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中日及泛亚太地区菌物学论坛

作者： Chengshu Wang institute of plant physiology and ecology.shanghai institutes for biological sciences.CAS shanghai china

＜正＞Metarhizium anisopliae is an insect fungus of considerable metabolic and ecological *** kills insects by cuticle penetration,colonization and multiplication in *** surface adhesion,we found that *** uses two dif...

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Molecular mechanism of G-protein-regulated chloroplast development

Molecular mechanism of G-protein-regulated chloroplast devel...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Jirong Huang National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china

Heterotrimeric G-proteins have been demonstrated to play an important role in many physiological processes in *** first evidence for the involvement of G-proteins in chloroplast development came from pharmaceutical studies on tomato hypocotyl cells of the aurea mutant that is defective in phytochrome A(Neuhaus et al.,1993).It

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Role of oxyR from Sinorhizobium meliloti in Regulating the Expression of Catalases

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 421-428页

作者： Li EUO~(1.3) Ming-Sheng QI~(1.3) Shi-Yi YAO~2 Hai-Ping CHENG~2 Jia-Bi ZHU~1 Guan-Qiao YU~1 1 National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences. shanghai 2.0032. china 2 biological sciences.Department, Lehman College, the City University of New York, Bronx, NY 10468. USA, 3 Graduate School of the chinese academy of sciences. shanghai 2.0032. china NationalLaboratoryofplantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologi

The process of symbiotic nitrogen fixation results in the generation of reactive oxygen speciessuch as the superoxide anion（O2-）and hydrogen peroxide（H2O2）.The response of rhizobia to these toxicoxygen species is an important factor in nodulation and nitrogen *** Sinorhizobium meliloti,oneoxyR homologue and three catalase genes,katA,katB,and katC were detected by sequence *** gene is located next to and divergently from katA on the *** investigate the possible rolesof oxyR in regulating the expression of catalases at the transcriptional level in ***,an insertion mutantof this gene was *** mutant was more sensitive and less adaptive to H2O2than the wild typestrain,and total catalase/peroxidase activity was reduced approximately fourfold with the OxyR mutationrelative to *** activities of KatA and KatB and the expression of katA::lacZ and katB::lacZ promoterfusions were increased in the mutant strain compared with the parental strain grown in the absence of H2O2,indicating that katA and katB are repressed by ***,when exposed to H2O2,katA expressionwas also increased in both *** and Escherichia *** exposed to H2O2,OxyR is convertedfrom a reduced to an oxidized form in *** concluded that the reduced form of OxyR functions as arepressor of katA and katB ***,in the presence of H2O2,reduced OxyR is converted to theoxidized form of OxyR that then results in increased katA *** further showed that oxyR expressionis autoregulated via negative feedback.

关键词： Sinorhizobium meliloti OxyR catalase katA H2O2

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新世纪微生物学者的一项重要任务——未培养微生物的分离培养

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生物工程学报 2004年第5期20卷 641-645页

作者：焦瑞身中国科学院上海生命科学研究院植物生理生态研究所上海200032

扼要介绍近年来有关分离培养未培养的微生物 (Unculturablemicroorganisms)、扩大生态环境微生物多样性的认识等方面取得的重要进展。首先 ,是采用非常规的 ,甚至认为有毒性的电子传递物质 ,获得了多种新生理型(physiotypes)的纯种微生... 详细信息

扼要介绍近年来有关分离培养未培养的微生物 (Unculturablemicroorganisms)、扩大生态环境微生物多样性的认识等方面取得的重要进展。首先 ,是采用非常规的 ,甚至认为有毒性的电子传递物质 ,获得了多种新生理型(physiotypes)的纯种微生物。另外 ,在多种生态域中分离出非同寻常的微生物 ,扩大了这些生境微生物的多样性的视野 ,如普遍存在于大洋的浮游细菌SAR11类群 ,其微小菌体呈半月形 ,在海水中细菌浓度可达 (10 5～ 10 6 ) mL ,基因组估计为 1 5 4Mb。更值得注意的是从极端高温环境分离的嗜热纳米古细菌属 (Nanoarchaeota) ,其基因组仅有5 0 0kb ,是已知原核生物的最小者。应重视的是在分离这些多样性微生物的过程中所发展的新型培养技术 ,如微滴胶囊化法和扩散小室法都是具有革命性的方法 ,既有通用性 ,又无需昂贵设备。

关键词：微生物学者微生物多样性生态环境生理型嗜热纳米古细菌

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Control of Rice Grain Filling and Duplication and Independent Selection of the Cell Wall Invertase Genes

Control of Rice Grain Filling and Duplication and Independen...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Ertao Wang, Qun Li, Zuhua He* institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. shanghai 2.0032. china.

