检索结果-南通市图书馆

Changes in plant biomass and species composition of alpine Kobresia meadows along altitudinal gradient on the Qinghai-Tibetan Plateau

The First International Conference on Computers and Computing Technologies in agriculture

作者： YONG TIAN~(1 2) LIZHI LU~(2 *) YAN FU~1 DEQIAN WANG~2 GUOQIN LI~2 AIPING YUAN~2 QINGYAN YUAN~2 JIN ZHAO~3 1 Laboratory of Animal Genetics college of Animal sciences zhejiang university 268 Kaixuan Road hangzhou 310029 PR china 2 Institute of Animal Husbandry and Veterinary science zhejiang Academy of Agricultural sciences 198 Shiqiao Road hangzhou 310021 PR china 3 college of life sciences china jiliang university 258 Xueyuan Road hangzhou 310018 PR china

＜正＞The NDRG4 gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase *** protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscle *** map this gene in the chicken chromosome,a 6000 rads chicken-hamster radiation hybrid panel（ChickRH6） was *** were designed according to the published human sequence for amplification of the chicken NDRG4 gene by comparative *** compared the corresponding human mRNA sequence with the predicted coding sequence of the chicken NDRG4 gene,and found that the assembled contig shared a high percentage of similarity with that of the human *** of samples from the ChickRH6 revealed the locations of the NDRG4 gene to be linked to the marker PARD6G（5 cR away）with a LOD score of *** present results support the idea that the RH technique is indeed a powerful tool for mapping genes due to its rapidity,precision, convenience and reproducibility.

关键词： chicken genetic marker mapping NDRG4 gene RH panel

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

science china(life sciences) 2008年第1期51卷 86-94页

作者： WANG ChangTing1,3, CAO GuangMin1, WANG QiLan1, JING ZengChun1, DING LuMing1 & LONG RuiJun2 1 Northwest Institute of Plateau biology, Chinese Academy of sciences, Xining 810001, china 2 college of Pastoral agriculture science and technology, Lanzhou university, Lanzhou 730070, china 3 Graduate university of the Chinese Academy of sciences, Beijing 100039, china 1. Northwest Institute of Plateau biology Chinese Academy of Sciences Xining 810001 China 3. Graduate university of the Chinese Academy of sciences Beijing 100039 China 2. college of Pastoral agriculture science and technology Lanzhou University Lanzhou 730070 China

Alpine Kobresia meadows are major vegetation types on the Qinghai-Tibetan Plateau. There is growing concern over their relationships among biodiversity, productivity and environments. Despite the im-portance of species composition, species richness, the type of different growth forms, and plant bio-mass structure for Kobresia meadow ecosystems, few studies have been focused on the relationship between biomass and environmental gradient in the Kobresia meadow plant communities, particularly in relation to soil moisture and edaphic gradients. We measured the plant species composition, her-baceous litter, aboveground and belowground biomass in three Kobresia meadow plant communities in Haibei Alpine Meadow Ecosystem Research Station from 2001 to 2004. Community differences in plant species composition were reflected in biomass distribution. The total biomass showed a de-crease from 13196.96±719.69 g/m2 in the sedge-dominated K. tibetica swamp to 2869.58±147.52 g/m2 in the forb and sedge dominated K. pygmaea meadow, and to 2153.08±141.95 g/m2 in the forbs and grasses dominated K. humilis along with the increase of altitude. The vertical distribution of below-ground biomass is distinct in the three meadow communities, and the belowground biomass at the depth of 0-10 cm in K. tibetica swamp meadow was significantly higher than that in K. humilis and K. pygmaea meadows (Pbiomass was negatively correlated to species richness (Pbiomass was positively correlated to soil organic matter, soil moisture, and plant cover (Pbiomass was positively correlated with soil moisture (P<0.05). However, in the K. py

关键词： plant species richness plant litter aboveground biomass belowground biomass soil moisture alpine meadow

苏云金芽胞杆菌营养期杀虫蛋白Vip与Cry蛋白的协同作用

在线全文

学校读者我要写书评

暂无评论

微生物学通报 2003年第5期30卷 43-48页

作者：蔡启良刘子铎孙明喻子牛华中农业大学生命科学技术学院教育部暨农业部农业微生物重点实验室武汉430070

将构建的营养期杀虫蛋白基因vip83表达质粒pBMB2 32 8和含杀虫晶体蛋白基因(cry1Ac1 0或cry1Ca)质粒同时电转化无质粒突变株BMB1 71并双抗筛选。经PCR特异引物扩增验证 ,分别得到含cry1Ac1 0和vip83、cry1Ca和vip83的双基因重组菌BMB2 8... 详细信息

