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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese academy of sciences, beijing 100080, china 2Center of Mass Spectrometry, Institute of Chemistry, chinese academy of sciences, beijing 100080, china 3graduate School of the chinese academy of sciences, beijing 100039, china Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China graduate School of the chinese academy of sciencesbeijing 100039 China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2 , XIANG Jianhai 1*, WANG Bing 1,2 , LI Fuhua 1 and TONG Wei 3(1. Experimental Marine Biology Laboratory, Institute of Oceanology, chinese academy of sciences, Qingdao 266071,china 2. graduate School of the chinese academy of sciences, beijing 100039, china 3. Genomics & Bioinformatics Institute, chinese academy of sciences, beijing 1013.0, china) chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult Fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： Fenneropenaeus chinensis bioinformatics ESTs immune factors

A Test on Different Types of the Time Curve of Hardness Ratio of Gamma-Ray Bursts based on the Curvature Effect

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chinese Journal of Astronomy and Astrophysics 2008年第4期8卷 451-464页

作者： Lan-Wei Jia National Astronomical Observatories/Yunnan Observatory,chinese academy of sciences,Kunming 650011,china graduate School of chinese academy of sciences,beijing 100049,china National Astronomical Observatories/ Yunnan Observatory Chinese Academy of Sciences Kunming 650011 China and graduate School of chinese academy of sciences Beijing 100049 China

We analyzed a sample of 66 gamma-ray bursts （GRBs） and statistically confirmed the prediction on the time curve of the hardness ratio of GRBs made by Qin et al. based on the curvature effect. In their analysis, GRB pulses are divided into three types according to the shape of their raw hardness ratio （RHR） time curves, defined as to include the background counts to the signal counts, so as to make use of counts within small time intervals. Of the three types, very hard sources exhibit a perfect pulse-like profile （type 1）, hard bursts possess a pulse-like profile with a dip in the decay phase （type 2）, and soft bursts show no pulse-like profile but have only a dipped profile （type 3）. In terms of the conventional hardness ratio, type 3 sources are indeed generally softer than those of type 1 and type 2, in agreement with the prediction. We found that the minimum value of RHR is sensitive in distinguishing the different types. We propose that GRB pulses can be classified according to the minimum value of RHR and that the different type sources may be connected with different strengths of the shock or/and the magnetic field.

关键词： gamma-rays burst -- gamma-rays observations - methods statistical

Quantitative analysis of soil pores under natural vegetation successions on the Loess Plateau

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Science china Earth sciences 2010年第4期53卷 617-625页

作者： ZHAO ShiWei1,ZHAO YongGang1,2 & WU JinShui3 1 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau,Institute of Soil and Water Conservation,Northwest Agriculture & Forestry university,Yangling 712100,china 2 graduate university of chinese academy of sciences,beijing 100049,china 3 Institute of Subtropical Agriculture,chinese academy of sciences,Changsha 410125,china 1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Institute of Soil and Water Conservation Northwest Agriculture & Forestry University Yangling 712100 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China3. Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 China

Soil pore is a key attribute of the soil structure that affects soil reservoir under natural vegetation recovery on the Loess *** study is to quantitatively analyze soil pore parameters,measured with Computed Tomography(CT) at 15-57 mm depths under five different vegetation succession stages using a concept of substituting "space" for "time" in the Ziwuling Forest Region of the Loess *** results showed that the soil pore parameters,such as pore number,porosity,circularity,and fractal dimension,increased significantly under the natural vegetation successions and varied with the pattern climax community stage>pioneering arbor community stage>scrub community stage>herbaceous community stage>abandoned farmland stage,indicating that natural vegetation recovery could remarkably improve soil pore *** the vegetation succession,this positive effect will be strengthened gradually,which means that the soil pore parameters may appear to be best at the climax community *** organic matter content increased linearly with the soil pore parameters(P<0.001).Increased organic matter accumulation was one of the major reasons for the changes in soil pore characteristics in natural vegetation succession.

