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CLONING PROMOTER OF HUMAN SATBl GENE AND EFFECT OF ATRA AND CoCl_2 ON ITS ACTIVITY

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Journal of Shanghai Second medical university(Foreign Language Edition) 2006年第1期18卷 1-6页

作者：李颖戴冰冰蔡蓉仇全于丽莉卢健 department of biochemistry & molecular biology

Objective To explore the structure and activity of SATB1 promoter in different cells, ATRA and CoCl2 effect on its activity. Methods Using luciferase system to assay the promoter activity of human SATB1 gene, three luciferase reporter vectors were constructed which driven by different regions of 5＇ untranslated sequence from human SATB1 gene , called pGL3-SP2946-luc , pGL3-SP1718-luc and pGL3-SP751-luc , and transfected into Jurkat T, K562, U937 and Hela cells transiently using lipofectinamine, the expression activity was detected at different dosage of A TRA and CoCl2treatment for different time course. Results The reporter gene expression from SATB1 promoter were high activity in U937 cell, moderate in Jurkat T cell, low activity in K562 cell and showed no obvious activity in Hela cell, the reporter gene expression from pGL3-SP751-1uc kept on the higher lever in Jurkat T, K562 and U937 cells than the other two vectors. We also found that the repressive effect of CoCI2 on SATBI＇s mRNA expression and the relative luciferase expression from pGL3-SP751-luc in U937 cell was down-regu- lated obviously by ATRA and CoCI2 in the concentration- and time-dependent manners. Conclusion SATB1 pro- rooter drives gene expression with cell-specificity and its core promoter region maybe exist in the - 751 - - 9bp of 5＇ untranslated region of human SATB1 gene. Combined with the experiment result we found before that SATB1 was down-regulated by ATRA in U937, the results imply that STAB1 maybe is down-regulated by ATRA and CoCl2 through its promoter in the differentiation of myeloid cell line-U937.

关键词： SATB1 promoter ATRA CoCl2

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转基因内皮细胞tpA纤溶活性的变化

上海医科大学学报 1997年第6期24卷 450-452页

作者：刘鹏彭鲁英韩琴琴宋后燕上海市心血管病研究所生物化学研究室上海医科大学中山医院心内科

利用基因重组技术，将tpAcDNA与逆转录病毒载体LNSX重组构成LNS－ipA，转染病毒包装细胞，形成的重组逆转录病毒颗粒再用来感染牛血管内皮细胞。经G418筛选后的单克隆细胞培液，分别经纤维蛋白板法和发包底物法测取活性。结果：经基因... 详细信息

利用基因重组技术，将tpAcDNA与逆转录病毒载体LNSX重组构成LNS－ipA，转染病毒包装细胞，形成的重组逆转录病毒颗粒再用来感染牛血管内皮细胞。经G418筛选后的单克隆细胞培液，分别经纤维蛋白板法和发包底物法测取活性。结果：经基因转染的PA317细胞和牛血管内皮细胞，在含人纤维蛋白原和凝血酶的纤维蛋白板上出现溶圈；随培养时间的延长，细胞数量增加，tpA分泌活性也增加。结论：含人tpAcDNA转录单位的牛血管内皮细胞，其纤溶活性明显增高。该研究为将来进行基因治疗奠定了基础。

关键词：组织型纤溶酶原激活剂内皮细胞血栓形成

Different neurotropic pathogens elicit neurotoxic CCR9-or neurosupportive CXCR3-expressing microgli

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Different neurotropic pathogens elicit neurotoxic CCR9-or ne...

Cartilage oligomeric matrix protein,a novel N-terminus binding protein of angiotensin Ⅱ type 1 receptor,inhibits aortic aneurysm formation

中国免疫学会第五届全国代表大会暨学术会议

作者： Zhou Gang, He Yu-ling, He Li, Xie Luo-kun , Xiong Jie, Lei Hao, Wei Li, Hu Chun-song, Liu Jun-yan, Jian Ming-shen, Jin You-xin, Gong Fei-li, Jin Bo-quan, Tan Jin-quan departments of Immunology Parasitology, Institute of Allergy and Immune-related Diseases Laboratories of Neuroscience Allergy and Clinical Immunology, Center for medical Research, Wuhan university school of Medicine, Wuhan The State Key Laboratory of molecular biology and Neuroscience, Institute of biochemistry and Cell biology, Shanghai Institutes for Biological sciences, Chinese Academy of science, Shanghai The State Key Laboratory of Magnetic Resonance and Atomic and molecular Physics, Institute of Physics and Mathematics, Chinese Academy of sciences, Wuhan Key Laboratory of Colloid, Interface science and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of sciences, beijing department of Immunology, College of basic medical sciences, Anhui medical university, Hefei department of Immunology, Tongji medical College, Huazhong university of science and Technology, Wuhan department of Immunology, Fourth Military medical university, Xian, P. R. china

