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A novel mutation at the JK locus causing Jknull phenotype in a Chinese family

Science China(Life Sciences) 2005年第6期48卷 636-640页

作者： MENG Yan1, ZHOU Xueyan2, LI Yang1, ZHAO Dan1, LIANG Shuyuan2, ZHAO Xuejian1, YANG Baoxue1,3 1. department of Pathophysiology, School of Medical Science, Jilin university, Changchun 130021, China 2. The Third Hospital of Jilin university, Changchun 130031, China 3. departments of medicine, university of california, san francisco, ca, 94143-0521, usa department of Pathophysiology School of Medical Science Jilin University Changchun 130021 China The Third Hospital of Jilin university Changchun 130031 China department of Pathophysiology School of Medical Science Jilin University Changchun 130021 China departments of medicine University of California San Francisco CA 94143-0521 USA

Urea transporters are a group of proteins that facilitate urea movement across biological membranes. Kidd blood group (JK antigen) and urea transporter of human erythrocytes are carried by the same protein UT-B. To investigate the molecular basis of the Jknull phenotype in the Chinese population, blood samples from Chinese individuals were screened using the 2 mol/L urea solution hemolysis test. Urea and water permeability of erythrocytes membrane was measured by stopped-flow light scattering. Genomic DNA was extracted from lymphocytes. UT-B gene of Jknull's family was analyzed using genomic PCR by primers designed to cover sequences of all exons and exon-intron boundaries in human UT-B gene. One Jknull subject was found from twenty thousand screened Chinese individuals, and it was confirmed that this individual did not express the erythrocyte urea transporter. Genomic sequence analysis of the Jknull individual showed that there were two point mutations, G→C, which is novel, and G→A, at the 3(-acceptor splice site (AG) of intron 5 of UT-B gene. Exon 6 is spliced out in the UT-B transcript due to either of these mutations. Water permeability in Jknull erythrocytes (Pf, ~0.00037 cm/s) was significantly lower than that in normal erythrocytes (Pf, ~0.00062 cm/s) after HgCl2 incubation, providing evidence for UT-B facilitated water transport in human erythrocytes.

关键词： urea transporter JK antigen urea erythrocyte water permeability.

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Arecoline inhibits catecholamine release from perfused rat adrenal gland

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Acta Pharmacologica Sinica 2006年第1期27卷 71-79页

作者： Dong-yoon LIM II-sik KIM department of Pharmacology,College of medicine,Chosun university,Gwangju 501-759,Korea department of Pharmacology College of Medicine Chosun University Gwangju Korea

Aim:To study the effect of arecoline,an alkaloid isolated from Areca catechu,onthe secretion of catecholamines (ca) evoked by cholinergic agonists and themembrane depolarizer from isolated perfused rat adrenal ***:Adrenalglands were isolated from male Sprague-Dawley *** adrenal glands wereperfused with Krebs bicarbonate solution by means of a peristaltic *** cacontent of the perfusate was measured directly using the fluorometric ***:Arecoline (0.1-1.0 mmol/L) perfused into an adrenal vein for 60 min pro-duced dose-and time-dependent inhibition in ca secretory responses evoked byacetylcholine (ACh) (5.32 mmol/L),1.1-dimethyl-4-phenyl piperazinium iodide(DMPP) (100μmol/L for 2 min) and 3-(m-choloro-phenyl-carbamoyl-oxy)-2-butynyltrimethyl ammonium chloride (McN-A-343)(100μmol/L for 2 min).However,lowerdoses of arecoline did not affect ca secretion of high K~+(56 mmol/L);higherdoses greatly reduced ca secretion of high K~+.Arecoline also failed to affectbasal catecholamine ***,in adrenal glands loaded with arecoline(0.3 mmol/L),ca secretory response evoked by Bay-K-8644 (10μmol/L),an activa-tor of L-type ca2+channels,was markedly inhibited,whereas ca secretion bycyclopiazonic acid(10μmol/L),an inhibitor of cytoplasmic ca2+-ATPase,was *** (30μmol/L),which was perfused into the adrenal gland for 60min,however,initially enhanced ACh-evoked ca secretory *** timeelapsed,these responses became more inhibited,whereas the initially enhancedhigh K~+-evoked ca release *** secretion evoked by DMPP and McN-A-343 was significantly depressed in the presence of ***:Arecoline dose-dependently inhibits ca secretion from isolated perfused rat ad-renal gland evoked by activation of cholinergic *** lower doses arecolinedoes not inhibit ca secretion through membrane depolarization,but at largerdoses it *** inhibitory effect of arecoline may be mediated by blocking thecalcium inf

