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Taurine inhibits ischemia/reperfusion-induced compartment syndrome in rabbits

Acta Pharmacologica Sinica 2005年第7期26卷 821-827页

作者： Ji-xian WANG~(2,3) Yan LI~2 Li-ke ZHANG~4 Jing ZHAO~5 Yong-zheng PANG~(2,3) Chao-shu TANG~(3,5) Jing ZHANG~(2,3,6) 1 Institute of cardiovascular Disease Research,The First Hospital of Peking University,beijing 100034,china 3 key laboratory of molecular cardiovascular science,ministry of education,beijing 100083,china 4 Department of Pathophysiology,Capital Institute of Medicine,beijing 100054,china 5 Department of Physiology Health Center of Medical sciences,Peking University,beijing 100083,china Institute of cardiovascular Disease Research The First Hospital of Peking University Beijing China key laboratory of molecular cardiovascular science Ministry of Education Beijing China Department of Pathophysiology Capital Institute of Medicine Beijing China Department of Physiology Health Center of Medical Sciences Peking University Beijing China

Aim:To investigate effects of taurine on ischemia/reperfusion(I/R)-induced com-partment syndrome in rabbit hind ***:Rabbits underwent femoralartery occlusion after ligation of branches from terminal aorta to femoral *** a 7-h ischemia,reperfusion was established with the use of heparinizedpolyethylene *** received taurine(1 g/kg)or normal saline(control)by iv infusion 10 min before shunt *** reperfusion,anterior com-partment pressure(ACP)was monitored continuously in the left lower *** muscle triphenyltetrazolium chloride(TTC)level,taurine contentand myeloperoxidase activity were *** stress was induced in thein vitro gastrocnemius muscle slices by free radical generating systems(FRGS),and the malondialdehyde content was measured in presence or absence of ***:After 7 h of ischemia,none of the parameters that we measured weredifferent from those before ischemia,except that TTC reduction decreased by80%.In the control group,after 2 h of reperfusion,ACP increased 4.5-fold,andgastrocnemius muscle taurine content was reduced by 33%.In taurine-treatedanimals,at 2 h reperfusion,the mean arterial blood pressure and heart rate wereincreased,by 6% and 10%.ACP decreased by 39%,muscle edema decreased by16%,TTC reduction increased by 150%,and lactate dehydrogenase decreased by36% compared to control *** and muscle taurine content increased by70% and 88%,*** the taurine-treated group,at 2 h repeffusion,plasmamalondialdehyde and conjugated diene content were decreased by 38% and 23%,respectively,and muscle malondialdehyde and conjugated diene content decreasedby 22% and 30%,respectively compared to the control *** 2 h reperfusion,myeloperoxidase activity was increased 3.5-fold in control *** the in vitrostudy,taurine decreased malondialdehyde content in muscle slices incubatedwith hypochlorous acid in a dose-dependent manner,but there was no changewhen incubated

关键词： taurine compartment syndromes reperfu sion injury

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Calcitonin gene-related peptide inhibits interleukin-1β-induced interleukin-8 secretion in human type Ⅱ alveolar epithelial cells

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Acta Pharmacologica Sinica 2006年第10期27卷 1340-1345页

作者： Wen-jing LI Teng-ke WANG Xian WANG~2 Department of Physiology,School of Basic Medical sciences,Peking University,key laboratory of molecular cardiovascular science,ministry of education,beijing 100083,china Department of Physiology School of Basic Medical Sciences Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing China

Aim:Our previous data have shown that type Ⅱ alveolar epithelial(AEⅡ)cellsexpress neuropeptide calcitonin gene-related peptide(CGRP),and that pro-in-flammatory factor interleukin 1-β(IL-1β)induces CGRP secretion in the A549 hu-man AEⅡ cell *** the present study,we investigated the effect of endogenousand exogenous CGRP on IL-1β-induced chemokine interleukin-8(IL-8)***:We used enzyme-linked immunosorbent assay(ELISA)and RT-PCR todetect IL-8 protein and mRNA levels,respectively,siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene,respectively,and chemiluminescence assay was used to detect reactive oxygenspecies(ROS)***:CGRP-1 receptor antagonist hCGRP8-37(0.1-1nmol·L-1)greatly amplified IL-1β-induced IL-8 *** inhibition of CGRPexpression by siRNA significantly increased IL-8 secretion upon IL-1β ***,cell clones stably transfected with CGRP showed significantly inhibitedmRNA and protein levels of IL-8 induced by 1L-1β.Conclusion:These data implythat AEⅡ cell-derived CGRP suppress IL-1β-induced IL-8 secretion in an autocrine/paracrine *** investigation showed that CGRP attenuated IL-1β-arousedROS formation,which is an early indication of pro-inflammatory factor signaling.

