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Deguelin regulates nuclear pore complex proteins Nup98 and Nup88 in U937 cells in vitro1

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Acta Pharmacologica Sinica 2005年第10期26卷 1265-1273页

作者： Hong-li LIU~2, Yan CHEN~(2,3), Guo-hui CUI~2, Qiu-ling WU~2, Jing HE~2, Wei-hua CHEN~2, Jian-feng ZHOU~4 ~2 Department of Hematology, Union Hospital, Tongji medical college, Huazhong university of science and Technology, Wuhan 430022 ~4 Department of Hematology, Tongji Hospital, Tongji medical college, Huazhong university of science and Technology, Wuhan 430030, china Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro. Methods: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. Results: The prolif eration of U937 cells was inhibited in the deguelin-treated group, with a 24-h IC50value of 21.61 nmol/L and a 36-h IC50value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G0/G1phase, whereas cells accumulated in the S and G2/M phases. Nup88 and Nup98 were found on both the nuclar and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. Conclusion: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G2/M phases, so that the proportion of cells in the G0/G1phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.

关键词： deguelin U937 cells nucleoporin Nup98 Nup88

DYNAMIC CHARACTERISTICS OF LIQUID SLOSHING IN A RECTANGULAR TANK UNDER LOW GRAVITY

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Journal of Hydrodynamics 2006年第2期18卷 170-176页

作者： CHENG Xu-duo HU Mei-zhu LIU Zhi-yun School of Food science and Engineering Nanjing University of Finance and Economics Nanjing 210003 China Department of Physics Anqing Teachers College Anqing 246011 China School of Food science and Engineering Nanjing University of Finance and Economics Nanjing 210003 China

In this paper, the dynamics of liquid sloshing in a rectangular tank under low gravity was investigated. The basic frequencies and the velocity potentials of sloshing of liquid were obtained by expansion of the Fourier series. The characteristics of force and moment of the liquid acting on a rectangular tank were analyzed. The equivalent mechanical models of spring-vibrator-damper of sloshing of liquid were established with mechanical equivalent principle and numerical results were given. The numerical results show that the equivalent sloshing mass increases with the increase of gravity, and that the basic frequency and the equivalent sloshing mass increase with the increase of the liquid depth.

关键词： low gravity liquid sloshing equivalent mechanical model

MODEL OF CALCULATING WATER QUANTITY NEEDED TO DILUTE AND PURIFY POLLUTANTS IN RIVER NETWORK ANDITS APPLICATIONS

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Journal of Hydrodynamics 2005年第4期17卷 418-428页

作者： WANG Chao WANG Pei-fang Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Hohai University Nanjing 210098 China college of Environmental science and Engineering Hohai University Nanjing 210098 China

Based on the analysis of dilution capacity and self-purification capacity of water body, the dilution, dispersion, entrapping and purification principles of pollutants in river system at river network area were discussed in this paper. Also, the one and two dimensional models of water quantity needed for improving water environment quality and pollutant concentrations were developed for rivers and lakes respectively. The calculation method for the quantity of water transfer was given and the forecasting evaluation of the effect of water transfer was carried out. It was took the project, water transfer from Yangtze River to improve the water quality of rivers in Zhangjiagang City, as an example, and changing principles of water quantity and quality were observed in rivers and lakes through site water transfer experiments. The theory of estimating parameters in inverse problem was used to determine parameters in water quantity and quality models. The water quantity and quality coupled models in river system were applied to calculate the minimal water transfer quantity. The theoretical and technical support for the improvement of water environmental quality in Zhangjiagang City and the project ＂water transfer form Yangtze River to Taihu Lake＂ were provided.