关键词： arecoline catecholamine secretion adrenal gland nicotine

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On linking climate to Chinese dynastic change: Spatial and temporal variations of monsoonal rain

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Chinese Science Bulletin 2010年第1期55卷 77-83页

作者： ZHANG DeEr1, LI Hong-Chun2,3, KU Teh-Lung2,4 & LU LongHua5 1 National Climate Center, Beijing 100081, China 2 department of Earth Sciences & Research Center of Ocean Environment and Technology, Cheng-Kung university, Tainan 70101, Taiwan, China 3 School of Geographical Sciences, Southwest university of China, Chongqing 400715, China 4 department of Earth Sciences, university of Southern california, Los Angeles, ca 90089, usa 5 Chinese Academy of Meteorological Sciences, Beijing 100081, China National Climate Center Beijing China department of Earth Sciences & Research Center of Ocean Environment and Technology Cheng-Kung University Tainan Taiwan China School of Geographical Sciences Southwest University of China Chongqing China department of Earth Sciences University of Southern California Los Angeles USA Chinese Academy of Meteorological Sciences Beijing China

The causal correlation or linkage between the East Asian Monsoon (EAM) intensity and rise/fall of Chinese dynasties recently proposed by high-resolution paleoclimate reconstructions is examined in detail. Aside from many exceptions to the correlation, both instrumental and historical climate records show strong spatial variations of rainfall on annual-to-decadal scales over eastern China. The relationship between rainfall and EAM intensity also exhibits regional disparity. These observations suggest: (1) Tak-ing paleo-proxy records from a single locality in eastern China to imply changes in drought/wetness as to affect the cultural and political history of China is fraught with uncertainty. (2) On annual-to-decadal scales, the thesis that δ18O in speleothems can be used as a proxy for the EAM strength lacks empirical underpinnings.

关键词：古气候重建东亚季风强降雨时空变化中国东部地区连接 EAM 高分辨率

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Regulation of microtubule organization by γ-tubulin-interacting proteins in plant cells

Regulation of microtubule organization by γ-tubulin-interac...

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2009中国植物学会植物细胞生物学学术年会

作者： Bo Liu~* department of Plant Biology,university of california,Davis,ca 95616,usa

γ-Tubulin plays an essential role in microtubule nucleation and organization in plant *** forms a complex with a group of related proteins known as Gamma-tubulin Complex Proteins(GCPs).The function ofγ-tubulin is de... 详细信息

关键词： γ-tubulin microtubule lethal phenotype

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A Spectral Index for Estimating Soil Salinity in the Yellow River Delta Region of China Using EO-1 Hyperion Data

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Pedosphere 2010年第3期20卷 378-388页

作者： WENG Yong-Ling GONG Peng ZHU Zhi-Liang department of Surveying and Mapping Engineering College of Transportation Southeast University Nanjing 210096(China) State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Applications of the Chinese Academy ofSciences and Beijing Normal University Beijing 100101 (China) department of Environment Science Policy and Management University of California Berkeley CA 94720 (USA) EROS Data Center U.S. Geological Survey Sioux Falls SD 57198 (USA)

Soil salinization is one of the most common land degradation processes. In this study, spectral measurements of saline soil samples collected from the Yellow River Delta region of China were conducted in laboratory and hyperspectral data were acquired from an EO-1 Hyperion sensor to quantitatively map soil salinity in the region. A soil salinity spectral index (SSI) was constructed from continuum-removed reflectance (CR-reflectance) at 2052 and 2203 nm, to analyze the spectral absorption features of the salt-affected soils. There existed a strong correlation (r = 0.91) between the SSI and soil salt content (SSC). Then, a model for estimation of SSC with SSI was established using univariate regression and validation of the model yielded a root mean square error (RMSE) of 0.986 and an R2 of 0.873. The model was applied to a Hyperion reflectance image on a pixel-by-pixel basis and the resulting quantitative salinity map was validated successfully with RMSE = 1.921 and R2 = 0.627. These suggested that the satellite hyperspectral data had the potential for predicting SSC in a large area.