关键词： type Ⅱ alveolar epithelial cells interleukinl interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species

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Overexpression of heat-shock protein 20 in rat heart myogenic cells confers protection against simulated ischemia/reperfusion injury

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Acta Pharmacologica Sinica 2005年第9期26卷 1076-1080页

作者： Yan-hui ZHU Xian WANG~2 Department of Physiology and Pathophysiology,Basic Medical College,key laboratory of molecular cardiovascular Biology,ministry of education,Peking University,beijing 100083,china Department of Physiology and Pathophysiology Basic Medical College Key Laboratory of Molecular Cardiovascular Biology Ministry of Education Peking University Beijing China

Aim:To explore whether overexpression of the small heat shock protein HSP20 inrat cardiomyocytes protects against simulated ischemia/reperfusion(SI/R)***:Recombinant adenovirus expressing HSP20 was used to infect rat H9c2cardiomyocytes at high efficiency,as assessed by green fluorescent protein.H9c2cells were subjected to SI/R stress;survival was estimated through assessment oflactate dehydrogenase and cell apoptosis through caspase-3 ***:Overexpression of HSP20 decreased lactate dehydrogenase release by 21.5% andcaspase-3 activity by 58.8%.Pretreatment with the protein kinase C inhibitor Ro-31-8220(0.1 μmol/L)for 30 min before SI/R canceled the protective effect of *** selective mitochondrial K+awP channel inhibitor 5-hydroxydecanoate(100 μmol/L)had a similar ***,the non-selective K~+ATPchannel inhibitorglibenclamide(100 μmol/L)had no significant ***:These dataindicate that the protective effect of HSP20 in vitro is primarily due to reducednecrotic and apoptotic death of cardiomyocytes,possibly via the protein kinaseC/mitochondrial K~+ATP pathway.

关键词： reperfusion injury adenovirus apoptosis necrosis heat-shock proteins

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Gene transfer of heat-shock protein 20 protects against ischemia/reperfusion injury in rat hearts

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Acta Pharmacologica Sinica 2005年第10期26卷 1193-1200页

作者： Yan-hui ZHU, Tie-min MA, Xian WANG~2 Department of Physiology and Pathophysiology, Basic Medical College, key laboratory of molecular cardiovascular Biology, ministry of education, Peking University, beijing 100083, china Department of Physiology and Pathophysiology Basic Medical College Key Laboratory of Molecular Cardiovascular Biology Ministry of Education Peking University Beijing China

Aim: To explore whether overexpression of HSP20 in the myocardium could protect against ischemia/reperfusion injury in rats. Methods: Rat hearts were injected with vector, recombinant adenovirus encoding green fluorescent protein(***) or recombinant adenovirus encoding wild-type HSP20 (***20) in the left ventricle. Four days later, hearts were removed and expression of HSP20was measured in the left ventricle. Subsets of animals in the vector-, ***-, ***20-treated groups were subjected to 20-rain ischemia and 120-rainreperfusion. Myocardial injury was evaluated by infarct size and level of serumcardiac troponin T and creatine phosphokinase. Apoptosis of cardiomyocyteswas determined by TUNEL staining. Cardiac function was evaluated by hemodyamic indexes. Results: Infarct size and serum cardiac troponin T and creatinephosphokinase levels were significantly reduced in ***20-treated hearts compared with vector-and ***-treated hearts. The ratio of TUNEL-positivecardiomyocytes to total number of cardiomyocytes in the ***20 group wassignificantly reduced as compared with the vector and *** groups. Left ventricular end systolic pressure, and maximal rate of pressure increase (+dp/dtmax)and decrease (-dp/dtmin) values were increased significantly, while left ventricularend diastolic pressure was decreased significantly in ***20-treated heartscompared with vector- and ***-treated hearts. Conclusion: These data indicate that the cardioprotective effects of HSP20 may contribute to the reduction ofmyocardial necrosis and apoptosis in ischemia/reperfusion injury in rats.