What mechanism to determine microglia accomplishing destructive or constructive role in CNS remains nebulous. We report here that intracranial priming and re-challenging with T. gondii in mice elicit neurotoxic CCR9+ Irg1+(immunoresponsive gene 1) microglia, which are resistant to apoptosis and produce high level of TNF-α;priming and re-challenging with LCMV (lymphocytic choriomeningitis virus) elicit neurosupportive CX-CR3+ Irg1- microglia, which are sensitive to apoptosis and produce high level of IL-10 and TGF-β. Administration of CCR9 or/and Irgl siRNAs alters the frequency and functional profiles of neurotoxic CCR9+ Irg1+ and neurosupportive CXCR3 +Irg1- microglia in vivo. Moreover, by using a series of different neurotropic pathogens including intracellular parasites, chronic virus, bacteria, toxic substances, and CNS injury to intracranially prime and subsequent re-challenge mice, the bi-directional elicitation of microglia has been confirmed as neurotoxic CCR9+ Irg1+ and neurosupportive CXCR3+ Irg1- cells in these mouse models. These data suggest that there exist two different types of microglia, providing with a novel insight into microglial involvement in neurodegenerative and neuroinflam-matory pathogenesis such as Alzheimer s disease and AIDS dementia.

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来源： cnki会议评论

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中国病理生理杂志 2012年第11期 2049-2049页

作者： HUANG Ya-qian,ZHENG Jin-gang,LIU Bo,XIE Jin-sheng,WANG Wen-gong,GUO Wei,JIA Xin,ZHANG Tao,LIU Zi-yi,ZHANG Heng,TANG Chao-shu,ZHU Yi,XU Qing-bo,WANG Xian,KONG Wei(department of Physiology and Pathophysiology,school of basic medical sciences,peking university,Key Laboratory of molecular Cardiovascular science,Ministry of Education,beijing 100191,china)

Abdominal aortic aneurysm(AAA)is a life-threatening cardiovascular *** endogenous molecules that modulate the aneurism process remain *** oligomeric matrix protein(COMP)is a matricellular glycoprotein highly expressed... 详细信息

关键词： type 1 receptor,inhibits aortic aneurysm formation

Antifertility Effects of Orally Administration of Low Dose Gossypol Acetic Acid Combined with Methyltestosterone Plus Ethinyl Estradiol on Male Rat

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Journal of Reproduction and Contraception 2008年第4期19卷 201-210页

作者： Zhanjun YANG Wei-san YE Lan WANG Yan GUO She-pu XUE department of Human Anatomy BaoTou Medical College Science and Technology University Inner Mongolia Baotou 014010 China department of Histology and Embryology School of Basic Medicine Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100005 China

Objective To investigate the feasibility and optimal regimen of orally administration of low dose gossypol acetic acid （GA） combined with methyltestosterone （MT） plus ethinyl estradiol （EE） for contraception in males.Methods Wistar male rats were randomly assigned into four groups, 20 in each group. Animals in group A or B were administered daily with 1% methyl cellulose or GA （12 mg/kg） suspended in 1% methyl cellulose, respectively. Rats in group C or D took firstly GA 12 mg/kg＋MT 20 mg/kg＋EE 0.1 mg/kg or MT 20 mg/kg＋EE 0.1 mg/kg, in asuspension with 1% methyl cellulose, via gastric intubation. After the infertilities were initiated（6 weeks for group C, 8 weeks for group D）, GA was served alone while MT＋EE were withdrawn in rats of groups C and D. The treatment was ceased after 18 weeks and some males from group C were permitted to recover. Fertility testing, 10 males per group, was served for determining infertility or restoration of fertility in treated rats. Examinations of histology and biochemistry in treated rats were used to examine the morphologic influences on sperm, testis, epididymides and viscera, and biochemical changes in blood. The growth and development of F1 generation of the rats would also be tested in a series of behavioral tests.Results Ten rats from group C were infertile at week 6 after treatment, and the fulfilled infertility was maintained with low-dose GA （12 mg/kg） only daily. Six weeks after cessation of treatment, all of treated males recovered their fertility. However, 8 of 10 rats from group D were in sterility at 6th week of treatment and all at 8th week of treatment, but the infertility could not be kept with the similar dose GA alone later on. Moreover, no adverse effects were found in our present experiments.Conclusion Administration of oral low dose GA combined with MT and EE as loading dose could successfully induce infertility in short term, whereafter the efficacy could completely be maintained by similar low dose of

