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Polyclonal antibody production and expression of CREG protein in human vascular smooth muscle cells

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Journal of Geriatric Cardiology 2005年第2期2卷 118-122页

作者： Yaling HAN Haiwei LIU Jian KANG Xiaozeng WANG Ye HU Lianyou ZHAO Shaohua LI department of Cardiology General Hospital of PLA Shenyang Command Shenyang 110016 China Department of Cardiology General Hospital of PLA Shenyang Command Shenyang 110016 China Department of Cardiology General Hospital of PLA Shenyang Command Shenyang 110016 China Department of Cardiology General Hospital of PLA Shenyang Command Shenyang 110016 China Department of Cardiology General Hospital of PLA Shenyang Command Shenyang 110016 China Department of Cardiology Tangdu Hospital Fourth Military Medical University Xi''an 710038 China Department of Pathology and Laboratory Medicine Robert Wood Johnson Medical School New Jersey 08854 USA￡(C)

Objectives The cellular repressor of E1A-activated genes (CREG), a novel gene, was recently found to play a role in inhibiting cell growth and promoting cell differentiation. The purpose of this study was to obtain antibody against CREG protein and to study the expression of CREG protein in human internal thoracic artery cells (HITASY) which express different patterns of differentiation markers after serum withdrawal. Methods The open reading frame of CREG gene sequence was amplified by PCR and cloned into the pGEX-4T-1 vector. Glutathione-S-transferase (GST)-CREG fusion protein was expressed in E. Coli BL21 and purified from inclusion bodies by Sephacryl S-200 chromatography. Rabbits were immunized with the purified GST-CREG protein. Western blot examined with immunohistochemistry staining and the protein expression level was analyzed by Western blot in HITASY cells after serum removal. Results It was confirmed by using endonuclease digesting and DNA sequencing that the PCR product of CREG was correctly inserted into the vector. The GST-CREG protein was purified with gel filtration chromatography. Polyclonal antibody against GST-CREG was obtained from rabbits. CREG protein immunohistochemistry staining displayed a perinuclear distribution in the cytoplasm of HITASY cells. Results from Western blot suggested that comparing with the untreated cells upregulation of CREG polyclonal antibody against CREG was comfirmed. Using this antibody, the changes of CREG protein expression was observed in the process of phenotypic modulation of HITASY cells. These results provide basic understanding on the relationship of CREG gene with the cell phenotypic conversion.

关键词： E1A cellular repressor polyclonal antibody vascular smooth muscle cells differentiation

Research on Management of Modern Science and Technology Assessment Process and System Construct（ID:P-007）

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Research on Management of Modern Science and Technology Asse...

第十四届工业工程与工程管理国际会议

作者： Shen Ying~(1 2) Zhao Shukuan~1 Chen Xiuli~3 1 school of Management Jilin university Changchun P.R.china 130025 2 ChangChun university of Technology ChangChun P.R.china 130012 3 Management department of Hebei Institute of Architecture Civil Engineering Zhangjiakou P.R.china 075024

＜正＞Science and technology assessment is a part of management of science and technology,thought reeent years for its overlap with other act of MOT such as technology forecasting assessment is ignored to a certain *** science and technology assessment theory is important to science and technology for all levels（nation and company）. This paper study on the modem science and technology assessment theory,especially on management of assessment process and system construct,for they are play an useful role to MOT and science and technology *** science and technology assessment is merely provide information in decision making,the significance of the assessment process is obvious too,so management of assessment process is an important part of modem science and technology as- sessment *** is a dynamic process,include the occasion of assessment（the time to put forward assess）, the support and management of information and intelligence,control of assessment program,application of assessment result and the result *** on the construct of assess system is another important part of modem science and technology assessment *** system includes many sub-systems and is supported by many outside sys- tems,hut the emphasis is build and management of the assessment information *** system is not only important to assessment theory and action,but also important to MOT,especially *** paper researches on the management of modem science and technology assessment process and detailedly analyzes the construct of assess- ment system for different levels.

关键词： Science And Technology Assessment MOT Assessment Process System Construct

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美式橄榄球对中国武术文化发展的启示

体育学刊 2008年第8期15卷 22-25页

作者：蒋明朗栾小燕薛宇咸阳师范学院体育系陕西咸阳712000 西安体育学院研究生部陕西西安710068 北京体育大学研究生院北京100084

通过对美国橄榄球运动发展的探讨,得出其成功经验有:坚实的群众基础、良好的经济运作模式、明确的文化定位、合理的比赛规则。认为中国武术的发展应借鉴其经验,积极推进武术与学校体育的结合,培养雄厚的人才基础;通过建立完善的武术市... 详细信息

通过对美国橄榄球运动发展的探讨,得出其成功经验有:坚实的群众基础、良好的经济运作模式、明确的文化定位、合理的比赛规则。认为中国武术的发展应借鉴其经验,积极推进武术与学校体育的结合,培养雄厚的人才基础;通过建立完善的武术市场发展体系,形成完善的市场开发体系和产业结构,从而使武术建立自我发展和完善的运行机制;坚持民族特色,以自我发展为基础,不应为适应国际化而丢失自己的文化特色,在此基础上,进一步强化武术文化与中国新时期文化建设的契合。坚持以人为本,坚持对项目进行合理化的改造,改造的内容包括规则、防护用品、场地等,使武术成为既有丰富的传统文化内涵,又安全健康的人们乐于参加的体育运动。