关键词： river network dilution purification mathematical models site observation

A PCA-based method for ancestral informative markers selection in structured populations

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science china(Life sciences) 2009年第10期52卷 972-976页

作者： ZHANG Feng1,3, ZHANG Lei1,3 & DENG Hong-Wen1,2,3 1 Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Molecular Genetics, School of Life science and Technology, Xi’an Jiaotong university, Xi’an 710049, china 2 Institute of Bioscience and Biotechnology, School of science, Beijing Jiaotong university, Beijing 100044, china 3 Departments of Orthopedic Surgery and basic medical science, School of Medicine, university of Missouri-Kansas City, Kansas City, MO 64108, USA Key Laboratory of Biomedical Information Engineering of Ministry of Education Institute of Molecular Genetics School of Life Science and Technology Xi'an Jiaotong University Xi'an 710049 China.

Identification of population structure can help trace population histories and identify disease genes. Structured association (SA) is a commonly used approach for population structure identification and association mapping. A major issue with SA is that its performance greatly depends on the informa-tiveness and the numbers of ancestral informative markers (AIMs). Present major AIM selection meth-ods mostly require prior individual ancestry information, which is usually not available or uncertain in practice. To address this potential weakness, we herein develop a novel approach for AIM selection based on principle component analysis (PCA), which does not require prior ancestry information of study subjects. Our simulation and real genetic data analysis results suggest that, with equivalent AIMs, PCA-based selected AIMs can significantly increase the accuracy of inferred individual ancestries compared with traditionally randomly selected AIMs. Our method can easily be applied to whole genome data to select a set of highly informative AIMs in population structure, which can then be used to identify potential population structure and correct possible statistical biases caused by population stratification.

关键词： population structure principle component analysis ancestral informative markers

Autoantibody against Cardiac β1-Adrenoceptor Induces Apoptosis in Cultured Neonatal Rat Cardiomyocytes

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 443-449页

作者： Yan GAO~1 Hui-Rong LIU~1 Rong-Rui ZHAO~1 Jian-Ming ZHI~(2*) 1 Department of Cardiovascular Physiology Shanxi medical university,Taiyuan 030001,china 2 Department of Physiology,Shanghai Jiaotong university,college of basic Medicine,Shanghai 200025,china Department of Cardiovascular Physiology Shanxi Medical UniversityTaiyuan 030001 China Department of Physiology Shanghai Jiaotong University College of Basic MedicineShanghai 200025 China

To clarify whether apoptosis is involved in the injury processes induced by autoantibodyagainst cardiac β1-adrenoceptor,we investigated the biological and apoptotic effects of antibodies on culturedneonatal rat *** rats were immunized with peptides corresponding to the second extra-cellular loop of the β1-adrenoceptor to induce the production of anti-β1-adrenoceptor antibodies in the ***（Ig）G in the sera was detected using synthetic antigen enzyme-linked immunosorbentassay and purified using the diethylaminoethyl cellulose ion exchange *** of cardiomyo-cytes was evaluated using agarose gel electrophoresis and flow *** results showed that thepositive serum IgG greatly increased the beating rates of cardiomyocytes and showed an"agonist-like"***,positive serum IgG induced cardiomyocyte apoptosis after treatment with β1-adrenoceptor overstimulation for *** effects of monoclonal antibody against β-adrenoceptor werealso found to be similar to those of positive serum *** was suggested that the autoantibody could inducecardiomyocyte apoptosis by excessive stimulation of β1-adrenoceptor.

关键词： β1-adrenoceptor rat cardiomyocyte autoantibody apoptosis

Diverse Effects of L-arginine on Cardiac Function of Rats Subjected to Myocardial Ischemia and Reperfusion in vivo

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 201-207页

作者： Xiaoliang WANG~1 Feng LIANG~2 Xiangying JIAOI Lei LIU~1 Xiaojie BAI~1 Meixia LI~1 Jianmin ZHI~3 Huirong LIU~1* 1 Department of Physiology,Shanxi medical university,Taiyuan 030001,china 2 Department of Cardiothoracic Surgery,General Hospital of Taiyuan Iron and Steel Company Taiyuan 030003,china 3 Department of Physiology.Shanghai Jiaotong university.college of basic Medicine,Shanghai 200025,china Department of Physiology Shanxi Medical UniversityTaiyuan 030001 China Department of Cardiothoracic Surgery General Hospital of Taiyuan Iron and Steel CompanyTaiyuan 030003 China Department of Physiology Shanghai Jiaotong University College of Basic MedicineShanghai 200025 China

