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精氨酸对慢性阻塞性肺疾病合并营养不良的免疫调理作用

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中国实用内科杂志 2003年第9期23卷 527-529页

作者：徐卫国罗勇杨玲上海第二医科大学附属新华医院呼吸内科 200092

目的　研究精氨酸对慢性阻塞性肺疾病 (COPD)合并营养不良患者的免疫调理作用。方法　对 30例营养不良的COPD病人进行总能量摄入均为静息能量消耗 (REE) 1 5倍的营养支持。A组 (12例 )为精氨酸治疗组 ,每日口服L 精氨酸 30 g ,B组 (18... 详细信息

目的　研究精氨酸对慢性阻塞性肺疾病 (COPD)合并营养不良患者的免疫调理作用。方法　对 30例营养不良的COPD病人进行总能量摄入均为静息能量消耗 (REE) 1 5倍的营养支持。A组 (12例 )为精氨酸治疗组 ,每日口服L 精氨酸 30 g ,B组 (18例 )为对照组。结果　A组TSF、PAlb、CHI等营养参数明显较B组改善 ,A组TNF α、CD8治疗后显著下降 ,而IL 2、NO、IgG、CD4 等显著上升 (P <0 0 5 )。结论　精氨酸对COPD合并营养不良患者不仅具有辅助营养支持作用 ,而且还有免疫调理作用。

关键词：精氨酸慢性阻塞性肺疾病营养不良免疫调理作用合并症

Increased frequency of the CTLA-4 +49A>G polymorphism in Chinese patients with colorectal cancer compared to healthy controls

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Increased frequency of the CTLA-4 +49A>G polymorphism in Chi...

全国临床免疫检验研讨会暨第六届全国临床免疫学术会议

作者： Peng Qi~1,Can-ping Ruan~2,Tong-hai Dou~3,Hao Wang~4,Fei-guo Zhou~5,Xin-yun Xu~2,Xing Gu~1,Yun-peng Zhao~1,Chun-fang Gao~(*1) 5.department of Laboratory medicine,Eastern Hepatobiliary hospital,second Military medical university,shanghai 200438,china 6.department of General Surgery,Changzheng hospital,second Military medical university,shanghai 200003,china 7.Institute of Genetics,Fudan university,shanghai 20043,china 8.department of Laboratory medicine,Changzheng hospital,second Military medical university,shanghai 200003,china 9.department of Hepatic Surgery,Eastern Hepatobiliary hospital,second Military medical university,shanghai 200438,china.

Objective Colorectal cancer is one of the most common malignancies in the world and a multipathway *** T-lymphocyte antigen-4(CTLA-4) is a potent immunoregulatory molecule that suppresses antitumor response by down-regulating T-cell *** most studied +49A>G polymorphism of CTLA-4 gene has been associated with various kinds of *** aim was to investigate the association between this genetic variant and the risk and progression of colorectal cancer in Chinese. Method We conducted a case-control study of 124 colorectal cancer cases and 407 healthy *** was extracted from blood specimens,and +49A>G polymorphism in the CTLA-4 gene was genotyped by polymerase chain reaction-ligation detection reaction(PCR-LDR).Results In our study group,the frequency of AG or GG or carrying at least one G allele at position +49 was significantly different in colorectal cancer patients and the control group,indicating that the risk of CRC was significantly higher among subjects with the AG or GG genotype or carrying at least one G allele at position +49 than among the subjects with the AA ***,we observed no association between CTLA-4 +49A>G polymorphism and the progression of CRC including tumor stage,tumor grade,status of lymph node and distant ***,the CTLA-4 +49A allele was in non-significantly higher numbers in CRC patients with distant *** Our results suggested that CTLA-4 +49A>G polymorphism was associated with an increased risk of colorectal cancer,but this polymorphism did not play an important role in Chinese patients with CRC.

关键词： Colorectal cancer cytotoxic T-lymphocyte antigen-4 single-nucleotide polymorphism case-control study individual susceptibility

N-glycan Based Models Improve Diagnostic Efficacies in HBV-related Hepatocellular Carcinoma

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N-glycan Based Models Improve Diagnostic Efficacies in HBV-r...

全国临床免疫检验研讨会暨第六届全国临床免疫学术会议

作者： Meng Fang,MD,Yun-Peng Zhao,MD,Lun-Gen Lu,MD,Peng Qi,Fei-Guo Zhou,MD,Hao Wang,Kun Zhou,Shu-Han Sun,PhD,Cui-Ying Chen,PhD,Chun-Fang Gao,MD 1 department of Laboratory medicine,Eastern Hepatobiliary hospital,second Military medical university, shanghai,china 2 department of Gastroenterology,renji hospital,Jiaotong university School of medicine,shanghai,china 3 department of Laboratory medicine,Changzheng hospital,second Military medical university,shanghai, china 4 department of Genetics,second Military medical university,shanghai,china 5 department for Molecular Biomedical Research,Ghent university and Flanders Interuniversity Institute for Biotechnology(VIB),Ghent,Belgium.