关键词：体育文化武术橄榄球

Separate critical condition for ultrasonic vibration assisted grinding

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Journal of Shanghai university(English Edition) 2009年第5期13卷 391-395页

作者：张洪丽张建华 department of Construction Machinery Shandong Jiaotong University school of Mechanical Engineering Shandong University

Separate characteristic of the tangential ultrasonic vibration assisted grinding (TUAG) machining is analyzed based on TUAG process, and a critical speed formula is given to correctly set the machining parameters to insure the separate characteristics of TUAG process. The critical speed is not only related to the ultrasonic vibration amplitude and frequency, but also to the grinding wheel velocity and the cutting point space, and the grinding force can be decreased during the TUAG process with separability. Grinding force experiments are conducted, and the experimental results are in good agreement with the theoretical results.

关键词： tangential ultrasonic vibration assisted grinding (TUAG) critical speed separate characteristic

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区间数多指标决策的相对隶属度法

系统工程与电子技术 2004年第7期26卷 903-905,913页

作者：刘华文姚炳学山东大学数学与系统科学学院山东济南250100

对区间数多指标决策问题 ,基于模糊集下论给出一种新的处理方法 :相对隶属度法 ,首先 ,构造优等与劣等方案 ,借助每一方案与优、劣等方案的综合加权距离求得每个方案相于优等方案的隶属度 ,再按隶属度的大小进行决策 ,该方法克服了以往... 详细信息

对区间数多指标决策问题 ,基于模糊集下论给出一种新的处理方法 :相对隶属度法 ,首先 ,构造优等与劣等方案 ,借助每一方案与优、劣等方案的综合加权距离求得每个方案相于优等方案的隶属度 ,再按隶属度的大小进行决策 ,该方法克服了以往研究此类问题时所遇到的区间数难以排序的困难。通过分析表明相对隶属度法的择优与排序能力要比传统的逼近理想解法强。最后。

关键词：多指标决策相对隶属度法模糊集区间数

Small-molecule fluorescent probes for theα1-adrenergic receptors

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Small-molecule fluorescent probes for theα1-adrenergic rece...

2011年全国药物化学学术会议——药物的源头创新

作者： Wenhua Li,Lupei Du and Minyong Li~* department of Medicinal Chemistry,school of Pharmacy,Shandong university,Shandong 250012, china

＜正＞ai-adrenergic receptors（ARs）,as the crucial members of G protein-coupled receptors（GPCRs）, draw more attention of the medicinal chemists because they mediate a number of physiological responses of the sympat... 详细信息

关键词： ai-adrenergic receptors small-molecule fluorescent probes

The effects of thyroid hormone on the expression of connexin 43,45 and 32 In the astrocyte(Thyrold hormone and connexin)

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The effects of thyroid hormone on the expression of connexin...

中华医学会第十次全国内分泌学学术会议

作者： Chaochun Zou,Zhengyan Zhao Children’s Hospital of zhejiang university school of Medicine

Objective:s To investigate the effect of thyroid hormone (TH) on connexin(Cx) expression by measuring the Cx43,Cx45 and Cx32 levels in astrocytes.

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Synthesis and Biological Evaluation of Novel Non-Peptideαvβ3 Integrin Receptor Antagonists

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Synthesis and Biological Evaluation of Novel Non-Peptideαv...

2011年全国药物化学学术会议——药物的源头创新

作者： Zhixiong Huang XiaodongJing Yong Deng~* department of Medicinal Chemistry,West china school of Pharmacy,Sichuan university,Chengdu 610041

Malignant tumor is one of the main diseases that threaten human’s health and *** metastasis and multidrug resistance of tumor cells to chemotherapy agents are the mainly difficult problems in clinical *** studies indicate that integrinαβ,which plays a key role in tumor-induced angiogenesis and metastasis,is the effective target to design antiangiogenic and

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Decreased cardiac sarcoplasmic reticulum Ca2+-ATPase activity contributes to cardiac dysfunction in streptozotocin-induced diabetic rats

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Decreased cardiac sarcoplasmic reticulum Ca2+-ATPase activit...

2006年浙江省心血管病学学术年会

作者： Xiao-yan ZHAO, PhD Shen-jiang HU, PhD, MD Jiang LI, PhD Yun MOU, PhD Bao-ping CHEN, PhD QiangXIA, PhD institute of Cardiology The First Affiliated Hospital College of Medicine, zhejiang university hangzhou, china department of physiology College of Medicine, zhejiang university hangzhou, china

Diabetic cardiomyopathy is characterized by reduced cardiac contractility due to direct changes in myocardium function independent of vascular disease. This study is to investigate the alterations of cardiac sarcoplasmic reticulum Ca2+-ATPase activity and cardiac function in streptozotocin-induced diabetic rats. Diabetes (DM) was induced in male Wistar rats by intraperitoneal injection of streptozotocin. The activity of sarcoplasmic reticulum Ca2+-ATPase and the left ventricular hemodynamic parameters were measured in

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