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Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice

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science China(Life sciences) 2008年第11期51卷 973-980页

作者： LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 Xiangya School of Medicine, Central South university, Changsha 410078, China 2 Department of Microbiology and Immunology, university of South China, Hengyang 421001, China 3 university of texas health science center at san antonio, tx 78229, usa Xiangya School of Medicine Central South University Changsha China Department of Microbiology and Immunology University of South China Hengyang China university of texas health science center at san antonio San Antonio USA

To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5 DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5 antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5 vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5 induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5 protein or the inactive elementary body in vitro. pcDNA3.1-pORF5-vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5 vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5-immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P<0.05). Together, these results demonstrated that immu- nization with pORF5 DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge.

关键词： Chlamydia trachomatis pORF5 DNA vaccine immune protection Th1 immune response

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Immunization with Chlamydial Plasmid Protein pORF5 DNA Vaccine Induces Protective Immunity against Genital Chlamydial Infection in Mice

Immunization with Chlamydial Plasmid Protein pORF5 DNA Vacci...

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2008年中国微生物学会学术年会

作者： Li Zhong-Yu~(1)),Wu Yi-Mou~(1)),WANG Shi-Ping~(2)),ZHONG Guang-Ming~(3)),CHEN Ding~(3)) 1) Department of Microbiology and Immunology,university of South China,Hengyang 421001,China 2) Xiangya School of Medicine,Central South university,Changsha 410078,China 3) university of texas health science center at san antonio,tx 78229,usa

To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this *** this study,pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial *** of BALB/c mice were immunized intranasally with pORF5 DNA *** and cell mediated immune responses were evaluated.

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A PROBABILISTIC CHARACTERIZATION OF A FAULT-TOLERANT GOSSIPING ALGORITHM

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Journal of Systems science & Complexity 2009年第1期22卷 88-108页

作者： Paul PARKER Department of Computer science University of Texas at San Antonio One UTSA Circle

Gossiping is a popular technique for probabilistic reliable multicast （or broadcast）. However, it is often difficult to understand the behavior of gossiping algorithms in an analytic fashion. Indeed, existing analyses of gossip algorithms are either based on simulation or based on ideas borrowed from epidemic models while inheriting some features that do not seem to be appropriate for the setting of gossiping. On one hand, in epidemic spreading, an infected node typically intends to spread the infection an unbounded number of times （or rounds）; whereas in gossiping, an infected node （i.e., a node having received the message in question） may prefer to gossip the message a bounded number of times. On the other hand, the often assumed homogeneity in epidemic spreading models （especially that every node has equal contact to everyone else in the population） has been silently inherited in the gossiping literature, meaning that an expensive mcnlbership protocol is often needed for maintaining nodes＇ views. Motivated by these observations, the authors present a characterization of a popular class of fault-tolerant gossip schemes （known as ＂push-based gossiping＂） based on a novel probabilistic model, while taking the afore-mentioned factors into consideration.

关键词： Fault-tolerance gossip probabilistic broadcast reliable multicast.

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Molecular signal transduction in vascular cell apoptosis

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Cell Research 2001年第4期11卷 253-264页

作者： GENG YONG JIAN center for Cardiovascular Biology and Atherosclerosis Research, Division of Cardiology, Department of Internal Medicine, university of texas Houston health science center Medical School, 6431 Fannin Street, MSB 6.045, Houston, tx 77030, usa Division of Cardiology Department of Internal Medicine Center for Cardiovascular Biology and Atherosclerosis Research University of Texas Houston Health Science Center Medical School Houston USA

Apoptosis is a form of genetically programmed cell death, which plays a key role in regulation of cellularity in a variety of tissue and cell types including the cardiovascular tissues. Under both physiological and pathophysiological conditions, various biophysiological and biochemical factors, including mechanical forces, reactive oxygen and nitrogen species, cytokines, growth factors, oxidized lipoproteins, etc., ma influence apoptosis of vascular cells. The Fas/Fas ligand/caspase death-signaling pathway, Bcl-2 protein family/mitochondria, the tumor suppressive gene p53, and the proto-oncogene c-myc may be activated in atherosclerotic lesions, and mediates vascular apoptosis during the development of atherosclerosis. Abnormal expression and dysfunction of these apoptosis-regulating genes may attenuate or accelerate vascular cell apoptosis and affect the integrity and stability of atherosclerotic plaques. Clarification of the molecular mechanism that regulates apoptosis may help design a new strategy for treatment of atherosclerosis and ii major complication, the acute vascular syndromes.

关键词： Apoptosis vascular cells caspases atherosclerosis cytokines.