BACKGROUND:The early diagnosis of hepatocellular carcinoma(HCC) is of great clinical desirable due to lack of specific and sensitive *** in the sugar chains of glycoprotein synthesized by the liver contribute to the molecular basis of abnormalities in *** the study aims to construct and assess the diagnostic value of N-glycan based diagnostic model in identifying ***:A total of 273 cases HBV related HCC(n=145),liver fibrosis(n=128) and matched healthy controls (n=120) were recruited.N-glycome of serum protein was profiled by DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis(DSA-FACE). Multiparameters diagnostic models were constructed based on above profiling and routine ***:A biantennary glycan(NG1A2F,peak 4) was found to be decreased in HCC and increased in liver fibrosis,while a branching triantennary glycan(NA3Fb,peak 9) was increased in HCC and decreased in liver *** efficacy of the log ratio of these two N-glycan structure abundances[log(p9/4)]was similar to AFP in differentiation HCC from *** accuracy and sensitivity of the diagnostic model combining AFP and N-glycan markers(Cscore B)were increased 7-10%compared to that of AFP. Log(p9/4) was more efficient in monitoring the progression of HCC in regarding to vascular invasion with improved specificity(16%) and accuracy(8%) compared to that of AFP. CONCLUSIONS:The branchingα(1,3)-fucosylated triantennary glycan and the biantennary glycan are potential as N-glycan *** diagnostic models based on the N-glycan markers and AFP improve the efficacy in HCC diagnosis and progression monitoring.

关键词： hepatocellular carcinoma(HCC) liver fibrosis hepatitis B virus(HBV) N-glycan marker

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重叠综合征患者夜间缺氧和睡眠结构的分析

上海医学 2003年第12期26卷 899-901页

作者：王佳林建海鲁慧敏上海第二医科大学附属新华医院呼吸科 200092

目的　探讨重叠综合征 (OS)患者夜间缺氧和睡眠结构的变化。方法　对 19例OS、2 5例阻塞性睡眠呼吸暂停综合征 (OSAS)、17例慢性阻塞性肺疾病 (COPD)和 14例鼾症患者分别进行全夜多导睡眠仪 (PSG)检查 ,并对各组数据作统计学t检验。结... 详细信息

目的　探讨重叠综合征 (OS)患者夜间缺氧和睡眠结构的变化。方法　对 19例OS、2 5例阻塞性睡眠呼吸暂停综合征 (OSAS)、17例慢性阻塞性肺疾病 (COPD)和 14例鼾症患者分别进行全夜多导睡眠仪 (PSG)检查 ,并对各组数据作统计学t检验。结果　单纯鼾症患者基本不存在日间或夜间缺氧 (P 3+ 4 )显著低于COPD患者 (P <0 .0 1) ,动脉血氧饱和度 (SaO2 ) <90 %累计时间的百分率 (SLT90 % )、SaO2 <80 %累计时间占睡眠时间的百分率 (SLT80 % )及浅睡眠所占百分率 (S1+ 2 )显著高于COPD患者 (P <0 .0 1)。OS患者SLT90 %、S1+ 2显著高于OSAS患者 (P <0 .0 1) ,睡眠效率显著低于OSAS患者 (P <0 .0 1)。结论　OS患者夜间缺氧较严重 ,睡眠结构改变明显。

关键词：重叠综合征夜间缺氧睡眠结构阻塞性睡眠呼吸暂停综合征慢性阻塞性肺疾病鼾症多导睡眠仪夜间低氧血症

活血方对免疫介导再生障碍性贫血小鼠IL-3、IFN-γ的影响

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复旦学报（医学版） 2003年第6期30卷 553-555页

作者：丁敬远黄振翘孙关林盛民立周永明黄韬王克强薛志忠上海华东疗养院中医科无锡214065 上海中医药大学附属岳阳医院血液科上海200437 上海第二医科大学附属瑞金医院血液科上海200025 复旦大学放射医学研究所第四研究室上海200032 复旦大学附属中山医院心血管病研究所上海200032

目的检测免疫介导再生障碍性贫血(再障)小鼠骨髓造血细胞在含活血药血清液体培养体系中IL-3、IFN-γ的浓度,探讨活血方药治疗再障的作用机制。方法用活血方中药煎剂灌喂SD大鼠,抽取、制备含中药血清;Balb/c小鼠经60Co 5.5 Gy γ射线... 详细信息