关键词： reperfusion injury adenovirus apoptosis myocardial infarction heat-shock proteins

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Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats

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Acta Pharmacologica Sinica 2006年第3期27卷 299-306页

作者： Sheng-ying WU~(2,6) Chun-shui PAN~3 Bin GENG~3 Jing ZHAO~3 Fang YU~3 Yong-zheng PANG~3 Chao-shu TANG~4 Yong-fen QI~(3,4,5) ~2Institute of cardiovascular Disease,Peking University First Hospital.beijing 100034 ~3Department of Physiology and Pathophysiology,Peking University Health science Center,beijing 100083,china,~4key laboratory of molecular cardiovascular science,ministry of education,beijing 100083,china Institute of cardiovascular Disease Peking University First Hospital Beijing China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing China key laboratory of molecular cardiovascular science Ministry of Education Beijing China

Aim:To investigate the role of the endogenous cystathionine γ-synthase(CSE)/hydrogen sulfide(H2S)pathway in vascular calcification in ***:A ratvascular calcification model was established by administration of vitamin D3 plusnicotine(VDN).The amount of CSE and osteopontin(OPN)mRNA was deter-mined by using semi-quantitative reverse-transcription polymerase chain *** calcium content,45Ca2+accumulation and alkaline phosphatase(ALP)activitywere measured.H,S production and CSE activity were ***:yonKossa staining produced strong positive black/brown staining in areas amongthe elastic fibers of the medial layer in the calcified *** calcium content,45Ca2+accumulation and ALP activity in calcified arteries increased by 6.77-,1.42-,and 1.87-fold,respectively,compared with *** expression of the OPNgene was upregulated(P34.8%,40.75% and 63.5% lowerin the low-dosage NariS group than in the VDN group,respectively(P3.9%,37.8 % and 46.2% lower in the aorta in the high-dosage NariS group than in theVDN group,respectively(P<0.01).The expression of the OPN gene ***:The production of H,S,and CSE activity were de-creased and CSE gene expression was downregulated in rats with vascularcalcification.H2S can ameliorate vascular calcification,suggesting that the H2S/CSE pathway plays a regulatory role in the pathogenesis of vascular calcification.

关键词： hydrogen sulfide cystathionine-lyase vascular calcification mRNA

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Magnetism induced by nonmagnetic dopants in zinc-blende SiC: First-principle calculations

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science china(Physics,Mechanics & Astronomy) 2010年第1期53卷 1-10页

作者： LIU ZhaoQing & NI Jun Department of Physics and key laboratory of Atomic and molecular Nanoscience (ministry of education), Tsinghua University, beijing 100084, china Department of Physics and key laboratory of Atomic and molecular Nanoscience (ministry of education) Tsinghua University Beijing China

Magnetism induced by the nonmagnetic dopants in the zinc-blende SiC (3C-SiC) is investigated by first-principle calculations. The atoms of the first 20 elements in the periodic table except inert gas are used to replace either Si or C atoms as dopants. We find that some nonmagnetic substitutional dopants (mainly the Group IA, Group IIA, Group IIIB, and Group VIIB elements) prefer the spin-polarized ground states with local magnetic moments. In general, the condition for obtaining the local magnetic moments and the magnetic ground state requires that the dopants are p-type and have large electronegativity difference from the neighboring host atoms. The magnetic moments can be tuned over a range between 1 μ B and 3 μ B by doping with the nonmagnetic elements. The nearest-neighbor exchange couplings J 0 between the local magnetic moments are quite large and the codoping method is proposed to increase the dopant concentration. These imply that the nonmagnetic doping in SiC may exhibit collective magnetism. Moreover, the Group IIA Mg and Ca atoms substituting the preferred Si atoms favor the ferromagnetic ground states with the half-metallic electronic properties, which suggests that Mg or Ca substitutional doping on the Si sites in SiC could be a potential route to fabricating the diluted magnetic semiconductors.