关键词： gossypol methyltestosterone ethinyl estradiol antifertility effect rats

小鼠PD-1基因启动子克隆鉴定及转录调控分析

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生物化学与生物物理进展 2010年第5期37卷 527-533页

作者：郑花鸯汤必奎王培伟高方健胡晓波龚立朱乃硕复旦大学生命科学学院微生物感染与分子免疫学实验室遗传学国家重点实验室生物医学研究院上海200433 蚌埠医学院生物科学系蚌埠233030

PD-1分子是一种重要的免疫调控因子,目前对其自身表达调控尚未有系统的研究.对PD-1启动子区域进行分析,克隆构建了含PD-1基因上游约2kb范围内含4种不同长度调控序列的双荧光素酶表达载体.通过FACS检测发现,小鼠T淋巴瘤EL4细胞稳定表达PD... 详细信息

PD-1分子是一种重要的免疫调控因子,目前对其自身表达调控尚未有系统的研究.对PD-1启动子区域进行分析,克隆构建了含PD-1基因上游约2kb范围内含4种不同长度调控序列的双荧光素酶表达载体.通过FACS检测发现,小鼠T淋巴瘤EL4细胞稳定表达PD-1,而骨髓瘤Sp2/0-Ag细胞则在佛波酯(phorbol 12-myristate 13-acetate,PMA)和离子霉素(ionomycin,IO)诱导后才表达PD-1.4种双荧光素酶报告系统在上述两种细胞中检测PD-1启动子各区段活性显示,-227～+49bp区域含有PD-1核心启动子,上游-1127～-716bp含有较强正性调节元件,而在-1685～-1128bp、-715～-228bp两个区域含有负性调节元件,这种正负调控区交错的启动子活性现象说明了PD-1基因表达调控的复杂性,这些结果为PD-1基因的表达调控提供了结构基础和依据.

关键词： PD-1 启动子鉴定转录调控分析

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醛糖还原酶的分离纯化和性质分析

上海医科大学学报 1996年第6期23卷 427-430页

作者：刘长山陈惠黎查锡良朱禧星上海医科大学华山医院内分泌科山东潍坊人民医院内分泌科

利用离心、盐析及ＤＥＡＥ－纤维素、羟基磷灰石、葡聚糖－Ｇ１００多步层析法将牛睾丸ＡＲ分离纯化，醛糖还原酶（ａｌｄｏｓｅｒｅｄｕｃｔａｓｅ，ＡＲ），并利用体外酶动力学方法测定其底物特异性，为寻找有效的醛糖还原酶抑制剂提... 详细信息

利用离心、盐析及ＤＥＡＥ－纤维素、羟基磷灰石、葡聚糖－Ｇ１００多步层析法将牛睾丸ＡＲ分离纯化，醛糖还原酶（ａｌｄｏｓｅｒｅｄｕｃｔａｓｅ，ＡＲ），并利用体外酶动力学方法测定其底物特异性，为寻找有效的醛糖还原酶抑制剂提供线索。结果：纯化的酶经ＳＤＳ－ｐＡＧＥ电泳鉴定示一单一蛋白条带，牛睾丸ＡＲＭｒ约为（４００００±１０００）ｕ，ＡＲ底物特异性，按１／ｋｍ大小依次排列为：Ｐ－硝基苯醛，ＤＬ－甘油醛，Ｄ－葡萄糖醛酸，Ｄ－木糖，Ｄ－半乳糖，Ｄ－葡萄糖。

