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Chinese Science Bulletin 1999年第4期44卷 348-351页

作者： JIANG Shuye, CHEN Qifeng and FANG Xuanjun Biotechnology research Center, Chinese academy of agricultural Science, Beijing 100081, china institute of Genetics and Crop Breeding, Fujian agricultural University, Fuzhou 350002, china Corresponding author Biotechnology research Center Chinese Academy of Agricultural Science Beijing China institute of Genetics and Crop Breeding Fujian Agricultural University Fuzhou China

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility *** populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.

关键词： photo-period sensitive genie male sterile genes cDNA-RAPD differential display analysis.

Monitoring of rice crop using ENVISAT ASAR data

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Science china Earth sciences 2006年第7期49卷 755-763页

作者： DONG Yanfang1, 2, SUN Guoqing1,3 & PANG Yong4 1. State Key Laboratory of Remote Sensing Science, institute of Remote Sensing Applications, Chinese academy of sciences, Beijing 100101, china 2. Graduate University of Chinese academy of sciences, Beijing 100039, china 3. Department of Geography, University of Maryland, College Park, MD 20742, USA 4. Forest Remote Sensing Lab, research institute of Forest Resource and Information Technology, Chinese academy of For-estry, Beijing 100091, china 1. State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing 100101 China 2. Graduate University of Chinese academy of sciences Beijing 100039 China 3. Department of Geography University of Maryland College Park MD 20742 USA 4. Forest Remote Sensing Lab Research Institute of Forest Resource and Information Technology Chinese Academy of Forestry Beijing 100091 China

Because of the cloudy conditions during the rice growth period, rice is one of the agri-cultural crops most suited to monitoring with the SAR instruments. Backscatter response measured by SAR is correlated with rice conditions, including height, density, biomass and structure, which are variable at different growing stages. In this paper, multi-date ENVISAT ASAR Alternating Polarization Mode (APMode) imageries were acquired during the rice crop growing cycle. At the same time, the rice parameters were measured in field. A continuous canopy model was used to compute the back-scattering from rice fields during the growth cycle, and the relationship between rice parameters and radar backscattering coefficients from both ASAR and modeling was analyzed. The effects of polari-zation, incidence angle and polarization on radar backscattering coefficients were analyzed. It was found that simulated radar backscatter has similar trends as ASAR data. This will be meaningful for the further research of rice parameters estimation from ASAR data. Different features show signifi-cantly different signatures in ASAR images and they follow some certain laws, so rice area can be accurately mapped by using multi-temporal SAR images, then rice yield can be estimated.

关键词： rice, ASAR, radar backscattering, model.

Contributions of Different N Sources to Crop N Nutrition in a Chinese rice Field

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Pedosphere 2010年第2期20卷 198-208页

作者： CHEN Yi,TANG Xu,YANG Sheng-Mao,WU Chun-Yan and WANG Jia-Yu institute of Environmental Resources and Soil Fertilizer,Zhejiang academy of agricultural sciences,Hangzhou 310021 (china) [a] institute of Environmental Resources and Soil Fertilizer Zhejiang Academy of Agricultural Sciences Hangzhou 310021 (China)

N availability is one of the most important factors limiting crop yield *** recovery of applications of 15 N-labeled fertilizer and crop residues in a rice-wheat cropping system was determined for up to 6 consecutive growing *** crop residues from the previous season were either incorporated or removed as two different *** results showed that 16.55%-17.79% (17.17% on average) of the fertilizer N was recovered in the crop during the first growing season,suggesting that more than 80% of crop N was not directly from the N *** 15 N-labeled residues were applied,12.01% was recovered in the crop in the first growing *** average recoveries of fertilizer N and crop residue N in the soil after the first growing season were 33.46% and 85.64%,respectively.N from soil organic matter contributed approximately 83% of the N in the crop when 15 N fertilizer was applied or 88% when crop residues were *** was a larger difference in the total 15 N recovery in plant and soil between N applications in the forms of fertilizer and crop *** of crop residues following the 15 N fertilizer application did not significantly promote 15 N recovery in the crop or *** average,only additional 1.94% of N for the fertilizer-applied field or 5.97% of N for the crop residue-applied field was recovered by the crops during the 2nd and 3rd growing *** total recoveries of 15 N in crop and soil were approximately 64.38% for the fertilizer-applied field after 6 growing seasons and 79.11% for the crop residue-applied field after 5 growing *** fertilizer N appeared to be more readily available to crops than crop residue N,crop residue N replenished soil N pool,especially N from soil organic matter,much more than fertilizer ***,crop residue N was a better source for sustaining soil organic *** results suggested that the long-term effect of fertilizer or crop residues on N recovery were differen