In vivo administration of L-arginine at different time points during the course of myocardialischemia and reperfusion（MI/R）has been shown to differentially regulate postischemic *** is one of the most important indexes used to judge the degree of myocardial *** presentstudy attempted to determine whether in vivo administration of L-arginine at different stages of MI/R has adiverse influence on cardiac function of ischemic reperfused hearts and,if so,to investigate the *** adult rats were subjected to 30 min myocardial ischemia followed by 5 h *** L-arginine bolus was given either 10 min before and 50 min after reperfusion（early treatment）or3 h and 4 h after reperfusion（late treatment）.Early treatment with L-arginine markedly increased the leftventricular systolic pressure（LVSP）and dP/dtmax,and decreased myocardial nitrotyrosine *** strictcontrast,late treatment with L-arginine resulted in a significant decrease in LVSP and dP/dtmaxfrom 4 h to 5h after reperfusion,and increase in toxic peroxynitrite formation as measured by *** resultssuggest that the administration of L-arginine at different time points during the course of MI/R leads todiverse effects on cardiac *** supplementation decreased the nitrative stress and improvedleft ventricular ***,late treatment with L-arginine increased the formation of peroxynitriteand aggravated cardiac functional injury.

关键词： myocardial ischemia reperfusion cardiac function nitric oxide peroxynitrite

Sonic hedgehog protein promotes bone marrow-derived endothelial pro-genitor cell proliferation,migration and VEGF production via PI 3-kinase/Akt signaling pathways

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Acta Pharmacologica Sinica 2006年第6期27卷 685-693页

作者： Jin-rong FU~2 Wen-li LIU~(2,4)Jian-feng ZHOU~2 Han-ying SUN~2 Hui-zhen XU~2 Li LUO~2 Heng ZHANG~2 Yu-feng ZHOU~3 2 Department of Hematology.Tongji Hospital,Tongji medical college,Huazhong university of science and Technology,Wuhan 430030,china:3 Department of Pediatrics,Tongji Hospital,Tongji medical college,Huazhong university of science and Technology,Wuhan 430030,china Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effects of Sonic hedgehog(shh)protein on bone marrow-derived endothelial progenitor cells(BM-EPC)proliferation,migration and vascu-lar endothelial growth factor(VEGF)production,and the potential signaling path-ways involved in these ***:Bone marrow-derived Flk-1~+ cells wereenriched using the MACS system from adult Kunming mice and then BM-EPCwas cultured in gelatin-coated culture *** effects of shh N-terminal pep-tide on BM-EPC proliferation were evaluated using the MTT colorimetric *** migration was assayed using a modified Boyden chamber *** of VEGF was determined by ELISA and immunofluorescence *** potential involvement of PKC and PI3K signaling pathways was exploredusing selective inhibitor or Western ***:The proliferation,migrationand VEGF production in BM-EPC could be promoted by endogenous shh N-terminal peptide at concentrations of 0.1μg/mL to 10 μg/mL,and could be inhib-ited by anti-shh ***-mediated proliferation and migration in BM-EPCcould be partly attenuated by ***-PI3-kinase expression in newlyseparated BM-EPC was low,and it increased significantly when exogenous shhN-terminal peptide was added,but could be attenuated by anti-human/mouse shhN-terminal peptide ***,the inhibitor of the PI3-kinase,but notthe inhibitor of the PKC,significantly inhibited the shh-mediated proliferation,migration and VEGF ***:Shh protein can stimulate bonemarrow-derived BM-EPC proliferation,migration and VEGF production,whichmay promote neovascularization to ischemic *** results also suggeststhat the PI3-kinase/Akt signaling pathways are involved in the angiogenic effectsof shh.

