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Rat CC chemokine receptor 4 is the functional homologue of human CC chemokine receptor 4 and can interact with human CCL17 and CCL22

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Chinese science Bulletin 2010年第14期55卷 1388-1395页

作者： TIAN LinJie1,2, QI Hui1,2, XIE Yuan1,2, ZHANG YingMei1,2, ZHANG WenJuan1,2, SUN XiangYu1,2, WANG Ying1,2 & MA DaLong1,2 1 Laboratory of Medical Immunology, School of Basic Medical science, peking university {3. science center, beijing 100191, china 2 Human Disease Genomics center, peking university, beijing 100191, china Laboratory of Medical Immunology School of Basic Medical Science Peking University Health Science Center Beijing 100191 China Human Disease Genomics center Peking University Beijing 100191 China

Human CCL17/TARC (hCCL17) and CCL22/MDC (hCCL22) interact with their receptor CCR4, playing pivotal roles in various Th2 cell-dominant diseases. Rat Ccl17, Ccl22 and Ccr4 share 63.4%, 65.2% and 87.5% homology at the amino acid level, respectively, compared with their human homologues. Rat Ccl22 has been demonstrated to interact with rat Ccr4. However, it is not known whether rat Ccl17 is a functional ligand for rat Ccr4 and whether rat Ccr4 can interact with hCCL17 and hCCL22. In this study, we cloned rat Ccl17 and Ccr4 cDNA from rat thymus and expressed the recombinant rat Ccl17. The binding assay indicated rat Ccl17 could saturably bind rat Ccr4, and this binding could be competitively inhibited by unlabeled rat Cc17, hCCL17 or hCCL22. Both rat and human CCL17 or CCL22 could induce the chemotactic migration and calcium mobilization in rat Ccr4-transfected cells. They could also desensitize each other’s effect on chemotaxis and calcium mobilization. Furthermore, both rat Ccl17 and hCCL17 could induce significant receptor internalization in rat Ccr4-EGFP transfected cells. These results demonstrated the conserved CCL17/CCR4 interaction in rats and the further cross-species interaction of rat Ccr4 with hCCL17 and hCCL22. Likewise, they provided the molecular basis for the investigation of physiological and pharmacological roles of CCR4, its ligands, and their antagonists in rat disease models.

关键词：趋化因子受体同源性大鼠人类基因转染细胞绿色荧光蛋白相互作用疾病模型

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Propagation of interplanetary shock excited ultra low frequency (ULF) waves in magnetosphere-ionosphere-atmosphere——Multi-spacecraft “Cluster” and ground-based magnetometer observations

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science china(Technological sciences) 2010年第9期53卷 2528-2534页

作者： WANG ChengRui1,2,ZONG QiuGang1,3.& WANG YongFu1 1 Institute of Space Physics and Applied Technology,peking university,beijing 100871,{3. 2 Sate Key Laboratory of Space Weather,Chinese Academy of sciences,beijing 100190,{3. 3.center for Atmospheric Research,600 Suffolk Street,university of Massachusetts Lowell,MA 01854,USA 1. Institute of Space Physics and Applied Technology Peking University Beijing 100871 China2. Sate Key Laboratory of Space Weather Chinese Academy of Sciences Beijing 100190 China3. Center for Atmospheric Research University of Massachusetts 600 Suffolk Street Lowell MA 01854 USA

The ultra low frequency (ULF) wave in magnetosphere can act as an important means for solar wind energy inward *** paper quantitatively analyzes the propagation process of the ULF wave triggered by the interplanetary shock propagating from inner magnetosphere equatorial plane along magnetic field lines to the top of the ionosphere and below ionosphere propagating process and establishes a relatively complete magnetosphere-ionosphere-atmosphere propagation model which can be used to study the relationship between the amplitude of the ULF waves triggered by the interplanetary shock wave in magnetospheric space and the magnetic effect caused by the ULF *** a comparison with recent observations,we found that: in the event during November 7,2004 that an interplanetary shock wave interacted with the magnetosphere,Cluster satellites observed that electric field fluctuations and the band-pass filtered result of ground stations meridional component had similar *** with the geomagnetic measurement near the footprints,we found that the electric field disturbance in the magnetosphere spread along the ground magnetic field lines in the form of the ULF waves and changed into geomagnetic *** result reveals that the ULF wave is in contact with the ground geomagnetic *** ULF waves couple with ionized components in ionosphere and spread to the ground in the form of electromagnetic *** this research,we believe that the magnetosphere,ionosphere and ground magnetic effects caused by interplanetary shock wave are the same physical phenomena responding in different *** on the overall consideration of entire electromagnetic response to the interplanetary shock wave,we found that the correlation between CLUSTER multi-satellite observation and geomagnetic station observation is due to the ULF wave propagated in magnetosphere-ionosphere-atmosphere system,and we quantitatively interpreted this response process