关键词： crop residue 15 N recovery N fertilizer

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

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Science china(Life sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CHEN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 Biotechnology research institute, Chinese academy of agricultural sciences, Beijing 100081, china 2 National Laboratory of Plant Physiology and Biochemistry, College of Biological sciences, china agricultural University, Beijing 100094, china 3 College of Life sciences, Beijing Normal University, Beijing 100875, china 4 National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, china Biotechnology research institute Chinese Academy of Agricultural Sciences Beijing 100081 China National Laboratory of Plant Physiology Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China College of Life sciences Beijing Normal University Beijing 100875 China National Key Facility of Crop Gene Resources Genetic Improvement Beijing 100081 China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

Relationship between plant species diversity and patch characteristics in a marsh in the Sangjiang Plain,china—Using Yaluhe farm as an example

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Progress in Natural Science:Materials International 2007年第6期17卷 664-669页

作者： Sun Zhonglin1, Xing Fu1,2, Lu Xianguo2, Xu Kun1, Yu Lili1 and Ni Hongwei3(1. Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun 130024, china 2. Northeast institute of Geography and agricultural Ecology, Chinese academy of sciences, Changchun 130012, china 3. Natural Resources Instute of Heilongjiang academy of sciences, Harbin 150040, china) [a]Key Laboratory of Vegetation Ecology Ministry of Education Changchun China [b]Northeast institute of Geography and agricultural Ecology Chinese Academy of Sciences Changchum China [c]Natural Resources Instute of Heilongjiang academy of sciences Harbin China

Based on Quick Bird satellite image data, the present study examined small-scale herbaceous community patches in the marsh of the Sanjiang Plain and measured patch characteristics such as the area, perimeter and shape by GPS. The coenological characteristics of the patches were investigated using quadrat method, and the vascular plant species were recorded using transect method. A total of 219 plant species were recorded, which represents 12.2% of the vascular flora in Heilongjiang Province. Our results showed that species number S correlated with patch area and perimeter; richness index (R) correlated with patch perimeter; Simpson index (D) correlated with patch area and perimeter/area; Shannon-Wiener index (H′) correlated with patch area, perimeter and perimeter/area; Evenness index (Jsw) correlated with perimeter/area. Both S and R have significant difference between different size of patches. Plant diversity in the small-scale herbaceous communities tended to increase with the patch area and perimeter. We concluded that edge effects and the habitat heterogeneity of the patches are important in determining the number of species. The relative smoothness of the patch edges also affects the plant diversity of the patches. The richness of plant species in the marsh in the Sanjiang Plain is highly sensitive to the small-scale patches, which continues to play an important role in preserving plant diversity in the local area.

关键词： marsh in Sanjiang Plain plant diversity community patch habitat fragmentation small scale edge effect.

Studies on Rapid Quantitative Analysis of Bt Toxin by Using Envirologix Kits in Transgenic rice

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agricultural sciences in china 2002年第2期1卷 121-124页

作者： XIE Xiao-bo and SHU Qing-yao( Key Laboratory of Nuclear agricultural sciences , Ministry of Agriculture and Zhejiang Province ,institute of Nuclear agricultural sciences , Zhejiang University, Hangzhou 310029 National rice Improvement Center ,china National rice research institute, Hangzhou 310006) Key Laboratory of Nuclear agricultural sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029 National rice Improvement Center China National Rice Research Institute Hang Key Laboratory of Nuclear agricultural sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029

Investigations were done on the usefulness of Envirologix CrylAb/CrylAc Plate kits for quantitative analysis of Bt toxin content in transgenic rice grains. Two transgenic rice lines: Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2), transformed with a crylAb gene, and their parental variety, cv. Xiushui 11, were used as positive and negative samples. Results showed that the correlation coefficients as high as 0. 985 - 0. 998, significant at probability level of 0. 05 or 0.01, were obtained for linear regression equations by using the appended calibrators of the kits. No significant differences were detected for values of same rice sample obtained from different trials or by using different lots of the product (kit). The detectable Bt toxin content by this method could be as low as 0.5 ng/g. The Envirologix Kit could be useful for rapid quantitative detection of Bt toxin in rice grains because of its preciseness, simplicity and time saving.