将构建的营养期杀虫蛋白基因vip83表达质粒pBMB2 32 8和含杀虫晶体蛋白基因(cry1Ac1 0或cry1Ca)质粒同时电转化无质粒突变株BMB1 71并双抗筛选。经PCR特异引物扩增验证 ,分别得到含cry1Ac1 0和vip83、cry1Ca和vip83的双基因重组菌BMB2 830 1 71和BMB2 882 1 71。用单基因重组菌作对照 ,分别测定了营养期杀虫蛋白Vip83与杀虫晶体蛋白Cry1Ac1 0和Cry1Ca两组蛋白对 3种重要鳞翅目害虫毒力。经统计分析 ,结果表明两组杀虫蛋白Vip83与Cry1Ac1 0和Vip83与Cry1Ca之间对棉铃虫均存在拮抗作用 ,对甜菜夜蛾协同作用不明显 ;但对小菜蛾前者协同作用不明显 ,而后者则有增效作用 ,其共毒系数为32 6。双基因的遗传稳定性检测表明这种正负协同关系具有一定的分子遗传稳定性。

关键词：苏云金芽胞杆菌营养期杀虫蛋白杀虫晶体蛋白协同作用

Digital light processing (DLP)-based (bio)printing strategies for tissue modeling and regeneration

在线全文

学校读者我要写书评

暂无评论

聚集体(英文)

聚集体(英文) 2023年第2期4卷 42-61页

作者： Hongbin Li jiliang Dai Zixuan Wang Heshan Zheng Wanlu Li Mian Wang Feng Cheng MIIT Key Laboratory of Critical Materials technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbinP.R.China college of Light Industry and Textile Qiqihar UniversityQiqiharP.R.China biomanufacturing Center Department of Mechanical Engineering Tsinghua UniversityBeijingP.R.China School of biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong UniversityShanghaiP.R.China Institute for Regenerative Medicine Shanghai East HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Science and TechnologyTongji UniversityShanghaiP.R.China

Digital light processing(DLP)-based bioprinting technology has recently aroused considerable concerns as a strategy to deliver biomedical materials and/or specific cells to create sophisticated structures for various tissue modeling and *** this review,we display a concise introduction of DLP bioprinting,and a further discussion on the design and manufacture of DLP(bio)printer with varied bioinks and their biomedical applications toward drug screening,disease modeling,tissue repair,and regenerative ***,the advantages,challenges,and perspectives of the DLP printing platforms are *** is believed that DLP bioprinting will play a decisive role in the field of tissue model and regenerative medicine,mainly due to its time-efficient,higher resolution,and amenability to automation for various tissue needs.

关键词： disease modeling DLP bioprinting drug screening tissue modeling

紫色土植物促生菌筛选及其复合菌剂对生姜种苗的促生效应

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国蔬菜 2018年第6期 54-59页

作者：申鸿吴波李洪海刘奕清吴兴文杨星勇西南大学资源环境学院重庆400715 重庆天沛生姜研究院有限公司重庆市生姜资源利用工程技术研究中心重庆402160 农业部沼气科学研究所四川成都610041 重庆文理学院特色植物产业协同创新中心重庆402160 重庆市梁平区经济作物技术推广站重庆405200 重庆师范大学生命科学学院重庆401331

从轮作蔬菜紫色土中筛选出3株不同的植物促生菌(PGPB)菌株,分别为具自生固氮能力的解淀粉芽孢杆菌(Bacillus amyloliquefaciens GN03)、具溶磷作用的短小芽孢杆菌(Bacillus pumilus RP01)和具解钾作用的环状芽孢杆菌(Bacillus circulans... 详细信息

从轮作蔬菜紫色土中筛选出3株不同的植物促生菌(PGPB)菌株,分别为具自生固氮能力的解淀粉芽孢杆菌(Bacillus amyloliquefaciens GN03)、具溶磷作用的短小芽孢杆菌(Bacillus pumilus RP01)和具解钾作用的环状芽孢杆菌(Bacillus circulans JK02),将3种菌株配制成复合菌剂进行生姜种苗盆栽试验。结果表明,接种牛粪-拌施复合菌剂处理总体表现最优,与牛粪-拌施市售生物菌肥处理相比,根际细菌自生固氮菌、溶磷菌和解钾菌数量显著增高了1 674.1%、8 709.1%和225.2%;土壤速效磷、速效钾含量显著提高了87.5%和23.0%;姜苗分蘖数、叶面积和地上部、地下部干质量分别提高了40.0%、45.9%和28.6%、25.0%。应用适宜的PGPB复合菌剂对紫色土微生态环境和生姜种苗的生长有较好的促进效应。