关键词： hyperspectral reflectance soil salt content spectral absorption features

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Doxepin protects cultured neurons against oxidative stress-induced injury

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Acta Pharmacologica Sinica 2004年第3期25卷 43-46页

作者： Bian-sheng JI,He JI1,Guo-qing LIU2 department of Pharmacology,China Pharmaceutical university,Nanjing 210009 1department of medicine,Henan university,Kaifeng 475001,China department of Pharmacology China Pharmaceutical University Nanjing 210009 department of Mecine Henan University Kaifeng 475001

AIM: To investigate protective effects of doxepin (Dox) on cultured neuronal injury induced by oxidative stress. METHODS: Cytotoxicities of glutamate (Glu 0.5 mmol/L, 15 min), sodium dithionite (0.5 mmol/L, 24 h) or hemo- globin (Hb100 mg/L,24 h)and the protective effects of Dox were observed. RESULTS: Exposure of cultured neurons to Glu, sodium dithionite and Hb developed a neurotoxicity expressed in the thiazol blue tetrazolium bro- mide (MTT) assay, the increase of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and intracellular [ca2+]i accumulation, as well as the decrease of superoxide dismutase (SOD) activity. DOX 1-100 nmol/L significantly inhibited all above changes. CONCLUSION: Dox protects cultured neurons against oxidative stress-induced injury by suppressing intracellular [ca2+]iaccumulation, decreasing lipid peroxide generation and stimulating antioxidant enzyme.

关键词： doxepin ischemia glutamic acid hemoglobins neurons

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ca2+ sparks and ca2+ glows in superior cervical ganglion neurons

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Acta Pharmacologica Sinica 2006年第7期27卷 848-852页

作者： Li-jun YAO~(2,3,4) Gang WANG~(3,4) Kun-fu OU-YANG~3 Chao-liang WEI~3 Xian-hua WANG~3 Shi-rong WANG~3 Wei YAO~3 Hong-ping HUANG~3 Jian-hong LUO~2 cai-hong WU~3 Jie LIU~3 Zhuan ZHOU(3,5) He-ping CHENG~(3,5)2 department of Neurobiology,Zhejiang university School of medicine,Hangzhou 310031,China 3 Institute of Molecular medicine and State Key Laboratory of Biomembrane Engineering,Peking university,Beijing 100871,China department of Neurobiology Zhejiang University School of Medicine Hangzhou China Institute of Molecular medicine and State Key Laboratory of Biomembrane Engineering Peking University Beijing China

Aim:ca2+release from the endoplasmic reticulum（ER）is an integral component ofneuronal ca2+*** present study is to investigate properties of localca2+release events in superior cervical ganglion（SCG）***:Pri-mary cultured SCG neurons were prepared from neonatal rats（P3-P7）.Low con-centration of caffeine was used to induce ca2+release from the ER ca2+store,andintracellular ca2+was recorded by high-resolution line scan confocal imaging andthe ca2+indicator ***:Two populations of local ca2+release eventswith distinct temporal characteristics were evoked by 1.5 mmol/L caffeine near thesurface membrane in the soma and the neurites of SCG *** eventssimilar to classic ca2+sparks lasted a few hundreds of milliseconds,whereas long-lasting events displayed duration up to tens of *** somatic andneurite sparks were of 0.3-and 0.52-fold increase in local Fluo-4 fluorescence,*** ca2+glows were brighter（ΔF/F0approximately 0.6）,butwere highly confined in *** half maximum of full duration of neurite sparkswas much longer than those in the soma（685 vs 381 ms）.Conclusion:Co-exist-ence of ca2+sparks and ca2+glows in SCG neurons indicates distinctive localregulation of ca2+release *** local ca2+signals of variable,site-specifictemporal length may bear important implications in encoding a"memory"of thetrigger signal.