关键词： Envirologix CrylAb/CrylAc plate kit Bt toxin Transgenic rice

Responses of ground-dwelling spiders to four hedgerow species on sloped agricultural fields in Southwest china

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Progress in Natural Science:Materials International 2009年第3期19卷 337-346页

作者： Yuhong Wu a, Qingnian Cai b, Chaowen Lin c, Yibing Chenc, Yuying Lid, Xu Cheng b,* a College of Resources and Environmental sciences, china agricultural University, Beijing 100193, china b College of Agriculture and Biotechnology, china agricultural University, Beijing 100193, china c institute of Soils and Fertilizers, Sichuan academy of agricultural sciences, Chengdu 610066, china d College of Life Science and Technology, Nanyang Normal University, Nanyang 473061, china [a]College of Resources and Environmental sciences China Agricultural University Beijing 100193 China [b]College of Agriculture and Biotechnology China Agricultural University Beijing 100193 China [c]Institute of Soils and Fertilizers Sichuan Academy of Agricultural Sciences Chengdu 610066 China [d]College of Life Science and Technology Nanyang Normal University Nanyang 473061 China

Spiders are important predators in agroecosystems and contribute to the control of agricultural pest populations. Habitat manage- ment such as the creation of new semi-natural habitats around or within fields enhances spider abundance and species diversity. Using pitfall traps, we investigated the effects of four hedgerow plant species, which serve as undisturbed and permanent semi-natural habitats, on ground-dwelling spider activity density (a parameter of population density and relative activity) and species richness. Samples were collected over two winter wheat and two summer maize growing seasons during 2005-2007 in trial field 1 (slope gradient of 20%) and field 2 (slope gradient of 12%) at the Ziyang Experimental Site in Sichuan Province, china. The hedgerow species evaluated were Amorpha fruticosa (field 1), Vetiveria zizanioides (field 1), Eulaliopsis binata (field 2), and Medicago sativa (field 2). Compared to control plots, hedgerow plots had significantly higher activity density and species richness of ground-dwelling spiders within strips in both fields 1 and 2 during the wheat growing season. The presence of hedgerow strips did not augment the activity density and species richness of ground-dwelling spiders within the crop fields during the wheat or maize growing seasons. The ground-dwelling spider activity density within hedgerow strips was significantly higher in the Vetiveria than in the Amorpha plots and in the Medicago than in the Eulaliopsis plots, and the species richness was significantly higher in the Vetiveria than in the Amorpha plots during the wheat season. Our results suggest that hedgerows may serve as important overwintering sites for ground-dwelling spiders during the wheat growing season. In addition, the diversification of agroecosystems by using hedgerow strips may be a viable strategy for maintaining ground-dwelling spider populations in agricultural areas. However, ground-dwelling spiders did not move into adjacent crop fields; the

关键词： Activity density Araneae Ground-dwelling Hedgerow Pitfall trapping Species richness

LAND RECLAMATION PROCESS IN NORTHEAST china SINCE 1900

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Chinese Geographical Science 2003年第2期13卷 119-123页

作者： ZHANG Bai, CUI Hai-shan, YU Lei, HE Yan-fen (Northeast institute of Geography and agricultural Ecology, Chinese academy of sciences, Changchun 130012,P.R.china) Northeast institute of Geography and agricultural Ecology Chinese Academy of Sciences Changchun P. R. China