关键词： ultra low frequency (ULF) wave magnetic storm ionosphere IRI model interplanetary shock

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Yeast two-hybrid screening for proteins that interact with α1-adrenergic receptors

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Acta Pharmacologica Sinica 2004年第11期 87-94页

作者： Tan ZHANG, Qi XU, Feng-rong CHEN, Qi-de HAN, You-yi ZHANGInstitute of Vascular Medicine, peking university Third Hospital and Key Laboratory of Molecular cardiovascular {3.,Ministry of Education, beijing 100083, chinaNow in Department of Cardiology, peking university Third Hospital, beijing, china.

AIM: To find novel proteins that may bind to α1A-adrenergic receptor (α1A-AR) and investigate their interactions with the other two α1-AR subtypes (α1B-AR and α1D-AR) with an expectation to provide new leads for the function study of the receptors. METHODS: Yeast two-hybrid assay was performed to screen a human brain cDNA library using the C terminus of α1A-AR (α1A-AR-CT) as bait. X-Gal assay and o-nitrophenyl-beta-D-galactopyranoside (ONPG) assay were subsequently conducted to further qualitatively or quantitatively confirm the interactions between receptors and the three identified proteins. RESULTS: (1) Selection medium screening identified segments of bone morphogenetic protein-1 (BMP-1), active Bcr-related protein (Abr), and filamin-C as binding partners of α1A-AR-CT in yeast cells respectively. Besides, protein segments of BMP-1 and Abr could only specifically interact with α1A-AR-CT while filamin-C segment interacted with all three α1-AR subtypes. (2) In X-Gal assay, the co-transformants

关键词： active Bcr-related protein α1-adrenergic receptors bone morphogenetic protein-1 filamin-C yeasts two-hybrid system techniques beta-galactosidase

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The PKG mechanism of nitric oxide-induced vasodilatation

The PKG mechanism of nitric oxide-induced vasodilatation

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11th International Vascular Neuroeffector Mechanisms and cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006

作者： Yuansheng Gao peking university health {3. center, beijing, china

Cyclic GMP-dependent protein kinase (PKG) plays a critical role in vasodilation induced by cGMP-elevating agents such as nitric oxide, nitrovasodilators, and natriuretic peptides. In mammalian cells, PKG is present in two forms, PKG I and PKG Ⅱ. The type Ⅰ isoform is the primary enzyme involved in cGMP-dependent regulation of cardiovascular function. PKG may cause vasodilation through the reduction of cytosolic Ca2+ level by stimulating calcium-activated potassium channels, by interfering with the generation of inositol

关键词： PKG The PKG mechanism of nitric oxide-induced vasodilatation

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Correlative ionospheric disturbances in the sunlit hemisphere during the flare on July 14,2000

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Chinese science Bulletin 2002年第12期47卷 975-977页

作者： ZHANG Donghe & XIAO ZuoDepartment of Geophysics, peking university, beijing 100871, {3. Qingdao Research center, Chinese Research Institute of Radio-wave Propagation, Qingdao 266071, {3. center for Space science and Applied Research, Chinese Academy o Department of Geophysics Peking University Beijing China Qingdao Research center Chinese Research Institute of Radio-wave Propagation Qingdao China center for Space science and Applied Research Chinese Academy of Sciences Beijing China

Using GPS data obtained from GPS sites located on the sunlit hemisphere during the flare on July 14, 2000, the ionospheric response to the flare was analyzed. A kind of tiny correlative ionospheric fluctuations were revealed from the temporal total electron content (TEC) curves derived from GPS measurements, and the values of this kind of disturbances are about 1015 m-2. A comparative analysis of these disturbances and soft X-ray flux detected at GOES satellite during the flare is done. It is found that this kind of disturbances revealed in the temporal TEC curves did occur in a very large area of sunlit ionosphere and its behavior is mainly controlled by the characteristics of the flare’s extra radiation. Because the similar fluctuations are not found in the curve of soft X-ray flux, it is concluded that the flare extra radiation in EUV band should have corresponding fluctuations and it is the fluctuations that cause the TEC disturbances. The height, where the disturbances occur, should be in the F

关键词： GPS TEC flare correlative disturbance.