关键词： superior cervical ganglion neurons ca2+ sparks ryanodine receptors caffeine

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Identification and Characterization of a Rat Novel Gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4, and Nai-He JING1* 1 Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China School of Life Science and Biotechnology, 2 Shanghai Jiaotong university, Shanghai 200030, China Institute of Neurobiology, Fudan university, Shanghai 200433, China 3 4 department of Cell Biology, Neurobiology and Anatomy, College of medicine, university of Cincinnati, Cincinnati, Ohio, usa Laboratory of Molecular Cell Biology Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China School of Life Science and Biotechnology Shanghai Jiaotong UniversityShanghai 200030 China Institute of Neurobiology Fudan UniversityShanghai 200433 China department of Cell Biology Neurobiology and Anatomy College of Medicine University of CincinnatiCincinnati Ohio USA

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the ca1, ca2, ca3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

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QUALIFIcaTION OF UNCERTAINTY FOR SIMULATING SOLUTE TRANSPORT IN THE HETEROGENEOUS MEDIA WITH SPARSE GRID COLLOcaTION METHOD

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Journal of Hydrodynamics 2009年第6期21卷 779-789页

作者： SHI Liang-sheng YANG Jin-zhong State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan University Wuhan 430072 China The Sonny Astani department of Civil and Environmental Engineering University of Southern California Los Angeles CA 90089 USA

The sparse grid collocation method is discussed to qualify the uncertainty of solute transport. The Karhunen-Loeve （KL） expansion is employed to decompose the log transformed hydraulic conductivity. The head, velocity and concentration fields are represented by the Lagrange polynomial expansion. A sparse grid collocation method is then used to reduce the original stochastic partial differential equations to a set of deterministic equations which is collocated at selected interpolation （collocation） points. The collocation points are constructed by the Smolyak algorithm. The accuracy, efficiency and convergence property of sparse grid collocation method are investigated by numerical experiments. The analysis shows that stochastic collocation strategy helps to decouple stochastic computations, and all the numerical computation is possible to be implemented by existing deterministic finite element codes. The proposed method provides an efficient way to evaluate the uncertainty of the solute transport in the heterogeneous media.

关键词： stochastic partial differential equations collocation method solute transport uncertainty

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Prevalence of Helicobacter pylori infection among patients with esophageal carcinoma

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World Journal of Gastroenterology 2024年第29期30卷 3479-3487页

作者： Miriam López-Gómez Maria Morales Rebeca Fuerte Marta Muñoz Pedro-David Delgado-López Jorge francisco Gómez-Cerezo Enrique casado department of Medical Oncology Precision Oncology LaboratoryInfanta Sofía University HospitalSan Sebastián de los Reyes 28231MadridSpain department of Medical Oncology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain department of Internal medicine Infanta Sofía University HospitalSan Sebastián de los Reyes 28703MadridSpain department of Pathology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain department of Neurosurgery Burgos University HospitalBurgos 09006Spain department of Internal medicine Infanta Sofía University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain department of Medical Oncology Infanta Sofia University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain

BACKGROUND Helicobacter pylori(***)is a widespread microorganism related to gastric adenocarcinoma(AC).In contrast,it has been reported that an inverse association exists between *** infection and esophageal *** mechanisms underlying this supposedly protective effect remain *** To determine the prevalence of *** infection in esophageal carcinoma patients,we performed a retrospective observational study of esophageal tumors diagnosed in our *** We retrospectively reviewed the prevalence of *** infection in a cohort of patients diagnosed with esophageal *** or previous proton pump inhibitor(PPI)usage was also *** A total of 89 patients with esophageal carcinoma(69 males,77.5%),with a mean age of 66 years(range,26-93 years)were *** was the most frequent pathological variant(n=47,52.8%),followed by squamous cell carcinoma(n=37,41.6%).Fourteen ACs(29.8%)originated in the gastroesophageal junction and 33(70.2%)in the esophageal ***,54 patients(60.7%)presented at stages III and *** *** infection occurred only in 4 patients(4.5%),3 with AC(6.3%of all ACs)and 1 with squamous cell carcinoma(2.7%of all squamous cell tumors).All patients with previous *** infection had stage *** one patient had received prior *** eradication therapy,whereas 86(96.6%)had received previous or concomitant PPI *** In our cohort of patients,and after histologic evaluation of paraffin-embedded primary tumors,we found a very low prevalence of previous *** *** also reviewed the medical history of the patients,concluding that the majority had received or were on PPI *** minimal prevalence of *** infection found in this cohort of patients with esophageal carcinoma suggests a protective role.

关键词： Helicobacter pylori Eradication Esophageal tumor Dysbiosis Proton pump inhibitors carcinogenesis Microbiota Incidence

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