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Formation of complexes of Rubisco-Rubisco activase from La^3., Ce^3. treatment spinach

Science china Chemistry 2005年第1期48卷 67-74页

作者： HONG Fashui1, LIU Chao1, ZHENG Lei1, WANG Xuefeng1, WU Kang1, SONG Weiping1, Lü Shipeng1, TAO Ye2.& ZHAO Guiwen3.1. College of Life sciences, Suzhou Universty, Suzhou 2.5006,{2. 2. Synchrotron Radiation Laboratory, institute of high energy physics, The {3. Academy of Science, Beijing 10003.,{2. 3. Chemical Department, University of Science and Technology of{2., Hefei 2.0}02.,{2. College of Life sciences Suzhou Universty Suzhou China Synchrotron Radiation Laboratory Institute of High Energy Physics The Chinese Academy of Science Beijing China Chemical Department University of Science and Technology of China Hefei China

The complex of Rubisco and Rubisco activase from LaCl3., CeCl3. treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3., CeCl3. treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3., CeCl3. treated spinach have not only a band of Rubisco (560 kD), but also a band of about 1100 kD, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3., CeCl3.treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 3.—3. –SH per mol enzyme, 14—2. –SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3., CeCl3.-treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3., CeCl3. treated spinach in vivo are 1.5 and 1.9 times those of the control.

关键词： La3., Ce3., spinach, Rubisco, complex of Rubisco and Rubisco acitivase, spectral characteristics.

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Study on the two and three-parameter working stability regions of 3.-TZP suspension

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Science china(Technological sciences) 2006年第5期49卷 547-568页

作者： CHEN Youqiang1, 2. ZHANG Xinni3. CHEN Sha’ou3.& QI Ping3.1. Department of Chemistry, Qingdao University, Qingdao 2.6071, china 2. Department of Chemistry, Tsinghua University, Beijing 100084, china 3. Department of physics, Qingdao University, Qingdao 2.6071, china Department of Chemistry Qingdao University Qingdao China Department of Chemistry Tsinghua University Beijing China Department of physics Qingdao University Qingdao China

We determined the variation tendency of viscosity (η) at various solids load- ing (α) for 3.-TZP suspension as a function of dispersant concentration (Cw) using vis- cosity measurement, measured the diameter of particles as a function of Cw in very dilute suspension using light scattering method, and obtained the surface features of the sedi- ment of suspension with different Cw using SEM. We also discussed the influence of the microstructure of adsorbed polymer layers on particles and their interactions in the dis- persing medium on the stability of suspension. Then two different stable states and two different unstable states for 3.-TZP suspension were given. Accordingly, the (Cw, η), (Cw, α), and (α, η) two-parameter, and (Cw, α, η) three-parameter stability maps were con- structed. Based on the DLVO theory calculations, the dispersant concentration, particle distance (r), and interparticle potential {2. (VT) maps with various solids loading for 3.-TZP aqueous suspension were also constructed. In (Cw, r) two-parameter coordinate system, the stable motion region map for particles was established. The Cw, α, and VT,max (the highest potential {2.) surface of the potential barrier map was obtained through calculation and simulation on the basis of the surfaces of Cw, r, VT maps introduced above. The (Cw, α) two-parameter stability map was then obtained from the Cw, α, VT,max map. The results showed that this theoretical map can qualitatively prove the experimentally obtained results—the existence of different dispersed states of particles in suspension system and the variation tendency of suspension stability with changing each parame- ter—were reasonable.

关键词： 3.-TZP, suspension, stability, DLVO theory, interparticle potential energy.

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Role of gallium wetting layer in high-quality ZnO growth on sapphire (0001) substrates

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Science china(physics,Mechanics & Astronomy) 2004年第5期47卷 612-620页

作者： ZENG Zhaoquan1,3. WANG Yong2. DU Xiaolong1, MEI Zengxia1, KONG Xianghe3. JIA Jinfeng1, XUE Qikun1 & ZHANG Ze2. 4 1. State Key Laboratory for Surface {2., institute of {2., {3. Academy of sciences, Beijing 100080, china 2. Laboratory of Electron Microscopy, institute of {2., {3. Academy of sciences, Beijing 100080, china 3. College of {2. and Engineering, Qufu Normal University, Qufu 2.3.65, china 4. Beijing University of Technology, Beijing 10002., china State Key Laboratory for Surface physics Institute of Physics Chinese Academy of Sciences Beijing China College of physics and Engineering Qufu Normal University Qufu China Laboratory of Electron Microscopy Institute of Physics Chinese Academy of Sciences Beijing China Beijing University of Technology Beijing China

A Ga wetting layer was used to modify the surface structure of sapphire (0001) substrate to prepare high-quality ZnO film by radio frequency plasma-assisted molecule beam epitaxy. We found that this Ga layer plays a crucial role in eliminating 3. rotation domains, controlling polarity and decreasing defect density in ZnO epilayers, as demonstrated by in situ reflection high energy electron diffraction, ex situ high resolution X-ray diffraction and high resolution cross-sectional transmission electron microscopy. Zn-polar film of ZnO was determined by convergent beam electron diffraction. A Ga bilayer model is proposed to understand the effects of the Ga wetting layer on high-quality ZnO growth.

