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Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2) Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 State Key Laboratory for Infectious Disease Prevention and Control,National institute for Viral Disease Control and Prevention,Chinese center for Disease Control and Prevention,beijing 100052,china 2 School of Medicine,Xi'an Jiaotong university,Xi'an 710061,china 3 National Laboratory of Medical Molecular Biology,institute of Basic Medical science,Chinese Academy of Medical sciences and peking Union Medical College,beijing 100005,china 4 College of science and Veterinary Medicine,Northwest Agriculture and Forest university,Yangling 712100,china State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052 China School of Medicine Xi'an Jiaotong UniversityXi'an 710061 China National Laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005 China College of science and Veterinary Medicine Northwest Agriculture and Forest UniversityYangling 712100 China

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 23-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

Inhibition of angiotensin Ⅱ and blockade of endothelin receptors reduce arterial calcification in rats

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Journal of Geriatric Cardiology 2004年第2期1卷 108-113页

作者： Juxiang LI~1 Shengying WU~1 Chunshui PAN~2 Yongfen QI~2 Bin GENG~1 Xiuhna LIU~~(1,2) Chaoshu TANG~1 1 Department of Physiology and Pathophysiology,peking university health science center,beijing 100083,china 2 Insitiute of cardiovascular Research,peking university First Hospital,beijing 100034,china Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China Insitiute of cardiovascular Research Peking University First Hospital Beijing 100034 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China Insitiute of cardiovascular Research Peking University First Hospital Beijing 100034 China

Objective To examine whether the two vascular paracrine/autocrine factors,angiotensin Ⅱ(Ang Ⅱ)and endothelin,participate in the pathogenesis of arterial *** Nicotine and vitamin D_3 treated rats were *** calcification was confirmed by using Von Kossa staining,measurement of calcium content, ^(45)Ca^(2+)uptake assay and alkaline phosphatase(ALP)*** plasma and vascular Ang Ⅱ and endothelin levels were measured by using *** and endothehn mRNA levels were determined by RT- *** The arterial calcium content,^(45)Ca^(2+)uptake and ALP activity were increased in calcification groups compared with control(P3.)

关键词： aorta angiotensin Ⅱ endothelin-1 calcification

Integrative inversion of land surface component temperature

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science china Earth sciences 2005年第11期48卷 2011-2019页

作者： FAN Wenjie1 & XU Xiru1,2 1. institute of Remote Sensing and Geographical Information System, peking university, beijing 100871, china 2. center of Remote Sensing and GIS of beijing Normal university, beijing 100875, china institute of Remote Sensing and Geographical Information System Peking University Beijing China center of Remote Sensing and GIS of beijing Normal university Beijing China

In this paper, the row winter wheat was selected as the example to study the com-ponent temperature inversion method of land surface target in detail. The result showed that the structural pattern of row crop can affect the inversion precision of component temperature evi-dently. Choosing appropriate structural pattern of row crop can improve the inversion precision significantly. The iterative method combining inverse matrix was a stable method that was fit for inversing component temperature of land surface target. The result of simulation and field ex-periment showed that the integrative method could remarkably improve the inversion accuracy of the lighted soil surface temperature and the top layer canopy temperature, and enhance inver-sion stability of components temperature. Just two parameters were sufficient for accurate at-mospheric correction of multi-angle and multi-spectral thermal infrared data: atmospheric trans-mittance and the atmospheric upwelling radiance. If the atmospheric parameters and component temperature can be inversed synchronously, the really and truly accurate atmospheric correction can be achieved. The validation using ATSRII data showed that the method was useful.

关键词： components temperature, inverse pattern, atmospheric correction

Response of remotely sensed Normalized Difference Water Deviation Index to the 2006 Drought of eastern Sichuan Basin

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science china Earth sciences 2008年第5期51卷 748-758页

作者： ZHANG WenJiang1, LU QiFeng2, GAO ZhiQiang3 & PENG Jian4 1 School of Water Resource and Hydropower, Sichuan university, Chengdu 610065, china 2 National Satellite Meteorological center, china Meteorological Administration, beijing 100081, china 3 institute of Geographic sciences and Natural Resources Research, Chinese Academy of sciences, beijing 100101, china 4 School of Environmental sciences, peking university, beijing 100871, china School of Water Resource and Hydropower Sichuan University Chengdu China National Statellite Meteorological center China Meteorological Administration Beijing China institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences Beijing China School of Environmental sciences Peking University Beijing China

One of the most serious droughts in last century occurred in eastern Sichuan Basin in the summer of 2006 (hereinafter called the Drought). The response of Moderate Resolution Imaging Spectroradiometer (MODIS, boarding on NASA satellites of Terra and Aqua) to the Drought was analyzed in order to reach one practicable monitoring solution for regional soil moisture. Temporal process and spatial extension of the Drought were firstly estimated with ground meteorological and hydrological observations. Then, for the whole region of Sichuan and Chongqing, the remotely sensed Normalized Difference Water In- dex (NDWI) for the summers of 2001―2006 were calculated based on 8-day composite MODIS products, which were further used to construct a new water index (Normalized Difference Water Deviation Index, NDWDI) to examine the sensitivity of remote sensing in the Drought. The study showed that the NDWDI is more sensitive to regional drought than other absolute-soil-moisture-based indices. With the new index, the study extracted the spatial-temporal characteristics of the 2006 Drought, and explored its developing and withdrawing processes, which agreed with related statistics. Compared with ground method of drought observation, the NDWDI-based remote sensing solution of this paper is more pref- erable and practicable in that the local soil properties of water consumption and supply are implicitly taken into account, and the spatial representativity limit of ground observation is circumvented to a degree as satellite remotely senses the earth surface in a way of two-dimensional pixel matrix. So, the NDWDI-based method can be used to monitor regional soil water stress situation more practically and efficiently.