关键词：醛糖还原酶睾丸纯化分离

大鼠肝抑素纯化及其对再生肝细胞和肝癌细胞增殖的影响

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上海医科大学学报 1997年第1期24卷 7-10页

作者：孙亚平成令忠刘银坤朱继红张夏英顾云娣中国科学院上海细胞生物学研究所 20031 上海医科大学基础医学院组织胚胎学教研室 200032 上海医科大学基础医学院生物化学教研室200032

制备大鼠肝抑素（ＨＣ）纯品，研究它对再生肝细胞和肝癌细胞体外增殖的影响。用ＤＥＡＥ－Ｃｅｌｌｕｌｏｓｅ阴离子交换层析法提取ＨＣ纯品，用液法检测ＨＣ样品的生物活性。结果：提取的ＨＣ纯品的ＳＤＳ电泳纯度达８９．５％，Ｍｒ... 详细信息

制备大鼠肝抑素（ＨＣ）纯品，研究它对再生肝细胞和肝癌细胞体外增殖的影响。用ＤＥＡＥ－Ｃｅｌｌｕｌｏｓｅ阴离子交换层析法提取ＨＣ纯品，用液法检测ＨＣ样品的生物活性。结果：提取的ＨＣ纯品的ＳＤＳ电泳纯度达８９．５％，Ｍｒ１３．５ × １０３；该ＨＣ纯品对大鼠再生肝细胞和人肝癌细胞体外增殖有明显抑制作用，抑制率分别为６３．１％（０．２ｍｇ／Ｌ）和７７．７％（０．３ｍｇ／Ｌ），但对人胃癌细胞增殖无抑制效应。结论：结果提示提取的ＨＣ是抑制再生肝细胞和肝癌细胞增殖的特异性因子。

关键词：肝肿瘤肝细胞肝癌细胞肝抑素纯化

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中国肝癌筛查卫生经济学研究的系统评价

中国循证医学杂志 2018年第5期18卷 442-449页

作者：白方舟王乐王宇婷黄慧瑶刘成成朱娟任建松赵建军韩优莉曲春枫代敏石菊芳国家癌症中心/中国医学科学院北京协和医学院肿瘤医院癌症早诊早治办公室北京100021 国家癌症中心/中国医学科学院北京协和医学院肿瘤医院免疫学研究室/分子肿瘤学国家重点实验室北京100021 国家癌症中心/中国医学科学院北京协和医学院肿瘤医院肝胆外科北京100021 首都医科大学卫生管理与教育学院北京100069

目的系统评价中国人群肝癌筛查卫生经济学研究现状,为后续进一步开展相关研究提供依据。方法计算机检索Pub Med、EMbase、The Cochrane Library、Web of science、CNKI、Wan Fang Data、CBM和VIP数据库,搜集我国人群肝癌筛查卫生经济学... 详细信息

目的系统评价中国人群肝癌筛查卫生经济学研究现状,为后续进一步开展相关研究提供依据。方法计算机检索Pub Med、EMbase、The Cochrane Library、Web of science、CNKI、Wan Fang Data、CBM和VIP数据库,搜集我国人群肝癌筛查卫生经济学研究,检索时限均从建库至2017年12月31日。由2位研究者独立筛选文献、提取资料,对纳入研究基本特征、卫生经济学评价方法及主要结果等进行描述性分析,并对研究质量及报告规范性进行评价。结果共纳入5个研究。纳入研究的肝癌筛查起始年龄在35~45岁,筛查技术多选用甲胎蛋白(AFP)联合超声,频率以每半年1次居多。5个研究整体质量尚可;整体报告规范性尚可,得分中位数为78%(60%,78%)。2个研究报道了基于每检出1例肝癌的成本,分别为4.4万元和57.5万元。3个研究报道了基于挽救生命年(LYS)和质量调整生命年(QALY)的成本-效果比(CER)指标,仅1个中国内地研究报道了每挽救一个LYS的成本,为1 775元,其与当地人均国内生产总值(GDP)比值为0.1;另2个我国台湾地区的研究报道了4组筛查的成本-效果比,其与当地人均GDP比值范围为1.0~2.2。结论肝癌高发地区我国台湾地区研究提示在普通人群中开展肝癌筛查经济有效;而我国大陆研究提示在高危人群中开展AFP联合超声筛查经济有效,但仅有1个地区性研究支持,仍需更多基于随机对照试验或队列研究的卫生经济学评价予以验证。

关键词：肝癌筛查卫生经济学评价成本-效果分析系统评价中国