关键词： Ga wetting layer, sapphire, polarity, defect density, zinc oxide, rf-MBE, RHEED, HRXRD, TEM, CBED.

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Multiple Effects of energy Issues and Countermeasures: A Case of Shandong Province

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chinese Journal of Population,Resources and Environment 2008年第4期6卷 88-94页

作者： Wang Chengxin1,2., Wang Gefang3., Yao Shimou4 1. College of Population, Resources and Environment, Shandong Normal University, Jinan Shandong 2.0014, china 2. Research Center of Cycle Economy of Shandong Province, Jinan Shandong 2.0001, china 3. Party School of the Shandong Committee of CPC, Jinan Shandong 2.002., china 4. Nanjing {3. of Geography and Limnology, chinese Academy of sciences, Nanjing Jiangsu 2.0008, china College of Population Resources and Environment Shandong Normal University Jinan Shandong 250014 China Research Center of Cycle Economy of Shandong Province Jinan Shandong 250001 China Party School of the Shandong Committee of CPC Jinan Shandong 250021 China Nanjing institute of Geography and Limnology Chinese Academy of Sciences Nanjing Jiangsu 210008 China

energy is now becoming the bottleneck of urban and regional development. The paper takes Shandong Province, one of the most energy consuming provinces in{2., as an example, uses quantity methods such as SPSS (Stastics Package for Social Science) cluster sampling and regression analysis, and applies quantity analysis, to analyze the situation of energy issues in Shandong province. The conflicts between the quick industrialization and increasing extensive economic development, between urban population and energy consuming per head, between the infinite energy consuming desire and the low level of managing system, and between the unstable world energy supply and the limited energy storage in{2. are the main factors of energy crisis in Shandong, which not only results in energy shortage, but also becomes the barrier of economic and social development. In order to ensure sufficient energy supply for Shandong province in the 2.st century, we should take countermeasures, such as changing the economic development model, pursuing a multiple and {12. quality energy strategy, improving the intensive urbanization strategy, and building a scientific energy system.

关键词： energy effects countermeasure Shandong Province

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β-delayed proton decays near the proton drip line

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Science china(physics,Mechanics & Astronomy) 2005年第3期48卷 257-267页

作者： XU Shuwei 1 , LI Zhankui 1 , XIE Yuanxiang 1 , HUANG Wenxue 1 , SHU Nengchuan 2., CHEN Yongshou 2., XU Furong 3., WANG Kun 4 , PAN Qiangyan 1 , WANG Xudong 1 , YU Yong 1 & XING Yebing 1 1. institute of Modern {2., chinese Academy of {3., Lanzhou 73.000, china 2. Nuclear {2. Department, china institute of Atomic energy, Beijing 102.13. china 3. Department of Technical {2., Peking University, Beijing 100871, china 4. Shanghai institute for Nuclear Research, Shanghai 2.1800, china institute of Modern physics Chinese Academy of Sciences Lanzhou China Nuclear physics Department China Institute of Atomic Energy Beijing China Department of Technical physics Peking University Beijing China Shanghai institute for Nuclear Research Shanghai China

We briefly reviewed the experimental study on a-delayed proton decays near the proton drip line published by our group during the period of 1996―2.04, namely the first observation of the a-delayed proton decays of 9 new nuclides in the rare-earth region and the new measurements of a-delayed proton decays of 5 nuclides in the mass ～90 region near the N = Z line with the aid of the “p-?” coincidence in combination with a He-jet tape transport system. In the meantime some important experimental technique details were supplemented. The experimental results, including the half -lives, spins, parities, deformations and production reaction cross sections for the 14 nuclei were summarized and compared with the current nuclear-model predictions, and then the following points were represented. (1) The experimental half-lives for 85Mo and 92.h as well as the predicted “waiting point” nuclei 89Ru and 93.d are 5―10 times longer than the theoretical predictions given by M?ller et al. using a macroscopic-microscopic model. It considerably influences the predictions of the abundances of the nuclides produced in the rp-process. (2. The current-model predictions are not consistent with the experimental assignments of the spins and parities for the proton drip-line nuclei 142.o and 12.Pm. However, the nuclear potential energy surface (PES) calculated by using a Woods- Saxon-Strutinsky method reproduced the experimental results. (3. The Alice code overestimated the production reaction cross sections of the studied 9 rare-earth nuclei by one order of magnitude or two, while HIVAP code overestimated them by one order of magnitude approximately.