＜正＞Rice grain-filling, a process controlling the weight of each grain after pollination, has been a bottleneck in

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Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules

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Cell Research 2006年第10期16卷 818-829页

作者： Zi Ying Gong~1 Zhi Shui He~1 Jia Bi Zhu~1 Guan Qiao Yu~1 Hua Song Zou~1 ~1National Laboratory of plant Molecular Genetics,shanghai institute of plant physiology and ecology.shanghai institutes.for biological sciences.chinese academy of sciences.shanghai 2.0032.china NationalLaboratoryofplantMolecularGenetics ShanghaiInstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200032China

Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in *** understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa,the cDNA-AFLP technique wasemployed to study the changes in gene expression in nifA mutant *** the approximately 3,000 transcript-derived fragments,37 had differential expression *** expression levels were subsequently confirmed by reverseNorthern blot and RT-polymerase chain *** analyses revealed that 21 cDNA fragments corresponded togenes involved in signal communication,protein degradation,nutrient metabolism,cell growth and development.

关键词： cDNA-AFLP gene expression nodule Sinorhizobium meliloti nifA

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A proteomic approach to analyzing responses of callus cells and root tip cells of Arabidopsis thaliana to altered gravity

A proteomic approach to analyzing responses of callus cells ...

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2006年拟南芥研究学术研讨会

作者： Huiqiong Zheng, Hui Wang institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. 300 Fenglin Road, shanghai 2.0032. china

＜正＞Gravity has a profound influence on plant growth and development. Removal the influence of gravitational acceleration by spaceflight may lead to a wide range of cellular changes in plant. Whole seedling, that germinated and grown on clinostats （simulated weightlessness）, showed an absence of gravitropism. At the cellular level, clinostat treatment has specific effects on plant cells, such as alterations in cell wall composition, increased production of heat-soluble proteins, changes in cellular energy metabolism, facilitating a uniform distribution of plastids （amyloplasts）, and an increase in the number and volume of nucleoli.

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BP regulates root skewing in gravity-dependent processes

BP regulates root skewing in gravity-dependent processes

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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Bin Qi,Huiqiong Zheng institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Road, shanghai,2.0032.china

plant knotted-like homeobox(KNOX) genes,which encode homeodomain transcription factors and play a key role in maintaining a pluripotent cell population in the shoot apical meristem,have been extensive studied,but their function in root is still *** this study,we show that the BREVIPEDECELLUS(BP) gene,which belongs to KNOXI Class family,is specifically involved in the regulation movements of Arabidopsis *** of bp-1 mutant grown on inclined plate exhibit right-handed axial twisting and the epidermal cells in its elongation zone appeared left-handed *** addition,the root of bp mutant also reduced gravitropic response and displayed a curling phonetype. Further tests show the pattern of auxin-induced gene expression,auxin response,basipetal auxin transport PIN2-green fluorescent protein are altered in bp ***,addition of auxin transport inhibitor TIBA abolishes root-continued skewing of bp mutant or the loss PIN2 function abolishes root curling of bp mutant *** results demonstrate that BP could play an important role in regulating root skewing movement with auxin mediation in gravity-dependent processes.

关键词： Arabidopsis skewing gravitropism KNAT1

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Transcriptional, post-transcriptional and post-translational gene regulations during leaf polarity formation

Transcriptional, post-transcriptional and post-translational...

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2006年拟南芥研究学术研讨会

作者： Hai Huang National Laboratory of plant Molecular Genetics, shanghai institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. shanghai 2.0032. china

＜正＞Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shoot apical meristem （SAM）. Recent studies on leaf morphogenesis have led to significant progress towards understanding the genetic and molecular control of leaf polarity establishment. In particular, several transcription factors have been found to play critical roles in promoting adaxial or

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Genome-wide Linkage Disequilibrium and Recombination Map in Rice

Genome-wide Linkage Disequilibrium and Recombination Map in ...

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第十二届全国植物基因组学大会

作者： Kunyan Liu,Bin Han~* National Center For Gene Research,institute of plant physiology and ecology.shanghai institutes For biological sciences.chinese academy of sciences.shanghai 200233,china

Linkage disequilibrium(LD),the nonrandom association between alleles at different loci,is a noticeable feature of the organization of genetic variations in natural *** knowledge of LD facilitates the determination of marker density and gives the clues of the resolution of association ***,such critical information in rice is still ***,we explored the pattern of linkage disequilibrium in rice using resequencing data of～600 diverse rice *** expected,the background of LD measured by the

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