关键词： nonmagnetic dopant magnetism SiC diluted magnetic semiconductor first-principle calculation

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Crosstalk between signaling pathways of adrenoreceptors and signal transducers and activators of transcription 3(STAT3)in heart

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Acta Pharmacologica Sinica 2007年第2期28卷 153-161+163-165页

作者： Kai-zheng GONG Hui ZHANG Jian-hai DU You-yi ZHANG~2 Institute of Vascular Medicine,Peking University Third Hospital,key laboratory of molecular cardiovascular sciences,ministry of education beijing 100083,china Institute of Vascular Medicine Peking University Third Hospital Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China

Recently,there have been important advancements in our understanding of thesignaling mechanisms of adrenoreceptors(AR)and signal transducers and acti-vators of transcription 3(STAT3).While their crucial roles in the pathologicalprocesses of the heart are well established,accumulating evidence suggests thereis a complex pattern of crosstalk between these 2 signaling ***,the potential for crosstalk occurs at multiple levels in each signaling cascade andinvolves receptor transactivation,G proteins,small GTPases,cyclic adenosine 3’,5’-monophosphate/protein kinase A,protein kinase C,scaffold/adaptor proteins,protein tyrosine kinases,and mitogen-activated protein *** addition,post-translational modification(eg acetylation)of STAT3 may provide a link betweenSTAT3 and AR *** particular,crosstalk between these 2 systems in theheart would appear to be dependent upon the species/tissue studied,develop-mental stage,and eliciting *** at least partly accounts for the epige-netic effects on biological function that is mediated by the 2 signaling *** of these mechanisms will provide new targets in the development ofnovel clinical strategies for heart disorders.

关键词： receptors adrenergic signal transduction STAT3 transcription factor heart

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Cardioprotective effects of ghrelin and des-octanoyl ghrelin on myocardial injury induced by isoproterenol in rats

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Acta Pharmacologica Sinica 2006年第5期27卷 527-535页

作者： LLian LI~2 Li-ke ZHANG~5 Yong-zheng PANG~(2.4) Chun-shui PAN~(3.4) Yong-fen QI~(3.4) Li CHEN~4 Xian WANG~(3,4) Chao-shu TANG~(3,4) Jing ZHANG~(2,4,6) ~2Institute of cardiovascular Research,Peking University First Hospital,beijing 100034,china:~3Department of Physiology and Pathophysiology,Peking University Health science Center,beijing 100083,china ~4key laboratory of the ministry of education on molecular Cardiology,beijing 100083,china:~5Department of Pathophysiology,Capital University of Medicine,beijing 100054,china Institute of cardiovascular Research Peking University First Hospital Beijing China Department of Pathophysiology Capital University of Medicine Beijing China key laboratory of the ministry of education on molecular Cardiology Beijing China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing China

Aim:To determine the cardioprotective action of ghrelin and des-octanoyl ghrelinin rats with isoproterenol-induced myocardial ***:Rats were subcu-taneously injected with isoproterenol （ISO;20,10,and 5 mg/kg） on d 1,2 and 3,respectively,and then 3 mg/kg for the next 7d with or without ghrelin or des-octanoyl-ghrelin （100μg/kg,twice daily）.Plasma ghrelin and growth hormonelevels were assayed using radioimmunoassay *** hormone secreta-gogue receptor （GHSR） and ghrelin mRNA were determined using *** binding capacity and the affinity for [3H]ghrelin were determined by re-ceptor binding ***:Compared with controls,ISO-treated rats showedsevere myocardial injury,cardiomegaly,infarction-like necrosis and massive fi-brosis with increases in irradiated-ghrelin （ir-ghrelin） content in plasma by 67%and myocardia by 66% and in the mRNA level in the myocardia by 93% （P3H]ghrelin for myocardial sarcolemma was higherin ISO-treated rats than in *** administration improved cardiac func-tion and ameliorated cardiomegaly and attenuated myocardial lipid peroxidationinjury and relieved cardiac fibrosis as compared with ISO treatment ***-istration of des-octanoyl ghrelin effectively antagonized ISO-induced myocardialinjury and improved all parameters ***,the therapeutic effect ofdes-octanoyl ghrelin was significantly weaker than that of *** plasmagrowth hormone level increased markedly,by 1.5-fold （P<0.01）,with ghrelinadministration as compared with that in controls,but was unaltered in the des-octanoyl ghrelin ***:Myocardial ghrelin and GHSR were up-regulated during ISO-induced myocardial *** protective effect of ghrelinagainst ISO-induced cardiac function injury and fibrosis was more potent thanthat of des-octanoyl ghrelin,which suggests that ghrelin could be an endog