关键词： proton drip line, β-delayed proton decay, He-jet tape transport system, "p-γ" coincidence, spin, parity, deformation, production reaction cross section.

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Ultrafast excitation relaxation in light-harvesting complex LH2.from *** 601

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Science china Chemistry 2004年第3期47卷 192-199页

作者： GUO Lijun1,2. LIU Yuan3. LIU Weimin2. GUO Junhua1, XU Chunhe3.& QIAN Shixiong2.1. School of {2. and Information Optoelectronics, Henan University, Kaifeng 475001, china 2. {2. Department, Fudan University, Shanghai 2.043., china 3. Shanghai institute of Plant Physiology, chinese Academy of sciences, Shanghai 2.003., china School of physics and Information Optoelectronics Henan University Kaifeng China physics Department Fudan University Shanghai China Shanghai institute of Plant Physiology Chinese Academy of Sciences Shanghai China

The energy relaxation and kinetic evolution of transient spectra of bacteriochloro- phylls (BChls) in light-harvesting complex LH2.from Rb. sphaeroides 601 were investigated using femtosecond pump-probe technique. Upon 783.nm excitation, the energy at B800 BChls ex-periences an intramolecular redistribution with 0.3. ps time constant before transferring to B850 BChls. With tuning the excitation wavelength, the dynamical evolution of excited BChls was clearly observed, which indicates an obvious competition between the ground state bleaching and excited state absorption (ESA) of BChls involved and an isosbestic point near 818 nm, and also demonstrates that from the lower electronic excited state of B800 BChls to the higher exci-tonic state of B850 BChls is an efficient routine for energy transfer. The excitation energy in higher excitonic states of B850 BChls relaxes rapidly to the next lowest excitonic state by inter-conversion, delocalization to adjacent molecular, populating the lowest excitonic state and the change of molecular conformation.

关键词： photosynthesis, light-harvesting complex, energy transfer, ultrafast, dynamics, purple bacteria.

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xF(or y)-Dependence of Nuclecir Absorption and energy Loss Effects on J/ψ Production

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Communications in Theoretical physics 2009年第12期52卷 1049-1054页

作者： WANG Hong-Min,~(1,*) SUN Xian-Jing,~2.and ZHANG Ben-Ai~3. physics Department, Academy of Armored Forces Engineering of PLA, Beijing 100072.{2.2.3}, {3. Academy of sciences, Beijing 100049,{2.3.institute of Applied physics and Computation Mathematics, Beijing 100088,{2.

By means of the nuclear parton distribution studied only with lepton deep-inelastic scattering experimentaldata,the J/φ"normal nuclear absorption"and energy loss effects are studied in a Glauber formalism at HERA andRHIC *** that the absorption cross section σincreases with the charmonium-nucleon center of massenergy,the results reveal a significant dependence of the σon rapidity y at RHIC *** initial-state energyloss effect,which is found important only at HERA energies,is also considered,and its influence should be eliminatedwhen we studied the absorption effect at low collision ***,we also present the theoretical prediction forLHC.

关键词： x_F (or y)-dependence of absorption effect Glauber formalism energy loss

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Electronic structure and magnetism of R(Fe,Si)_(12. (R=Y,Nd)

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Science china(physics,Mechanics & Astronomy) 2006年第2期49卷 195-202页

作者： SUN Guang’ai1, CHEN Bo1, DU Honglin2. FAN Zhijian1, GAO Tao3.& QI Xinhua3.. {2. of Nuclear physics and Chemistry, china Academy of Engineering physics, Mianyang 62.900, china 2. Department of physics, Peking university, Beijing 100871, china 3. {2. of Atomic and Molecular physics, Sichuan University, Chengdu 610065, china institute of Nuclear physics and Chemistry China Academy of Engineering Physics Mianyang China Department of physics Peking university Beijing China institute of Atomic and Molecular physics Sichuan University Chengdu China

The newly developed full-potential linearized augmented plane wave (LAPW) and local orbitals (lo) based on standard APW methods are briefly introduced, and the structure and magnetic properties of R(Fe, Si)12.compounds (R = Y, Nd) are calculated using the method. The distribution of Si at different sites is analyzed based on total energy of one crystal unit with structure having been optimized. The characters of magnetic mo-ments, total density of states (TDOS) and partial density of states (PDOS) for different crystal sites Si occupies are obtained and analyzed. The results show that the total mag-netic moments of RFe10Si2.(R = Y, Nd) are larger than those of RFe10M2.(M = Ti, V, Cr, Mn, Mo and W) and the hybridization mechanism is seen as follows. Si(8j) reduce the mag-netic moments of Fe at three sites, however, Si(8f) mainly reduce the magnetic moments of Fe(8i) and Fe(8j) atoms. The Curie temperature is markedly enhanced by the introduc-tion of Si atoms according to spin fluctuation of DOS at Fermi level.

