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Research on and Control of Trypanosoma evansi Infection in Equines in china

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Research on and Control of Trypanosoma evansi Infection in E...

Nanostructured Sulfide Composite Coating Prepared by Atmospheric Plasma Spraying

中国动物学会第七届全国青年寄生虫学工作者学术讨论会

作者： Suo Xun Kong Fanyao(Parasitology Laboratory,college of veterinary medicine,china agricultural university,beijing 100094,china)

This review briefly describes some major aspects concerning research conducted mainly from 1980-2001 on and control of Trypanosoma evansi in equines in *** ResearchHere is only a brief list of research *** Zhong et al(1996) reported that with 5% dimethyl sulfoxide, 96% of *** survived for 712 days in liquid nitrogen. The result of Bai et al (1993) was 574200 days. Xu(1991) could preserve *** for 1 year under-80℃ condition.

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材料工程 2006年第Z1期34卷 281-284页

作者： GUAN Yao-hui~1,XU Yang~1,ZHENG Zhong-yu~2,TONG Xiao-hui~2(1 Engineering college of china agricultural university,beijing 100083,china 2 beijing Research Institute of Mechanical & Electrical Technology,beijing 100083,china) Engineering college China Agricultural University Beijing 100083 China beijing Research Institute of Mechanical and Electrical Technology Beijing 100083 China

Nanostructured FeS-SiC coating was deposited by atmospheric plasma spraying（APS）.The microstructure and phase composition of the coating were characterized with SEM and XRD,*** addition,the size distribution of the reconstituted powders and the porosity of the coating have been *** was found that the reconstitiuted powers with sizes in the range of 20 to 80 μm had excellent flowability and were suitable for plasma spraying *** as-sprayed FeS-SiC composite coating exhibited a bimodal distribution with small grains（30～80nm） and large grains（100～200nm）.The coating was mainly composed of FeS and SiC,a small quantity of Fe1-xS and oxide were also *** porosity of the coating was approximately 19%.

关键词： plasma spraying nanostructured FeS-SiC composite coating

Effects of Carbon Dioxide on Astringency Removal in Mopanshi Persimmon

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agricultural Sciences in china 2003年第12期2卷 1382-1386页

作者： LENG Ping, LI Bao, ZHANG Wen and JIA Ke-gong (college of agricultural and Biotechnology, china agricultural university, beijing 100094, P. R. china) college of agricultural and Biotechnology China Agricultural UniversityBeijing 100094P.R.China

Fruits of persimmon (Diospyros kaki cv. Mopanshi) were used to investigate the effects of different concentrations of carbon dioxide (CO 2) gas on removing astringency after harvest. Treatment of 95% concentration of CO 2 gas gave the best results; fruits turned non-astringent after 20 h, and kept the fruit firm for 7 d at room temperature. Fruits, treated with 85 and 90% concentration of CO 2 gas, turned non-astringent after 2428 h, and the firmness-keeping stage was 56 d at room temperature. While the CO 2 gas was at 80, 70 and 60%, the de-astringency period was 48, 72 and 96 h, and the corresponding firmness-keeping stage was 3, 2 and 1 d, respectively. Fruits, treated with 50% concentration of CO 2 gas, remained astringent.

关键词： Persimmon Carbon dioxide Astringency removal Firmness-keeping

Study on Community Diversity of Coastal Shelter Forest in Jiaonan

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Chinese Journal of Population,Resources and Environment 2005年第3期3卷 42-47页

作者： Xu Jingwei 1,2, Li Chuanrong 3, Li Qi 4, Zheng Li 3, & Wang Weidong 1 1 beijing Forestry university,beijing 100871,china 2 Shandong Acdemy of Forestry, Jinan 250014, china 3 college of Forestry, Shandong agricultural university, Taian 271018, china 4 Shandong Normal university, Jinan 250014, china beijing Forestry university Beijing 100871 China Shandong Acdemy of Forestry Jinan 250014 China college of Forestry Shandong Agricultural University Tatan 271018 China Shandong Normal university Jinan 250014 China

Based on the research of the plots in coastal areas in Jiaonan city, Shandong Province, the indices of richness, evenness and diversity of different community types are analyzed. The results show that: The richness indices indicates that arbor layers are close to shrub layers, and both are lower than herbage layers; the ranges among communities vary a little except those of the arbor ones. Mostly the biodiversity indices reveal that herbage, the arbor, the shrub; Biodiversity of communities has great influences on microclimate to a certain extent and is under the control of regional environment.