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Oncogenesis of RON receptor tyrosine kinase:a molecular target for malignant epithelial cancers

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Acta Pharmacologica Sinica 2006年第6期27卷 641-650页

作者： Ming-hai WANG~(2,3,4)Hang-ping YAO~2 Yong-qing ZHOU~2 2 Laboratory of Chang-Kung Scholars Program for Tumor Biology,First Affiliated Hospital,Zhejiang university School of Medicine,Hangzhou 310003.China 3 Cancer Biology center and Department of Pharmaceutical sciences,texas Tech university health sciences center School of Pharmacy,Amarillo,tx 79106,usa Laboratory of Chang-Kung Scholars Program for Tumor Biology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou China Cancer Biology center and Department of Pharmaceutical sciences Texas Tech University Health Sciences Center School of Pharmacy Amarillo USA

Recepteur d’origine nantais(RON)belongs to a subfamily of receptor tyrosinekinases(RTK)with unique expression patterns and biological *** isactivated by a serum-derived growth factor macrophage stimulating protein(MSP).The RON gene transcription is essential for embryonic development and critical inregulating certain physiological *** studies have indicated thataltered RON expression contributes significantly to cancer progression *** primary tumors,such as colon and breast cancers,overexpressionof RON exists in large numbers and is often accompanied by the generation ofdifferent splicing *** RON variants direct a unique program thatcontrols cell transformation,growth,migration,and invasion,indicating that al-tered RON expression has the ability to regulate motile/invasive *** activities were also seen in transgenic mice,in which targeted expression ofRON in lung epithelial cells resulted in numerous tumors with pathological fea-tures of human bronchioloalveolar ***,abnormal RON activation isa pathogenic factor that transduces oncogenic signals leading to uncontrolledcell growth and subsequent malignant *** these facts,RON and its variants can be considered as potential targets for *** using small interfering RNA and neutralizing mono-clonal antibodies demonstrated that suppressing RON expression and activationdecreases cancer cell proliferation,increases apoptotic death,prevents tumorformation in nude mice,and reduces malignant ***,blocking RONexpression and activation has clinical significance in reversing malignant pheno-types and controlling tumor growth.

关键词： growth factor receptor signal transduction tumorigenesis monoclonal antibody small molecule inhibitor therapeutics

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AN INTERIOR TRUST REGION ALGORITHM FOR NONLINEAR MINIMIZATION WITH LINEAR CONSTRAINTS

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Journal of Computational Mathematics 2002年第3期20卷 225-244页

作者： Jian-guo Liu (Department of Mathematics, university of North texas, Denton, tx 76203, usa) Department of Mathematics University of North Texas 美国 Denton

Presents information on a study which analyzed an interior trust-region-based algorithm for linearly constrained minimization problems. Optimality conditions for the linearly constrained minimization problem presented; Vectors for each updating step in the algorithm proposed; Establishment of the convergence properties of the proposed algorithm.

关键词： Nonlinear programming Linear constraints Trust region algorithms Newton methods Interior algorithms Quadratic convergence

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fMRI time series analysis based on stationary wavelet and spectrum analysis

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Progress in Natural science:Materials International 2006年第11期16卷 1171-1176页

作者： ZHI Lianhe~ 1,2,3 , ZHAO Xia~4, SHAN Baoci~1**, PENG Silong~5, YAN Qiang~1, YUAN Xiuli~1 and TANG Xiaowei~ 1,6 (1. Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of sciences, Beijing 100049, China 2. Graduate School of Chinese Academy of sciences, Beijing 100049, China 3. Department of Physics, Zhoukou Normal university, Zhoukou 466100, China 4. Research Imaging center, university of texas health science center, san antonio, texas 78229, usa 5. National ASIC Design and Engineering center, Institute of Automation, Chinese Academy of sciences, Beijing 100049, China 6. Department of Physics, Zhejiang university, Hangzhou 310027, China) Key Laboratory of Nuclear Analysis Techniques Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Graduate School of Chinese Academy of sciences Beijing 100049 China Department of Physics Zhoukou Normal University Zhoukou 466100 China Research Imaging center University of Texas Health Science Center San Antonio TX 78229 United States National ASIC Design and Engineering center Institute of Automation Chinese Academy of Sciences Beijing 100049 China Department of Physics Zhejiang University Hangzhou 310027 China

The low signal to noise ratio (SNR) of functional MRI (fMRI) prefers more sensitive data analysis methods. Based on stationary wavelet transform and spectrum analysis, a new method with high detective sensitivity was developed for analyzing fMRI time series, which does not require any prior assumption of the characteristics of noises. In the proposed method, every component of fMRI time series in the different time-frequency scales of stationary wavelet transform was discerned by the spectrum analysis, then the components from noises were removed using the stationary wavelet transform, finally the components of real brain activation were detected by cross-correlation analysis. The results obtained from both simulated and in vivo visual experiments illustrated that the proposed method has much higher sensitivity than the traditional cross-correlation method.