关键词： (L)APW+lo method R(Fe Si)12.R = Y Nd) magnetic moment Density of state.

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Fuzzy cluster analysis of the provenance of ancient Yaozhou porcelain body

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chinese Science Bulletin 2003年第1期48卷 27-30页

作者： LI Guoxia1, ZHAO Weijuan1, LI Rongwu2. XIE Jianzhong1, FENG Songlin3. FAN Dongyu3. ZHANG Ying3. CAI Zhifang3. ZHUO Zhenxi4 & GAO Zhengyao1 1. Faculty of Physical Engineering, Zhengzhou University, Zhengzhou 450052. china 2. Department of physics, Beijing Normal University, Beijing 100875, china 3. {3. energy physics institute, the {12. Academy of sciences, Beijing 100080, china 4. Archaeology Research institute, Shanxi Province, Xian 710000, china Correspondence should be addressed to Li Guoxia (e-mail: Liguoxia@ ***) Faculty of Physical Engineering Zhengzhou University Zhengzhou 450052 China Department of physics Beijing Normal University Beijing 100875 China high energy {2. {3. Chinese Academy of Sciences Beijing 100080 China Archaeology Research institute Shanxi Province Xi'an 710000 China

The technique of neutron activation analysis (NAA) has been employed to measure the content of 2. kinds of elements in the sample of Yaozhou porcelain bodies. Then a fuzzy cluster analysis has been conducted to the NAA data and a diagram of the dynamic fuzzy cluster analysis has been achieved. The results indicate that the batch of ancient Yaozhou porcelain bodies, which were of different overglaze color and were produced by different kilns during a period of over 800 years from the Tang Dynasty (618—907 A.D.) to the Yuan Dynasty (12.1—13.8 A.D.), has shared a stable and concentrated raw material source. Provenances of por-celain bodies from different times, though having their spe-cific independence, enjoy a close relationship and are not far from one another. Provenances of porcelain bodies made during the Tang Dynasty and the Five Dynasties (907—960 A.D.) are found to be closer to one another, while those of the Song (960—12.9 A.D.) and the Jin Dynasty (1115—12.0} A.D.) are comparatively concentrated in certain areas and are dif-ferent from those of the Tang Dynasty. Both the tri-colored glazed pottery made in Yaozhou kilns during the Tang Dy-nasty and the Yaozhou porcelain bodies of the Tang period are from the same provenance.

关键词：古代姚州瓷胎窑址粘土矿原料来源中子活化分布模糊聚类分析

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Narrow line Seyfert 1 galaxies: where are the broad line regions?

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Science china(physics,Mechanics & Astronomy) 2010年第12期53卷 2307-2311,2339-2343页

作者： MAO WeiMing 1,2.3., HU Chen 2., WANG JianMin 2., BIAN WeiHao 4 , ZHANG Shu 2.& ZHAO Gang 1 1 Key Laboratory of Optical Astronomy, National Astronomical Observatories, chinese Academy of {2., Beijing 100012. china 2.Key Laboratory for Particle Astro{3., institute of high energy physics, chinese Academy of {2., Beijing 100049, china 3.Graduate University of the chinese Academy of {2., Beijing 100049, china 4 Department of {3., Nanjing Normal University, Nanjing 2.0011, china Key Laboratory of Optical Astronomy National Astronomical Observatories Chinese Academy of Sciences Beijing China Key Laboratory for Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing China Graduate University of the chinese Academy of sciences Beijing China Department of physics Nanjing Normal University Nanjing China

A sample consisting of 2.1 narrow line Seyfert 1 galaxies (NLS1s) with high quality spectra from the Sloan Digital Sky Survey (SDSS) is selected to explore where broad line regions are in these objects. We find that the Hβ profile can be fitted well by three (narrow, intermediate and broad) Gaussian components, and the FWHM ratios of the broad to the intermediate components hold a constant of 3.0 roughly for the entire sample. If the broad components originate from the region scaled by the well-determined Hβ reverberation mapping relation, we find that the intermediate components originate from the inner edge of the torus, which is scaled by dust K-band reverberation. We find that the IC and the BC are strongly linked dynamically, but the relation of their covering factors is much more relaxed, implying that both regions are clumpy.

关键词： black hole physics galaxies: evolution quasars: general

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