关键词： coastalshelterforest richness biodiversity index microclimate

Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2) Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,beijing 100052,china 2 School of medicine,Xi'an Jiaotong university,Xi'an 710061,china 3 National Laboratory of Medical Molecular Biology,Institute of Basic Medical Science,Chinese Academy of Medical Sciences and Peking Union Medical college,beijing 100005,china 4 college of Science and veterinary medicine,Northwest Agriculture and Forest university,Yangling 712100,china State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052 China School of medicine Xi'an Jiaotong UniversityXi'an 710061 China National Laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005 China college of Science and veterinary medicine Northwest Agriculture and Forest UniversityYangling 712100 China

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 23-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes

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Journal of Integrative Plant Biology 2009年第8期51卷 751-761页

作者： Liming Zhou1,2, Ying Fu1,2 and Zhenbiao Yang2,3 (1State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, college of Biological Sciences, china agricultural university, beijing 100193, china 2china agricultural university (CAU)-university of California, Riverside (UCR) Joint Center for Biological Sciences and Biotechnology, college of Biological Sciences, china agricultural university, beijing 100193, china 3Center for Plant Cell Biology and Department of Botany and Plant Sciences, university of California, Riverside, CA 92521, USA) State Key Laboratory of Plant Physiology and Biochemistry Department of Plant Sciences College of Biological Sciences China Agricultural University Beijing 100193 China china agricultural university (CAU)-university of California Riverside (UCR) Joint Center for Biological Sciences and Biotechnology College of Biological Sciences China Agricultural University Beijing 100193 China Center for Plant Cell Biology and Department of Botany and Plant Sciences University of California Riverside CA 92521 USA)

Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. *** members of calrnodulin （CAM）, CaM-like （CML）, calcium-dependent protein kinase （CDPK） and calcineurin B-like protein （CBL） families were tagged with green fluorescence protein （GFP） and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane （PM）, and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.

关键词： Arabidopsis calcium sensor calcium signaling Rho-related GTPase from plants subcellular localization tip growth

Effects of Light and Temperature on Uptake andDistribution of Nitrogen, Phosphorus and Potassiumof Cucumber in Solar Greenhouse

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agricultural Sciences in china 2002年第11期1卷 1246-1250页

作者： PEI Xiao-bo, ZHANG Fu-man and WANG Liu(college of Horticulture, china agricultural university , beijing 100094 , P. R. china) college of Horticulture China Agricultural University Beijing 100094P.R.China

The influence of light and temperature conditions inside solar greenhouse of winter-spring and autumn-winter crop in northern china on uptake and distribution of nitrogen. phosphorus and potassium of cucumber was studied. The results showed that plant root development and uptake and distribution of N, P and K benefited more from inside light and temperature changes in winter-spring crop. Root volume and root activity increased more rapidly in winter-spring than in autumn-winter. Uptake of total N, P2O5 and K2O increased with plant development in winter-spring, and declined in autumn-winter crop. Distribution of total N, P2O5 and K2O at different part of cucumber at fruit bearing stage was significantly influenced by inside light and temperature of solar greenhouse. Total N, P2O5 and K2O were mainly distributed to leaves and stems at early stage, and increasingly to fruits after fruit bearing.

关键词： Cucumber Solar greenhouse Light and temperature conditions Nutrient Uptake Distribu-tion

Nitric oxide produced by cumulus cells stimulates maturation of mouse oocytes

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Chinese Science Bulletin 2003年第1期48卷 72-75页

作者： BU Shumin, XIA Guoliang, XIE Huirong & GUO Yong college of Biological Science, china agricultural university, beijing 100094, china Correspondence should be addressed to Xia Guoliang (e-mail: glxia@ mail.cau. ***) college of Biological Science China Agricultural University Beijing 100094 China

The effect of sodium nitroprusside (SNP), a donor of nitric oxide, on meiotic maturation of mouse oo-cytes was studied by injecting Nw-nitro-L-arginine methyl ester (L-NAME) intra-peritoneal (ip), a nitric oxide synthase inhibitor, or culturing oocytes in the medium supplemented with L-NAME or hypoxanthine (HX) to arrest the sponta-neous oocyte maturation in vitro. The results showed that the inhibitory effect of L-NAME by injecting 10 mg/kg ip on extrusion of the first polar body only could be reversed by injecting 2.5 mg/kg SNP with L-NAME simultaneously (P 3 mol/L) had no effect on HX-arrested oocyte meiotic maturation. The optimal concentration of SNP on CEOs had no effect on DOs. The dose of 10-3 mol/LL-NAME demonstrated a significant suppression in formation of PB1, but not in GVBD. This inhibition was reversed by the addi-tion of SNP. These results indicated that the physiological levels of NO produced by cumulus cells could stimulate mei-otic maturation of mouse oocytes both in vivo and in vitro.

关键词：卵丘细胞 NO 一氧化氮卵母细胞成熟小白鼠刺激作用

Effect of Weak Light on the Photoassimilates Distribution and Transformation of Young Grape Plants

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agricultural Sciences in china 2002年第9期1卷 1006-1011页

作者： ZHAN Ji-cheng, HUANG Wei-dong and WANG Li-jun(college of Horticultural Sciences , china agricultural university, beijing 100094 , P. R . china) college of Horticultural Sciences China Agricultural University Beijing 100094 P. R. China

The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyu) were studied by shading. Compared to the grape grown under natural light intensity environment, the net photosynthetic rate diurnal variation curve of experimental plants grown in weak light intensity environment was remarkably lower. Its leaf and stem biomass ratio increased when light intensity decreased, the root and new shoot biomass ratio showed an opposite trend. The 14 C-photoassimilates was mostly distributed to young leaves and stem, a little was distributed to root. The metabolism of 14C-photoassimilates distributed to the entire grape body were also changed under weak light intensity environment.

关键词： Weak light Net photosynthetic rate(Pn) Biomass partition Photoassimilates distribution Photoassimilates transformation