关键词： ghrelin des-octanoyl ghrelin myocardium fibrosis isoproterenol

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Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation

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Acta Pharmacologica Sinica 2007年第3期28卷 359-366页

作者： Shu-yi WANG~2 Yao SONG~2 Ming XU~2 Qi-hua HE~3 Qi-de HAN~2 You-yi ZHANG~(2,4)2 Institute of Vascular Medicine,Peking University Third Hospital and key laboratory of molecular cardiovascular sciences,ministry of education,beijing 100083,china 2 Center of Medical Analysis,Peking University,Health science Center,beijing 100083,china Institute of Vascular Medicine Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China Center of Medical Analysis Peking University Health Science Center Beijing China

Aim:To examine the subcellular distribution of the 3 α1-adrenoceptor（α1-AR）subtypes and their internalization and trafficking upon agonist stimulation in hu-man embryonic kidney 293A ***:Confocal real-time imaging,enzymelinked immunosorbent assay（ELISA）and whole cell[3H]-prazosin binding assaywere applied to detect the distribution and localization of the 3 α1B-AR ***:α1A-AR was found both on the cell surface and in the cytoplasm;α1B-AR,however,was predominantly detected on the cell surface,while α1D-AR wasdetected mainly in the intracellular *** stimulation withphenylephrine,localization changes were detected by confocal microscopy forα1A-and α1B-AR, but the localization of α1D-AR were *** promoted a more rapid internalization of α1B-AR than α1A-AR.α1D-ARinternalization was detected only by *** cell[3H]-prazosin bindingassay showed that α1A-AR functional receptors were detected both on the cellsurface and in the cytoplasm;α1B-AR,however,were detected predominantly onthe cell surface,while α1D-AR were detected mainly in intracellular *** stimulation promoted internalization of α1A-and α***:Phenylephrine stimulation induced changes in the localization of the3α1-AR.

关键词： adrenoceptor agonist stimulation confocal image enzyme-linked immunosorbent assay [~3H]-prazosin binding assay internalization subcellular distribution subtype

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Cloning and characterization of cotton heteromeric acetyl-CoA carboxylase genes

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Progress in Natural science:Materials International 2007年第12期17卷 1412-1418页

作者： Qiao Zhixin and Liu Jinyuan(laboratory of molecular Biology and Protein science laboratory of the ministry of education, Department of Biological sciences and Biotechnology, Tsinghua University, beijing 100084, china) laboratory of molecular Biology and Protein science Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Heteromeric acetyl-coenzyme A (CoA) carboxylase (ACCase) catalyzes the formation of malonyl-CoA from acetyl-CoA. It plays an essential role in fatty acid synthesis in prokaryotes and most of plants. The heteromeric ACCase is composed of four subunits: biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and α- and β-subunits of carboxyltransferase (α- and β-CT). In this study, we cloned five novel genes encoding the subunits of heteromeric ACCase (one BC, BCCP and β-CT, and two α-CTs) from cotton (Gossypium hirsutum cv. zhongmian 35) by RACE-PCR. The deduced amino acid sequence of these cDNAs shares high similarity with other reported heteromeric ACCase subunits. The phylogenetic analysis indicated that the different subunits of heteromeric ACCase were grouped in a similar pattern. Southern blot analysis revealed the milti-copy patterns of these heteromeric ACCase genes in cotton genome. Semi-quantitative RT-PCR demonstrated that heteromeric ACCase genes were constitutively expressed in all of the cotton tissues, but the transcripts accumulated at a relatively low level in roots. To our knowledge, this is the first report about characterization of the heteromeric ACCase genes in cotton.

关键词： heteromeric ACCase cotton gene cloning fatty acid synthesis.

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