目的检测免疫介导再生障碍性贫血(再障)小鼠骨髓造血细胞在含活血药血清液体培养体系中IL-3、IFN-γ的浓度,探讨活血方药治疗再障的作用机制。方法用活血方中药煎剂灌喂SD大鼠,抽取、制备含中药血清;Balb/c小鼠经60Co 5.5 Gy γ射线照射后,由尾静脉输入取自DBA/2小鼠胸腺淋巴结混合细胞悬液,形成免疫介导再障模型。在含活血药血清的液体培养体系中培养再障小鼠骨髓造血细胞,检测培养体系中IL-3、IFN-γ的浓度。结果正常活血血清组IL-3浓度显著高于正常对照血清组(P3浓度则显著低于再障对照血清组(P0.05)。结论活血方药临床治疗再障的作用可能是影响IL-3的生成而进一步促进造血干/祖细胞增殖、分化。

关键词：活血方免疫介导再生障碍性贫血小鼠 IL-3 IFN-γ 白细胞介素3 γ-干扰素

Therapeutic Angiogenesis:The Combination Point of Chinese and Western medicine in Prevention and Treatment of Ischemic Heart Disease

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Chinese Journal of Integrative medicine 2009年第5期15卷 323-324页

作者：吴宗贵 department of Cardiology Changzheng HospitalSecond Military Medical University

At present, the conventional medicine and revascularization （including interventional treatment and coronary bypass surgery） in ischemic heart disease have limited the clinical efficacy on the progress of the left ventricular remodeling and heart failure after myocardial infarction, and the mortality accounts about 50% of cardiovascular diseases. With the potential role of repairing injured or necrotic myocardium and improving the blood supply in ischemic region, heart cell therapy and therapeutic angiogenesis have become new highlights in disease treatment and the field of cardiovascular new strategies for the prevention and treatment of complications of endstage ischemic heart diseases.

关键词： Therapeutic Angiogenesis

Long non-coding RNA high expressed in hepatocellular carcinoma(IncRNA-HEIH) facilitates tumor growth through enhancer of zeste homolog 2

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Long non-coding RNA high expressed in hepatocellular carcino...

遗传学进步促进粮食安全与人口健康高峰论坛

作者： Fu Yang1,Ling Zhang1,Xi-song Huo1,Ji-hang Yuan1,Dan Xu1,Sheng-xian Yuan2,Nan Zhu3,Wei-ping Zhou2,Guang-shun Yang3,Yu-zhao Wang1,Jing-li Shang1,Chun-fang Gao4,Feng-rui Zhang5,Fang Wang1,Shu-han Sun1 department of medical Genetics,second Military medical university,shanghai,china 2 The Third department of Hepatic Surgery,Eastern Hepatobiliary hospital,second Military medical university,shanghai,china 3 The second department of Hepatic Surgery,Eastern Hepatobiliary hospital,second Military medical university, shanghai,china 4 department of Laboratory medicine,Eastern Hepatobiliary hospital,second Military medical university,shanghai,china 5 department of Hepatobiliary Surgery,Hebei Cancer hospital,Hebei medical university,Hebei,china.

Hepatocellular carcinoma(HCC) is one of the most common human cancers worldwide, particularly in Southeast Asia and *** than 70-80%of HCC cases occur in high hepatitis B virus(HBV) endemic regions,and 50%of HCC cases worldwide are attributable to chronic infection with ***,the 5-year survival rate of HBV-related HCC patients remains poor,and approximately 600,000 HCC patients die each year despite recent advances in surgical techniques and medical *** previous studies identified many aberrantly expressed protein-coding genes in HCC,novel molecular markers that can help in early diagnosis and risk assessment are still urgently *** is of paramount importance to understand the relationships between clinical symptoms and molecular changes in HCC for developing new diagnosis and treatment strategies for HCC and improving the prognosis of diagnosed patients. The human transcriptome comprises not only large numbers of protein-coding messenger RNAs(mRNAs) but also a large set of non-protein coding transcripts that have structural,regulatory or unknown *** studies of small non-coding RNAs(18 to 200 nucleotides(nt)) have dominated the field of RNA biology in recent years,a surprisingly wide array of cellular functions has also been associated with long non-coding RNAs(IncRNAs).IncRNAs,tentatively defined as non-coding RNAs more than 200 nt in length,are characterized by the complexity and diversity of their sequences and mechanisms of action.A handful of studies have implicated IncRNAs in a variety of disease states,and altered IncRNA levels can result in aberrant expression of gene products that may contribute to cancer ***,there are only preliminary studies on the role of IncRNAs in HCC,and the overall pathophysiological contributions of IncRNAs to HCC remain largely unknown. In this study,we have identified non-overlapping signatures of a small number of IncRNAs that are aberrantly expressed in human HBV