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Hormones and cancer

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Cell Research 2007年第4期17卷 277-279页

作者： Yongfeng Shang~1 ~1Department of Biochemistry and Molecular Biology,peking university health {3. center,3. Xueyuan Rd,beijing 100083.china DepartmentofBiochemistryandMolecularBiology PekingUniversityHealthScienceCenter38XueyuanRdBeijing100083China

Estrogen exhibits a broad spectrum of physiologicalfunctions ranging from regulation of the menstrual cycleand reproduction to the modulation of bone density,brainfunction,and cholesterol ***,estrogenis also associated with pathological complications par-ticularly with the onset of gynaecological malignanciesincluding breast cancer and endometrial cancer;estrogenis now considered to be a classical etiological factor forbreast cancer and endometrial *** the early 1970s,it was reported that there was a 20-3.% increase in incidence of endometrial cancer in WesternCaucasian women who had undergone estrogen therapy[1].Subsequently,a variety of clinical and epidemiologi-cal investigations,with support from studies in cell cultureand animal models,have demonstrated the association ofestrogen with the development and/or progression of *** 2002,the US National Toxicology Programlisted steroidal estrogens as carcinogens for the first *** report cites data from human epidemiologic studies thatshow an association between estrogen replacement therapyand an increase in the risk of endometrial cancer,as well asa less consistent increase in the risk of breast cancer.

关键词： gene NSABP Hormones and cancer ERE SERMs

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Inhibition of angiotensin Ⅱ and blockade of endothelin receptors reduce arterial calcification in rats

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Journal of Geriatric Cardiology 2004年第2期1卷 108-113页

作者： Juxiang LI~1 Shengying WU~1 Chunshui PAN~2 Yongfen QI~2 Bin GENG~1 Xiuhna LIU~~(1,2) Chaoshu TANG~1 1 Department of Physiology and Pathophysiology,peking university health {3. center,beijing 100083.china 2 Insitiute of cardiovascular Research,peking university First Hospital,beijing 10003.,china Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China Insitiute of cardiovascular Research Peking University First Hospital Beijing 100034 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China Insitiute of cardiovascular Research Peking University First Hospital Beijing 100034 China

Objective To examine whether the two vascular paracrine/autocrine factors,angiotensin Ⅱ(Ang Ⅱ)and endothelin,participate in the pathogenesis of arterial *** Nicotine and vitamin D_3.treated rats were *** calcification was confirmed by using Von Kossa staining,measurement of calcium content, ^(45)Ca^(2+)uptake assay and alkaline phosphatase(ALP)*** plasma and vascular Ang Ⅱ and endothelin levels were measured by using *** and endothehn mRNA levels were determined by RT- *** The arterial calcium content,^(45)Ca^(2+)uptake and ALP activity were increased in calcification groups compared with control(P3.)

关键词： aorta angiotensin Ⅱ endothelin-1 calcification

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Expression,purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells

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World Journal of Gastroenterology 2003年第4期9卷 678-682页

作者： Bing Li Hong-Yan Wu Xiao-Ping Qian Yan Li Wei-Feng Chen Immunology Department of peking university health {3. center,beijing,100083.china Immunology Department of peking university health {3. center 38 Xueyuan Road Beijing 100083 China. wfchen@***

AIM: In order to assess hepatocellular carcinoma associated antigen HCA587 as a potential target for immunotherapy,the Bac-to-Bac expression system was used to express recombinant protein HCA587 in insect ***: The cDNA encoding HCA587 gene was cloned into donor vector pFasBacHtb and recombinant pFasBac Htb587 was transformed into competent cells *** Bacmid-587 was transfected into Sf9 insect cells using CELLFECTIN, Recombinant HCA587 protein was produced in Sf9 insect cells after infection with recombinant baculovirus, and was purified using Ni-NTA resin. Sera from HCC patients were also screened using recombinant protein ***: The molecular weight of the recombinant protein HCA587 expressed in insect cells was approximately *** blot results proved the recombinant protein HCA587had the similar antigenicity with its native *** analysis told that the rate of seroreactivity to HCA587 was not high in HCC ***: The recombinant protein HCA587 was successfully expressed and purified using Bac-to-Bac expression system. It paved the way for generation of specific antibody and investigation of immunohistochemical analysis and immune responses of HCC in the future.

