of polystyrene and its distribution were mainly *** results showed that with the increasing of concentration of potassium persulfate,particle size *** the increasing of concentration of styrene and stir velocity,particle size increased firstly and then decreased. When the concentration of potassium persulfate was 1.0 mg/ml,the concentration of styrene was 0.08 g/mL and stirring velocity was 23. r/min,size of polystyrene particles were 1.4μm and standard deviation was *** of synthesized products and FT-IR contrastive analysis showed that polystyrene was prepared.

关键词： Emulsifier-free emulsion polymerization Polystyrene particle Particle size Monodispersion

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Chinese chemical Letters 1998年第7期9卷 657-660页

作者： Jin Mao YOU Xin Jun FAN Qing Yu OU Qing Cun ZHU (Lanzhou Institute of chemical Physics, Chinese Academy of Sciences 73.000)(Department of Chemistry, Qufu normal university, Qufu, Shandong 273.65) Lanzhou Inst. of chemical Physics Chinese Academy of Sciences 730000 China Department of Chemistry Qufu Normal University Qufu Shandong 273165 China

A sensitive liquid chromatographic mcthod for the detection of andno acids with precolumn fluorescence derivatization with acridone-N-acetyl chloride has been developed. Andno acid derivatives were eluted in 4o ndn with good reproducibility. The reIative standard deviations (n=6) at an analytical concentration of 50 pmol are biological samples.

关键词： Liquid chromatography, Acridone-N-acetyl Chloride Amino acids, Derivatization

HPLC Determination of Amino Acids by Pre-Column Fluorescence Derivatization with Carbazole-9-yl-Acetyl Chloride

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Chinese chemical Letters 1998年第3期9卷 281-285页

作者： Jin Mao YOU Xing Jun FAN Qing Yu OU Qing Cun ZHU(Lanzhou Institute of chemical Physics, Chinese Academy of Sciences 73.000)(Department of Chemistry, Qufu normal university, Qufu, Shandong 273.65) Chinese Acad Sci Lanzhou Inst Chem Phys Lanzhou 730000 Peoples R China Qufu normal Univ Dept Chem Shandong 273165 Peoples R China

A sensitive HPLC method for the detection of amino acids with pre-column fluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method, in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtained on a reversed-phase C-18 column. Derivatization and chromatographic conditions were optimized. Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standard deviations (n=6) at an analytical concentration of 100 pmol are biological samples.

关键词： liquid chromatography amino acids fluorescence derivatization carbazole-9-yl-acetyl chloride

Synthesis of A New Group of Flavones Containing Crown Ether Moiety

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chemical Research in Chinese Universities 1995年第2期11卷 170-173页

作者： LI Ting-sheng,ZHU Chuan-fang and LI Zhong-hua Wept,of Chem.,Central china normal university,Wuhan,43.070)WEI Xu-dong and HU Hong-wen (Department oj Chemistry,Nanjing university,Nanjing.210093. CENT china normal UNIV DEPT CHEMWUHAN 430070PEOPLES R CHINA NANJING UNIV DEPT CHEMNANJING 210093PEOPLES R CHINA

Flavones are main components of natural occurring flavonoils. It is well documented in the literatures that flavones are known to exhibit a wide variety of biological and pharmaceutical activities. Many of them have been used therapentically for the treatments of symptoms of chronic venous insufficiency, of varicose veins, of conditions and of diabetic retinopathy, etc.. Some flavones have

关键词： Flavone Crown ether Synthesis