关键词： Computed Tomography Loess Plateau natural vegetation succession soil pore soil organic matter

CONVERGENCE OF VORTEX METHODS FOR 3-D EULER EQUATIONS

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Journal of Computational Mathematics 2000年第3期18卷 239-250页

作者： Jia-fu Lin (Institute of System Science, academy of Mathematics and System sciences, chinese academy of sciences, beijing, 100080, china School of Mathematical sciences, Peking university, beijing 100871, china) 中科院系统科学所北京 100080

Presents a study on the convergence of vortex methods for the three-dimensional Euler equations in bounded domains. Related studies on the convergence of vortex methods; Formulation of vortex methods; Convergence for ... 详细信息

关键词： Euler equations vortex methods convergence initial-boundary value problem

Sea level variations in the South china Sea inferred from satellite gravity,altimetry,and oceanographic data

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Science china Earth sciences 2012年第10期55卷 1696-1701页

作者： FENG Wei 1,2,ZHONG Min 1,3 & XU HouZe 1,3 1 State Key Laboratory of Geodesy and Earth’s Dynamics,Institute of Geodesy and Geophysics,chinese academy of sciences,Wuhan 430077,china 2 graduate university of chinese academy of sciences,beijing 100049,china 3 Institute of Geophysics,Huazhong university of Science and Technology,Wuhan 430074,china 1. State Key Laboratory of Geodesy and Earth’s Dynamics Institute of Geodesy and Geophysics Chinese Academy of Sciences Wuhan 430077 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China3. Institute of Geophysics Huazhong University of Science and Technology Wuhan 430074 China

Sea level variations(SLVs) can be divided into two major components:the steric SLV and the mass-induced *** two components of SLV in the South china Sea(SCS) are studied by using satellite altimetry,GRACE(Gravity Recovery and Climate Experiment) satellite gravity,and oceanographic data on annual and inter-annual *** the annual timescale,the geographic distribution of mass-induced SLV's amplitude jointly estimated from altimetry and the ECCO(Estimation of the Circulation and Climate of the Ocean) model agrees very well with that from *** observes obvious seasonal mass-induced SLV in the SCS with annual amplitude of 2.7±0.4 cm,which is consistent with the annual amplitude of 2.7±0.3 cm estimated from the steric-corrected *** the inter-annual timescales,the mean SLV in the SCS shows a large oscillation,which is mainly caused by the steric *** trend of mean SLV inferred from altimetry in the SCS is 5.5±0.7 mm/yr for the period of 1993-2009,which is significantly higher than the global sea level rise rate of 3.3±0.4 mm/yr in the same *** is no obvious trend signal in the mass-induced SLV detected from GRACE that indicates the water exchange between the SCS and its adjacent seas and land is in balance within the study period.

关键词： altimetry GRACE ECCO the South china Sea sea level variation

Regional soil moisture simulation for Shaanxi Province using SWAT model validation and trend analysis

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Science china Earth sciences 2010年第4期53卷 575-590页

作者： LI MingXing1,2,MA ZhuGuo1 & DU JiWen3 1 Key Laboratory of Regional Climate-Environment Research for Temperate East Asia,Institute of Atmospheric Physics,chinese academy of sciences,beijing 100029,china 2 graduate university of chinese academy of sciences,beijing 100049,china 3 Shaanxi Administration of Meteorology,Xi’an 710014,china 1. Key Laboratory of Regional Climate-Environment Research for Temperate East Asia Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China3. Shaanxi Administration of Meteorology Xi’an 710014 China

The soil moisture in Shaanxi Province,a region with complex topography,is simulated using the distributed hydrological model Soil Water Assessment Tool(SWAT).Comparison and contrast of modeled and observed soil moisture show that the SWAT model can reasonably simulate the long-term trend in soil moisture and the spatiotemporal variability of soil moisture in the *** to NCEP/NCAR and ERA40 reanalysis of soil moisture show that the trend of variability in soil moisture simulated by SWAT is more consistent with the *** model results suggested that high soil moisture in surface soil layers appears in the southern Shaanxi with high vegetation cover,and the Qinling mountainous region with frequent orographic *** deeper soil layers,high soil moisture appears in the river basins and *** regional soil moisture showed a generally decreasing trend on all soil layers from 1951 to 2004,with a stronger and significant decreasing trend in deeper soil layers,especially in the northern parts of the province.