关键词： fMRI stationary wavelet transform spectrum analysis data analysis.

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Limbic leptin signaling and depression

Limbic leptin signaling and depression

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xin-Yun LU Departments of Pharmacology and Psychiatry,university of texas health science center at san antonio,7703 Floya Curl Drive,san antonio,tx 78229,usa

＜正＞Major depressive disorder is the most common mental illness,affecting roughly 20%of the general population at some point during their *** approximately half of patients respond to existing antidepressant ***,the mechanisms underlying the pathogenesis of depression remain poorly *** is often comorbid with medical conditions such as ***,an adipocyte-derived hormone,is well known for its role in the control of energy homeostasis and body *** research has provided evidence for a novel action of leptin in the regulation of mood and *** leptin levels are reduced in chronic stress models of *** treatment reverses chronic stress-induced depression-like behavioral deficits and impairment of hippocampal *** addition,we have explored limbic leptin signaling in mood-related *** implications of leptin and leptin receptor signaling in human depression will be discussed.

关键词： depression leptin stress hippocampus

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A Spatially Weighted Degree Model for Network Vulnerability Analysis

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Geo-Spatial Information science 2011年第4期14卷 274-281页

作者： WAN Neng1,ZHAN F.Benjamin1,2,CAI Zhongliang2 1.School texas center for Geographic Information science,Department of Geography,texas State university,san Marcos,tx 78666,usa 2.School of Resource and Environmental science,Wuhan university,129 Luoyu Road,Wuhan 430079,China School texas center for Geographic Information science Department of Geography Texas State University San Marcos USA School of Resource and Environmental science Wuhan University Wuhan China

Using degree distribution to assess network vulnerability represents a promising direction of network ***,the traditional degree distribution model is inadequate for analyzing the vulnerability of spatial networks because it does not take into consideration the geographical aspects of spatial *** paper proposes a spatially weighted degree model in which both the functional class and the length of network links are considered to be important factors for determining the node degrees of spatial networks.A weight coefficient is used in this new model to account for the contribution of each factor to the node *** proposed model is compared with the traditional degree model and an accessibility-based vulnerability model in the vulnerabil-ity analysis of a highway *** results indicate that,although node degrees of spatial networks derived from the tra-ditional degree model follow a random distribution,node degrees determined by the spatially weighted model exhibit a scale-free distribution,which is a common characteristic of robust *** to the accessibility-based model,the proposed model has similar performance in identifying critical nodes but with higher computational efficiency and better ability to reveal the overall vulnerability of a spatial network.

关键词： GIS network analysis spatial analysis vulnerability analysis

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Climate change tendency and grassland vegetation response during the growth season in Three-River Source Region

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science China Earth sciences 2010年第10期53卷 1506-1512页

作者： QIAN Shuan1,FU Yang2 & PAN FeiFei3 1 National Meteorological center,Beijing 100081,China 2 Qinghai Meteorological Research Institute,Xining 810001,China 3 Department of Geography,university of North texas,Denton,tx 76203,usa National Meteorological center Beijing China Qinghai Meteorological Research Institute Xining China Department of Geography University of North Texas Denton USA

Climate change,climate suitablity change for grassland vegetation,and grassland response to climate change during the growth season are studied systematically by using meteorological data of temperature,precipitation,and length of sunlight from 1961 to 2007,NOAA/AVHRR NDVI from 1982 to 2006,and observations of grass height,biomass,and coverage in enclosed grassland from 1994 to *** models are developed to evaluate climate change and the climatic suitability of grassland vegetation *** temperature,accumulative precipitation,and length of sunlight during the growth season increased from 1961 to 2007 at rates of 0.24°C/10 yr,2.32 mm/10 yr,and 2.81 h/10 yr,*** increase rates of ave-rage temperature between April and May,and between June and August are 0.17 and 0.30°C/10 yr;accumulative rainfall between April and May increased at a rate of 2.80 mm/10 yr,and accumulative rainfall between June and August decreased at a rate of 0.38 mm/10 yr;the increase rates of accumulative length of sunlight between April and May,and between June and August are 2.15 and 1.2 h/10 *** climatic suitability of grassland vegetation growth showed an increasing trend,and the increase rate between April and May is greater than that between June and *** NDVI observations from 1982 to 2006 and measurements of grass height,dry biomass,and coverage from 1994 to 2006 show that the climate change has resulted in an increase in plant productivity of the grassland in Three-River Source *** some protection measures are taken as soon as possible,the deteriorated grassland in Three-River Source Region can recover.

关键词： climate change climate suitability change grassland vegetation Three-River Source Region

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