关键词： hepatocellular carcinoma noncoding RNAs PRC2 IncRNAs cell cycle

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重症肺炎患者中性粒细胞胞吐作用的改变

上海医学 2004年第4期27卷 250-252页

作者：陈吉泉颜泽敏修清玉第二军医大学长征医院呼吸内科上海200003

目的　研究重症肺炎患者中性粒细胞的功能改变。方法　采用免疫化学法测定 32例重症肺炎患者中性粒细胞胞吐作用和氧化酶反应的变化 ,并与健康对照组比较。结果　重症肺炎患者血液中性粒细胞的基础和PMA刺激的胞吐作用均明显下降 (P <0 ... 详细信息

目的　研究重症肺炎患者中性粒细胞的功能改变。方法　采用免疫化学法测定 32例重症肺炎患者中性粒细胞胞吐作用和氧化酶反应的变化 ,并与健康对照组比较。结果　重症肺炎患者血液中性粒细胞的基础和PMA刺激的胞吐作用均明显下降 (P <0 .0 1) ;重症肺炎患者的基础和PMA刺激的反应性氧属 (ROS)产生明显增加 ;重症肺炎患者肺部中性粒细胞释放乳铁蛋白、髓过氧化物酶 (MPO)和ROS增加 ,PMA刺激后相似 ;肺炎的严重性与血液中性粒细胞释放乳铁蛋白呈负相关。结论　重症肺炎患者中性粒细胞胞吐作用明显受损 ,与预后相关。

关键词：肺炎中性粒细胞胞吐作用免疫化学法

Screening of GJB2 mutations in Chinese population

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Journal of Otology 2007年第1期2卷 18-22页

作者： HAN Ming-kun1, HAN Dong-yi1, GUO Yu-fen2, LI Qing-zhong3, ZHAO Ya-li1, 4, RAO Shao-qi1, 5, 6, YUAN Hu1, ZONG Liang1, GUAN Jing2, XU Bai-cheng2, WANG Da-yong1, LAN lan1, WANG Qiu-ju1, 7 1 department of Otorhinolaryngology/ Head and Neck Surgery, and Institute of Otolaryngology, china People Liberation Army General hospital, Beijing, china 2 department of Otorhinolaryngology/ Head and Neck Surgery, the second Affiliated hospital of LanZhou university, Lanzhou, china 3 Deparment of Otlolaryngology, Eye and ENT hospital of Fudan university, shanghai, china 4 department of Biochemistry and Molecular Biology, Institute of Basic medical Sciences, Chinese Academy of medical Sciences and Peking Union medical College, Beijing, china 5 departments of Cardiovascular medicine and Molecular Cardiology, the Cleveland Clinic Foundation, Cleveland, Ohio, USA 6 department of Bioinformatics, Harbin medical university, Harbin, china 7 china National Human Genome Center, Beijing, china department of Otorhinolaryngology/ Head and Neck Surgery and Institute of Otolaryngology China People Liberation Army General Hospital Beijing China department of Otorhinolaryngology/ Head and Neck Surgery the Second Affiliated Hospital of LanZhou University Lanzhou China Deparment of Otlolaryngology Eye and ENT Hospital of Fudan University Shanghai China department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China departments of Cardiovascular medicine and Molecular Cardiology the Cleveland Clinic Foundation Cleveland Ohio USA department of Bioinformatics Harbin Medical University Harbin China china National Human Genome Center Beijing China

The GJB2 gene(connexin 26) has been shown to be responsible for DFNB1 and DFNA3. We screened the GJB2 gene in 488 patients with prelingual deafness(Group 1), 124 with postlingual deafness(Group 2), and 117 normal hearing subjects(Group 3). We found that, in Group 1, 65 patients(13.32%) were homozygotes or compound heterozygotes and 51 patients (10.45%) carried a single pathogenic mutation. The 235delC mutation was the most frequent mutation, accounting for 73.22% of the known pathogenic alleles in Group 1. No homozygotes or compound heterozygotes were detected in Group 2 or Group 3. Some postlingual deaf patients (2.42%) and normal hearing subjects(4.27%) were 235delC carriers. Our preliminary data indicate that 235delC, the most frequent mutation identified in this study, is a major cause for prelingual deafness.

关键词： prelingual deafness postlingual deafness, GJB2 235delC Chinese