关键词： HCA587抗原肝细胞癌血清学检查免疫疗法免疫组织化学

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Explanation of the mechanism of carcinogenesis and syntheses of anticancer agents with high selectivity

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science china Chemistry 2006年第1期49卷 44-62页

作者： DAI Qianhuan1,2 1. center of Chemistry and Bioengineering of Cancer Research, beijing Polytechnic university (Now known as beijing Univer-sity of Technology), beijing 100022, china 2. center of Environmental sciences, peking university, beijing 100871, china 1. center of Chemistry and Bioengineering of Cancer Research Beijing Polytechnic University Beijing 100022 China 2. center of Environmental sciences Peking University Beijing 100871 China

In 1979, the mechanism of chemical carcinogenesis, a challenging anddifficult scientific problem pending for a number of years, was explained by Dai Qianhuan. Themechanism named di-region theory predicted that a carcinogen always metabolizes to form a specialbi-functional alky-lating agent. This agent induces cross-linkages between the complementary basepairs in DMA and switches on initial mutageneses in genomes including point and frameshiftmutations. This, in turn, induces further deep mutageneses including the production of variouschimeric chromosomes, deletions and other aberrations found in genomes. In the end this initiatescarcinogenesis of the whole cell through the reverse transcription mechanism after a lengthyincubation period. Recently, this laboratory has verified that physical carcinogenesis, includingthe oncogenesis induced by radiation and asbestos as well as the carcinogenesis induced byendogenous factors such as estrogen or diethyl-stilbestrol switch on carcinogenesis by inducing theformation of cross-linkages between the complementary base pairs in DNA. Di-region theory has nowbeen supported by many experimental observations such as mutational spectra of various *** potential for carcinogenesis, teratogenesis, sterility and mutagenesis lumped together asgenetic toxicity appears to originate almost uniformly from the cross-linking between complementarybases, i.e. malignant cross-linking, which is in accordance with di-region theory. Other forms ofcross-linking between non-complementary bases, benign cross-linkings, show bi-functional alkylationanticancer activity but lack genetic toxicity. The predictable design and synthesis of a highselectivity anticancer agent with high efficacy and low genetic toxicity, a goal long pursued incancer chemotherapy, have been realized for the first time in this laboratory by inhibitingmalignant and heightening benign cross-linking using the principles of di-region theory. A series ofpatented new anticanc

关键词： mechanism of carcinogenesis, di-region theory, minoplatin, selectivity of anticancer agent, cross-linking between DNA bases, anticancer platinum complexes, predictive design of anticancer agent, second generation anticancer agent.

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Enhancement of cardiomyocyte differentiation from human embryonic stem cells

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science china(Life sciences) 2010年第5期53卷 581-589页

作者： WANG YanXia1,2,CHEN GuiAn1,2,SONG TianRan1,2,MAO GenHong1,2 & BAI HaiYan1,2 1Reproductive Medicine center,peking university Third Hospital,{3. 100083.china 2Stem Cell Research center,peking university Third Hospital,{3. 100083.china 1. Reproductive Medicine center Peking University Third Hospital Beijing 100083 China2. Stem Cell Research Center Peking University Third Hospital Beijing 100083 China

Several approaches have been used to encourage the differentiation of cardiomyocytes from human embryonic stem ***,the differentiation efficiency is low,and appropriate culture protocols are needed to produce adequate numbers of cardiomyocytes for therapeutic cell *** study investigated the effects of serum on cardiomyocyte differentiation in suspension culture medium during embryoid body(EB) formation by human embryonic stem *** addition of ascorbic acid,dimethylsulfoxide and 5-aza-2'-deoxycytidine during days 5-7 at the EB-forming stage resulted in an increase in the numbers of rhythmically contracting clusters of derived *** with 0.1 mmol L-1 ascorbic acid alone,or more notably in combination with 10 μmol L-1 5-aza-2'-deoxycytidine,induced the formation of beating cells within *** of the beating clusters had spontaneous contraction rates similar to those found in human adults,and their contractile ac-tivity lasted for up to 194 days.

关键词： human embryonic stem cells cardiomyocyte differentiation serum ascorbic acid 5-aza-dC

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