关键词： SWAT soil moisture content simulation validation trend analysis

Improving the accuracy of image-based forest fire recognition and spatial positioning

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Science china(Technological sciences) 2010年第S1期53卷 184-190页

作者： JIANG LiLi 1,QI QingWen 1,ZHANG An 1,GUO ChaoHui 2 & CHENG Xi 1,3 1 Institute of Geographical sciences and Natural Resources Research,chinese academy of sciences,beijing 100101,china 2 china Center for Resources Satellite Data and Applications,beijing 100094,china 3graduate university of chinese academy of sciences,beijing 100049,china chinese Acad Sci Inst Geog Sci & Nat Resources Res Beijing 100101 Peoples R China china Ctr Resources Satellite Data & Applicat Beijing 100094 Peoples R China chinese Acad Sci Grad Univ Beijing 100049 Peoples R China

Forest fires are frequent natural *** is necessary to explore advanced means to monitor,recognize and locate forest fires so as to establish a scientific system for the early detection,real-time positioning and quick fighting of forest *** paper mainly expounds methods and algorithms for improving accuracy and removing uncertainty in image-based forest fire recognition and spatial ***,we discuss a method of forest fire recognition in visible-light *** are four aspects to improve accuracy and remove uncertainty in fire recognition:(1)eliminating factors of interference such as road and sky with high brightness,red leaves,other colored objects and objects that are lit up at night,(2)excluding imaging for specific periods and azimuth angles for which interference phenomena repeatedly occur,(3)improving the thresholding method for determining the flame border in image processing by adjusting the threshold to the season,weather and region,and (4)integrating the visible-light image method with infrared image ***,we examine infrared-image-based methods and approaches of improving the accuracy of forest fire recognition by combining the spectrum threshold with an object feature value such as the normalized difference vegetation index and excluding factors of disturbance such as interference signals,extreme weather and high-temperature ***,a method of visible analysis to enhance the accuracy of forest fire positioning is examined and realized;the method includes decreasing the visual angle,selecting central points,selecting the largest spots,and judging the selection of fire spots according to the central *** studies are examined and the results are found to be satisfactory.

关键词： forest fire monitoring spatial positioning accuracy improvement

A mixed method for measuring low-frequency acoustic properties of macromolecular materials

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 729-739页

作者： LIU Hongwei1,YAO Lei1,3,ZHAO Hong1,3,ZHANG Jichuan2,3 & XUE Zhaohong2 1. National Key Laboratory of Acoustics,Institute of Acoustics,chinese academy of sciences,beijing 100080,china 2. Institute of Chemistry,chinese academy of sciences,beijing 100080,china 3. graduate university of chinese academy of sciences,beijing 100049,china National Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences Beijing 100080 China Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China graduate university of chinese academy of sciences Beijing 100049 China

A mixed method for measuring low-frequency acoustic properties of macro-molecular materials is presented. The dynamic mechanical parameters of materials are first measured by using Dynamic Mechanical Thermal Apparatus(DMTA) at low frequen-cies,usually less than 100 Hz; then based on the Principles of Time-Temperature Super-position (TTS),these parameters are extended to the frequency range that acousticians are concerned about,usually from hundreds to thousands of hertz; finally the extended dynamic mechanical parameters are transformed into acoustic parameters with the help of acoustic measurement and inverse analysis. To test the feasibility and accuracy,we measure a kind of rubber sample in DMTA and acquire the basic acoustic parameters of the sample by using this method. While applying the basic parameters to calculating characteristics of the sample in acoustic pipe,a reasonable agreement of sound absorp-tion coefficients is obtained between the calculations and measurements in the acoustic pipe.

关键词： low-frequency acoustic properties,dynamic mechanical properties,Principles of Time-Temperature Superposition,acoustic pipe,sound absorption coefficient.