Northeast china is an important agricultural region in china. The NortheastPlain is the largest plain in the country and one of the three famous black soil zones in the *** of fertility of the soil, however, large-scale agricultural development mainly took placesince 1900. The land exploitation and utilization has been fast and intensive in this region sincethen and change in the land-cover process has been remarkable. Both national and internationalresearchers on land use and land cover are paying more attention to these processes in this regionand their implication for local ecological environment. This article discusses the land reclamationprocesses and their main driving forces in Northeast china since 1900. According to the research,the 20th century was the most important period for land reclamation in Northeast china. In theseyears the rate, intensity and quantity of land reclamation have been staggering, and more than 100000km^2 of land was converted into farmland. This magnitude of land reclamation inland is unique inthe world. research on the land reclamation of Northeast china can provide some data on the effectof human activity on environment. As in many other places in the world, the primary driving force ofreclamation in Northeast china was the increasing pressure of population. In the 20th century thepopulation increased from 10 x 10~6 to 110 x 10~6 in Northeast china and from 400 x 10~6 to 1. 3 x10^9 in china. Population pressure is thus the most important driving force for land reclamation.

关键词： land reclamation process northeast china 20th century

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香蕉作物施用肥料增效剂的效应

土壤肥料 2003年第4期 36-39页

作者：陈琼贤彭志平广东省农科院蔬菜研究所广州510640 广东省农科院土壤肥料研究所广州510640

以香蕉和粉蕉为试验材料,研究施用肥料增效剂的效果。结果表明:施用肥料增效剂能极显著增加蕉果产量,增产量为7 85t/hm2～22 15t/hm2,相对增产19 6%～57 8%,收益增加7704元/hm2～23150元/hm2,每公斤肥料增效剂增值8 87元～14 82元。肥... 详细信息

以香蕉和粉蕉为试验材料,研究施用肥料增效剂的效果。结果表明:施用肥料增效剂能极显著增加蕉果产量,增产量为7 85t/hm2～22 15t/hm2,相对增产19 6%～57 8%,收益增加7704元/hm2～23150元/hm2,每公斤肥料增效剂增值8 87元～14 82元。肥料增效剂的适宜用量为1500kg/hm2,施用肥料增效剂能加快出叶速度,提早现蕾28～60天,增强蕉株抗寒力,明显增加蕉叶N、P2O5、K2O、MgO、S、Zn和B的养分含量。

关键词：香蕉肥料增效剂肥效产量品质施用量

Analysis of Resistant Spectrum to rice Blast in Transgenic rice Lines Introduced Lysozyme Gene from T4 Phage

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agricultural sciences in china 2003年第3期2卷 273-279页

作者： XU Ming-hui, LI Cheng-yun , LI Jin-bin, TAN Xue-lin, TIAN Wen-zhong and TANG Zuo-shuninstitute of Biotechnology , Yunnan academy of agricultural sciences , Kunming 650223 , P. R. china institute of Plant Protection , Yunnan academy of agricultural sciences , Kunming 650205 , P. R. china rice research institute, Yunnan agricultural University, Kunming 650201 , P. R. china institute ofGenetics and Development Biology , Chinese academy of sciences , Beijing 100101 , P. R. china) institute of Biotechnology Yunnan Academy of Agricultural Sciences Kunming 650223 P. R. China institute of Plant Protection Yunnan Academy of Agricultural Sciences Kunming 650205 P. R. China rice research institute Yunnan Agricultural University Kunming 650201 P. R. China institute of Genetics and Development Biology Chinese Academy of Sciences Beijing 100101 P. R. China

The receptor cultivar Nan29 and thirty-six T5 rice lines derived from ten T0 generation transgen-ic plants harboring lysozyme gene were challenged in the greenhouse by inoculating 63 isolates belonging to 48 races of Magnaporthe grisea from Yunnan Province. The transgenic rice lines exhibited resistance to more than 72% of isolates inoculated in this experiment, and 38.1% (24 isolates) of them could infect the receptor cultivar Nan29. The results indicated that the transgenic rice lines possessed wide-spectrum resistance against various rice blast races and the resistant spectrum of rice lines were different although some lines derived from same T0 plant. The transgenic rice lines exhibited also high resistance to leaf and neck blast in the disease field evaluation, but not all of resistant lines against leaf blast were resistant to neck blast.

关键词： rice Lysozyme gene rice blast Resistant spectrum