关键词： Sichuan Basin drought Normalized Difference Water Deviation Index response of remote sensing regional scale

The thymic stromal cell line MTSC4 induced thymocyte apoptosis in a non-MHC-restricted manner

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Cell Research 2004年第2期14卷 125-133页

作者： Xue Ying HE~1, Juan LI~1, Xiao Ping QIAN~1, Wen Xian FU~1, Yan LI~1, Li WU~2, Wei Feng CHEN~1 ~1Department of Immunology, peking university health science center,38 Xueyuan Road, beijing 100083, china. ~2The Walter and Eliza Hall institute, PO Royal Melbourne Hospital, Victoria 3050, Australia. Department of Immunology Peking University Health Science Center Beijing China The Walter and Eliza Hall institute PO Royal Melbourne Hospital Victoria Australia

Mouse thymic stromal cell line 4 （MTSC4） is one of the stromal cell lines established in our laboratory. While losing the characteristics of epithelial cells, they express some surface markers shared with thymic dendritic cells （TDCs）. To further study the biological functions of these cells, we compared the capability of MTSC4 with TDCs in the induction of thymocyte apoptosis, using thymic reaggregation culture system. Apoptosis of thymocytes induced by MTSC4 and TDCs was measured by Annexin V and PI staining and analyzed by flow cytometry. We found that MTSC4 selectively augmented the apoptosis of CD4+8+ （DP） thymocytes. This effect was Fas/FasL independent and could not be blocked by antibodies to MHC class Ⅰ and class Ⅱ molecules. In addition, MTSC4 enhanced the apoptosis of DP thymocytes from different strains of mice, which implies that MTSC4-induced thymocyte apoptosis is not mediated by the TCR recognition of self peptide/MHC molecules. In contrast to MTSC4, thymocyte apoptosis induced by TD

关键词： MHC thymic dendritic cells thymic stromal cell line thymocyte apoptosis.

Ectopic ATP synthase in endothelial cells:a novel cardiovascular therapeutic target

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岭南心血管病杂志 2011年第S1期17卷 35-36页

作者： Yi Zhu (Department of Physiology and Pathophysiology, peking university health science center,beijing 100191,china)

ATP synthase(ATPS) produces ATP in cells and is found on the inner membrane of mitochondria or the cell plasma *** this presentation, we will briefly summarize the functions of ecto-ATPS in vascular endothelial cells(ECs).Ecto -ATPS is involved in adenosine metabolism on the cell surface through its ATP generation or hydrolysis *** ATP/ADP generated by the enzyme on the plasma membrane can bind to P2X/P2Y receptors and activate the related signaling pathways to regulate endothelial ***-chain of ectopic ATP synthase(ATPS) on the EC surface can recruit inflammatory cells and activate cytotoxic activity to damage ECs and induce vascular *** and other angiogenesis inhibitors can have anti-angiogenic functions by inhibiting ecto-ATPS on ***-ATPS on ECs is also a receptor for apoA-Ⅰ, the acceptor of cholesterol efflux,which implies the involvement in cholesterol *** main talk will focus on our recent study about shear stress regulated membrane translocation of ATPS in ECs and the consequent interaction with T lymphocytes, which caused endothelial *** found that laminar flow decreased level of membrane-bound ATPS(ecto-ATPS) and depleted membrane cholesterol level in *** contract,oscillatory flow increased endothelial ecto-ATPS? and membrane cholesterol *** ECs with cholesterol or depleting cellular cholesterol could mimic the effect of oscillatory or laminar flow,*** of caveolin-1 expression by siRNA prevented ATPS translocation in response to shear ***, oscillatory flow elevated the number of T cells binding to ECs,and effect that could be blocked by anti-ATPS antibody;laminar flow significantly decreased this ***,the interaction of T cells and ATPS membrane translocation was elevated in the inner curvature of the aortic arch of apoE-/- mice fed a high-fat diet. Thus,our study provided the first evidence that disturbed flow and hyp

关键词： ATPS Ectopic ATP synthase in endothelial cells

Peroxisome proliferator-activated receptor-delta in metabolic syndrome:special emphasis on vessel and liver

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Peroxisome proliferator-activated receptor-delta in metaboli...

Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure

中国病理生理学会受体和信号转导专业委员会暨消化专业委员会联合学术会议

作者： Nanping Wang institute of cardiovascular science and Diabetes center,peking university,beijing 100191

Emerging evidence has revealed potential implications of peroxisome proliferator-activated receptor-δ(PPAR-δ) in lipid homeostasis,insulin resistance and cardiovascular ***,its pathophysiological roles and the underlying mechanisms remain to be *** functional genomic approach including gain- and loss-of-function studies,we have examined the effects of PPAR-8 on the expression of genes involved in the lipid biosynthesis in hepatocytes and the in vascular endothelial activation,major pathophysiological processes associated with metabolic *** results have demonstrated that,in hepatocytes,PPAR-δagonist as well as overexpression of PPAR-δinduced the gene expression of Insig-1,a regulatory endoplasmic reticulum protein braking SREBP activation. PPAR-δinhibited the proteolytic processing of the SREBP-1 into the mature active form,thereby suppressing the expression of the lipogenic genes such as fatty acid synthase,stearyl CoA desaturase-I and glycerol-3-phosphate *** effect is achieved by the induction of Insig-1,a direct target gene for PPAR-δFurthermore,overexpression of PPAR-δinduced Insig-1 expression and suppressed SREBP-1 activation,hence,limiting hepatic steatosis in obese diabetic *** vascular endothelial ceils (ECs),PPAR-δligands significantly inhibited TNF-α-induced expression of vascular cell adhesion molecule-1 and E-selectin as well as the ensuing endothelial-leukocyte ***-δligand upregulated the expression of antioxidant genes and decreased reactive oxygen species production in ECs. Ligand activation switched the association of BCL-6,a transcription repressor,from PPAR-δto the vascular cell adhesion molecule (VCAM)-1 *** interfering RNA reduced endogenous PPAR-δexpression but potentiated the suppressive effect of GW0742 on EC activation,suggesting that the nonliganded PPAR-δmay have an opposite *** together,our study reveals novel functions and the mechanism by which PPAR-δr

关键词： Nuclear receptor transcription factor lipogenesis gene expression diabetes

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science china(Life sciences) 2009年第11期52卷 997-1002页

作者： FENG YongJun1, ZHANG Meng2, HU MingXi1, ZHENG Jie1, JIAO WangWang2 & CHANG ZengYi2 1 School of Life science, beijing institute of Technology, beijing 100081, china 2 School of Life sciences, National Laboratory of Protein Engineering and Plant Genetic Engineering, center for Protein science, peking university, beijing 100871, china School of Life science Beijing Institute of Technology Beijing China School of Life sciences National Laboratory of Protein Engineering and Plant Genetic Engineering Center for Protein Science Peking University Beijing China

Many proteins exist as homo-oligomers in living organisms wherein the change of oligomeric status apparently serves as an effective means for modulating their biological activities. We have previously reported that the homo-decameric RbsD from Escherichia coli undergoes stepwise disassembly and non-stepwise reassembly. Here the structural status of the urea-induced RbsD disassembly intermediates was examined, mainly using urea-containing polyacrylamide gel electrophoresis and chemical cross-linking. Such intermediates were found to remain oligomeric while losing their intact secondary structures. Such disassembly intermediates were able to effectively refold when the concentration of the urea denaturant was reduced to a lower level, or to refold/reassemble into the native decamers when urea was completely removed, as detected by non-denaturing polyacrylamide gel electrophoresis. These novel observations strongly suggest that the assembly of oligomeric proteins may occur before the completion of subunit folding.

关键词： RbsD oligomeric protein oligomeric intermediate refolding reassembly

The size effect on transport properties of colossal magnetoresistance materials La_(0.67)Ca_(0.33)MnO_3

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science china(Physics,Mechanics & Astronomy) 2008年第3期51卷 251-257页

作者： LI PeiGang1,2,LEI Ming1,2,GUO YanFeng2,GUO Xi2,CHEN LeiMing2, TANG WeiHua1,2,SONG PengYun3 & CHEN JinPing3 1 Department of Physics,center for Optoelectronics Materials and Devices,Zhejiang Sci-Tech university,Hangzhou 310018,china 2 National Laboratory for Condensed Matter Physics,institute of Physics,Chinese Academy of sciences,beijing 100080,china 3 Department of Physics,peking university,beijing 100875,china Department of Physics Center for Optoelectronics Materials and Devices Zhejiang Sci-Tech University Hangzhou China National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing China Department of Physics Peking University Beijing China

High quality La0.67Ca0.33MnO3 (LCMO) film was deposited via a novel pulsed electron deposition technique on SrTiO3(100) single crystal substrate. The micro-bridge with different widths was fabricated by using electron beam lithography (EBL) technique and their transport properties were studied. For the micro-bridges with width of 2 and 1.5 μm,the insulator-to-metal transition temperature (TP) keeps un-changed compared with the film. For the micro-bridges with width of 1 μm,the TP shifts towards the lower temperature by 50 K. When the width decreases down to 500 nm,the insulator-to-metal transition disappears. The magnetoresistance be-havior of these micro-bridges was studied,and the results show that the low field magnetoresistance (LFMR) decreases and the high field magnetoresistances (HFMR) keep almost unchanged as the width of micro-bridge is reduced.

关键词： colossal magnetoresistance pulsed electron deposition electron beam lithography electrical transport properties