关键词： mouse Sonic hedgehog protein endothelial cells stem cells protein kinase C 1-phosphatidylinositol 3-kinase vascular endothelial growth factor A cell proliferation emigration immigration

Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model

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Acta Pharmacologica Sinica 2007年第1期28卷 98-104页

作者： Cheng-liang ZHANG Xiao-lei ZOU Jia-bei PENG Ming XIANG~2 Department of Pharmacology college of Pharmacy Tongji medical college,Huazhong university of science and Technology,Wuhan 430030,china Department of Pharmacology College of Pharmacy Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effect and underlying mechanisms of immune-toleranceinduced by the adoptive transfer of bone marrow(BM)-derived dendritic cells(DC)in insulin-dependent diabetes mellitus(IDDM)***:The IDDMmodel was established by a low close of streptozotocin(STZ)in Balb/c *** subpopulations were generated from the BM cells with granulocyte-macroph-age colony-stimulating factor with or without *** purity and the Tcell stimulatory capability of DC were *** cells were used to modu-late autoimmune response in pre-diabetic *** glucose was examinedweekly;pancreas tissues were taken for histopathological analysis,and CD4~+ Tcells were isolated to detect lymphocyte proliferation by MTT assay and the ratioof CD4~+CD25+ T cells by fluorescence-activated cell sorting(FACS).The cytokinesecretion was determined by ELISA ***:Two DC subsets weregenerated from BM,which have phenotypes of mature DC(mDC)and immatureDC(iDC),*** level of blood glucose decreased significantly bytransferring iDC(P<0.01)rather than *** lymphocyte infiltration was ob-served in the islets,and pancreatic structure was *** vitro,proliferation oflymphocytes decreased and the proportion of CD4~+CD25~+ T cells increasedremarkably,compared with the mDC-treated groups(P<0.05),which were associ-ated with increased level of the Th2 cytokine and reduced level of the Th 1 cytokineafter iDC ***:Our data showed that iDC transfer was able toconfer protection to mice from STZ-induced *** immune-tolerance toIDDM may be associated with promoting the production of CD4~+CD25~+ T cellsand reducing regulatory Th2 responses in vivo.

关键词： dendritic cells insnlin-dependent diabetes mellitus streptozotocin immune tolerance regulatory T cells

Experimental study on antitumor effect of arsenic trioxide in combination with cisplatin or doxorubicin on hepatocellular carcinoma

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World Journal of Gastroenterology 2001年第5期7卷 702-705页

作者： Wei Wang~1 Shu-Kui Qin~1 Bao-An Chen~2 Hui-Ying Chen~1 1 Chinese PLA Cancer Center,Chinese PLA 81 Hospital,nanjing 210002,Jiangshu Province,china2 Affliliated Zhongda Hospital of southeast university medical college,nanjing 210087,Jiangsu Province,china Chinese PLA Cancer Center Chinese PLA 81 Hospital Nanjing 210002 Jiangshu Province China.

INTRODUCTIONThe main component of a traditional Chinese drug 'Pishuang'. arsenic trioxide (As2O3), has obviously selective anti-tumor effect on human hepatocellular carcinoma (HCC)in both in vitro and in vivo studies[1-5]. Due to limited effectiveness when any anti-carcinogen is used alone and obviously increased toxicity when the dose is raised, there is no exception for As2O3. Furthermore, combined chemotherapy contributes to improve therapeutic effectiveness, disperse toxicity and surmount drug-resistance,in which the combination of traditional Chinese and modern medicine has more advantages and characteristics. As a result,we made an experimental study on anti-tumor effect of As2O3in combination with cisplantin (PDD) or doxorubicin (ADM)on HCC. to investigate the possibility of AS2O3 in combination with PDD or ADM and nature of interaction between them,and to provide experimental basis for clinical application.

关键词： Animals Antineoplastic Agents Antineoplastic Combined Chemotherapy Protocols Arsenicals Carcinoma, Hepatocellular Cisplatin Doxorubicin Female Humans Liver Neoplasms, Experimental Male Mice Mice, Inbred Strains Neoplasm Transplantation Oxides Research Support, Non-U.S. Gov't Tumor Cells, Cultured