关键词：生姜种苗紫色土植物促生菌菌种筛选盆栽试验促生效应

陇东黄土高原地区杏园土壤综合肥力对人工生草模式的响应及其环境解释

在线全文

学校读者我要写书评

暂无评论

干旱地区农业研究 2018年第4期36卷 29-39页

作者：王根旺宋曦甘肃省高校陇东生物资源保护与利用省级重点实验室甘肃庆阳745000 陇东学院生命科学与技术学院甘肃庆阳745000 陇东学院农林科技学院甘肃庆阳745000

为了揭示陇东黄土高原地区杏园土壤综合肥力对3种不同人工生草管理模式(模式A:杏树+黄花菜;模式B:杏树+紫花苜蓿;模式C:杏树+黄花菜+紫花苜蓿)及对照组CK(杏树+清耕)的响应机制,采用常规方法测定了土壤理化性质、酶活性及土壤微生物群... 详细信息

为了揭示陇东黄土高原地区杏园土壤综合肥力对3种不同人工生草管理模式(模式A:杏树+黄花菜;模式B:杏树+紫花苜蓿;模式C:杏树+黄花菜+紫花苜蓿)及对照组CK(杏树+清耕)的响应机制,采用常规方法测定了土壤理化性质、酶活性及土壤微生物群落多样性等14项土壤环境指标。结果表明:土壤碱性磷酸酶和转化酶活性分别在模式A和C下活性最高,分别为18.18±0.51μg·g^(-1)·h^(-1)和213.15±3.69μg·g^(-1)·h^(-1),而土壤脲酶则在模式B和C中活性最高,分别为25.52±0.79μg·g^(-1)·h^(-1)和26.44±0.57μg·g^(-1)·h^(-1),土壤速效钾和速效磷含量在3个模式间无显著差异,但模式B(36.46±1.84 mg·kg^(-1))的土壤碱解氮含量最高;4种土壤微生物群落多样性指数在不同生草模式下均呈模式C>模式B>模式A>CK,其中Shannon-Wiener指数和Pielou均匀度在模式B和模式C之间无显著差异。NMDS排序结果表明,影响杏树林不同生草模式土壤肥力差异的最主要环境因子是Patrick丰富度指数(r^2=0.8489)和土壤脲酶(r^2=0.8111)。方差分解结果表明,土壤微生物学特性、酶活性及理化特性的总叠加效应共同解释杏树根际土壤综合肥力变异的79%,而土壤酶活性与微生物学特性是影响土壤肥力变异的主要环境因子(41%),土壤理化特性(3%)的独立效应对土壤肥力的影响要明显低于土壤酶活性(15%)与土壤微生物学特性(13%)。

关键词：杏园人工生草管理模式土壤综合肥力陇东黄土高原

变温处理对百合生长发育影响及刺足根螨防治效果

在线全文

学校读者我要写书评

暂无评论

扬州大学学报（农业与生命科学版） 2021年第1期42卷 87-91页

作者：吴沈忠陆继亮李航段枫姜茂成江振威何燕红华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/农业农村部华中都市农业重点实验室武汉430070 云南省花卉技术培训推广中心昆明650224 南京卉域农业科技有限公司南京210043

以"索邦"百合(Lilium spp.)的休眠种球为材料,经不同温度和时间的温水浸泡处理,观测百合种球的发芽率、出苗率和营养生长期、盛花期的形态指标以及鳞茎中刺足根螨(Rhizoglyphus echinopus)的发生情况,探究变温处理对百合生长发育的影响... 详细信息

以"索邦"百合(Lilium spp.)的休眠种球为材料,经不同温度和时间的温水浸泡处理,观测百合种球的发芽率、出苗率和营养生长期、盛花期的形态指标以及鳞茎中刺足根螨(Rhizoglyphus echinopus)的发生情况,探究变温处理对百合生长发育的影响和刺足根螨的防治效果。结果表明:百合种球以48℃温水浸泡3 min,取出冷却,连续3次重复上述步骤后,再以45℃温水浸泡30 min的处理方式能有效防治刺足根螨的发生和危害,且变温处理能促进百合的生长。

关键词：百合刺足根螨变温处理生长发育